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The map label for this gene is dnaA

Identifier: 78221229

GI number: 78221229

Start: 261

End: 1613

Strand: Direct

Name: dnaA

Synonym: Gmet_0001

Alternate gene names: 78221229

Gene position: 261-1613 (Clockwise)

Preceding gene: NA

Following gene: 78221230

Centisome position: 0.01

GC content: 58.09

Gene sequence:

>1353_bases
ATGGAAGATGTTTGGCTCCAGGCACAATCCAATCTGGCCAAAGTGCTCACGCACCAGACCTTCACCACCTGGATAGAGCC
GATCAGGTTCGCCGGAGCCCATAAGAATACTCTCCTCCTGGAGGCCCCCAACCAGTTCATCAGGGACCGGGTCAGAGAGA
GCTATCTTCCCATGATCCTCGAATCGGTCCGCTCCCTCACCGACTCCCACTTCCAGGTTGAACTCCAGGTGGCGGCCCGG
CAACAGGAAAAGACCGCGAAAAGCCCACGCAAAAGCCATACAGAGGATGAGCTCGGGCCCGTTGAGTCGGAAAAATGCGC
GCCTGCCGAATTTTCCACCAATCTGAACGCCAAGTATACCTTCGACACCTTTGTCTGCGGCGGGAGCAACCAGTTCGCCC
ATGCAGCGGCCCTGTCGGTGGCCAACAACCCGGCCGGCAAGTACAATCCACTTTTCATCTACGGCGGCGTGGGTCTGGGG
AAAACCCATCTCCTGACGGCCATCGGCAACCAGGTTCTGACCAAGAACCGCAAGGCCCGGGTCTGTTTCTACACCTCCGA
GAAATTCATGAACGAGCTCATCAATTGCCTCCGTTACCAGAAGATGGAGCAGTTCAGGAACAAGTTCCGCAAGATGGACC
TCCTCCTCATCGACGACATCCAGTTCATCGCCGGCAAGGAACGGACCCAGGAAGAGTTTTTCCACACTTTCAACGCCCTC
TACGAGTCACACAAGCAGATCGTGGTCACCTCGGACAAGTTTCCCAAGGACATCCCCGGCCTGGAGGAACGGCTCCGCTC
CCGCTTCGAGTGGGGACTCATCGCCGACATCCAGCCCCCGGACACCGAGACCAAGGTGGCCATCCTCAGCAAGAAGGCCG
ATTCCGACGGCATCCGCCTTCCGGACGACGTGGCGCTCTTTCTTGCCTCCAGCGCCAGCACCAACGTGCGGGAGCTGGAA
GGGATGCTGATCCGCCTCGGCGCCGTGGCGAGCCTCACGGGAAAGAACATCACCCTTGACATGGCCCGGGAGGTTCTGAA
AGACATCATCGTCGACAAGACCAAAGAGGTCACCGTGGAGATGATCCAGAAGTATGTGGCCGACCACTTCAACATCAAGG
TGGCGGAGCTTAAGTCGGACAAGCGCCTCAAAGCGCTGGTGGTGCCACGCCAGATCGCCATTTACCTCTGCCGCGACCTG
ACCAAGGCCTCGTACCCGGATATCGGGGAGAAATTCGGAGGGAAGGACCACTCGACCATCATCCACTCGGTGAAGAAGGT
GGACAAGCTCCTCTCCCAGGATTTCGAGCTGAAAAGCACCATTGAGACCCTGCGCAAGGGGCTGTTGAACTAA

Upstream 100 bases:

>100_bases
GTAGCTTTTCCACTTCCAATACCTCAATTTATCAACAAAAATCCAGTTATTCACACCTGTTGACAACCATGTGTATAACT
GGTCCCCGAGACTACCCTCT

Downstream 100 bases:

>100_bases
AGGGGACAACCCTGTGAATGGAGGGACCCTTTGTGGACAACCTTCCTATCCTTGACCGTCCGCTCCGGGATAACCCGGCA
GCCGTCAACAGGGAAGTACT

Product: chromosomal replication initiation protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 450; Mature: 450

Protein sequence:

>450_residues
MEDVWLQAQSNLAKVLTHQTFTTWIEPIRFAGAHKNTLLLEAPNQFIRDRVRESYLPMILESVRSLTDSHFQVELQVAAR
QQEKTAKSPRKSHTEDELGPVESEKCAPAEFSTNLNAKYTFDTFVCGGSNQFAHAAALSVANNPAGKYNPLFIYGGVGLG
KTHLLTAIGNQVLTKNRKARVCFYTSEKFMNELINCLRYQKMEQFRNKFRKMDLLLIDDIQFIAGKERTQEEFFHTFNAL
YESHKQIVVTSDKFPKDIPGLEERLRSRFEWGLIADIQPPDTETKVAILSKKADSDGIRLPDDVALFLASSASTNVRELE
GMLIRLGAVASLTGKNITLDMAREVLKDIIVDKTKEVTVEMIQKYVADHFNIKVAELKSDKRLKALVVPRQIAIYLCRDL
TKASYPDIGEKFGGKDHSTIIHSVKKVDKLLSQDFELKSTIETLRKGLLN

Sequences:

>Translated_450_residues
MEDVWLQAQSNLAKVLTHQTFTTWIEPIRFAGAHKNTLLLEAPNQFIRDRVRESYLPMILESVRSLTDSHFQVELQVAAR
QQEKTAKSPRKSHTEDELGPVESEKCAPAEFSTNLNAKYTFDTFVCGGSNQFAHAAALSVANNPAGKYNPLFIYGGVGLG
KTHLLTAIGNQVLTKNRKARVCFYTSEKFMNELINCLRYQKMEQFRNKFRKMDLLLIDDIQFIAGKERTQEEFFHTFNAL
YESHKQIVVTSDKFPKDIPGLEERLRSRFEWGLIADIQPPDTETKVAILSKKADSDGIRLPDDVALFLASSASTNVRELE
GMLIRLGAVASLTGKNITLDMAREVLKDIIVDKTKEVTVEMIQKYVADHFNIKVAELKSDKRLKALVVPRQIAIYLCRDL
TKASYPDIGEKFGGKDHSTIIHSVKKVDKLLSQDFELKSTIETLRKGLLN
>Mature_450_residues
MEDVWLQAQSNLAKVLTHQTFTTWIEPIRFAGAHKNTLLLEAPNQFIRDRVRESYLPMILESVRSLTDSHFQVELQVAAR
QQEKTAKSPRKSHTEDELGPVESEKCAPAEFSTNLNAKYTFDTFVCGGSNQFAHAAALSVANNPAGKYNPLFIYGGVGLG
KTHLLTAIGNQVLTKNRKARVCFYTSEKFMNELINCLRYQKMEQFRNKFRKMDLLLIDDIQFIAGKERTQEEFFHTFNAL
YESHKQIVVTSDKFPKDIPGLEERLRSRFEWGLIADIQPPDTETKVAILSKKADSDGIRLPDDVALFLASSASTNVRELE
GMLIRLGAVASLTGKNITLDMAREVLKDIIVDKTKEVTVEMIQKYVADHFNIKVAELKSDKRLKALVVPRQIAIYLCRDL
TKASYPDIGEKFGGKDHSTIIHSVKKVDKLLSQDFELKSTIETLRKGLLN

Specific function: Plays an important role in the initiation and regulation of chromosomal replication. Binds to the origin of replication; it binds specifically double-stranded DNA at a 9 bp consensus (dnaA box):5'-TTATC[CA]A[CA]A-3'. DnaA binds to ATP and to acidic phosph

COG id: COG0593

COG function: function code L; ATPase involved in DNA replication initiation

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dnaA family

Homologues:

Organism=Escherichia coli, GI2367267, Length=465, Percent_Identity=43.8709677419355, Blast_Score=404, Evalue=1e-114,
Organism=Escherichia coli, GI226510964, Length=247, Percent_Identity=27.9352226720648, Blast_Score=76, Evalue=5e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DNAA_GEOMG (Q39ZS3)

Other databases:

- EMBL:   CP000148
- RefSeq:   YP_382976.1
- ProteinModelPortal:   Q39ZS3
- STRING:   Q39ZS3
- GeneID:   3739696
- GenomeReviews:   CP000148_GR
- KEGG:   gme:Gmet_0001
- NMPDR:   fig|269799.3.peg.65
- eggNOG:   COG0593
- HOGENOM:   HBG695666
- OMA:   CHTLNAL
- PhylomeDB:   Q39ZS3
- ProtClustDB:   PRK00149
- BioCyc:   GMET269799:GMET_0001-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00377
- InterPro:   IPR003593
- InterPro:   IPR001957
- InterPro:   IPR020591
- InterPro:   IPR018312
- InterPro:   IPR013159
- InterPro:   IPR013317
- InterPro:   IPR010921
- Gene3D:   G3DSA:1.10.1750.10
- PRINTS:   PR00051
- SMART:   SM00382
- SMART:   SM00760
- TIGRFAMs:   TIGR00362

Pfam domain/function: PF00308 Bac_DnaA; PF08299 Bac_DnaA_C; SSF48295 Trp_repress_rep

EC number: NA

Molecular weight: Translated: 50943; Mature: 50943

Theoretical pI: Translated: 8.85; Mature: 8.85

Prosite motif: PS01008 DNAA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEDVWLQAQSNLAKVLTHQTFTTWIEPIRFAGAHKNTLLLEAPNQFIRDRVRESYLPMIL
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHHHHHH
ESVRSLTDSHFQVELQVAARQQEKTAKSPRKSHTEDELGPVESEKCAPAEFSTNLNAKYT
HHHHHHCCCCEEEEEHHHHHHHHHHHCCCCCCCCCHHCCCCCCCCCCCCHHCCCCCCEEE
FDTFVCGGSNQFAHAAALSVANNPAGKYNPLFIYGGVGLGKTHLLTAIGNQVLTKNRKAR
EEEEEECCCCCHHHHHHHHHCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHCCCCEE
VCFYTSEKFMNELINCLRYQKMEQFRNKFRKMDLLLIDDIQFIAGKERTQEEFFHTFNAL
EEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCHHHHHCCCHHHHHHHHHHHHH
YESHKQIVVTSDKFPKDIPGLEERLRSRFEWGLIADIQPPDTETKVAILSKKADSDGIRL
HHCCCEEEEECCCCCCCCCCHHHHHHHHCCCCEEECCCCCCCCCEEEEEECCCCCCCCCC
PDDVALFLASSASTNVRELEGMLIRLGAVASLTGKNITLDMAREVLKDIIVDKTKEVTVE
CHHHHHHEECCCCCHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHCCHHHHHHH
MIQKYVADHFNIKVAELKSDKRLKALVVPRQIAIYLCRDLTKASYPDIGEKFGGKDHSTI
HHHHHHHHHCCEEEEECCCCCCCEEEECHHHHHHHHHHHHHHCCCCCHHHHHCCCCHHHH
IHSVKKVDKLLSQDFELKSTIETLRKGLLN
HHHHHHHHHHHHCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MEDVWLQAQSNLAKVLTHQTFTTWIEPIRFAGAHKNTLLLEAPNQFIRDRVRESYLPMIL
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHHHHHH
ESVRSLTDSHFQVELQVAARQQEKTAKSPRKSHTEDELGPVESEKCAPAEFSTNLNAKYT
HHHHHHCCCCEEEEEHHHHHHHHHHHCCCCCCCCCHHCCCCCCCCCCCCHHCCCCCCEEE
FDTFVCGGSNQFAHAAALSVANNPAGKYNPLFIYGGVGLGKTHLLTAIGNQVLTKNRKAR
EEEEEECCCCCHHHHHHHHHCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHCCCCEE
VCFYTSEKFMNELINCLRYQKMEQFRNKFRKMDLLLIDDIQFIAGKERTQEEFFHTFNAL
EEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCHHHHHCCCHHHHHHHHHHHHH
YESHKQIVVTSDKFPKDIPGLEERLRSRFEWGLIADIQPPDTETKVAILSKKADSDGIRL
HHCCCEEEEECCCCCCCCCCHHHHHHHHCCCCEEECCCCCCCCCEEEEEECCCCCCCCCC
PDDVALFLASSASTNVRELEGMLIRLGAVASLTGKNITLDMAREVLKDIIVDKTKEVTVE
CHHHHHHEECCCCCHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHCCHHHHHHH
MIQKYVADHFNIKVAELKSDKRLKALVVPRQIAIYLCRDLTKASYPDIGEKFGGKDHSTI
HHHHHHHHHCCEEEEECCCCCCCEEEECHHHHHHHHHHHHHHCCCCCHHHHHCCCCHHHH
IHSVKKVDKLLSQDFELKSTIETLRKGLLN
HHHHHHHHHHHHCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA