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The map label for this gene is hupB [H]

Identifier: 78221576

GI number: 78221576

Start: 397964

End: 398248

Strand: Direct

Name: hupB [H]

Synonym: Gmet_0355

Alternate gene names: 78221576

Gene position: 397964-398248 (Clockwise)

Preceding gene: 78221575

Following gene: 78221577

Centisome position: 9.96

GC content: 59.3

Gene sequence:

>285_bases
ATGACCAAAGCTGAACTTGTGGATGCCGTAGCGAAATCTGCCAACCTCACCAAGGCTGCCGCCGACAAGGCGGTGGGCGC
CGTAATCAGTGCCGTGAGCGATGCCCTCAAGAAGGGCGACAAAGTGACCCTGGTTGGATTCGGGAGCTTCGAGGTGTCCA
CCCGCAAGGCCCGTACCGGCCGTAACCCCCAAACCGGCAAAGAGATCAAGATCGCTGCTGCCAAGGTTCCCAAGTTCCGC
CCGGGCAAGGCTCTCAAGGACGCCGTTGCCGCCAAGAAAAAATAA

Upstream 100 bases:

>100_bases
CGACCCATGAAAAAATTATTTTTCTCCAAATAATCCCTTGCAATGTGGAAATTTCTGTCTATAGTATTCGCCAGAATTAC
CTTAACAAGGAGGGTTGATC

Downstream 100 bases:

>100_bases
TTTCCGCGCGTCCCCCATGGGGAGACGTTGCTGATATAATTATACATGTGATAAGAAACCCCGCCGGTGCGGGGTTTTTT
CATTGCTGAACTCTTTGGGG

Product: histone-like DNA-binding protein

Products: NA

Alternate protein names: HU-1; NS1 [H]

Number of amino acids: Translated: 94; Mature: 93

Protein sequence:

>94_residues
MTKAELVDAVAKSANLTKAAADKAVGAVISAVSDALKKGDKVTLVGFGSFEVSTRKARTGRNPQTGKEIKIAAAKVPKFR
PGKALKDAVAAKKK

Sequences:

>Translated_94_residues
MTKAELVDAVAKSANLTKAAADKAVGAVISAVSDALKKGDKVTLVGFGSFEVSTRKARTGRNPQTGKEIKIAAAKVPKFR
PGKALKDAVAAKKK
>Mature_93_residues
TKAELVDAVAKSANLTKAAADKAVGAVISAVSDALKKGDKVTLVGFGSFEVSTRKARTGRNPQTGKEIKIAAAKVPKFRP
GKALKDAVAAKKK

Specific function: Histone-like DNA-binding protein which is capable of wrapping DNA to stabilize it, and thus to prevent its denaturation under extreme environmental conditions [H]

COG id: COG0776

COG function: function code L; Bacterial nucleoid DNA-binding protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial histone-like protein family [H]

Homologues:

Organism=Escherichia coli, GI1786644, Length=89, Percent_Identity=64.0449438202247, Blast_Score=117, Evalue=1e-28,
Organism=Escherichia coli, GI1790433, Length=89, Percent_Identity=58.4269662921348, Blast_Score=108, Evalue=7e-26,
Organism=Escherichia coli, GI1787141, Length=88, Percent_Identity=36.3636363636364, Blast_Score=77, Evalue=2e-16,
Organism=Escherichia coli, GI1788005, Length=89, Percent_Identity=39.3258426966292, Blast_Score=72, Evalue=5e-15,

Paralogues:

None

Copy number: 860 Molecules/Cell In: Growth-Phase, Minimal Media (Based on E. coli). 2040 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000119
- InterPro:   IPR020816
- InterPro:   IPR010992 [H]

Pfam domain/function: PF00216 Bac_DNA_binding [H]

EC number: NA

Molecular weight: Translated: 9693; Mature: 9562

Theoretical pI: Translated: 11.16; Mature: 11.16

Prosite motif: PS00045 HISTONE_LIKE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
0.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKAELVDAVAKSANLTKAAADKAVGAVISAVSDALKKGDKVTLVGFGSFEVSTRKARTG
CCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHCC
RNPQTGKEIKIAAAKVPKFRPGKALKDAVAAKKK
CCCCCCCEEEEEECCCCCCCCCHHHHHHHHHCCC
>Mature Secondary Structure 
TKAELVDAVAKSANLTKAAADKAVGAVISAVSDALKKGDKVTLVGFGSFEVSTRKARTG
CHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHCC
RNPQTGKEIKIAAAKVPKFRPGKALKDAVAAKKK
CCCCCCCEEEEEECCCCCCCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8550432 [H]