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The map label for this gene is prfC

Identifier: 78221416

GI number: 78221416

Start: 222076

End: 223659

Strand: Direct

Name: prfC

Synonym: Gmet_0192

Alternate gene names: 78221416

Gene position: 222076-223659 (Clockwise)

Preceding gene: 78221414

Following gene: 78221417

Centisome position: 5.56

GC content: 64.71

Gene sequence:

>1584_bases
TTGACGAAGCACAACGAGCAGGAAATCGACCGCCGGCGAACCTTCGCCATCATCAGCCACCCGGACGCCGGCAAGACCAC
CATCACCGAGAAGCTGCTGCTGTTCGGCGGCGCCATCCAGCAGGCCGGCGAGGTTCGCGCCCGCAAGGCGGCCCGCCATG
CCACCTCCGACTGGATGGAGATGGAGAAGCAGCGGGGGATCTCGGTCACCTCGTCGGTCATGAAGTTCACCTACCGGAAC
TACGAGGTGAACCTCCTGGACACCCCGGGCCACAACGATTTTTCCGAGGACACCTACCGGGTCCTCACCGCCGTGGACTC
GGCCCTCATGGTCATCGACGCGGTCAAGGGGGTCGAGAGCCAGACCATCAAGCTCCTCGATGTCTGCCGCCTCCGCCACA
CCCCCATCATGACCTTCGTCAACAAGCTGGACCGTGAAGGGCGCGATCCCTTCGAGCTCATCGACGAGATTGAGAAGGTC
CTGAAGATCCAGTGCGCGCCCATGACCTGGCCCATCGGCATGGGGAAGCGGTTCCGGGGGACCTACCACCTCTACACCAA
GGAGCTCATCATCTTCGACGCCGAGGCGGAGCGGGGGACCGGGAGCATCGTGTCGCTCTCCGGCCTCGACGATCCCCGCC
TCGACGAGATTCTCGGCTCCCAGGCCGCGGAGCTTCGCGGTGACATCGAGCTTCTGGAGGGGGCGGCCCACCCCTTCGAG
GAGGAGGCGTATCTCGCCGGCCTCCAGACCCCGGTCTTCTTCGGGAGCGCCATCAACACCTTCGGGGTGCAGCAACTCCT
CGACACCTTCGTGGAGAACGCCCCGGCGCCGCTTCCCCGGGAGGCAGTCACCCGTACCGTCTCTCCCTACGAGGAGCCCT
TTGCCGCCTTCGCCTTCAAGATCCAGGCGAACATGGACCCGGCCCACCGGGACCGGATCGCCTTCTTCCGCATCTGCTCC
GGCAAGTTCACCCGGGGGATGAAGGTGCGCCACGTGCGGCTCGGCCGCGAGGTGGCCATCAACAACGCCACCATCTTCAT
GGCCCAGGACCGGACCCACGTGGACGAGGCGTTCCCCGGCGACATCATCGGCATCCATAACCACGGCACCATCAAGATCG
GCGACACCTTCACCATGGGTGAGGAGCTGAAGTTCACCGGCATCCCCAACTTCGCCCCGGAGCACTTCCGCAAGGTGCGG
CTCCTGGACCCGCTCAAATCCAAGGCCCTGGAGAAGGGGCTCACGCAGTTGGCCGAGGAGGGGACCACCCAGGTCTTTCG
GCCGCTGATGGGGGCCGACTGGATCGTCGGGGCCGTGGGTATCCTCCAGTTCGACGTGGTCATGCACCGGTTGGAGCACG
AGTACAACGTGAAGGCCACCTACGAGCCCGCCGCCTACGCCACGGCCCGGTGGGTGACCGGCGAGAAGAAGAAGCTGGAG
GAGTTCCAGAAGAAAGAGGTCATGAGCTGCTACATCGACGGTGAGGGGAACCTGGCCTACCTGGCCAGTTCCCAGTGGCG
CCTCGACAACACCATGGACAACTGGAAGGATCTCCAGTTCCACGCCACGCGGGAGCATAGCTGA

Upstream 100 bases:

>100_bases
CGCGGCGATTTCGTGTATAGTCAATTCTGGCGAAGATATCCTCTTCGCCTTTCGTTTGTCCAGAAATTTCAGCACTTTTC
ACCATGACGGAGAGCAGACT

Downstream 100 bases:

>100_bases
GCCGTTAAGAAAGGGGGGAGACACTGGGTTTCCCCCCTTTTGTCTTCATCACGCCAAACCCACTGGCCCCTTTCCCGGCA
GCACGCATTTTCAACCCCTG

Product: peptide chain release factor 3

Products: NA

Alternate protein names: RF-3

Number of amino acids: Translated: 527; Mature: 526

Protein sequence:

>527_residues
MTKHNEQEIDRRRTFAIISHPDAGKTTITEKLLLFGGAIQQAGEVRARKAARHATSDWMEMEKQRGISVTSSVMKFTYRN
YEVNLLDTPGHNDFSEDTYRVLTAVDSALMVIDAVKGVESQTIKLLDVCRLRHTPIMTFVNKLDREGRDPFELIDEIEKV
LKIQCAPMTWPIGMGKRFRGTYHLYTKELIIFDAEAERGTGSIVSLSGLDDPRLDEILGSQAAELRGDIELLEGAAHPFE
EEAYLAGLQTPVFFGSAINTFGVQQLLDTFVENAPAPLPREAVTRTVSPYEEPFAAFAFKIQANMDPAHRDRIAFFRICS
GKFTRGMKVRHVRLGREVAINNATIFMAQDRTHVDEAFPGDIIGIHNHGTIKIGDTFTMGEELKFTGIPNFAPEHFRKVR
LLDPLKSKALEKGLTQLAEEGTTQVFRPLMGADWIVGAVGILQFDVVMHRLEHEYNVKATYEPAAYATARWVTGEKKKLE
EFQKKEVMSCYIDGEGNLAYLASSQWRLDNTMDNWKDLQFHATREHS

Sequences:

>Translated_527_residues
MTKHNEQEIDRRRTFAIISHPDAGKTTITEKLLLFGGAIQQAGEVRARKAARHATSDWMEMEKQRGISVTSSVMKFTYRN
YEVNLLDTPGHNDFSEDTYRVLTAVDSALMVIDAVKGVESQTIKLLDVCRLRHTPIMTFVNKLDREGRDPFELIDEIEKV
LKIQCAPMTWPIGMGKRFRGTYHLYTKELIIFDAEAERGTGSIVSLSGLDDPRLDEILGSQAAELRGDIELLEGAAHPFE
EEAYLAGLQTPVFFGSAINTFGVQQLLDTFVENAPAPLPREAVTRTVSPYEEPFAAFAFKIQANMDPAHRDRIAFFRICS
GKFTRGMKVRHVRLGREVAINNATIFMAQDRTHVDEAFPGDIIGIHNHGTIKIGDTFTMGEELKFTGIPNFAPEHFRKVR
LLDPLKSKALEKGLTQLAEEGTTQVFRPLMGADWIVGAVGILQFDVVMHRLEHEYNVKATYEPAAYATARWVTGEKKKLE
EFQKKEVMSCYIDGEGNLAYLASSQWRLDNTMDNWKDLQFHATREHS
>Mature_526_residues
TKHNEQEIDRRRTFAIISHPDAGKTTITEKLLLFGGAIQQAGEVRARKAARHATSDWMEMEKQRGISVTSSVMKFTYRNY
EVNLLDTPGHNDFSEDTYRVLTAVDSALMVIDAVKGVESQTIKLLDVCRLRHTPIMTFVNKLDREGRDPFELIDEIEKVL
KIQCAPMTWPIGMGKRFRGTYHLYTKELIIFDAEAERGTGSIVSLSGLDDPRLDEILGSQAAELRGDIELLEGAAHPFEE
EAYLAGLQTPVFFGSAINTFGVQQLLDTFVENAPAPLPREAVTRTVSPYEEPFAAFAFKIQANMDPAHRDRIAFFRICSG
KFTRGMKVRHVRLGREVAINNATIFMAQDRTHVDEAFPGDIIGIHNHGTIKIGDTFTMGEELKFTGIPNFAPEHFRKVRL
LDPLKSKALEKGLTQLAEEGTTQVFRPLMGADWIVGAVGILQFDVVMHRLEHEYNVKATYEPAAYATARWVTGEKKKLEE
FQKKEVMSCYIDGEGNLAYLASSQWRLDNTMDNWKDLQFHATREHS

Specific function: Increases the formation of ribosomal termination complexes and stimulates activities of RF-1 and RF-2. It binds guanine nucleotides and has strong preference for UGA stop codons. It may interact directly with the ribosome. The stimulation of RF- 1 and RF-

COG id: COG4108

COG function: function code J; Peptide chain release factor RF-3

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. PrfC subfamily

Homologues:

Organism=Homo sapiens, GI18390331, Length=494, Percent_Identity=28.1376518218623, Blast_Score=167, Evalue=2e-41,
Organism=Homo sapiens, GI19923640, Length=510, Percent_Identity=25.6862745098039, Blast_Score=142, Evalue=1e-33,
Organism=Homo sapiens, GI25306283, Length=394, Percent_Identity=27.6649746192893, Blast_Score=139, Evalue=8e-33,
Organism=Homo sapiens, GI25306287, Length=310, Percent_Identity=29.6774193548387, Blast_Score=129, Evalue=7e-30,
Organism=Homo sapiens, GI157426893, Length=172, Percent_Identity=30.2325581395349, Blast_Score=96, Evalue=7e-20,
Organism=Homo sapiens, GI94966754, Length=137, Percent_Identity=33.5766423357664, Blast_Score=79, Evalue=1e-14,
Organism=Homo sapiens, GI4503483, Length=149, Percent_Identity=29.5302013422819, Blast_Score=71, Evalue=3e-12,
Organism=Homo sapiens, GI310132016, Length=115, Percent_Identity=35.6521739130435, Blast_Score=70, Evalue=6e-12,
Organism=Homo sapiens, GI310110807, Length=115, Percent_Identity=35.6521739130435, Blast_Score=70, Evalue=6e-12,
Organism=Homo sapiens, GI310123363, Length=115, Percent_Identity=35.6521739130435, Blast_Score=70, Evalue=6e-12,
Organism=Escherichia coli, GI1790835, Length=522, Percent_Identity=60.727969348659, Blast_Score=661, Evalue=0.0,
Organism=Escherichia coli, GI1789738, Length=511, Percent_Identity=29.1585127201566, Blast_Score=161, Evalue=8e-41,
Organism=Escherichia coli, GI48994988, Length=149, Percent_Identity=34.8993288590604, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1788922, Length=156, Percent_Identity=32.6923076923077, Blast_Score=94, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17533571, Length=501, Percent_Identity=27.3453093812375, Blast_Score=175, Evalue=6e-44,
Organism=Caenorhabditis elegans, GI17556745, Length=166, Percent_Identity=32.5301204819277, Blast_Score=108, Evalue=8e-24,
Organism=Caenorhabditis elegans, GI17557151, Length=153, Percent_Identity=33.3333333333333, Blast_Score=92, Evalue=8e-19,
Organism=Caenorhabditis elegans, GI71988819, Length=133, Percent_Identity=31.5789473684211, Blast_Score=72, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI71988811, Length=133, Percent_Identity=31.5789473684211, Blast_Score=72, Evalue=8e-13,
Organism=Caenorhabditis elegans, GI17506493, Length=168, Percent_Identity=28.5714285714286, Blast_Score=71, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6323098, Length=479, Percent_Identity=27.5574112734864, Blast_Score=175, Evalue=2e-44,
Organism=Saccharomyces cerevisiae, GI6322359, Length=416, Percent_Identity=27.8846153846154, Blast_Score=126, Evalue=7e-30,
Organism=Saccharomyces cerevisiae, GI6323320, Length=155, Percent_Identity=27.0967741935484, Blast_Score=76, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6324707, Length=149, Percent_Identity=30.8724832214765, Blast_Score=75, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6320593, Length=149, Percent_Identity=30.8724832214765, Blast_Score=75, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24582462, Length=492, Percent_Identity=28.4552845528455, Blast_Score=182, Evalue=6e-46,
Organism=Drosophila melanogaster, GI221458488, Length=566, Percent_Identity=24.3816254416961, Blast_Score=135, Evalue=5e-32,
Organism=Drosophila melanogaster, GI78706572, Length=159, Percent_Identity=32.7044025157233, Blast_Score=103, Evalue=3e-22,
Organism=Drosophila melanogaster, GI24585711, Length=154, Percent_Identity=30.5194805194805, Blast_Score=72, Evalue=7e-13,
Organism=Drosophila melanogaster, GI24585713, Length=154, Percent_Identity=30.5194805194805, Blast_Score=72, Evalue=7e-13,
Organism=Drosophila melanogaster, GI24585709, Length=154, Percent_Identity=30.5194805194805, Blast_Score=72, Evalue=8e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RF3_GEOMG (Q39Z86)

Other databases:

- EMBL:   CP000148
- RefSeq:   YP_383163.1
- ProteinModelPortal:   Q39Z86
- SMR:   Q39Z86
- STRING:   Q39Z86
- GeneID:   3739076
- GenomeReviews:   CP000148_GR
- KEGG:   gme:Gmet_0192
- NMPDR:   fig|269799.3.peg.223
- eggNOG:   COG4108
- HOGENOM:   HBG285678
- OMA:   HERTVEP
- PhylomeDB:   Q39Z86
- ProtClustDB:   PRK00741
- BioCyc:   GMET269799:GMET_0192-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00072
- InterPro:   IPR009022
- InterPro:   IPR004548
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR004161
- InterPro:   IPR009000
- PRINTS:   PR00315
- TIGRFAMs:   TIGR00503
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; SSF54980 EFG_III_V; SSF50447 Translat_factor

EC number: NA

Molecular weight: Translated: 59518; Mature: 59386

Theoretical pI: Translated: 6.28; Mature: 6.28

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKHNEQEIDRRRTFAIISHPDAGKTTITEKLLLFGGAIQQAGEVRARKAARHATSDWME
CCCCCHHHHHHHHEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MEKQRGISVTSSVMKFTYRNYEVNLLDTPGHNDFSEDTYRVLTAVDSALMVIDAVKGVES
HHHHCCCHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCH
QTIKLLDVCRLRHTPIMTFVNKLDREGRDPFELIDEIEKVLKIQCAPMTWPIGMGKRFRG
HHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHEEEECCCCCCCCCCCCCCC
TYHLYTKELIIFDAEAERGTGSIVSLSGLDDPRLDEILGSQAAELRGDIELLEGAAHPFE
EEEEEEEEEEEEECCCCCCCCCEEEECCCCCCHHHHHHCCHHHHHHCCHHHHHCCCCCCC
EEAYLAGLQTPVFFGSAINTFGVQQLLDTFVENAPAPLPREAVTRTVSPYEEPFAAFAFK
CHHHHHCCCCCHHCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCCCCEEEEEE
IQANMDPAHRDRIAFFRICSGKFTRGMKVRHVRLGREVAINNATIFMAQDRTHVDEAFPG
EECCCCHHHHHHEEEEEEECCCCCCCCEEEEEECCCEEEECCEEEEEECCCCCCCCCCCC
DIIGIHNHGTIKIGDTFTMGEELKFTGIPNFAPEHFRKVRLLDPLKSKALEKGLTQLAEE
CEEEEECCCEEEECCEEECCCCEEECCCCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHC
GTTQVFRPLMGADWIVGAVGILQFDVVMHRLEHEYNVKATYEPAAYATARWVTGEKKKLE
CHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHEEEECCCCHHHHH
EFQKKEVMSCYIDGEGNLAYLASSQWRLDNTMDNWKDLQFHATREHS
HHHHHCEEEEEECCCCCEEEEECCCEECCCCCCCCCCCEEEECCCCC
>Mature Secondary Structure 
TKHNEQEIDRRRTFAIISHPDAGKTTITEKLLLFGGAIQQAGEVRARKAARHATSDWME
CCCCHHHHHHHHEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MEKQRGISVTSSVMKFTYRNYEVNLLDTPGHNDFSEDTYRVLTAVDSALMVIDAVKGVES
HHHHCCCHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCH
QTIKLLDVCRLRHTPIMTFVNKLDREGRDPFELIDEIEKVLKIQCAPMTWPIGMGKRFRG
HHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHEEEECCCCCCCCCCCCCCC
TYHLYTKELIIFDAEAERGTGSIVSLSGLDDPRLDEILGSQAAELRGDIELLEGAAHPFE
EEEEEEEEEEEEECCCCCCCCCEEEECCCCCCHHHHHHCCHHHHHHCCHHHHHCCCCCCC
EEAYLAGLQTPVFFGSAINTFGVQQLLDTFVENAPAPLPREAVTRTVSPYEEPFAAFAFK
CHHHHHCCCCCHHCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCCCCEEEEEE
IQANMDPAHRDRIAFFRICSGKFTRGMKVRHVRLGREVAINNATIFMAQDRTHVDEAFPG
EECCCCHHHHHHEEEEEEECCCCCCCCEEEEEECCCEEEECCEEEEEECCCCCCCCCCCC
DIIGIHNHGTIKIGDTFTMGEELKFTGIPNFAPEHFRKVRLLDPLKSKALEKGLTQLAEE
CEEEEECCCEEEECCEEECCCCEEECCCCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHC
GTTQVFRPLMGADWIVGAVGILQFDVVMHRLEHEYNVKATYEPAAYATARWVTGEKKKLE
CHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHEEEECCCCHHHHH
EFQKKEVMSCYIDGEGNLAYLASSQWRLDNTMDNWKDLQFHATREHS
HHHHHCEEEEEECCCCCEEEEECCCEECCCCCCCCCCCEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA