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The map label for this gene is htrB [H]

Identifier: 78221318

GI number: 78221318

Start: 115137

End: 116111

Strand: Direct

Name: htrB [H]

Synonym: Gmet_0091

Alternate gene names: 78221318

Gene position: 115137-116111 (Clockwise)

Preceding gene: 78221317

Following gene: 78221321

Centisome position: 2.88

GC content: 66.77

Gene sequence:

>975_bases
ATGTCGATGGAGTCACCTGAACACGAGGCAGCACCCTCTCCCCCCCGCAACCGCACCAAGGAGACGATCCGCGCCATCAA
GTGGCGGCTGGGGCTAGTCCTGTTCGCCTTGGTCGCAATTCCGGTCCTCCTCCTGCCGCGCCGCCTGGCCGTGGGGCTCG
GCGCCCGCGGGGGCCGGCTCGTTTTCCTCCTCATGGGGAAGCTGCGGCGCAAGACCGTGGCACACATTGAGAGCGCCTAT
CCCTACCTCGGCACCCTGGAAGAGTGGGACCCCGCCGCCGGCACGCCGGAGGAGATCGCCCGGCGCACCTTCGCCAACAT
CGGCCGGACCGCTGTGGAGATCATTAAGCTCTATTTCGGCCAGGGAAAGGGACTTGTCGACCGGACGGAACTGCGGGGAT
TGGAGCACGTTCGGGCGGCAAAGGCGCGGGGGAAAGGGGTCATCTTCATCACCGGCCACTGCGACAACTGGGAGCTAGTG
GCCCACGCCTTCGGCACCAACGCCGAGGGGATGGCGGTGGTGGCGAGGCGGCAGAAGTTCGAGCCCCTGACACGGCTGCT
GGAACGGCTCCGCAACCGGCACGGCAACAGCATCATCTACTCCGAGGGTGCGGCCCGGCAGATCTTTTTCCGGCTCCGGA
AGAACGCGGCCATCGGCCTTCTCATGGATCAGTCAGTCCCTTTCCACGAGGGGGAGCTGGTGGAGTTCCTCGGAAGAAAG
GCCTGGACCACCACCATGCCGGCCCTCATGGCGGCCAGGACCGGCGCCGCCCTAGTCCCCGGTTTCGGCCACCGGGAGGG
GGACCGCCACGTGGTGGAGTTCTTCCCTGAAATAGCCCCCGATCCTTCCGGCGATCCCATCGCCACCACCCGCCTTCTCA
ACCGGGCCATCGAGCAGTACATCGCCCGCCACCCCGACCAGTGGCTCTGGGTCTACAACCGCTGGAAGCGGGTGACCCGG
GAGCAGCAAGGCTAG

Upstream 100 bases:

>100_bases
GCGCAAGGCGGCCCTCCTGGCCCTGGAGCGGGGCGGCGGGTTGCCGCCGGGTTGATGCCAGGCGGTTCCCCTCCCGCTAA
AACTGTGCTAGGGTGAGTCC

Downstream 100 bases:

>100_bases
GCCGGCCGTCTCCCGGCGGCGTCCTTCAGCTTCTCCATATCGAAAATCACCACCCGGCTCCCTTCCACCTCGATGAGCCC
CTCCTCCTTCAGCTTCCGGA

Product: lipid A biosynthesis acyltransferase

Products: NA

Alternate protein names: Heat shock protein B [H]

Number of amino acids: Translated: 324; Mature: 323

Protein sequence:

>324_residues
MSMESPEHEAAPSPPRNRTKETIRAIKWRLGLVLFALVAIPVLLLPRRLAVGLGARGGRLVFLLMGKLRRKTVAHIESAY
PYLGTLEEWDPAAGTPEEIARRTFANIGRTAVEIIKLYFGQGKGLVDRTELRGLEHVRAAKARGKGVIFITGHCDNWELV
AHAFGTNAEGMAVVARRQKFEPLTRLLERLRNRHGNSIIYSEGAARQIFFRLRKNAAIGLLMDQSVPFHEGELVEFLGRK
AWTTTMPALMAARTGAALVPGFGHREGDRHVVEFFPEIAPDPSGDPIATTRLLNRAIEQYIARHPDQWLWVYNRWKRVTR
EQQG

Sequences:

>Translated_324_residues
MSMESPEHEAAPSPPRNRTKETIRAIKWRLGLVLFALVAIPVLLLPRRLAVGLGARGGRLVFLLMGKLRRKTVAHIESAY
PYLGTLEEWDPAAGTPEEIARRTFANIGRTAVEIIKLYFGQGKGLVDRTELRGLEHVRAAKARGKGVIFITGHCDNWELV
AHAFGTNAEGMAVVARRQKFEPLTRLLERLRNRHGNSIIYSEGAARQIFFRLRKNAAIGLLMDQSVPFHEGELVEFLGRK
AWTTTMPALMAARTGAALVPGFGHREGDRHVVEFFPEIAPDPSGDPIATTRLLNRAIEQYIARHPDQWLWVYNRWKRVTR
EQQG
>Mature_323_residues
SMESPEHEAAPSPPRNRTKETIRAIKWRLGLVLFALVAIPVLLLPRRLAVGLGARGGRLVFLLMGKLRRKTVAHIESAYP
YLGTLEEWDPAAGTPEEIARRTFANIGRTAVEIIKLYFGQGKGLVDRTELRGLEHVRAAKARGKGVIFITGHCDNWELVA
HAFGTNAEGMAVVARRQKFEPLTRLLERLRNRHGNSIIYSEGAARQIFFRLRKNAAIGLLMDQSVPFHEGELVEFLGRKA
WTTTMPALMAARTGAALVPGFGHREGDRHVVEFFPEIAPDPSGDPIATTRLLNRAIEQYIARHPDQWLWVYNRWKRVTRE
QQG

Specific function: Acylates the intermediate (KDO)2-lipid IVA to form (KDO)2-(lauroyl)-lipid IVA. Has 10 fold selectivity for lauroyl- ACP over myristoyl-ACP [H]

COG id: COG1560

COG function: function code M; Lauroyl/myristoyl acyltransferase

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the htrB/msbB family [H]

Homologues:

Organism=Escherichia coli, GI1787292, Length=290, Percent_Identity=24.8275862068966, Blast_Score=80, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004960
- InterPro:   IPR011920 [H]

Pfam domain/function: PF03279 Lip_A_acyltrans [H]

EC number: 2.3.1.-

Molecular weight: Translated: 36461; Mature: 36330

Theoretical pI: Translated: 10.93; Mature: 10.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSMESPEHEAAPSPPRNRTKETIRAIKWRLGLVLFALVAIPVLLLPRRLAVGLGARGGRL
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
VFLLMGKLRRKTVAHIESAYPYLGTLEEWDPAAGTPEEIARRTFANIGRTAVEIIKLYFG
HHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHC
QGKGLVDRTELRGLEHVRAAKARGKGVIFITGHCDNWELVAHAFGTNAEGMAVVARRQKF
CCCCCCCHHHHHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHCCCCCCHHHHHHHHHH
EPLTRLLERLRNRHGNSIIYSEGAARQIFFRLRKNAAIGLLMDQSVPFHEGELVEFLGRK
HHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHHHCCH
AWTTTMPALMAARTGAALVPGFGHREGDRHVVEFFPEIAPDPSGDPIATTRLLNRAIEQY
HHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
IARHPDQWLWVYNRWKRVTREQQG
HHHCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SMESPEHEAAPSPPRNRTKETIRAIKWRLGLVLFALVAIPVLLLPRRLAVGLGARGGRL
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
VFLLMGKLRRKTVAHIESAYPYLGTLEEWDPAAGTPEEIARRTFANIGRTAVEIIKLYFG
HHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHC
QGKGLVDRTELRGLEHVRAAKARGKGVIFITGHCDNWELVAHAFGTNAEGMAVVARRQKF
CCCCCCCHHHHHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHCCCCCCHHHHHHHHHH
EPLTRLLERLRNRHGNSIIYSEGAARQIFFRLRKNAAIGLLMDQSVPFHEGELVEFLGRK
HHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHHHCCH
AWTTTMPALMAARTGAALVPGFGHREGDRHVVEFFPEIAPDPSGDPIATTRLLNRAIEQY
HHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
IARHPDQWLWVYNRWKRVTREQQG
HHHCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Acyltransferases [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]