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The map label for this gene is dnaG [H]

Identifier: 78221615

GI number: 78221615

Start: 435368

End: 437134

Strand: Direct

Name: dnaG [H]

Synonym: Gmet_0394

Alternate gene names: 78221615

Gene position: 435368-437134 (Clockwise)

Preceding gene: 78221614

Following gene: 78221616

Centisome position: 10.89

GC content: 62.31

Gene sequence:

>1767_bases
ATGTCACTGATCCCCGACGATAAGATCAGGGAGGTCCGGGAGCGGGCCTCGATTCTGGACGTGGTGTCCGACTACGTGAG
CCTCCGGAAGTCGGGAGCCAACTACCAGGGGCTTTGCCCCTTCCATGGCGAGAAGACCCCTTCCTTCAACGTGAATCCGG
CCCGGGGGATCTTCCACTGCTTCGGTTGCGGCGTGGGGGGGAATGCGATTACGTTCATCGCCAGGATGGAGGGGCTCTCG
TTCCCCGAGGCGGTGAAGTTTCTTGCCAAGCGGGTGGGGGTTGAGATCGAGGATCGTCCCGTATCCGCTGCGGAAAAGCG
GCGCCTGGACGAACGGGAGGAACTCCACGGTATCATGGATCTGGCCGTGGGGTTCTACCGCGAGGCCCTCGAACGGAGCG
AGGAGGGGGGGACGGCGCGTCGCTATCTGGAAGGACGTGGTGTGGATCGGGCCACGGCAGAGGCGTACTGCCTCGGCTTT
GCCCCGGACCGCTGGGACTTCTTGGCCCGTCATCTCCAGCATCGCTCGGTGCCGCTGGAACTGGCGGAGAAGCTAGGTCT
TGTGAGACGTCGGGACGGAGGCGGGTTCTATGACACCTTCCGAAACCGGCTTCTCTTTACCATCACCGACATCCATGGCC
GTGCCATCGGGTTCGGCGGCAGGGTTCTCGACGACTCGCTACCCAAATACATCAATTCTCCCGAATCGCCCATCTATCAC
AAGAGCGAGGTCCTCTTCGGCGTCTCCCTCGCCAAGGGAGCCATGCGGGAGACGGCGAGCGCCATCGTGGTGGAGGGGTA
TTTTGACCATTTGGCCCTCTACCAGGCAGGGGTTAGGAACGTGGTCGCCACCTGCGGTACGGCCCTCACCGAGGGGCATA
TCAAGCTTCTGAAGCGCTACGCGGGGAATGTCTATACCCTGTTCGACGCCGACAGCGCCGGACGGAAAGCGACGCTGCGG
GCCATGGACCTCTGCCTCGATGCCGGATTCCCCGCCCATGTGGTGGAGCTGACCGCCGGGGAGGATCCGGACAGCTTCAT
CCGGAAAGAGGGTGCCGAGACGTTTTCGGCCCGTCTGGCAAAAGCGCGTCCGGTGGTTGATTATTTCTTTCGTCAGCTGG
TGCGGGACGAGGATACCGGTACCGTCGAGGGGAAGGTGAAGGTCATCGACGAGATGGCCCCGCGGCTTGCAAGGGTCTCC
AATGCCATCGAGCGGGAACTCTATGTGCGGGAAATCTCCCGGGTTCTCGGTGTCGATGTTCGCTCTTTGGCGCGGAAGAT
CGGTGGTTCCTCGTCCCCTGCTGCTCCCCGGGACGAGAGGGCTCCCGCGCAGTCTCGCCGGAAAAAGGGTGCCACCCCCG
AGGAGATGCTCCTCTCGCTCATGGGGCGCTATCCTGAGGTGGCTGAACGGGTAAGGAACCACGACCCTGCGCGTATCTTC
GGCCCGCAGTTCCTTCCCGTCGCCGAGGCAATCCTGGAGCGTATCGGAGCCGGGAAGCCAGTCGAATGGAGCGAGATTCT
CGATCTGGTGGAAGGGGGAGAGGAACGGGGCCGTATCGCCGCATTTCTTGTCAACGACGACCATCTGGCCGACGTTGATG
TCCACAAGGCCTTTGACCAGTGCCGTGCGGCTCTGGAGCGGAACTCTCTCGGCCGGATGAAGGAGTTGGCTCGGGAACTG
GCGGCGACCGACCCCGATAGTCCTCGCTACCGGGATCTCCTGGCTGAGATTGATGCCTTGCGAAACCGTAAATCGCAGTT
ATCTTAA

Upstream 100 bases:

>100_bases
GGCTCTGCCCGGGCCCTCGCCGACTGCGGGAAGGACATTGTGACTGGTTGGCGAAAAAATGCTGTTGAGAAACGGCCTGC
CGGAGAAAATCGGACTAAAG

Downstream 100 bases:

>100_bases
TAAGAACAGCGTGCAATGAGGTGAATTTGATGGCCAAGAAAAGTTCGGACGAAGTTAAGCAGCAGCTGGTGGACATCGGC
AAGGAGAAGGGATTCCTTAC

Product: DNA primase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 588; Mature: 587

Protein sequence:

>588_residues
MSLIPDDKIREVRERASILDVVSDYVSLRKSGANYQGLCPFHGEKTPSFNVNPARGIFHCFGCGVGGNAITFIARMEGLS
FPEAVKFLAKRVGVEIEDRPVSAAEKRRLDEREELHGIMDLAVGFYREALERSEEGGTARRYLEGRGVDRATAEAYCLGF
APDRWDFLARHLQHRSVPLELAEKLGLVRRRDGGGFYDTFRNRLLFTITDIHGRAIGFGGRVLDDSLPKYINSPESPIYH
KSEVLFGVSLAKGAMRETASAIVVEGYFDHLALYQAGVRNVVATCGTALTEGHIKLLKRYAGNVYTLFDADSAGRKATLR
AMDLCLDAGFPAHVVELTAGEDPDSFIRKEGAETFSARLAKARPVVDYFFRQLVRDEDTGTVEGKVKVIDEMAPRLARVS
NAIERELYVREISRVLGVDVRSLARKIGGSSSPAAPRDERAPAQSRRKKGATPEEMLLSLMGRYPEVAERVRNHDPARIF
GPQFLPVAEAILERIGAGKPVEWSEILDLVEGGEERGRIAAFLVNDDHLADVDVHKAFDQCRAALERNSLGRMKELAREL
AATDPDSPRYRDLLAEIDALRNRKSQLS

Sequences:

>Translated_588_residues
MSLIPDDKIREVRERASILDVVSDYVSLRKSGANYQGLCPFHGEKTPSFNVNPARGIFHCFGCGVGGNAITFIARMEGLS
FPEAVKFLAKRVGVEIEDRPVSAAEKRRLDEREELHGIMDLAVGFYREALERSEEGGTARRYLEGRGVDRATAEAYCLGF
APDRWDFLARHLQHRSVPLELAEKLGLVRRRDGGGFYDTFRNRLLFTITDIHGRAIGFGGRVLDDSLPKYINSPESPIYH
KSEVLFGVSLAKGAMRETASAIVVEGYFDHLALYQAGVRNVVATCGTALTEGHIKLLKRYAGNVYTLFDADSAGRKATLR
AMDLCLDAGFPAHVVELTAGEDPDSFIRKEGAETFSARLAKARPVVDYFFRQLVRDEDTGTVEGKVKVIDEMAPRLARVS
NAIERELYVREISRVLGVDVRSLARKIGGSSSPAAPRDERAPAQSRRKKGATPEEMLLSLMGRYPEVAERVRNHDPARIF
GPQFLPVAEAILERIGAGKPVEWSEILDLVEGGEERGRIAAFLVNDDHLADVDVHKAFDQCRAALERNSLGRMKELAREL
AATDPDSPRYRDLLAEIDALRNRKSQLS
>Mature_587_residues
SLIPDDKIREVRERASILDVVSDYVSLRKSGANYQGLCPFHGEKTPSFNVNPARGIFHCFGCGVGGNAITFIARMEGLSF
PEAVKFLAKRVGVEIEDRPVSAAEKRRLDEREELHGIMDLAVGFYREALERSEEGGTARRYLEGRGVDRATAEAYCLGFA
PDRWDFLARHLQHRSVPLELAEKLGLVRRRDGGGFYDTFRNRLLFTITDIHGRAIGFGGRVLDDSLPKYINSPESPIYHK
SEVLFGVSLAKGAMRETASAIVVEGYFDHLALYQAGVRNVVATCGTALTEGHIKLLKRYAGNVYTLFDADSAGRKATLRA
MDLCLDAGFPAHVVELTAGEDPDSFIRKEGAETFSARLAKARPVVDYFFRQLVRDEDTGTVEGKVKVIDEMAPRLARVSN
AIERELYVREISRVLGVDVRSLARKIGGSSSPAAPRDERAPAQSRRKKGATPEEMLLSLMGRYPEVAERVRNHDPARIFG
PQFLPVAEAILERIGAGKPVEWSEILDLVEGGEERGRIAAFLVNDDHLADVDVHKAFDQCRAALERNSLGRMKELARELA
ATDPDSPRYRDLLAEIDALRNRKSQLS

Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis [H]

COG id: COG0358

COG function: function code L; DNA primase (bacterial type)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Escherichia coli, GI1789447, Length=472, Percent_Identity=35.8050847457627, Blast_Score=285, Evalue=4e-78,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007693
- InterPro:   IPR013264
- InterPro:   IPR019475
- InterPro:   IPR006295
- InterPro:   IPR006171
- InterPro:   IPR002694 [H]

Pfam domain/function: PF10410 DnaB_bind; PF01751 Toprim; PF08275 Toprim_N; PF01807 zf-CHC2 [H]

EC number: 2.7.7.-

Molecular weight: Translated: 65271; Mature: 65140

Theoretical pI: Translated: 7.17; Mature: 7.17

Prosite motif: PS00041 HTH_ARAC_FAMILY_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLIPDDKIREVRERASILDVVSDYVSLRKSGANYQGLCPFHGEKTPSFNVNPARGIFHC
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHH
FGCGVGGNAITFIARMEGLSFPEAVKFLAKRVGVEIEDRPVSAAEKRRLDEREELHGIMD
HCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHCCHHHHHHHHHHH
LAVGFYREALERSEEGGTARRYLEGRGVDRATAEAYCLGFAPDRWDFLARHLQHRSVPLE
HHHHHHHHHHHHCCCCCHHHHHHHCCCCCHHHHHHEEECCCCCHHHHHHHHHHHCCCCHH
LAEKLGLVRRRDGGGFYDTFRNRLLFTITDIHGRAIGFGGRVLDDSLPKYINSPESPIYH
HHHHHCHHEECCCCCHHHHHHCCEEEEEEECCCCEECCCCCHHHHHHHHHHCCCCCCCCC
KSEVLFGVSLAKGAMRETASAIVVEGYFDHLALYQAGVRNVVATCGTALTEGHIKLLKRY
CHHHHHHHHHHHHHHHHHHHHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AGNVYTLFDADSAGRKATLRAMDLCLDAGFPAHVVELTAGEDPDSFIRKEGAETFSARLA
CCCEEEEEECCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHH
KARPVVDYFFRQLVRDEDTGTVEGKVKVIDEMAPRLARVSNAIERELYVREISRVLGVDV
HCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
RSLARKIGGSSSPAAPRDERAPAQSRRKKGATPEEMLLSLMGRYPEVAERVRNHDPARIF
HHHHHHHCCCCCCCCCCCCCCCHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHCCCCCEEC
GPQFLPVAEAILERIGAGKPVEWSEILDLVEGGEERGRIAAFLVNDDHLADVDVHKAFDQ
CCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCEEEEEECCCCCCCHHHHHHHHH
CRAALERNSLGRMKELARELAATDPDSPRYRDLLAEIDALRNRKSQLS
HHHHHHHCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SLIPDDKIREVRERASILDVVSDYVSLRKSGANYQGLCPFHGEKTPSFNVNPARGIFHC
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHH
FGCGVGGNAITFIARMEGLSFPEAVKFLAKRVGVEIEDRPVSAAEKRRLDEREELHGIMD
HCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHCCHHHHHHHHHHH
LAVGFYREALERSEEGGTARRYLEGRGVDRATAEAYCLGFAPDRWDFLARHLQHRSVPLE
HHHHHHHHHHHHCCCCCHHHHHHHCCCCCHHHHHHEEECCCCCHHHHHHHHHHHCCCCHH
LAEKLGLVRRRDGGGFYDTFRNRLLFTITDIHGRAIGFGGRVLDDSLPKYINSPESPIYH
HHHHHCHHEECCCCCHHHHHHCCEEEEEEECCCCEECCCCCHHHHHHHHHHCCCCCCCCC
KSEVLFGVSLAKGAMRETASAIVVEGYFDHLALYQAGVRNVVATCGTALTEGHIKLLKRY
CHHHHHHHHHHHHHHHHHHHHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AGNVYTLFDADSAGRKATLRAMDLCLDAGFPAHVVELTAGEDPDSFIRKEGAETFSARLA
CCCEEEEEECCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHH
KARPVVDYFFRQLVRDEDTGTVEGKVKVIDEMAPRLARVSNAIERELYVREISRVLGVDV
HCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
RSLARKIGGSSSPAAPRDERAPAQSRRKKGATPEEMLLSLMGRYPEVAERVRNHDPARIF
HHHHHHHCCCCCCCCCCCCCCCHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHCCCCCEEC
GPQFLPVAEAILERIGAGKPVEWSEILDLVEGGEERGRIAAFLVNDDHLADVDVHKAFDQ
CCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCEEEEEECCCCCCCHHHHHHHHH
CRAALERNSLGRMKELARELAATDPDSPRYRDLLAEIDALRNRKSQLS
HHHHHHHCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups; Nucleotidyltransferases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]