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The map label for this gene is ygcA [C]

Identifier: 78189981

GI number: 78189981

Start: 2566954

End: 2568387

Strand: Reverse

Name: ygcA [C]

Synonym: Cag_2028

Alternate gene names: 78189981

Gene position: 2568387-2566954 (Counterclockwise)

Preceding gene: 78189982

Following gene: 78189980

Centisome position: 99.86

GC content: 47.42

Gene sequence:

>1434_bases
ATGATGGCAGATATGCAGTACCGTAAGGGCGATGTTATTTCGCTACAAGTGATTGATCTTGCTGAAAAAGATAGTGCCGT
TGGACGGCTTGAAAGTGGCATAACGGTTATGCTTTCAGGGATGGTAGCAATTGGTGACCATGTTTCAGCTCGCATCACCA
AAGTGCGTCAACGCTATCTTGAGGCTGCAACGCTTGACATTTTAGAACCCTCACCCGAACGCACAACGCCTGTTTGCTCT
TACTTTGGCGTGTGCGGTGGTTGCAAACTGATGCACGTTTGTAATGAAGCGCAGTTACGCTACAAGCAAAAAAAGGTAAG
CGACGCTTTAAAGCACCTTGGTGATTTTGTAGAGCCTCCTGTTGCCTCTGTACTTGCAGGCACATCGCCACTCCATTACC
GAAACAAAATGGAGTTTTCCTTTGCGGCGAAACGCTATCTCATGCCCGAAGAGCTTTCGCTTACGGAGCTTTCGCGCTCA
AAAGAGTTTGCACTTGGCTTCCACACGCCCGGTAATTTTGAAAAGGTGATTGATATTGACTACTGCTACCTTGCTCGCAA
TGAGATGAATCAGGTGCTGGAACTTACTCGTCGTTTTGCGCTGCAACATGCTCTTCCTCCTTATTCCGTAAAAACGCATA
CGGGCTTTTTGCGCAACCTTGTGGTACGTTTTAGTGAGCATACACAAGAGCTTATGGTGAACCTTGTAACCTCGTGGCAC
GATGCCGAGGTGATGGCACGCTATAGCGCTATGCTGCATCATGCTATGCCTGAGCAAAAAATGACGGTGGTCAATAACAT
TACCAGCCGAAAAAATGGGGTTGCCACAGGTGAAGAGGAGGTGGTGATAAGCGGAGAGGGATTTATTACCGAACGTTTAT
GTGGACTTGATTTTCGCATTTCAGCAAACTCGTTTTTTCAAACCAACTCCGCACAAGCGGAAGTGCTTTACCAAACGCTG
CTTGCGGTTGCTGATTTACAGCCAACCGATACGGTGTATGACCTCTATTGCGGTACCGGCACGATTACGCTGTGCATGGC
AGCACACTGCCACCAAGTGATTGGCTTAGAGGTGGTTGAAAGTGCTATTCGCGATGCGCAAAACAATGCACTCCGTAACG
GCATTACGAACGCTCATTTTTTATTAGCCGATTTAAAAGATTTCCATACCCTGCTTCCATTACTTGAAGAGCATGGCAAG
CCTCGTGTGATTGTAACCGATCCCCCTCGCGCAGGAATGCACCCAAAAGCGTTAGCAACAATGGTGCAGCTTCAACCTGA
GCGCATTATTTATGTGAGTTGTAATCCAGCAAGCCTTGCTCGTGATGGCAAGGAACTCGCAGCACAAGGTTATTCGTTAC
GTTCAGTGCAGCCGGTTGAAATGTTTCCACAAACCAATCACATTGAGAGCGTTGGTTGTTTTGTGAAGGTGTAA

Upstream 100 bases:

>100_bases
TATGCCGTTACGAACCACAACGTCGCCCCCATCTCAAAACGATGGCTGATGGCTTCTAACAATTCCTTTTTGACTCCTCT
CTTCACCTCTTTTTTTCTCT

Downstream 100 bases:

>100_bases
GGCAATTCGGTAATAGAGAATGGTTTGAAACAAGAAAATAATTCCATGAAAACACTTTACCTTATTCGGCACGCAAAAGC
CAGCAGTGGTAACAACTTTG

Product: 23S rRNA methyltransferase/RumA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 477; Mature: 477

Protein sequence:

>477_residues
MMADMQYRKGDVISLQVIDLAEKDSAVGRLESGITVMLSGMVAIGDHVSARITKVRQRYLEAATLDILEPSPERTTPVCS
YFGVCGGCKLMHVCNEAQLRYKQKKVSDALKHLGDFVEPPVASVLAGTSPLHYRNKMEFSFAAKRYLMPEELSLTELSRS
KEFALGFHTPGNFEKVIDIDYCYLARNEMNQVLELTRRFALQHALPPYSVKTHTGFLRNLVVRFSEHTQELMVNLVTSWH
DAEVMARYSAMLHHAMPEQKMTVVNNITSRKNGVATGEEEVVISGEGFITERLCGLDFRISANSFFQTNSAQAEVLYQTL
LAVADLQPTDTVYDLYCGTGTITLCMAAHCHQVIGLEVVESAIRDAQNNALRNGITNAHFLLADLKDFHTLLPLLEEHGK
PRVIVTDPPRAGMHPKALATMVQLQPERIIYVSCNPASLARDGKELAAQGYSLRSVQPVEMFPQTNHIESVGCFVKV

Sequences:

>Translated_477_residues
MMADMQYRKGDVISLQVIDLAEKDSAVGRLESGITVMLSGMVAIGDHVSARITKVRQRYLEAATLDILEPSPERTTPVCS
YFGVCGGCKLMHVCNEAQLRYKQKKVSDALKHLGDFVEPPVASVLAGTSPLHYRNKMEFSFAAKRYLMPEELSLTELSRS
KEFALGFHTPGNFEKVIDIDYCYLARNEMNQVLELTRRFALQHALPPYSVKTHTGFLRNLVVRFSEHTQELMVNLVTSWH
DAEVMARYSAMLHHAMPEQKMTVVNNITSRKNGVATGEEEVVISGEGFITERLCGLDFRISANSFFQTNSAQAEVLYQTL
LAVADLQPTDTVYDLYCGTGTITLCMAAHCHQVIGLEVVESAIRDAQNNALRNGITNAHFLLADLKDFHTLLPLLEEHGK
PRVIVTDPPRAGMHPKALATMVQLQPERIIYVSCNPASLARDGKELAAQGYSLRSVQPVEMFPQTNHIESVGCFVKV
>Mature_477_residues
MMADMQYRKGDVISLQVIDLAEKDSAVGRLESGITVMLSGMVAIGDHVSARITKVRQRYLEAATLDILEPSPERTTPVCS
YFGVCGGCKLMHVCNEAQLRYKQKKVSDALKHLGDFVEPPVASVLAGTSPLHYRNKMEFSFAAKRYLMPEELSLTELSRS
KEFALGFHTPGNFEKVIDIDYCYLARNEMNQVLELTRRFALQHALPPYSVKTHTGFLRNLVVRFSEHTQELMVNLVTSWH
DAEVMARYSAMLHHAMPEQKMTVVNNITSRKNGVATGEEEVVISGEGFITERLCGLDFRISANSFFQTNSAQAEVLYQTL
LAVADLQPTDTVYDLYCGTGTITLCMAAHCHQVIGLEVVESAIRDAQNNALRNGITNAHFLLADLKDFHTLLPLLEEHGK
PRVIVTDPPRAGMHPKALATMVQLQPERIIYVSCNPASLARDGKELAAQGYSLRSVQPVEMFPQTNHIESVGCFVKV

Specific function: Could Be A 23s rRNA (Uracil-5-)-Methyltransferase. [C]

COG id: COG2265

COG function: function code J; SAM-dependent methyltransferases related to tRNA (uracil-5-)-methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI34222389, Length=377, Percent_Identity=28.3819628647215, Blast_Score=121, Evalue=1e-27,
Organism=Homo sapiens, GI51173878, Length=377, Percent_Identity=28.3819628647215, Blast_Score=121, Evalue=1e-27,
Organism=Homo sapiens, GI269784680, Length=376, Percent_Identity=27.3936170212766, Blast_Score=117, Evalue=3e-26,
Organism=Homo sapiens, GI269784676, Length=376, Percent_Identity=27.3936170212766, Blast_Score=117, Evalue=3e-26,
Organism=Homo sapiens, GI20149688, Length=376, Percent_Identity=27.3936170212766, Blast_Score=117, Evalue=3e-26,
Organism=Homo sapiens, GI269784678, Length=376, Percent_Identity=27.3936170212766, Blast_Score=116, Evalue=4e-26,
Organism=Escherichia coli, GI1789148, Length=471, Percent_Identity=29.0870488322718, Blast_Score=173, Evalue=2e-44,
Organism=Escherichia coli, GI1787083, Length=398, Percent_Identity=27.8894472361809, Blast_Score=126, Evalue=4e-30,
Organism=Escherichia coli, GI1790403, Length=198, Percent_Identity=28.7878787878788, Blast_Score=79, Evalue=8e-16,
Organism=Caenorhabditis elegans, GI71997438, Length=170, Percent_Identity=34.7058823529412, Blast_Score=89, Evalue=4e-18,
Organism=Saccharomyces cerevisiae, GI6322909, Length=450, Percent_Identity=23.3333333333333, Blast_Score=101, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24666579, Length=449, Percent_Identity=30.9576837416481, Blast_Score=157, Evalue=2e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016027
- InterPro:   IPR001566
- InterPro:   IPR002792
- InterPro:   IPR010280 [H]

Pfam domain/function: PF01938 TRAM; PF05958 tRNA_U5-meth_tr [H]

EC number: NA

Molecular weight: Translated: 53106; Mature: 53106

Theoretical pI: Translated: 6.69; Mature: 6.69

Prosite motif: PS50926 TRAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMADMQYRKGDVISLQVIDLAEKDSAVGRLESGITVMLSGMVAIGDHVSARITKVRQRYL
CCCCCCCCCCCEEEEEEEECCCCCHHHHHHHCCHHEEHHHHHHHCCHHHHHHHHHHHHHH
EAATLDILEPSPERTTPVCSYFGVCGGCKLMHVCNEAQLRYKQKKVSDALKHLGDFVEPP
HHHHHHCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
VASVLAGTSPLHYRNKMEFSFAAKRYLMPEELSLTELSRSKEFALGFHTPGNFEKVIDID
HHHHHCCCCCCCCCCCCCHHHHHHHCCCCCCCCHHHHHCCCCEEEEECCCCCCCEEEECH
YCYLARNEMNQVLELTRRFALQHALPPYSVKTHTGFLRNLVVRFSEHTQELMVNLVTSWH
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
DAEVMARYSAMLHHAMPEQKMTVVNNITSRKNGVATGEEEVVISGEGFITERLCGLDFRI
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCEEECCCCCHHHHHCCCEEEE
SANSFFQTNSAQAEVLYQTLLAVADLQPTDTVYDLYCGTGTITLCMAAHCHQVIGLEVVE
CCCCEEECCCHHHHHHHHHHHHHHCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHH
SAIRDAQNNALRNGITNAHFLLADLKDFHTLLPLLEEHGKPRVIVTDPPRAGMHPKALAT
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHH
MVQLQPERIIYVSCNPASLARDGKELAAQGYSLRSVQPVEMFPQTNHIESVGCFVKV
HHHCCCCEEEEEECCHHHHHHCCHHHHHCCCCCCCCCCHHHCCCCCCCCCCCCEEEC
>Mature Secondary Structure
MMADMQYRKGDVISLQVIDLAEKDSAVGRLESGITVMLSGMVAIGDHVSARITKVRQRYL
CCCCCCCCCCCEEEEEEEECCCCCHHHHHHHCCHHEEHHHHHHHCCHHHHHHHHHHHHHH
EAATLDILEPSPERTTPVCSYFGVCGGCKLMHVCNEAQLRYKQKKVSDALKHLGDFVEPP
HHHHHHCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
VASVLAGTSPLHYRNKMEFSFAAKRYLMPEELSLTELSRSKEFALGFHTPGNFEKVIDID
HHHHHCCCCCCCCCCCCCHHHHHHHCCCCCCCCHHHHHCCCCEEEEECCCCCCCEEEECH
YCYLARNEMNQVLELTRRFALQHALPPYSVKTHTGFLRNLVVRFSEHTQELMVNLVTSWH
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
DAEVMARYSAMLHHAMPEQKMTVVNNITSRKNGVATGEEEVVISGEGFITERLCGLDFRI
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCEEECCCCCHHHHHCCCEEEE
SANSFFQTNSAQAEVLYQTLLAVADLQPTDTVYDLYCGTGTITLCMAAHCHQVIGLEVVE
CCCCEEECCCHHHHHHHHHHHHHHCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHH
SAIRDAQNNALRNGITNAHFLLADLKDFHTLLPLLEEHGKPRVIVTDPPRAGMHPKALAT
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHH
MVQLQPERIIYVSCNPASLARDGKELAAQGYSLRSVQPVEMFPQTNHIESVGCFVKV
HHHCCCCEEEEEECCHHHHHHCCHHHHHCCCCCCCCCCHHHCCCCCCCCCCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12093901 [H]