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The map label for this gene is mrcA [H]

Identifier: 78221575

GI number: 78221575

Start: 395316

End: 397841

Strand: Direct

Name: mrcA [H]

Synonym: Gmet_0354

Alternate gene names: 78221575

Gene position: 395316-397841 (Clockwise)

Preceding gene: 78221573

Following gene: 78221576

Centisome position: 9.89

GC content: 62.43

Gene sequence:

>2526_bases
ATGACGCCTGCCAGCGGCAGACAGAACATTGACCCCCGGAATGTAAACCGGCGGAGAGACAAAAATCCATGGAAAGTTGT
CGTCATAACGCTCGTTACCCTTGGTGTTGTGGCCCTTCTCGCTGTGTTCGGCTATTTCACCTATCTTTACGCCTCCCTGC
CGAAGGTCGACCGTCTTGCCGACTACCGGCCTCCCATTGTTTCCCAGGTCTTTGGCGATGATGGCAGCCTTGTTGGGGAG
TTTTACCTGGAGCGGCGCATCGTCGTGCCGGTGGAGAAAATTCCCCGGAAGCTGATCCAGGCTTTCGTGGCGGCCGAGGA
TTCCAACTTCTACCAGCACAAGGGGCTCGATTACCTGGGGATCGCCCGGGCCGCGGTGAAAAACCTCCTCGCTATGCGGA
AGAAGGAGGGGGCGTCCACCATCACCCAGCAGGTGGCGAAGTCGATGCTTCTCACCTCCGAGAAGAGTTTTGCCCGCAAG
TTCAAGGAGGCGATTCTTGCCACTCGCATGGAGAAGATGCTCTCCAAGGACGACATCCTCTACATCTACCTGAACCAGAT
CTATCTGGGGGGAGGCGCCTACGGGGTCCAGGTGGCTGCCGAGACCTACTTCGGCAAGAACGTGGAGCAGCTGAACCTGG
CCGAGATGGCCATGCTCGCCGGGCTCCCCAAGGCCCCCAACTCCTACTCTCCCATCAAGCACCTGGACCGGGCCCGGGAA
CGGCAGGCCTACGTCCTTGAGCGGATGGTAAGGGAGGGGTACGTGACCCAGGCCGAGGCGGACCACGCCCGGGCGACCCC
CATCGTGGTCAGGTCCATGAAGAAGGTAAACAGCGAGCAGTCGGCCTATTTCCTTGAGCATATCCGGATCAAGCTGGAGG
AGACCTATGGGATGGACCGGCTCTACAAGGAAGGGCTGAAGATCTACACCACCATGAATGCCGAGATGCAGAAGGGGGCC
TTTGATGCGGTGGTGAACGGCCTGAAAAACGTGGACAAGCGCCAGGGGTTCCGGGGAGCGGTCAAGTACCTTGGGGAAAC
CGAGGTGGATACCTTCAGTAAGAAAGTGGAAGACGGGATCGATACCGCGTCCCTCAAGCAGGGGAGCACCTACCAGGGGG
TCGTCACCGCCGTGGATCCGGCAAAGGGGGATGCAACCGTGCGGGTTGGAGACCGGACCGGCACCATCTCCCGCAAGAAC
ATGGCCTGGGCCGGCAAGGTGCAGCTTGTGAACGCCTGGGGCAAGCAGGACGGGAAAGGGAAGTCGCTCCCCTTGGGGAG
CGTTGTCTTCGTTTCGGTGCTCACCCCTGATGTGAACAAGCAGGGGGCCATCTTCTCCCTGGACCAGGAGCCCGAGGCCC
AGGCGGCGGTGGTGGCCCTTGATCCCCTGACCGGTGCCGTGAAGGCCATGGTTGGCGGGTATGATTTCCGCAGGAGCCAG
TTCAACCGGGCCGTCCAGGCAAAGCGGAATCCCGGCTCTGCCTTCAAGCCGATCATCTACGCCGCCGCCCTCGACAAGGG
GCTCACGGCGGCAACAACCTTCGACGACTCCCCCGTCGAATACGAGGGGGGCGGGGACAAGGCCTGGAAACCGAAAAACT
ACGACAACATCTATCGGGGCATCGTGACCATGCGCGAAGCCCTCACCAATTCCATCAACGTGGTGAGCGTCAAGATTCTG
GAACAGATCGGCGTCGGCGCTGCCATCGACTATGCCAAAAAACTCGGCATCACCTCCCCCCTGGCCGCCAACCTTACCCT
GGCCCTCGGTTCGTCCAGCGTGACCCCCCTTGAATTGACCTCCGCCTACGCCGTATTCGCCTCGGGAGGGTACCGGACAA
CGCCTTACTTCATCACCAAAGTGGAGGATGCCGACGGCAAGGTTTTGGAGGAGACGGCACCGCCGGTCATTCCGACCTTC
ACCAACCAGAGCACGGCCACCCTCACCAATGCAGACCTCCCACCCGCAACGGGCGCCGTTCCCGGGGGGGGAGCGGTACC
GGCCATTGCGCCCGAAACCGCCTACATCATCACCAATCTCATGGAGAGCGTCGTCACGAGCGGCACCGGCCAGCGGGCCC
GGGCCCTGGGCCGCCCCGTGGCGGGAAAGACCGGCACCACCAACGACATGAAGGACGCCTGGTTCGTGGGCTATGTGCCG
CAGCTGGTGGCCGGGGTCTGGGTGGGGTACGATCAGGAGCGCTCCCTTGGCGCCGGAGGGTCGGGCGGGCAGGCCGCGGC
ACCCATCTGGACCGAGTTCATGCAGCGGGCCCTGGCGGGTATCCCCGTGAAGAACTTTATCCAGCCGGCGGACATCACGT
TTGCCACCATCAATGTCCGCACCGGACGCCTGGCCCGGGAAGGGAGTCCGGGGGCGACTCAGGAGTGCTTTGTCGCCGGT
ACCGAGCCCACGGCGTACGATGGCGAGCAGGATACACCCACCACCGAAGAAAATGCCAGCGATTCCGATGGGTTGCCGGA
ATGGGTGCGCGCTGGGCGTCTGCCGCGACGGTCCGAAAGTCGCTAA

Upstream 100 bases:

>100_bases
CCCTGTCCCTGAGATTCAATAACTATCTTGAACTGAAATGCGCATCTGCGCTAAGGTGAAACGTTATCGCCATCGTAGTA
TACCAGATTTGGAGACGCTC

Downstream 100 bases:

>100_bases
TCCCTTGCGGGGCAACGGTTTACGACCCATGAAAAAATTATTTTTCTCCAAATAATCCCTTGCAATGTGGAAATTTCTGT
CTATAGTATTCGCCAGAATT

Product: penicillin-binding protein 1A

Products: NA

Alternate protein names: PBP-1a; PBP1a; Penicillin-insensitive transglycosylase; Peptidoglycan TGase; Penicillin-sensitive transpeptidase; DD-transpeptidase [H]

Number of amino acids: Translated: 841; Mature: 840

Protein sequence:

>841_residues
MTPASGRQNIDPRNVNRRRDKNPWKVVVITLVTLGVVALLAVFGYFTYLYASLPKVDRLADYRPPIVSQVFGDDGSLVGE
FYLERRIVVPVEKIPRKLIQAFVAAEDSNFYQHKGLDYLGIARAAVKNLLAMRKKEGASTITQQVAKSMLLTSEKSFARK
FKEAILATRMEKMLSKDDILYIYLNQIYLGGGAYGVQVAAETYFGKNVEQLNLAEMAMLAGLPKAPNSYSPIKHLDRARE
RQAYVLERMVREGYVTQAEADHARATPIVVRSMKKVNSEQSAYFLEHIRIKLEETYGMDRLYKEGLKIYTTMNAEMQKGA
FDAVVNGLKNVDKRQGFRGAVKYLGETEVDTFSKKVEDGIDTASLKQGSTYQGVVTAVDPAKGDATVRVGDRTGTISRKN
MAWAGKVQLVNAWGKQDGKGKSLPLGSVVFVSVLTPDVNKQGAIFSLDQEPEAQAAVVALDPLTGAVKAMVGGYDFRRSQ
FNRAVQAKRNPGSAFKPIIYAAALDKGLTAATTFDDSPVEYEGGGDKAWKPKNYDNIYRGIVTMREALTNSINVVSVKIL
EQIGVGAAIDYAKKLGITSPLAANLTLALGSSSVTPLELTSAYAVFASGGYRTTPYFITKVEDADGKVLEETAPPVIPTF
TNQSTATLTNADLPPATGAVPGGGAVPAIAPETAYIITNLMESVVTSGTGQRARALGRPVAGKTGTTNDMKDAWFVGYVP
QLVAGVWVGYDQERSLGAGGSGGQAAAPIWTEFMQRALAGIPVKNFIQPADITFATINVRTGRLAREGSPGATQECFVAG
TEPTAYDGEQDTPTTEENASDSDGLPEWVRAGRLPRRSESR

Sequences:

>Translated_841_residues
MTPASGRQNIDPRNVNRRRDKNPWKVVVITLVTLGVVALLAVFGYFTYLYASLPKVDRLADYRPPIVSQVFGDDGSLVGE
FYLERRIVVPVEKIPRKLIQAFVAAEDSNFYQHKGLDYLGIARAAVKNLLAMRKKEGASTITQQVAKSMLLTSEKSFARK
FKEAILATRMEKMLSKDDILYIYLNQIYLGGGAYGVQVAAETYFGKNVEQLNLAEMAMLAGLPKAPNSYSPIKHLDRARE
RQAYVLERMVREGYVTQAEADHARATPIVVRSMKKVNSEQSAYFLEHIRIKLEETYGMDRLYKEGLKIYTTMNAEMQKGA
FDAVVNGLKNVDKRQGFRGAVKYLGETEVDTFSKKVEDGIDTASLKQGSTYQGVVTAVDPAKGDATVRVGDRTGTISRKN
MAWAGKVQLVNAWGKQDGKGKSLPLGSVVFVSVLTPDVNKQGAIFSLDQEPEAQAAVVALDPLTGAVKAMVGGYDFRRSQ
FNRAVQAKRNPGSAFKPIIYAAALDKGLTAATTFDDSPVEYEGGGDKAWKPKNYDNIYRGIVTMREALTNSINVVSVKIL
EQIGVGAAIDYAKKLGITSPLAANLTLALGSSSVTPLELTSAYAVFASGGYRTTPYFITKVEDADGKVLEETAPPVIPTF
TNQSTATLTNADLPPATGAVPGGGAVPAIAPETAYIITNLMESVVTSGTGQRARALGRPVAGKTGTTNDMKDAWFVGYVP
QLVAGVWVGYDQERSLGAGGSGGQAAAPIWTEFMQRALAGIPVKNFIQPADITFATINVRTGRLAREGSPGATQECFVAG
TEPTAYDGEQDTPTTEENASDSDGLPEWVRAGRLPRRSESR
>Mature_840_residues
TPASGRQNIDPRNVNRRRDKNPWKVVVITLVTLGVVALLAVFGYFTYLYASLPKVDRLADYRPPIVSQVFGDDGSLVGEF
YLERRIVVPVEKIPRKLIQAFVAAEDSNFYQHKGLDYLGIARAAVKNLLAMRKKEGASTITQQVAKSMLLTSEKSFARKF
KEAILATRMEKMLSKDDILYIYLNQIYLGGGAYGVQVAAETYFGKNVEQLNLAEMAMLAGLPKAPNSYSPIKHLDRARER
QAYVLERMVREGYVTQAEADHARATPIVVRSMKKVNSEQSAYFLEHIRIKLEETYGMDRLYKEGLKIYTTMNAEMQKGAF
DAVVNGLKNVDKRQGFRGAVKYLGETEVDTFSKKVEDGIDTASLKQGSTYQGVVTAVDPAKGDATVRVGDRTGTISRKNM
AWAGKVQLVNAWGKQDGKGKSLPLGSVVFVSVLTPDVNKQGAIFSLDQEPEAQAAVVALDPLTGAVKAMVGGYDFRRSQF
NRAVQAKRNPGSAFKPIIYAAALDKGLTAATTFDDSPVEYEGGGDKAWKPKNYDNIYRGIVTMREALTNSINVVSVKILE
QIGVGAAIDYAKKLGITSPLAANLTLALGSSSVTPLELTSAYAVFASGGYRTTPYFITKVEDADGKVLEETAPPVIPTFT
NQSTATLTNADLPPATGAVPGGGAVPAIAPETAYIITNLMESVVTSGTGQRARALGRPVAGKTGTTNDMKDAWFVGYVPQ
LVAGVWVGYDQERSLGAGGSGGQAAAPIWTEFMQRALAGIPVKNFIQPADITFATINVRTGRLAREGSPGATQECFVAGT
EPTAYDGEQDTPTTEENASDSDGLPEWVRAGRLPRRSESR

Specific function: Cell wall formation. Synthesis of cross-linked peptidoglycan from the lipid intermediates. The enzyme has a penicillin-insensitive transglycosylase N-terminal domain (formation of linear glycan strands) and a penicillin-sensitive transpeptidase C-terminal

COG id: COG5009

COG function: function code M; Membrane carboxypeptidase/penicillin-binding protein

Gene ontology:

Cell location: Cell inner membrane; Single-pass type II membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI87082258, Length=839, Percent_Identity=36.4719904648391, Blast_Score=479, Evalue=1e-136,
Organism=Escherichia coli, GI1786343, Length=256, Percent_Identity=35.9375, Blast_Score=145, Evalue=9e-36,
Organism=Escherichia coli, GI1788867, Length=193, Percent_Identity=34.7150259067358, Blast_Score=116, Evalue=5e-27,
Organism=Escherichia coli, GI1789601, Length=189, Percent_Identity=34.9206349206349, Blast_Score=99, Evalue=9e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001264
- InterPro:   IPR011816
- InterPro:   IPR001460 [H]

Pfam domain/function: PF00912 Transgly; PF00905 Transpeptidase [H]

EC number: 2.4.2.-; 3.4.-.-

Molecular weight: Translated: 91044; Mature: 90912

Theoretical pI: Translated: 9.49; Mature: 9.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPASGRQNIDPRNVNRRRDKNPWKVVVITLVTLGVVALLAVFGYFTYLYASLPKVDRLA
CCCCCCCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
DYRPPIVSQVFGDDGSLVGEFYLERRIVVPVEKIPRKLIQAFVAAEDSNFYQHKGLDYLG
CCCCCHHHHHHCCCCCHHHHHHHCCEEECCHHHHHHHHHHHHHHCCCCCCHHHCCCCHHH
IARAAVKNLLAMRKKEGASTITQQVAKSMLLTSEKSFARKFKEAILATRMEKMLSKDDIL
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCEE
YIYLNQIYLGGGAYGVQVAAETYFGKNVEQLNLAEMAMLAGLPKAPNSYSPIKHLDRARE
EEEEEEEEECCCCCEEEEEEHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
RQAYVLERMVREGYVTQAEADHARATPIVVRSMKKVNSEQSAYFLEHIRIKLEETYGMDR
HHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCHHH
LYKEGLKIYTTMNAEMQKGAFDAVVNGLKNVDKRQGFRGAVKYLGETEVDTFSKKVEDGI
HHHCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCHHHHHHHHHHHCC
DTASLKQGSTYQGVVTAVDPAKGDATVRVGDRTGTISRKNMAWAGKVQLVNAWGKQDGKG
CCHHCCCCCCCCCEEEEECCCCCCEEEEECCCCCCCCCCCCCEECCEEEEECCCCCCCCC
KSLPLGSVVFVSVLTPDVNKQGAIFSLDQEPEAQAAVVALDPLTGAVKAMVGGYDFRRSQ
CCCCCCCEEEEEEECCCCCCCCCEEEECCCCCCCEEEEEECCHHHHHHHHHCCCHHHHHH
FNRAVQAKRNPGSAFKPIIYAAALDKGLTAATTFDDSPVEYEGGGDKAWKPKNYDNIYRG
HHHHHHHCCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCEECCCCCCCCCCCCHHHHHHH
IVTMREALTNSINVVSVKILEQIGVGAAIDYAKKLGITSPLAANLTLALGSSSVTPLELT
HHHHHHHHHCCCCEEHHHHHHHHCCCHHHHHHHHCCCCCCCCCCEEEEECCCCCCCEEHH
SAYAVFASGGYRTTPYFITKVEDADGKVLEETAPPVIPTFTNQSTATLTNADLPPATGAV
HHEEEEECCCCEECCEEEEEEECCCCCEEHHCCCCCCCCCCCCCCEEEECCCCCCCCCCC
PGGGAVPAIAPETAYIITNLMESVVTSGTGQRARALGRPVAGKTGTTNDMKDAWFVGYVP
CCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCCCCCCCCCCHHHHHHHH
QLVAGVWVGYDQERSLGAGGSGGQAAAPIWTEFMQRALAGIPVKNFIQPADITFATINVR
HHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHCCCCCCEEEEEEEEE
TGRLAREGSPGATQECFVAGTEPTAYDGEQDTPTTEENASDSDGLPEWVRAGRLPRRSES
CCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCC
R
C
>Mature Secondary Structure 
TPASGRQNIDPRNVNRRRDKNPWKVVVITLVTLGVVALLAVFGYFTYLYASLPKVDRLA
CCCCCCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
DYRPPIVSQVFGDDGSLVGEFYLERRIVVPVEKIPRKLIQAFVAAEDSNFYQHKGLDYLG
CCCCCHHHHHHCCCCCHHHHHHHCCEEECCHHHHHHHHHHHHHHCCCCCCHHHCCCCHHH
IARAAVKNLLAMRKKEGASTITQQVAKSMLLTSEKSFARKFKEAILATRMEKMLSKDDIL
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCEE
YIYLNQIYLGGGAYGVQVAAETYFGKNVEQLNLAEMAMLAGLPKAPNSYSPIKHLDRARE
EEEEEEEEECCCCCEEEEEEHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
RQAYVLERMVREGYVTQAEADHARATPIVVRSMKKVNSEQSAYFLEHIRIKLEETYGMDR
HHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCHHH
LYKEGLKIYTTMNAEMQKGAFDAVVNGLKNVDKRQGFRGAVKYLGETEVDTFSKKVEDGI
HHHCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCHHHHHHHHHHHCC
DTASLKQGSTYQGVVTAVDPAKGDATVRVGDRTGTISRKNMAWAGKVQLVNAWGKQDGKG
CCHHCCCCCCCCCEEEEECCCCCCEEEEECCCCCCCCCCCCCEECCEEEEECCCCCCCCC
KSLPLGSVVFVSVLTPDVNKQGAIFSLDQEPEAQAAVVALDPLTGAVKAMVGGYDFRRSQ
CCCCCCCEEEEEEECCCCCCCCCEEEECCCCCCCEEEEEECCHHHHHHHHHCCCHHHHHH
FNRAVQAKRNPGSAFKPIIYAAALDKGLTAATTFDDSPVEYEGGGDKAWKPKNYDNIYRG
HHHHHHHCCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCEECCCCCCCCCCCCHHHHHHH
IVTMREALTNSINVVSVKILEQIGVGAAIDYAKKLGITSPLAANLTLALGSSSVTPLELT
HHHHHHHHHCCCCEEHHHHHHHHCCCHHHHHHHHCCCCCCCCCCEEEEECCCCCCCEEHH
SAYAVFASGGYRTTPYFITKVEDADGKVLEETAPPVIPTFTNQSTATLTNADLPPATGAV
HHEEEEECCCCEECCEEEEEEECCCCCEEHHCCCCCCCCCCCCCCEEEECCCCCCCCCCC
PGGGAVPAIAPETAYIITNLMESVVTSGTGQRARALGRPVAGKTGTTNDMKDAWFVGYVP
CCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCCCCCCCCCCHHHHHHHH
QLVAGVWVGYDQERSLGAGGSGGQAAAPIWTEFMQRALAGIPVKNFIQPADITFATINVR
HHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHCCCCCCEEEEEEEEE
TGRLAREGSPGATQECFVAGTEPTAYDGEQDTPTTEENASDSDGLPEWVRAGRLPRRSES
CCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCC
R
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Acyltransferases; Aminoacyltransferases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA