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The map label for this gene is anfA [H]

Identifier: 78221282

GI number: 78221282

Start: 67895

End: 70165

Strand: Direct

Name: anfA [H]

Synonym: Gmet_0055

Alternate gene names: 78221282

Gene position: 67895-70165 (Clockwise)

Preceding gene: 78221279

Following gene: 78221283

Centisome position: 1.7

GC content: 61.65

Gene sequence:

>2271_bases
ATGGAACCGATCATTACCGTAAAAGAGAAGTGCCGTAAATGCTACTGCTGTGTCCGGAGTTGCCCGGTCAAGGCGATCAA
GGTGGCCAAGAGCTACACCGAGATCATCGTCGACCGGTGCATCGGCTGCGGCAACTGTCTCAGCAACTGCCCCCAGCAGG
CAAAGATGGTGGCCGACAAGGTGGGGGTGACCGAAAAGCTCCTCTCCTCGGGGGAGGAAGTTATCGCCGTGCTCGGATCC
TCTTTTCCCGCCTTTTTCCACAATGTTACCCCCGGTCAGTTGGTGGCGGGCCTCAGGAAGATCGGCTTTGCCGAGGTCCA
CGAAGGATCCTATGGGGCCGAACTGATTGCCGACGATTATGCCCGAATCACGTCGGAAAAGGGACACCCCCGTATCTCGT
CCCACTGCCCTGCCATCGTCGATCTCATCGAGCGCCACTACCCGAAGCTCGTGGGGAACCTCGTGCCGGTGGTGTCCCCC
ATGGTGGCCATGGGGCGGTATCTCAAGGGGACCCTGGGGCAGCACGTGAGGGTTGTCTACATAAGCTCCTGCGTTGCCAA
CAAGCTGGAAACGCAGACCCAGGAGACCCGCGGCGCCGTTGACATCGTTCTGACCTACCGGGAACTGGAGGGTATCTTCC
GGAGCCGCCAGATAGCCCTGCCGGCCCTTGCCGACGAGCCCCTCGACGGAATCAGGCCGGGGGCCGGCCGGCTTTTTCCC
ATTGCCGACGGGACGTTCCGGGCCTTCGGCATCCCTTTTGATCCCCTGGACACCGAGATCGTCGCCGCCTGTGGCGAGGT
GAACGTCATGGGTATCATCAATGACCTGGCAGCTGGCCGCATCTCCCCCCGGATCGCCGACCTCCGTTTCTGCTACGACG
GCTGCATCGGCGGCCCCGGCAGAAACCGGGCGCTTACGGAGTTTTACCGCCGCAACCGGGTGATTGCCCACTTCAAGCAG
GAGGTCCCCTGCCGGACGGTCCCCAATTCCCTCCTTGAGGCAGGGCGGGTCTCCTTTGGCCGCAGCTTCGCCAGCAAGTA
CGCGAAACTGGAGGCCCCCAAGGCGAACGACGTCCGCAAGATCCTCAACGCCACCAACAAGTTCACGGTGAAGGACGAGC
TCAACTGCCGGGCCTGCGGTTACCGCACCTGCCGCGAGTACGCTGTGGCAGTCTTTCAGGGGCTCGCGGAGATTGAGATG
TGCCTCCCTTACAACCTCCAGCAACTGGAGGAGGACCGGGGGCGCCTCATTCAGAAGTACGAGTTGGCGCGGCGGGAACT
GGAACGGGAGTACGGGGACGAGTTCATCGTGGGCAACGACCGCAAGACCCTGGATGTGCTCGGCCTCATCAAACAGGTGG
GACCCACCCCCACAACGGTTCTCATTCGGGGGGAGTCAGGTACCGGCAAGGAGCTCACCGCCCGGGCGATCCACCGCTAC
AGCAAGCGCAACGACAAACCGCTGGTTACCGTCAACTGCACCACTATCACCGACTCTCTCCTGGAGAGCGAACTCTTTGG
CCACAAGCGGGGAGCCTTCACCGGTGCCGTTGCCGACAAGAAAGGGCTCTTTGAGGCCGCCGACGGGGGGACGATCTTCC
TCGATGAGATCGGCGACATAACCCCCAAGCTCCAGGCGGAGCTCTTGCGGGTTCTCGATATGGGGGAGGTGCGCCCCGTG
GGGGGGACTGCAGCAAAAAAGGTGGATGTGCGCCTCATTGCCGCCACCAACAAGAACCTGGAACAGGGGGTCCGCGAGGG
GTGGTTCCGGGAGGATCTCTATTACCGGCTCAACGTCTTCACCATTACCATGCCCCCCCTGCGAAGCCGCGTCGAGTCGA
TTCCCATTCTGGTCCACCACTTTATGGACAAGGCAAGCACCAAGCTCAACAAGCGGATGGTCGGCATCGAGGACCGTGCC
GTCAAGGCGCTCACAAAGTACCCGTGGCCCGGCAATATCCGTGAGATGCAGAACGTCATCGAGCGGGCTGCCGTTCTCAC
CCATGACGGCGTGATCCGGGTCGAGAACTTTCCCCTTGCCCTCTCTGAGGGGCTTGAGGAGGGGTTCGCCACGGGGCTCG
ACATCCACGCAGCATCGTTTCGGTCCGAGCGGGAGCAACATATGGGGAAGCTGGAGAAAAAGCTTATACAGCGCTACCTG
ACGGAGGCCAACGGCAATATTTCGCGGGCGGCGAAGCTGGCAAACATCCCCCGGCGGACCTTTTATCGGCTTCTCGATAA
GTACCGCCTCAGGGAACGGGATGTTCGCTAA

Upstream 100 bases:

>100_bases
GTGCCATTGGTTGCACAGGGAGGCGGGCCTGGTATATGATCCAGCGGTGGATCAGGCAATCAACAGAGTGTCAGGCCTAA
CATTATCAGCTGGGAGAACC

Downstream 100 bases:

>100_bases
CTCCTTGATGGCGCAGCGTAAACAAAGTGTGCACTTTTGGCCAACTCTGTGCCATCGGTGACACAATTGTGTATACAGAA
TATTTTATTAATTCAAACCA

Product: sigma-54 dependent trancsriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 756; Mature: 756

Protein sequence:

>756_residues
MEPIITVKEKCRKCYCCVRSCPVKAIKVAKSYTEIIVDRCIGCGNCLSNCPQQAKMVADKVGVTEKLLSSGEEVIAVLGS
SFPAFFHNVTPGQLVAGLRKIGFAEVHEGSYGAELIADDYARITSEKGHPRISSHCPAIVDLIERHYPKLVGNLVPVVSP
MVAMGRYLKGTLGQHVRVVYISSCVANKLETQTQETRGAVDIVLTYRELEGIFRSRQIALPALADEPLDGIRPGAGRLFP
IADGTFRAFGIPFDPLDTEIVAACGEVNVMGIINDLAAGRISPRIADLRFCYDGCIGGPGRNRALTEFYRRNRVIAHFKQ
EVPCRTVPNSLLEAGRVSFGRSFASKYAKLEAPKANDVRKILNATNKFTVKDELNCRACGYRTCREYAVAVFQGLAEIEM
CLPYNLQQLEEDRGRLIQKYELARRELEREYGDEFIVGNDRKTLDVLGLIKQVGPTPTTVLIRGESGTGKELTARAIHRY
SKRNDKPLVTVNCTTITDSLLESELFGHKRGAFTGAVADKKGLFEAADGGTIFLDEIGDITPKLQAELLRVLDMGEVRPV
GGTAAKKVDVRLIAATNKNLEQGVREGWFREDLYYRLNVFTITMPPLRSRVESIPILVHHFMDKASTKLNKRMVGIEDRA
VKALTKYPWPGNIREMQNVIERAAVLTHDGVIRVENFPLALSEGLEEGFATGLDIHAASFRSEREQHMGKLEKKLIQRYL
TEANGNISRAAKLANIPRRTFYRLLDKYRLRERDVR

Sequences:

>Translated_756_residues
MEPIITVKEKCRKCYCCVRSCPVKAIKVAKSYTEIIVDRCIGCGNCLSNCPQQAKMVADKVGVTEKLLSSGEEVIAVLGS
SFPAFFHNVTPGQLVAGLRKIGFAEVHEGSYGAELIADDYARITSEKGHPRISSHCPAIVDLIERHYPKLVGNLVPVVSP
MVAMGRYLKGTLGQHVRVVYISSCVANKLETQTQETRGAVDIVLTYRELEGIFRSRQIALPALADEPLDGIRPGAGRLFP
IADGTFRAFGIPFDPLDTEIVAACGEVNVMGIINDLAAGRISPRIADLRFCYDGCIGGPGRNRALTEFYRRNRVIAHFKQ
EVPCRTVPNSLLEAGRVSFGRSFASKYAKLEAPKANDVRKILNATNKFTVKDELNCRACGYRTCREYAVAVFQGLAEIEM
CLPYNLQQLEEDRGRLIQKYELARRELEREYGDEFIVGNDRKTLDVLGLIKQVGPTPTTVLIRGESGTGKELTARAIHRY
SKRNDKPLVTVNCTTITDSLLESELFGHKRGAFTGAVADKKGLFEAADGGTIFLDEIGDITPKLQAELLRVLDMGEVRPV
GGTAAKKVDVRLIAATNKNLEQGVREGWFREDLYYRLNVFTITMPPLRSRVESIPILVHHFMDKASTKLNKRMVGIEDRA
VKALTKYPWPGNIREMQNVIERAAVLTHDGVIRVENFPLALSEGLEEGFATGLDIHAASFRSEREQHMGKLEKKLIQRYL
TEANGNISRAAKLANIPRRTFYRLLDKYRLRERDVR
>Mature_756_residues
MEPIITVKEKCRKCYCCVRSCPVKAIKVAKSYTEIIVDRCIGCGNCLSNCPQQAKMVADKVGVTEKLLSSGEEVIAVLGS
SFPAFFHNVTPGQLVAGLRKIGFAEVHEGSYGAELIADDYARITSEKGHPRISSHCPAIVDLIERHYPKLVGNLVPVVSP
MVAMGRYLKGTLGQHVRVVYISSCVANKLETQTQETRGAVDIVLTYRELEGIFRSRQIALPALADEPLDGIRPGAGRLFP
IADGTFRAFGIPFDPLDTEIVAACGEVNVMGIINDLAAGRISPRIADLRFCYDGCIGGPGRNRALTEFYRRNRVIAHFKQ
EVPCRTVPNSLLEAGRVSFGRSFASKYAKLEAPKANDVRKILNATNKFTVKDELNCRACGYRTCREYAVAVFQGLAEIEM
CLPYNLQQLEEDRGRLIQKYELARRELEREYGDEFIVGNDRKTLDVLGLIKQVGPTPTTVLIRGESGTGKELTARAIHRY
SKRNDKPLVTVNCTTITDSLLESELFGHKRGAFTGAVADKKGLFEAADGGTIFLDEIGDITPKLQAELLRVLDMGEVRPV
GGTAAKKVDVRLIAATNKNLEQGVREGWFREDLYYRLNVFTITMPPLRSRVESIPILVHHFMDKASTKLNKRMVGIEDRA
VKALTKYPWPGNIREMQNVIERAAVLTHDGVIRVENFPLALSEGLEEGFATGLDIHAASFRSEREQHMGKLEKKLIQRYL
TEANGNISRAAKLANIPRRTFYRLLDKYRLRERDVR

Specific function: AnfA is essential for nitrogen fixation under Mo- and V- deficient conditions. It is required for the regulation of nitrogenase 3 transcription. Interacts with sigma-54 [H]

COG id: COG3604

COG function: function code KT; Transcriptional regulator containing GAF, AAA-type ATPase, and DNA binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=360, Percent_Identity=40, Blast_Score=259, Evalue=4e-70,
Organism=Escherichia coli, GI1790437, Length=312, Percent_Identity=45.5128205128205, Blast_Score=257, Evalue=2e-69,
Organism=Escherichia coli, GI87082117, Length=308, Percent_Identity=45.7792207792208, Blast_Score=251, Evalue=1e-67,
Organism=Escherichia coli, GI1788905, Length=323, Percent_Identity=41.4860681114551, Blast_Score=249, Evalue=4e-67,
Organism=Escherichia coli, GI1789233, Length=321, Percent_Identity=42.6791277258567, Blast_Score=247, Evalue=2e-66,
Organism=Escherichia coli, GI1789087, Length=302, Percent_Identity=43.3774834437086, Blast_Score=236, Evalue=4e-63,
Organism=Escherichia coli, GI1790299, Length=324, Percent_Identity=41.6666666666667, Blast_Score=232, Evalue=5e-62,
Organism=Escherichia coli, GI87082152, Length=218, Percent_Identity=46.7889908256881, Blast_Score=205, Evalue=9e-54,
Organism=Escherichia coli, GI87081872, Length=335, Percent_Identity=38.2089552238806, Blast_Score=201, Evalue=2e-52,
Organism=Escherichia coli, GI1786524, Length=300, Percent_Identity=42.6666666666667, Blast_Score=194, Evalue=1e-50,
Organism=Escherichia coli, GI1787583, Length=228, Percent_Identity=42.5438596491228, Blast_Score=177, Evalue=2e-45,
Organism=Escherichia coli, GI87081858, Length=310, Percent_Identity=31.2903225806452, Blast_Score=142, Evalue=7e-35,
Organism=Escherichia coli, GI1789828, Length=228, Percent_Identity=38.5964912280702, Blast_Score=135, Evalue=1e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR003018
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078 [H]

Pfam domain/function: PF01590 GAF; PF02954 HTH_8; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 84162; Mature: 84162

Theoretical pI: Translated: 8.98; Mature: 8.98

Prosite motif: PS00676 SIGMA54_INTERACT_2 ; PS50045 SIGMA54_INTERACT_4 ; PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEPIITVKEKCRKCYCCVRSCPVKAIKVAKSYTEIIVDRCIGCGNCLSNCPQQAKMVADK
CCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCHHHHHCHHHHHHHHHH
VGVTEKLLSSGEEVIAVLGSSFPAFFHNVTPGQLVAGLRKIGFAEVHEGSYGAELIADDY
HCHHHHHHHCCHHHHHHHHCCCHHHHHCCCHHHHHHHHHHCCCHHCCCCCCCHHHHHHHH
ARITSEKGHPRISSHCPAIVDLIERHYPKLVGNLVPVVSPMVAMGRYLKGTLGQHVRVVY
HHHHCCCCCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEE
ISSCVANKLETQTQETRGAVDIVLTYRELEGIFRSRQIALPALADEPLDGIRPGAGRLFP
HHHHHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCCEEE
IADGTFRAFGIPFDPLDTEIVAACGEVNVMGIINDLAAGRISPRIADLRFCYDGCIGGPG
ECCCCEEEECCCCCCCCHHHHHHCCCCEEHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCC
RNRALTEFYRRNRVIAHFKQEVPCRTVPNSLLEAGRVSFGRSFASKYAKLEAPKANDVRK
CCHHHHHHHHHCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
ILNATNKFTVKDELNCRACGYRTCREYAVAVFQGLAEIEMCLPYNLQQLEEDRGRLIQKY
HHCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
ELARRELEREYGDEFIVGNDRKTLDVLGLIKQVGPTPTTVLIRGESGTGKELTARAIHRY
HHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHH
SKRNDKPLVTVNCTTITDSLLESELFGHKRGAFTGAVADKKGLFEAADGGTIFLDEIGDI
HCCCCCCEEEEEEHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEECCCCEEEECCCCCC
TPKLQAELLRVLDMGEVRPVGGTAAKKVDVRLIAATNKNLEQGVREGWFREDLYYRLNVF
CHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEEECCCCHHHHHHHCCHHHCCEEEEEEE
TITMPPLRSRVESIPILVHHFMDKASTKLNKRMVGIEDRAVKALTKYPWPGNIREMQNVI
EEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCHHHHHHHH
ERAAVLTHDGVIRVENFPLALSEGLEEGFATGLDIHAASFRSEREQHMGKLEKKLIQRYL
HHHHHHCCCCEEEEECCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
TEANGNISRAAKLANIPRRTFYRLLDKYRLRERDVR
HHCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MEPIITVKEKCRKCYCCVRSCPVKAIKVAKSYTEIIVDRCIGCGNCLSNCPQQAKMVADK
CCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCHHHHHCHHHHHHHHHH
VGVTEKLLSSGEEVIAVLGSSFPAFFHNVTPGQLVAGLRKIGFAEVHEGSYGAELIADDY
HCHHHHHHHCCHHHHHHHHCCCHHHHHCCCHHHHHHHHHHCCCHHCCCCCCCHHHHHHHH
ARITSEKGHPRISSHCPAIVDLIERHYPKLVGNLVPVVSPMVAMGRYLKGTLGQHVRVVY
HHHHCCCCCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEE
ISSCVANKLETQTQETRGAVDIVLTYRELEGIFRSRQIALPALADEPLDGIRPGAGRLFP
HHHHHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCCEEE
IADGTFRAFGIPFDPLDTEIVAACGEVNVMGIINDLAAGRISPRIADLRFCYDGCIGGPG
ECCCCEEEECCCCCCCCHHHHHHCCCCEEHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCC
RNRALTEFYRRNRVIAHFKQEVPCRTVPNSLLEAGRVSFGRSFASKYAKLEAPKANDVRK
CCHHHHHHHHHCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
ILNATNKFTVKDELNCRACGYRTCREYAVAVFQGLAEIEMCLPYNLQQLEEDRGRLIQKY
HHCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
ELARRELEREYGDEFIVGNDRKTLDVLGLIKQVGPTPTTVLIRGESGTGKELTARAIHRY
HHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHH
SKRNDKPLVTVNCTTITDSLLESELFGHKRGAFTGAVADKKGLFEAADGGTIFLDEIGDI
HCCCCCCEEEEEEHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEECCCCEEEECCCCCC
TPKLQAELLRVLDMGEVRPVGGTAAKKVDVRLIAATNKNLEQGVREGWFREDLYYRLNVF
CHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEEECCCCHHHHHHHCCHHHCCEEEEEEE
TITMPPLRSRVESIPILVHHFMDKASTKLNKRMVGIEDRAVKALTKYPWPGNIREMQNVI
EEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCHHHHHHHH
ERAAVLTHDGVIRVENFPLALSEGLEEGFATGLDIHAASFRSEREQHMGKLEKKLIQRYL
HHHHHHCCCCEEEEECCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
TEANGNISRAAKLANIPRRTFYRLLDKYRLRERDVR
HHCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2722750 [H]