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The map label for this gene is yeiE [H]

Identifier: 78221410

GI number: 78221410

Start: 216182

End: 217072

Strand: Reverse

Name: yeiE [H]

Synonym: Gmet_0186

Alternate gene names: 78221410

Gene position: 217072-216182 (Counterclockwise)

Preceding gene: 78221412

Following gene: 78221408

Centisome position: 5.43

GC content: 57.35

Gene sequence:

>891_bases
ATGGCTATTACCGTGAGGCAACTGGAAATATTTGAAAAGGTTGCCCGATGCGAGCATGTGACCCAGGCAAGCAGTCAGCT
CTTCATAACGCAGTCGGCGGTGAGCATGGCCATTGCCGAACTGGAGCGGCTGGCGGGGGCCCCGCTCTTCGAGCGCCACG
GCAAGCGCCTGCTCCTGAATGATCGGGGACGCAAGATCCTGCCGGAAGCCGTGGAGGTGATCAGAAGAATCCGGGCGATA
GAGCAGTTCCTCGAAGACTCCGCCGGCGAACCCCGGGGGGTCCTGACCATCGGGGCAAGCACCACCATCGGCAACTATAT
TCTGCCCGACATCGTTGCCGAATTTTCGAGGAACTACCCCCAGGCAAAGGTGTTGCTGACCATCGCGAATGCCCAGCAGA
TCGAGAATGCTCTGGAAAATGGGGAGCTCGACCTGGGACTGATCGAAGGGATTCCCCATTCCAGGGTTCTCGACACATCC
CCCTGGAAAAATGATGAATTGGTCGTCGTGGTTGGCAAAGGGCATCCGTGGGGGGGCAGAAAAAAGGCTTCTGTGGAGAT
GTTGCACGGCGCCCCCTGGATCATGCGTGAAAAGGGGTCCGGAACGAGGGAGGTTTTTGAGGCGGCAATGGCCCGAAAAG
GGATAGAGTTCTCAATCGCCCTGGAATTGGGGCATACCGAAGCCATCAAGAAGGCCACTGAAGCGGGCCTGGGGGTAGGA
TGCCTCTCGAAAATTGCCGTCGAAAGGGAGCTGGAACAGGGCTGGCTCGTGGAGGTTGCGTGCTCTCTTGACTTACGGAG
AACCCTTTCGGTCCAGACAAGGGAATGTCGATACCAGACGAGGTTATTGCAGGAATTTCTCTCCCTGTTGAACCAGTACC
GGGACACATGA

Upstream 100 bases:

>100_bases
TCCCCGATGCATGTTTTGATTGCATCATAGGCCATCTCGCATATAATAAAAAATGAGTAATATCTATCAATATGTTCAAT
ATTTTTTATAAGGGACTGCA

Downstream 100 bases:

>100_bases
CCAAACGACGCGCACGTGCGCATGACGGCTGGTCAGCCTGCTGCTGACGGGAACACCCCCTCACCGGGTCTTGCTCCCCC
ACAGCTTCCCCAGCCACGCA

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 296; Mature: 295

Protein sequence:

>296_residues
MAITVRQLEIFEKVARCEHVTQASSQLFITQSAVSMAIAELERLAGAPLFERHGKRLLLNDRGRKILPEAVEVIRRIRAI
EQFLEDSAGEPRGVLTIGASTTIGNYILPDIVAEFSRNYPQAKVLLTIANAQQIENALENGELDLGLIEGIPHSRVLDTS
PWKNDELVVVVGKGHPWGGRKKASVEMLHGAPWIMREKGSGTREVFEAAMARKGIEFSIALELGHTEAIKKATEAGLGVG
CLSKIAVERELEQGWLVEVACSLDLRRTLSVQTRECRYQTRLLQEFLSLLNQYRDT

Sequences:

>Translated_296_residues
MAITVRQLEIFEKVARCEHVTQASSQLFITQSAVSMAIAELERLAGAPLFERHGKRLLLNDRGRKILPEAVEVIRRIRAI
EQFLEDSAGEPRGVLTIGASTTIGNYILPDIVAEFSRNYPQAKVLLTIANAQQIENALENGELDLGLIEGIPHSRVLDTS
PWKNDELVVVVGKGHPWGGRKKASVEMLHGAPWIMREKGSGTREVFEAAMARKGIEFSIALELGHTEAIKKATEAGLGVG
CLSKIAVERELEQGWLVEVACSLDLRRTLSVQTRECRYQTRLLQEFLSLLNQYRDT
>Mature_295_residues
AITVRQLEIFEKVARCEHVTQASSQLFITQSAVSMAIAELERLAGAPLFERHGKRLLLNDRGRKILPEAVEVIRRIRAIE
QFLEDSAGEPRGVLTIGASTTIGNYILPDIVAEFSRNYPQAKVLLTIANAQQIENALENGELDLGLIEGIPHSRVLDTSP
WKNDELVVVVGKGHPWGGRKKASVEMLHGAPWIMREKGSGTREVFEAAMARKGIEFSIALELGHTEAIKKATEAGLGVGC
LSKIAVERELEQGWLVEVACSLDLRRTLSVQTRECRYQTRLLQEFLSLLNQYRDT

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788481, Length=293, Percent_Identity=41.6382252559727, Blast_Score=215, Evalue=3e-57,
Organism=Escherichia coli, GI1788296, Length=244, Percent_Identity=29.5081967213115, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI87082132, Length=239, Percent_Identity=28.8702928870293, Blast_Score=102, Evalue=2e-23,
Organism=Escherichia coli, GI1790399, Length=273, Percent_Identity=23.8095238095238, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI157672245, Length=224, Percent_Identity=27.6785714285714, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1788748, Length=250, Percent_Identity=25.2, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI145693105, Length=290, Percent_Identity=26.2068965517241, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1787530, Length=241, Percent_Identity=26.5560165975104, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1787601, Length=196, Percent_Identity=27.0408163265306, Blast_Score=78, Evalue=8e-16,
Organism=Escherichia coli, GI1787806, Length=173, Percent_Identity=24.8554913294798, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1786401, Length=300, Percent_Identity=22.6666666666667, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1787879, Length=280, Percent_Identity=25.7142857142857, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1788626, Length=238, Percent_Identity=28.5714285714286, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1788887, Length=213, Percent_Identity=26.7605633802817, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1789204, Length=289, Percent_Identity=22.4913494809689, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1790262, Length=258, Percent_Identity=24.031007751938, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI87081904, Length=145, Percent_Identity=30.3448275862069, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32906; Mature: 32775

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAITVRQLEIFEKVARCEHVTQASSQLFITQSAVSMAIAELERLAGAPLFERHGKRLLLN
CEEEHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHCCCHHHHCCCEEEEC
DRGRKILPEAVEVIRRIRAIEQFLEDSAGEPRGVLTIGASTTIGNYILPDIVAEFSRNYP
CCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHCCCHHHHHHHHHHHCCCC
QAKVLLTIANAQQIENALENGELDLGLIEGIPHSRVLDTSPWKNDELVVVVGKGHPWGGR
CEEEEEEECCHHHHHHHHHCCCCCEEHCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCC
KKASVEMLHGAPWIMREKGSGTREVFEAAMARKGIEFSIALELGHTEAIKKATEAGLGVG
CCCCEEHCCCCCEEEECCCCCHHHHHHHHHHHCCCEEEEEEEECCHHHHHHHHHCCCCHH
CLSKIAVERELEQGWLVEVACSLDLRRTLSVQTRECRYQTRLLQEFLSLLNQYRDT
HHHHHHHHHHHHCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AITVRQLEIFEKVARCEHVTQASSQLFITQSAVSMAIAELERLAGAPLFERHGKRLLLN
EEEHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHCCCHHHHCCCEEEEC
DRGRKILPEAVEVIRRIRAIEQFLEDSAGEPRGVLTIGASTTIGNYILPDIVAEFSRNYP
CCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHCCCHHHHHHHHHHHCCCC
QAKVLLTIANAQQIENALENGELDLGLIEGIPHSRVLDTSPWKNDELVVVVGKGHPWGGR
CEEEEEEECCHHHHHHHHHCCCCCEEHCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCC
KKASVEMLHGAPWIMREKGSGTREVFEAAMARKGIEFSIALELGHTEAIKKATEAGLGVG
CCCCEEHCCCCCEEEECCCCCHHHHHHHHHHHCCCEEEEEEEECCHHHHHHHHHCCCCHH
CLSKIAVERELEQGWLVEVACSLDLRRTLSVQTRECRYQTRLLQEFLSLLNQYRDT
HHHHHHHHHHHHCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]