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The map label for this gene is nrfA [H]

Identifier: 78221517

GI number: 78221517

Start: 330005

End: 331471

Strand: Reverse

Name: nrfA [H]

Synonym: Gmet_0294

Alternate gene names: 78221517

Gene position: 331471-330005 (Counterclockwise)

Preceding gene: 78221518

Following gene: 78221516

Centisome position: 8.29

GC content: 61.9

Gene sequence:

>1467_bases
ATGAGACGACTGAATCCTGTTGCACCCCTGATCGCGCTGGCTGCCGCGGCAACCCTGTTTGTCCTCGGGGGATGCGCCCC
GGAGAAAGCCCAGCCGGTAAAGACGGTCCAGATCCCCGATGGCGAAGTCGACCCGGCCGTCTGGGGCAAGGCCTATCCGA
CCGAATACGAAATGTGGAAAAAGACCGAGGAGCCGGAGCCTCCTGGCAAGAGCAAGTACAAGCGGGGCTTCGACGCCGAC
CACGTCACCTACGACAAGCTCTCCGAGTTCCCCTACATGGCACTCCTCTTCAACGGCTGGGGGTTCGGCATCGCCTACAA
CGAGCCCCGGGGCCACGCCAACATGGTGCGAGACCAGTTGGAGATCGACTCGGCCCGCCTCAAGTCGGGGGGCGTCTGCC
TCACCTGCAAGACCCCCTACGCGCCCAAACTCGAAAAGGAGATGGGGATCGACTACTTCAAGACCCCCTTCAAGGATGTC
CTCGCCAAGATTCCCGAAAAGCATAAGACCCTCGGGGTGGCCTGCATCGACTGCCACGACAACAAGGACATGAGTCTCAG
GATTTCCCGAGGCTTCACCCTCGGAGAGGCCCTGAAGAAGCTGGGGGTCGACCAGGCGAAGCTGTCCCGCCAGGAGATGC
GGAGCCTCGTCTGCGCCCAGTGCCACGTCACCTACAATATTCCCAAGGACGCCGACAAGAAGTCGATCGGAGTCTACTTC
CCCTGGCAGGGAAGCAAGATGGGCAACATCTCCGTCGAGAACATCATCAAGCAGATCCGCAGCGATGCCAGCGTGGGGGA
GTGGACGCAGACAGTCACCGGCTTCAAGCTGGGATTCATCCGCCACCCCGAGTATGAGCTCTTCTCCAACAACAGCGTCC
ACTGGAAGGCCGGGGCGGCCTGCACCGACTGTCATATGCCCTACACCCGGGTCGGCGCCTTCAAGGTATCGGACCACCGG
GTCATGAGTCCCCTGAAGAATGACATGAAGGCCTGCATCCAATGCCACACCGAAAAACCCGAATGGCTCCGGGATCAGGT
CATCGCCATCCAGGACCGGACCGTCTCCCTCATGCTCCGCTCCGGCTACGCCACCGCAACCGTGGCCAAACTCTTCGAGA
AGGCCCATGCCGCCCAGGCCCAGGGAAAACAGATCGACAAGGCCCTCTACGACCGGGCGAAAGATCTCTATGAGGAAGCC
TTCTACCGCTGCGTCTTCATCGGCGCCGAAAACTCCGTCGGCTTCCACAACCCCACCGAGGCCATGCGGGTGCTGGGGGA
CGCCACCGCCTTCGCCACCAAGGCCGAGGCCCTGCTCCGTCAGGCCCTGGCCAAGGCCGGCGTCGATGTGCCGCTCACCG
TCAACCTGGAGCTGAACAAGTACCTGGATCAACGGGGCGAGAAGAAGCTGACGTTTGACCCGAAGGTGGAGATCAAGGAC
CCCTACGGCGTGCAGGTCCGGTTCTGA

Upstream 100 bases:

>100_bases
CCGTGACCCACATCGATACCGAACGCCAATGCTGGAAATGCCACCGCCGCGTCTCCCACACGCGGAGCGGGGCCATGGAA
ACCTTATAGGAGGCTCAACA

Downstream 100 bases:

>100_bases
TCCGCTGCTGACGCCGATCATGGGCTAAGAAAGCACAGGCCGTCCGGGTGTTCCGGGCGGCCTTTGTGTTACCTGATGTT
TTCCGCCTAAAGGTCCTGTT

Product: respiratory nitrite reductase (cytochrome; ammonia-forming) precursor

Products: NA

Alternate protein names: Ammonia-forming cytochrome c nitrite reductase; Cytochrome c nitrite reductase [H]

Number of amino acids: Translated: 488; Mature: 488

Protein sequence:

>488_residues
MRRLNPVAPLIALAAAATLFVLGGCAPEKAQPVKTVQIPDGEVDPAVWGKAYPTEYEMWKKTEEPEPPGKSKYKRGFDAD
HVTYDKLSEFPYMALLFNGWGFGIAYNEPRGHANMVRDQLEIDSARLKSGGVCLTCKTPYAPKLEKEMGIDYFKTPFKDV
LAKIPEKHKTLGVACIDCHDNKDMSLRISRGFTLGEALKKLGVDQAKLSRQEMRSLVCAQCHVTYNIPKDADKKSIGVYF
PWQGSKMGNISVENIIKQIRSDASVGEWTQTVTGFKLGFIRHPEYELFSNNSVHWKAGAACTDCHMPYTRVGAFKVSDHR
VMSPLKNDMKACIQCHTEKPEWLRDQVIAIQDRTVSLMLRSGYATATVAKLFEKAHAAQAQGKQIDKALYDRAKDLYEEA
FYRCVFIGAENSVGFHNPTEAMRVLGDATAFATKAEALLRQALAKAGVDVPLTVNLELNKYLDQRGEKKLTFDPKVEIKD
PYGVQVRF

Sequences:

>Translated_488_residues
MRRLNPVAPLIALAAAATLFVLGGCAPEKAQPVKTVQIPDGEVDPAVWGKAYPTEYEMWKKTEEPEPPGKSKYKRGFDAD
HVTYDKLSEFPYMALLFNGWGFGIAYNEPRGHANMVRDQLEIDSARLKSGGVCLTCKTPYAPKLEKEMGIDYFKTPFKDV
LAKIPEKHKTLGVACIDCHDNKDMSLRISRGFTLGEALKKLGVDQAKLSRQEMRSLVCAQCHVTYNIPKDADKKSIGVYF
PWQGSKMGNISVENIIKQIRSDASVGEWTQTVTGFKLGFIRHPEYELFSNNSVHWKAGAACTDCHMPYTRVGAFKVSDHR
VMSPLKNDMKACIQCHTEKPEWLRDQVIAIQDRTVSLMLRSGYATATVAKLFEKAHAAQAQGKQIDKALYDRAKDLYEEA
FYRCVFIGAENSVGFHNPTEAMRVLGDATAFATKAEALLRQALAKAGVDVPLTVNLELNKYLDQRGEKKLTFDPKVEIKD
PYGVQVRF
>Mature_488_residues
MRRLNPVAPLIALAAAATLFVLGGCAPEKAQPVKTVQIPDGEVDPAVWGKAYPTEYEMWKKTEEPEPPGKSKYKRGFDAD
HVTYDKLSEFPYMALLFNGWGFGIAYNEPRGHANMVRDQLEIDSARLKSGGVCLTCKTPYAPKLEKEMGIDYFKTPFKDV
LAKIPEKHKTLGVACIDCHDNKDMSLRISRGFTLGEALKKLGVDQAKLSRQEMRSLVCAQCHVTYNIPKDADKKSIGVYF
PWQGSKMGNISVENIIKQIRSDASVGEWTQTVTGFKLGFIRHPEYELFSNNSVHWKAGAACTDCHMPYTRVGAFKVSDHR
VMSPLKNDMKACIQCHTEKPEWLRDQVIAIQDRTVSLMLRSGYATATVAKLFEKAHAAQAQGKQIDKALYDRAKDLYEEA
FYRCVFIGAENSVGFHNPTEAMRVLGDATAFATKAEALLRQALAKAGVDVPLTVNLELNKYLDQRGEKKLTFDPKVEIKD
PYGVQVRF

Specific function: Plays a role in nitrite reduction [H]

COG id: COG3303

COG function: function code P; Formate-dependent nitrite reductase, periplasmic cytochrome c552 subunit

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the cytochrome c-552 family [H]

Homologues:

Organism=Escherichia coli, GI1790506, Length=457, Percent_Identity=27.7899343544858, Blast_Score=141, Evalue=8e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003321
- InterPro:   IPR017570
- InterPro:   IPR011031 [H]

Pfam domain/function: PF02335 Cytochrom_C552 [H]

EC number: =1.7.2.2 [H]

Molecular weight: Translated: 54587; Mature: 54587

Theoretical pI: Translated: 8.71; Mature: 8.71

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS51008 MULTIHEME_CYTC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRLNPVAPLIALAAAATLFVLGGCAPEKAQPVKTVQIPDGEVDPAVWGKAYPTEYEMWK
CCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCHHHCCCCCCCHHHHHH
KTEEPEPPGKSKYKRGFDADHVTYDKLSEFPYMALLFNGWGFGIAYNEPRGHANMVRDQL
CCCCCCCCCHHHHHCCCCCCCCCHHHHHCCCEEEEEEECCCEEEEECCCCCHHHHHHHHH
EIDSARLKSGGVCLTCKTPYAPKLEKEMGIDYFKTPFKDVLAKIPEKHKTLGVACIDCHD
HHHHHHCCCCCEEEEECCCCCCCHHHHHCCCHHHCCHHHHHHHCCHHHHEEEEEEEEECC
NKDMSLRISRGFTLGEALKKLGVDQAKLSRQEMRSLVCAQCHVTYNIPKDADKKSIGVYF
CCCCEEEEECCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCEEEEE
PWQGSKMGNISVENIIKQIRSDASVGEWTQTVTGFKLGFIRHPEYELFSNNSVHWKAGAA
CCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHCCEEEEEEECCCHHEECCCCEEEECCCE
CTDCHMPYTRVGAFKVSDHRVMSPLKNDMKACIQCHTEKPEWLRDQVIAIQDRTVSLMLR
EEECCCCHHHCCEEEECCHHHHHHHHHHHHHHHHHCCCCCHHHHHCEEEEECCEEEEEEE
SGYATATVAKLFEKAHAAQAQGKQIDKALYDRAKDLYEEAFYRCVFIGAENSVGFHNPTE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCCCCCHHH
AMRVLGDATAFATKAEALLRQALAKAGVDVPLTVNLELNKYLDQRGEKKLTFDPKVEIKD
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEHHHHHHCCCCCCEECCCCEEECC
PYGVQVRF
CCCEEEEC
>Mature Secondary Structure
MRRLNPVAPLIALAAAATLFVLGGCAPEKAQPVKTVQIPDGEVDPAVWGKAYPTEYEMWK
CCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCHHHCCCCCCCHHHHHH
KTEEPEPPGKSKYKRGFDADHVTYDKLSEFPYMALLFNGWGFGIAYNEPRGHANMVRDQL
CCCCCCCCCHHHHHCCCCCCCCCHHHHHCCCEEEEEEECCCEEEEECCCCCHHHHHHHHH
EIDSARLKSGGVCLTCKTPYAPKLEKEMGIDYFKTPFKDVLAKIPEKHKTLGVACIDCHD
HHHHHHCCCCCEEEEECCCCCCCHHHHHCCCHHHCCHHHHHHHCCHHHHEEEEEEEEECC
NKDMSLRISRGFTLGEALKKLGVDQAKLSRQEMRSLVCAQCHVTYNIPKDADKKSIGVYF
CCCCEEEEECCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCEEEEE
PWQGSKMGNISVENIIKQIRSDASVGEWTQTVTGFKLGFIRHPEYELFSNNSVHWKAGAA
CCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHCCEEEEEEECCCHHEECCCCEEEECCCE
CTDCHMPYTRVGAFKVSDHRVMSPLKNDMKACIQCHTEKPEWLRDQVIAIQDRTVSLMLR
EEECCCCHHHCCEEEECCHHHHHHHHHHHHHHHHHCCCCCHHHHHCEEEEECCEEEEEEE
SGYATATVAKLFEKAHAAQAQGKQIDKALYDRAKDLYEEAFYRCVFIGAENSVGFHNPTE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCCCCCHHH
AMRVLGDATAFATKAEALLRQALAKAGVDVPLTVNLELNKYLDQRGEKKLTFDPKVEIKD
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEHHHHHHCCCCCCEECCCCEEECC
PYGVQVRF
CCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA