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The map label for this gene is cysS

Identifier: 78221284

GI number: 78221284

Start: 70681

End: 72138

Strand: Reverse

Name: cysS

Synonym: Gmet_0057

Alternate gene names: 78221284

Gene position: 72138-70681 (Counterclockwise)

Preceding gene: 78221285

Following gene: 78221281

Centisome position: 1.8

GC content: 60.84

Gene sequence:

>1458_bases
ATGTCTTTGCGCGTCTACAATACGCTTTCCGGAACCAAGGAAGAATTCACCCCCCTCGTGCCGGGAAGGGTCTCCATGTA
TGTCTGCGGCGTCACGGTCTACGACCACTGCCACATCGGCCACGCCCGGGCCAACGTGGTTTTCGACATGATCTACCGCT
ACCTGCGCCACGCGGGATATGACGTTACCTACGTCCGCAACTACACCGACGTGGACGACAAGATCATCAACCGGGCCAAC
CAGGAAGGTGTTCCCTACAACGCCATCTCCGAGCGCTTCATCGCCGAGTTCGACCGGGATATGGCGGCCCTGGGGCTCGA
TCTCCCCACCCACCAGCCTAAGGCTACGGAACACATCGCCGAGATGATCGATGTCATCAGCCGCCTCATCGAGAAAGGCT
TTGCCTACGCAGCGGACGGCGACGTCTACTTCTGTGTGGAAAAGTTTGATCCCTACCTGAAGCTCTCCAAGCGCAACCTG
GACGAGATGCAGGCCGGAGCCCGGATCGAGGTGGGGGAGAAGAAGCGCCACCCCATGGATTTCGCCCTCTGGAAGGGATC
CAAACCGGGTGAGCCATTCTGGGAGTCCCCCTGGGGCCAGGGGCGGCCGGGATGGCACATCGAGTGCTCGGCCATGAGCA
TGAAGTACCTGGGGGAGACCTTCGACATCCATGGCGGCGGCAAAGACCTGGTCTTTCCCCACCACGAGAACGAGATCGCC
CAGTCCGAGGCGGCCAACGGCAAACCCTTTGCCCACTACTGGATCCACAACGGCTTCGTGAACATCAACTCCGAGAAGAT
GAGCAAGTCCCTAGGGAACTTCTTCACCATCAAGGAGGTGCTGGAGAAGTACGATGCCGAGGTGCTCCGCTTCTTCCTCC
TCTCGGCCCACTACCGCTCCCCCATCGACTTCTCCGACCAGAACCTGCGGGAGGCCGAGGCGGGGCTGGAGCGGATCTAC
GGGACCCTTGCGGGAATCGATGAGAATCTGACAAAAGGAGCGGAGGGTACGGCAGAAGAGACTGACAGGGAGCTGGCCGA
AAAGGCGGTCACCTTTCCCGAGCGTTTCCGCGAAGCCATGGATGATGACTTCAACACGGCTCAGGCCCTAGGCTTCGTTT
TCGATCTGGTGAGGTCGGCAAACCGGGTGCTGGGGGAAGGAAAGGGAGGAGGGAGCAATCGCGCTCTCCTGGCAGAGGCA
CGGGATCGCATCCGGAAGGTGGGGGCGGTGCTCGGCATCTTCACCTCCGAGCCCCGTGCGTTCGCGGAGCGGCTCAAAAA
CCGCAAGGCTGCGGAGCTTCCCATTACCGCCGAGGAGATTGAGCGGCTAATCGCGGAACGGATTGCCGCCCGCGCAAACC
GGGACTTCAAACGCGCCGACGAAATCCGTGACAGTCTCGCCGCAAAAGGGATCATGCTGCTCGACTCCCCCCAAGGGACG
ACCTGGAAGGTGAAATGA

Upstream 100 bases:

>100_bases
GTTGACCTGAAGGATTCACGACCAGGGGCGCCGGTCTTCAACCGGACGGTGACCCTGCGGGATTCCTGGACCAAGAAATA
AACTCAAAGAGGTTAGTTGC

Downstream 100 bases:

>100_bases
CATATGGTTGCCACAGGATTCCCATCCCCTGTGACACCTTTGGCACAATCAGCTTAAGAAAAAGGGCCGCAAGTAGCGGC
CCTTTTTTCTTAAGCGGCCT

Product: cysteinyl-tRNA synthetase

Products: NA

Alternate protein names: Cysteine--tRNA ligase; CysRS

Number of amino acids: Translated: 485; Mature: 484

Protein sequence:

>485_residues
MSLRVYNTLSGTKEEFTPLVPGRVSMYVCGVTVYDHCHIGHARANVVFDMIYRYLRHAGYDVTYVRNYTDVDDKIINRAN
QEGVPYNAISERFIAEFDRDMAALGLDLPTHQPKATEHIAEMIDVISRLIEKGFAYAADGDVYFCVEKFDPYLKLSKRNL
DEMQAGARIEVGEKKRHPMDFALWKGSKPGEPFWESPWGQGRPGWHIECSAMSMKYLGETFDIHGGGKDLVFPHHENEIA
QSEAANGKPFAHYWIHNGFVNINSEKMSKSLGNFFTIKEVLEKYDAEVLRFFLLSAHYRSPIDFSDQNLREAEAGLERIY
GTLAGIDENLTKGAEGTAEETDRELAEKAVTFPERFREAMDDDFNTAQALGFVFDLVRSANRVLGEGKGGGSNRALLAEA
RDRIRKVGAVLGIFTSEPRAFAERLKNRKAAELPITAEEIERLIAERIAARANRDFKRADEIRDSLAAKGIMLLDSPQGT
TWKVK

Sequences:

>Translated_485_residues
MSLRVYNTLSGTKEEFTPLVPGRVSMYVCGVTVYDHCHIGHARANVVFDMIYRYLRHAGYDVTYVRNYTDVDDKIINRAN
QEGVPYNAISERFIAEFDRDMAALGLDLPTHQPKATEHIAEMIDVISRLIEKGFAYAADGDVYFCVEKFDPYLKLSKRNL
DEMQAGARIEVGEKKRHPMDFALWKGSKPGEPFWESPWGQGRPGWHIECSAMSMKYLGETFDIHGGGKDLVFPHHENEIA
QSEAANGKPFAHYWIHNGFVNINSEKMSKSLGNFFTIKEVLEKYDAEVLRFFLLSAHYRSPIDFSDQNLREAEAGLERIY
GTLAGIDENLTKGAEGTAEETDRELAEKAVTFPERFREAMDDDFNTAQALGFVFDLVRSANRVLGEGKGGGSNRALLAEA
RDRIRKVGAVLGIFTSEPRAFAERLKNRKAAELPITAEEIERLIAERIAARANRDFKRADEIRDSLAAKGIMLLDSPQGT
TWKVK
>Mature_484_residues
SLRVYNTLSGTKEEFTPLVPGRVSMYVCGVTVYDHCHIGHARANVVFDMIYRYLRHAGYDVTYVRNYTDVDDKIINRANQ
EGVPYNAISERFIAEFDRDMAALGLDLPTHQPKATEHIAEMIDVISRLIEKGFAYAADGDVYFCVEKFDPYLKLSKRNLD
EMQAGARIEVGEKKRHPMDFALWKGSKPGEPFWESPWGQGRPGWHIECSAMSMKYLGETFDIHGGGKDLVFPHHENEIAQ
SEAANGKPFAHYWIHNGFVNINSEKMSKSLGNFFTIKEVLEKYDAEVLRFFLLSAHYRSPIDFSDQNLREAEAGLERIYG
TLAGIDENLTKGAEGTAEETDRELAEKAVTFPERFREAMDDDFNTAQALGFVFDLVRSANRVLGEGKGGGSNRALLAEAR
DRIRKVGAVLGIFTSEPRAFAERLKNRKAAELPITAEEIERLIAERIAARANRDFKRADEIRDSLAAKGIMLLDSPQGTT
WKVK

Specific function: Unknown

COG id: COG0215

COG function: function code J; Cysteinyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI13375695, Length=388, Percent_Identity=39.9484536082474, Blast_Score=262, Evalue=6e-70,
Organism=Homo sapiens, GI303304987, Length=231, Percent_Identity=42.8571428571429, Blast_Score=194, Evalue=1e-49,
Organism=Homo sapiens, GI10835051, Length=241, Percent_Identity=41.0788381742739, Blast_Score=194, Evalue=1e-49,
Organism=Homo sapiens, GI21269877, Length=241, Percent_Identity=41.0788381742739, Blast_Score=194, Evalue=1e-49,
Organism=Homo sapiens, GI62240992, Length=231, Percent_Identity=42.8571428571429, Blast_Score=194, Evalue=1e-49,
Organism=Escherichia coli, GI1786737, Length=484, Percent_Identity=51.2396694214876, Blast_Score=481, Evalue=1e-137,
Organism=Caenorhabditis elegans, GI17509795, Length=369, Percent_Identity=34.4173441734417, Blast_Score=181, Evalue=9e-46,
Organism=Caenorhabditis elegans, GI17509797, Length=369, Percent_Identity=34.4173441734417, Blast_Score=181, Evalue=9e-46,
Organism=Saccharomyces cerevisiae, GI6324082, Length=341, Percent_Identity=35.1906158357771, Blast_Score=203, Evalue=4e-53,
Organism=Drosophila melanogaster, GI19922218, Length=505, Percent_Identity=33.4653465346535, Blast_Score=242, Evalue=4e-64,
Organism=Drosophila melanogaster, GI19922364, Length=358, Percent_Identity=36.3128491620112, Blast_Score=199, Evalue=3e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYC_GEOMG (Q39ZL8)

Other databases:

- EMBL:   CP000148
- RefSeq:   YP_383031.1
- ProteinModelPortal:   Q39ZL8
- SMR:   Q39ZL8
- STRING:   Q39ZL8
- GeneID:   3738545
- GenomeReviews:   CP000148_GR
- KEGG:   gme:Gmet_0057
- NMPDR:   fig|269799.3.peg.120
- eggNOG:   COG0215
- HOGENOM:   HBG327651
- OMA:   DFDALNM
- PhylomeDB:   Q39ZL8
- ProtClustDB:   PRK00260
- BioCyc:   GMET269799:GMET_0057-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00041_B
- InterPro:   IPR015803
- InterPro:   IPR015273
- InterPro:   IPR014729
- InterPro:   IPR009080
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR10890
- PRINTS:   PR00983
- SMART:   SM00840
- TIGRFAMs:   TIGR00435

Pfam domain/function: PF09190 DALR_2; PF01406 tRNA-synt_1e; SSF47323 tRNAsyn_1a_bind

EC number: =6.1.1.16

Molecular weight: Translated: 54737; Mature: 54606

Theoretical pI: Translated: 6.05; Mature: 6.05

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 269-269

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLRVYNTLSGTKEEFTPLVPGRVSMYVCGVTVYDHCHIGHARANVVFDMIYRYLRHAGY
CCEEEEECCCCCHHHCCCCCCCCEEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCC
DVTYVRNYTDVDDKIINRANQEGVPYNAISERFIAEFDRDMAALGLDLPTHQPKATEHIA
CEEEECCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHH
EMIDVISRLIEKGFAYAADGDVYFCVEKFDPYLKLSKRNLDEMQAGARIEVGEKKRHPMD
HHHHHHHHHHHCCCCEEECCCEEEEEECCCCHHHHHHCCHHHHHCCCEEEECCCCCCCCC
FALWKGSKPGEPFWESPWGQGRPGWHIECSAMSMKYLGETFDIHGGGKDLVFPHHENEIA
EEEECCCCCCCCCCCCCCCCCCCCCEEEEHHHHHHHCCCEEEECCCCCCCCCCCCCCHHH
QSEAANGKPFAHYWIHNGFVNINSEKMSKSLGNFFTIKEVLEKYDAEVLRFFLLSAHYRS
HHHCCCCCCEEEEEEECCEEEECHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
PIDFSDQNLREAEAGLERIYGTLAGIDENLTKGAEGTAEETDRELAEKAVTFPERFREAM
CCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCHHHHHHHHHHHHCCCHHHHHHHH
DDDFNTAQALGFVFDLVRSANRVLGEGKGGGSNRALLAEARDRIRKVGAVLGIFTSEPRA
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHH
FAERLKNRKAAELPITAEEIERLIAERIAARANRDFKRADEIRDSLAAKGIMLLDSPQGT
HHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCEEEEECCCCC
TWKVK
EEEEC
>Mature Secondary Structure 
SLRVYNTLSGTKEEFTPLVPGRVSMYVCGVTVYDHCHIGHARANVVFDMIYRYLRHAGY
CEEEEECCCCCHHHCCCCCCCCEEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCC
DVTYVRNYTDVDDKIINRANQEGVPYNAISERFIAEFDRDMAALGLDLPTHQPKATEHIA
CEEEECCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHH
EMIDVISRLIEKGFAYAADGDVYFCVEKFDPYLKLSKRNLDEMQAGARIEVGEKKRHPMD
HHHHHHHHHHHCCCCEEECCCEEEEEECCCCHHHHHHCCHHHHHCCCEEEECCCCCCCCC
FALWKGSKPGEPFWESPWGQGRPGWHIECSAMSMKYLGETFDIHGGGKDLVFPHHENEIA
EEEECCCCCCCCCCCCCCCCCCCCCEEEEHHHHHHHCCCEEEECCCCCCCCCCCCCCHHH
QSEAANGKPFAHYWIHNGFVNINSEKMSKSLGNFFTIKEVLEKYDAEVLRFFLLSAHYRS
HHHCCCCCCEEEEEEECCEEEECHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
PIDFSDQNLREAEAGLERIYGTLAGIDENLTKGAEGTAEETDRELAEKAVTFPERFREAM
CCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCHHHHHHHHHHHHCCCHHHHHHHH
DDDFNTAQALGFVFDLVRSANRVLGEGKGGGSNRALLAEARDRIRKVGAVLGIFTSEPRA
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHH
FAERLKNRKAAELPITAEEIERLIAERIAARANRDFKRADEIRDSLAAKGIMLLDSPQGT
HHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCEEEEECCCCC
TWKVK
EEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA