Click here to switch to the map view.

The map label for this gene is narI [H]

Identifier: 78221553

GI number: 78221553

Start: 371063

End: 371758

Strand: Direct

Name: narI [H]

Synonym: Gmet_0332

Alternate gene names: 78221553

Gene position: 371063-371758 (Clockwise)

Preceding gene: 78221552

Following gene: 78221554

Centisome position: 9.28

GC content: 61.64

Gene sequence:

>696_bases
ATGCTGAACACCGTGCTGAACACCTTTATCTTTGTGGCCCTTCCCTATGTGGCCCTGGCGATGCTCATCGTCGTCACCCC
CTACCGCTATTACTCGAACCGTCTCACCTGGTCGGCCTACTCCACCCAGTTCCTGGAGCGGAAGACCCTCTACTGGGGGG
CCAACCCTTGGCACTACGGGATCATCCCCATCATACTGGCCCACACCCTTGGCGTTGTGGCCCCCGGTGCCATGAAGTCG
CTCTTGGGGAACGTTGAGGTCCTGGTCTTTCTGGAGAGCGTGGGGCTCGGCCTGGGGCTCTTCGCGCTCTTCGGCTGCGT
GGTGCTCCTGCTGCGTCGCGTCAACGCTCCCATGCTGAAGCGCGTAACCTATCCCGCCGACTGGGTGCTCCTCATTCTGC
TGACCTTCCAGACGCTCACCGGGGTCTACATCAGCCTCTTCATGCGCTGGGGCTCCCAGTGGTACCTCCACACGGCGGTT
CCCTACCTCTATTCGCTCCTCTACTTCAACCCGAAGGTGGAGTACCTGGCCGACTTCCCCCTCATCCTCAAGCTGCACGC
GGCAGGGGCATTCCTGATCGTGGCGCTCCTGCCGTTCACCAAGCTGGTCCACCTCCTCTACTGGCCTCTCGATTTTCTCA
AGGATCCACCCATCCTCTACCGCTGGCGCTCCCGGCCCGAGGGGAAGGTGGAGTAA

Upstream 100 bases:

>100_bases
GCGAGGACTCCGCCCGGGGAGAGCACCCCGCGGAAGGGATCGGCGATGGCGTTCTTGAAAAAAACCGCCCGAAAACGGCT
GCGCATCCGGAGGTAAGCCC

Downstream 100 bases:

>100_bases
CGGCATTCAAGGAGAACGCCATGGAAAGGCACGAGCAGAAGACGGCAACGGCGGCAACGTGGGGGACCCAGGTCAGGACG
CGCACCATGGCGACCCACAG

Product: respiratory nitrate reductase subunit gamma

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 231; Mature: 231

Protein sequence:

>231_residues
MLNTVLNTFIFVALPYVALAMLIVVTPYRYYSNRLTWSAYSTQFLERKTLYWGANPWHYGIIPIILAHTLGVVAPGAMKS
LLGNVEVLVFLESVGLGLGLFALFGCVVLLLRRVNAPMLKRVTYPADWVLLILLTFQTLTGVYISLFMRWGSQWYLHTAV
PYLYSLLYFNPKVEYLADFPLILKLHAAGAFLIVALLPFTKLVHLLYWPLDFLKDPPILYRWRSRPEGKVE

Sequences:

>Translated_231_residues
MLNTVLNTFIFVALPYVALAMLIVVTPYRYYSNRLTWSAYSTQFLERKTLYWGANPWHYGIIPIILAHTLGVVAPGAMKS
LLGNVEVLVFLESVGLGLGLFALFGCVVLLLRRVNAPMLKRVTYPADWVLLILLTFQTLTGVYISLFMRWGSQWYLHTAV
PYLYSLLYFNPKVEYLADFPLILKLHAAGAFLIVALLPFTKLVHLLYWPLDFLKDPPILYRWRSRPEGKVE
>Mature_231_residues
MLNTVLNTFIFVALPYVALAMLIVVTPYRYYSNRLTWSAYSTQFLERKTLYWGANPWHYGIIPIILAHTLGVVAPGAMKS
LLGNVEVLVFLESVGLGLGLFALFGCVVLLLRRVNAPMLKRVTYPADWVLLILLTFQTLTGVYISLFMRWGSQWYLHTAV
PYLYSLLYFNPKVEYLADFPLILKLHAAGAFLIVALLPFTKLVHLLYWPLDFLKDPPILYRWRSRPEGKVE

Specific function: The Nitrate Reductase Enzyme Complex Allows E.Coli To Use Nitrate As An Electron Acceptor During Anaerobic Growth. The Gamma Chain Is A Membrane-Embedded Heme-Iron Unit Resembling Cytochrome B, Which Transfers Electrons From Quinones To The Beta Subunit.

COG id: COG2181

COG function: function code C; Nitrate reductase gamma subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787480, Length=223, Percent_Identity=30.0448430493274, Blast_Score=84, Evalue=5e-18,
Organism=Escherichia coli, GI1787738, Length=221, Percent_Identity=25.3393665158371, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003816 [H]

Pfam domain/function: PF02665 Nitrate_red_gam [H]

EC number: =1.7.99.4 [H]

Molecular weight: Translated: 26505; Mature: 26505

Theoretical pI: Translated: 9.80; Mature: 9.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNTVLNTFIFVALPYVALAMLIVVTPYRYYSNRLTWSAYSTQFLERKTLYWGANPWHYG
CHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCEEEHHHHHHHHHHHHEEECCCCCCHH
IIPIILAHTLGVVAPGAMKSLLGNVEVLVFLESVGLGLGLFALFGCVVLLLRRVNAPMLK
HHHHHHHHHHHHCCCHHHHHHHCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCHHHH
RVTYPADWVLLILLTFQTLTGVYISLFMRWGSQWYLHTAVPYLYSLLYFNPKVEYLADFP
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHCCH
LILKLHAAGAFLIVALLPFTKLVHLLYWPLDFLKDPPILYRWRSRPEGKVE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCC
>Mature Secondary Structure
MLNTVLNTFIFVALPYVALAMLIVVTPYRYYSNRLTWSAYSTQFLERKTLYWGANPWHYG
CHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCEEEHHHHHHHHHHHHEEECCCCCCHH
IIPIILAHTLGVVAPGAMKSLLGNVEVLVFLESVGLGLGLFALFGCVVLLLRRVNAPMLK
HHHHHHHHHHHHCCCHHHHHHHCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCHHHH
RVTYPADWVLLILLTFQTLTGVYISLFMRWGSQWYLHTAVPYLYSLLYFNPKVEYLADFP
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHCCH
LILKLHAAGAFLIVALLPFTKLVHLLYWPLDFLKDPPILYRWRSRPEGKVE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8846791; 9353933; 9384377; 7557333 [H]