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The map label for this gene is hemB [H]

Identifier: 78221413

GI number: 78221413

Start: 219554

End: 220531

Strand: Reverse

Name: hemB [H]

Synonym: Gmet_0189

Alternate gene names: 78221413

Gene position: 220531-219554 (Counterclockwise)

Preceding gene: 78221415

Following gene: 78221412

Centisome position: 5.52

GC content: 64.21

Gene sequence:

>978_bases
ATGTTTTTTCCCACGTTCCGCGCCCGCAGAATCAGGGGCAAAGAGGTCTTCCGCCGGATGGTCAGCGAGACCACCCTTTC
CGCCACCGACCTGATCTACCCGATGTTTTCCGCCTTCGGCACGGGGATCAGAAAAGAGATCTCCTCCATGCCGGGGATCT
ACCAGCAGTCCATCGAGCACATCGTCGCCGAGGCCCAGGAGGTGTACGAACTGGGGGTTCCCGCAGTGATCCTCTTCGGC
ATCCCCGAAACCAAGGACGCCGTGGGGAGCGACGCCTACGACGAGCACGGCATCATCCAGGAAACGATCCGGGCCATCAA
GAAGCAGGTGCCGGGGCTGGCGGTCATCACCGACGTCTGCATGTGCGAGTACACCGACCACGGTCACTGCGGCATCATTA
AAGATGGCGACGTGGACAACGACGAGACCCTGGAGCTCCTGGCCAAGGAAGCCCTCTCCCACGCAGAGGCAGGCGCCGAC
ATGGTGGCGCCGTCGGACATGATGGACGGCCGGGTCATGGCCATCCGCGAAATCCTCGACAACCACGGCTACAAGCACAT
CCCCATCATGAGCTACGCCGTGAAATACGCCTCGGGCTACTACGGCCCGTTCCGGGAGGCCGCCGAGTCGACGCCGCAAT
TCGGCGACCGCCGCTCCTACCAGATGGACCCGGGGAACCGCCGGGAGGCCGTCCGCGAGGCCCGCATGGACGTGGAGGAA
GGGGCCGACATCATCATGGTGAAGCCGGGGCTCCCCTACCTCGACATCGTCCGAGACCTGCGAGAAGAGTTCGACCTCCC
CGTGGCGGTCTACAACGTCTCCGGCGAGTACAGCATGATCAAGGCCGCCGGCCGGGCCGGCTGGATCGACGAGGAGCGGG
TCATGATGGAGACGCTGCTCTCCTTCAAGCGGGCCGGCGCCGACCTCATCCTCACCTACCACGCCAAGGAGGCGGCACGG
GTGCTGAAGAGAGGTTAG

Upstream 100 bases:

>100_bases
ACCGCCAAAAATGTTTGAGCCATGCCCCCCACTTGGAGTACTATCAATTAGTTGATGTTCCAGCAGATATCCCTATCAAA
CCACAAGGAGACATGACACC

Downstream 100 bases:

>100_bases
CCTGATTCCGTGATAAACTTGAAAGGCCTGCCGGTTGGCGGGCCTTTTTTCATGTCCATCTTTGAATAACACGGAGAGAC
GGAGCGCACAGAGAAAACCA

Product: delta-aminolevulinic acid dehydratase

Products: NA

Alternate protein names: ALAD; ALADH; Porphobilinogen synthase [H]

Number of amino acids: Translated: 325; Mature: 325

Protein sequence:

>325_residues
MFFPTFRARRIRGKEVFRRMVSETTLSATDLIYPMFSAFGTGIRKEISSMPGIYQQSIEHIVAEAQEVYELGVPAVILFG
IPETKDAVGSDAYDEHGIIQETIRAIKKQVPGLAVITDVCMCEYTDHGHCGIIKDGDVDNDETLELLAKEALSHAEAGAD
MVAPSDMMDGRVMAIREILDNHGYKHIPIMSYAVKYASGYYGPFREAAESTPQFGDRRSYQMDPGNRREAVREARMDVEE
GADIIMVKPGLPYLDIVRDLREEFDLPVAVYNVSGEYSMIKAAGRAGWIDEERVMMETLLSFKRAGADLILTYHAKEAAR
VLKRG

Sequences:

>Translated_325_residues
MFFPTFRARRIRGKEVFRRMVSETTLSATDLIYPMFSAFGTGIRKEISSMPGIYQQSIEHIVAEAQEVYELGVPAVILFG
IPETKDAVGSDAYDEHGIIQETIRAIKKQVPGLAVITDVCMCEYTDHGHCGIIKDGDVDNDETLELLAKEALSHAEAGAD
MVAPSDMMDGRVMAIREILDNHGYKHIPIMSYAVKYASGYYGPFREAAESTPQFGDRRSYQMDPGNRREAVREARMDVEE
GADIIMVKPGLPYLDIVRDLREEFDLPVAVYNVSGEYSMIKAAGRAGWIDEERVMMETLLSFKRAGADLILTYHAKEAAR
VLKRG
>Mature_325_residues
MFFPTFRARRIRGKEVFRRMVSETTLSATDLIYPMFSAFGTGIRKEISSMPGIYQQSIEHIVAEAQEVYELGVPAVILFG
IPETKDAVGSDAYDEHGIIQETIRAIKKQVPGLAVITDVCMCEYTDHGHCGIIKDGDVDNDETLELLAKEALSHAEAGAD
MVAPSDMMDGRVMAIREILDNHGYKHIPIMSYAVKYASGYYGPFREAAESTPQFGDRRSYQMDPGNRREAVREARMDVEE
GADIIMVKPGLPYLDIVRDLREEFDLPVAVYNVSGEYSMIKAAGRAGWIDEERVMMETLLSFKRAGADLILTYHAKEAAR
VLKRG

Specific function: Catalyzes an early step in the biosynthesis of tetrapyrroles. Binds two molecules of 5-aminolevulinate per subunit, each at a distinct site, and catalyzes their condensation to form porphobilinogen [H]

COG id: COG0113

COG function: function code H; Delta-aminolevulinic acid dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ALADH family [H]

Homologues:

Organism=Homo sapiens, GI189083849, Length=327, Percent_Identity=43.7308868501529, Blast_Score=274, Evalue=6e-74,
Organism=Escherichia coli, GI87081728, Length=313, Percent_Identity=50.7987220447284, Blast_Score=308, Evalue=2e-85,
Organism=Saccharomyces cerevisiae, GI6321398, Length=308, Percent_Identity=42.8571428571429, Blast_Score=237, Evalue=2e-63,
Organism=Drosophila melanogaster, GI21358291, Length=296, Percent_Identity=38.8513513513513, Blast_Score=239, Evalue=3e-63,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001731
- InterPro:   IPR013785 [H]

Pfam domain/function: PF00490 ALAD [H]

EC number: =4.2.1.24 [H]

Molecular weight: Translated: 36333; Mature: 36333

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: PS00169 D_ALA_DEHYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.9 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFFPTFRARRIRGKEVFRRMVSETTLSATDLIYPMFSAFGTGIRKEISSMPGIYQQSIEH
CCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCHHHHHHHH
IVAEAQEVYELGVPAVILFGIPETKDAVGSDAYDEHGIIQETIRAIKKQVPGLAVITDVC
HHHHHHHHHHCCCCEEEEECCCCCHHHCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHH
MCEYTDHGHCGIIKDGDVDNDETLELLAKEALSHAEAGADMVAPSDMMDGRVMAIREILD
HHCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCHHHHHHHHHH
NHGYKHIPIMSYAVKYASGYYGPFREAAESTPQFGDRRSYQMDPGNRREAVREARMDVEE
CCCCCCCHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHC
GADIIMVKPGLPYLDIVRDLREEFDLPVAVYNVSGEYSMIKAAGRAGWIDEERVMMETLL
CCCEEEECCCCCHHHHHHHHHHHCCCCEEEEECCCCHHHHHHCCCCCCCCHHHHHHHHHH
SFKRAGADLILTYHAKEAARVLKRG
HHHHCCCCEEEEECHHHHHHHHHCC
>Mature Secondary Structure
MFFPTFRARRIRGKEVFRRMVSETTLSATDLIYPMFSAFGTGIRKEISSMPGIYQQSIEH
CCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCHHHHHHHH
IVAEAQEVYELGVPAVILFGIPETKDAVGSDAYDEHGIIQETIRAIKKQVPGLAVITDVC
HHHHHHHHHHCCCCEEEEECCCCCHHHCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHH
MCEYTDHGHCGIIKDGDVDNDETLELLAKEALSHAEAGADMVAPSDMMDGRVMAIREILD
HHCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCHHHHHHHHHH
NHGYKHIPIMSYAVKYASGYYGPFREAAESTPQFGDRRSYQMDPGNRREAVREARMDVEE
CCCCCCCHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHC
GADIIMVKPGLPYLDIVRDLREEFDLPVAVYNVSGEYSMIKAAGRAGWIDEERVMMETLL
CCCEEEECCCCCHHHHHHHHHHHCCCCEEEEECCCCHHHHHHCCCCCCCCHHHHHHHHHH
SFKRAGADLILTYHAKEAARVLKRG
HHHHCCCCEEEEECHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8123787 [H]