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The map label for this gene is pleD [H]

Identifier: 304570568

GI number: 304570568

Start: 849171

End: 850139

Strand: Reverse

Name: pleD [H]

Synonym: NA

Alternate gene names: 304570568

Gene position: 850139-849171 (Counterclockwise)

Preceding gene: 78221994

Following gene: 78221992

Centisome position: 21.27

GC content: 68.11

Gene sequence:

>969_bases
GTGAGGAGCGACATGACCCACGACTCCCTCTTCAGCACCGCCGAACTGGAAACGGTTCCCCTTTTCCGCTTCGTCCCCCT
GGAGTGCATCGAGGGAATCTTGGCCCACTGCACGGCCCAGGAACTCCCCCGGGGCGAGCGGTTCGAACCTGACGGCCCCC
TCGACCGCAGTCTCTCGGTGCTCCTGTCGGGGCGCCTTGCCCTCCACTATGACGTTCGGGAGACCAATGATCCCCTCTTT
CTGGAACCGGGGGATATCATCGGCGAGACGTTCTCCGCGGGAGAACCGGGCGTCCCGTGCCTGCTGGTGGCTGCCGAGCC
CTGCCGCGTCCTCGTCATGGAGGAGGACCTCATCTGGTCCCTGGCCCAGGCATCCCACGCCGCCGCCTGCAACCTCCTCG
GCATCCTCCTGCGGCGGCGCCACGACCCGAGCCGCGAGAGCTTTGCCGGAACGGCGCGCGAATACGCCCTCCACGACCAG
AGCATCGTCGACCCCCTTACCGGTTTCCAGACCCGCCGCTGGTTGGAAGGGGTCCTGGAGCGCCAGGCAGGCCGCGCCGC
CTCCAGCGGTAAACCCCTCTCCCTCATGATGATCGACATCGACCACTTCCGCGCCTTCAACGAAGAGCACGGCCGCATCG
GCGGAGACCGGGCGCTCCATGCCGTGTCCAAGGCCCTGCGCAACTACCTCCGTCCCACCGAGGTGGTGGCCCGCTACGGG
GGGGACACCTTCGCCATCCTCCTCCCCGAAACCGGAGTCGCCACGGCCCGGATGATCGCCGAACGCCTTCGCCAGCGGCT
GACCAACACCGTCATCGACATCCATGACGGCCGCATGCTTCCCCCCCTCACCATATCGGTGGGGGTTGCCGAAGCCGCCG
TCGGCTCGGACAGCCTCACCCTGCTCAGGGGCGCCCTTGAGGCCCTCGACGCCGCCAAGGCCTCGGGGGGGAACGCCGTT
TCCGGCTGA

Upstream 100 bases:
NA

Downstream 100 bases:
NA

Product: cyclic nucleotide-binding domain-containing protein

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 322; Mature: 322

Protein sequence:
NA

Sequences:

>Translated_322_residues
MRSDMTHDSLFSTAELETVPLFRFVPLECIEGILAHCTAQELPRGERFEPDGPLDRSLSVLLSGRLALHYDVRETNDPLF
LEPGDIIGETFSAGEPGVPCLLVAAEPCRVLVMEEDLIWSLAQASHAAACNLLGILLRRRHDPSRESFAGTAREYALHDQ
SIVDPLTGFQTRRWLEGVLERQAGRAASSGKPLSLMMIDIDHFRAFNEEHGRIGGDRALHAVSKALRNYLRPTEVVARYG
GDTFAILLPETGVATARMIAERLRQRLTNTVIDIHDGRMLPPLTISVGVAEAAVGSDSLTLLRGALEALDAAKASGGNAV
SG
>Mature_322_residues
MRSDMTHDSLFSTAELETVPLFRFVPLECIEGILAHCTAQELPRGERFEPDGPLDRSLSVLLSGRLALHYDVRETNDPLF
LEPGDIIGETFSAGEPGVPCLLVAAEPCRVLVMEEDLIWSLAQASHAAACNLLGILLRRRHDPSRESFAGTAREYALHDQ
SIVDPLTGFQTRRWLEGVLERQAGRAASSGKPLSLMMIDIDHFRAFNEEHGRIGGDRALHAVSKALRNYLRPTEVVARYG
GDTFAILLPETGVATARMIAERLRQRLTNTVIDIHDGRMLPPLTISVGVAEAAVGSDSLTLLRGALEALDAAKASGGNAV
SG

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI145693134, Length=158, Percent_Identity=36.0759493670886, Blast_Score=94, Evalue=9e-21,
Organism=Escherichia coli, GI87081881, Length=181, Percent_Identity=31.4917127071823, Blast_Score=78, Evalue=8e-16,
Organism=Escherichia coli, GI1787262, Length=160, Percent_Identity=31.25, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1788085, Length=160, Percent_Identity=30, Blast_Score=75, Evalue=8e-15,
Organism=Escherichia coli, GI1786584, Length=170, Percent_Identity=30.5882352941176, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1788381, Length=100, Percent_Identity=36, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI1787816, Length=151, Percent_Identity=30.4635761589404, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI87081974, Length=108, Percent_Identity=29.6296296296296, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI87082007, Length=131, Percent_Identity=32.0610687022901, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI1787541, Length=154, Percent_Identity=29.2207792207792, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 34994; Mature: 34994

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSDMTHDSLFSTAELETVPLFRFVPLECIEGILAHCTAQELPRGERFEPDGPLDRSLSV
CCCCCCHHHHHHHCCCCCCCHHHHCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHH
LLSGRLALHYDVRETNDPLFLEPGDIIGETFSAGEPGVPCLLVAAEPCRVLVMEEDLIWS
HHCCCEEEEEECCCCCCCEEECCCHHHCCCCCCCCCCCCEEEEECCCCEEEEECHHHHHH
LAQASHAAACNLLGILLRRRHDPSRESFAGTAREYALHDQSIVDPLTGFQTRRWLEGVLE
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
RQAGRAASSGKPLSLMMIDIDHFRAFNEEHGRIGGDRALHAVSKALRNYLRPTEVVARYG
HHHCCCCCCCCCEEEEEEEHHHHHHHCHHHCCCCHHHHHHHHHHHHHHHCCHHHHHHHCC
GDTFAILLPETGVATARMIAERLRQRLTNTVIDIHDGRMLPPLTISVGVAEAAVGSDSLT
CCEEEEEECCCCHHHHHHHHHHHHHHHHHHEEEECCCCCCCCEEEECCHHHHHCCCCHHH
LLRGALEALDAAKASGGNAVSG
HHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MRSDMTHDSLFSTAELETVPLFRFVPLECIEGILAHCTAQELPRGERFEPDGPLDRSLSV
CCCCCCHHHHHHHCCCCCCCHHHHCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHH
LLSGRLALHYDVRETNDPLFLEPGDIIGETFSAGEPGVPCLLVAAEPCRVLVMEEDLIWS
HHCCCEEEEEECCCCCCCEEECCCHHHCCCCCCCCCCCCEEEEECCCCEEEEECHHHHHH
LAQASHAAACNLLGILLRRRHDPSRESFAGTAREYALHDQSIVDPLTGFQTRRWLEGVLE
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
RQAGRAASSGKPLSLMMIDIDHFRAFNEEHGRIGGDRALHAVSKALRNYLRPTEVVARYG
HHHCCCCCCCCCEEEEEEEHHHHHHHCHHHCCCCHHHHHHHHHHHHHHHCCHHHHHHHCC
GDTFAILLPETGVATARMIAERLRQRLTNTVIDIHDGRMLPPLTISVGVAEAAVGSDSLT
CCEEEEEECCCCHHHHHHHHHHHHHHHHHHEEEECCCCCCCCEEEECCHHHHHCCCCHHH
LLRGALEALDAAKASGGNAVSG
HHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA