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The map label for this gene is fhlA [H]

Identifier: 78189953

GI number: 78189953

Start: 2535074

End: 2537176

Strand: Reverse

Name: fhlA [H]

Synonym: Cag_2000

Alternate gene names: 78189953

Gene position: 2537176-2535074 (Counterclockwise)

Preceding gene: 78189954

Following gene: 78189952

Centisome position: 98.64

GC content: 50.26

Gene sequence:

>2103_bases
ATGCCCATCACCCACTCTTTGCACGAGCTGCATCGGCTGATGCGAACCATCAGCAACGCCATTGGCTCTCTTCACGATCC
TCAAGAGCTATTCCATACCATTATAACTCAGCTCCGCCTCTTTTTTGCTTTCGATTCTGCTGCCATTATTACCCTCGACA
ACAATCAGCGCCATTGTTCTCTCTTTTTTGAAATGCTGCGTTTTCAATTGCCTGAGGGTTTTGTGCGTTATCAACGCCCT
GTGGCAGGCTCGTGGATTGAGCCGCATGTGGAGGCGCAAAAGGTTGCCGTTATCCGTTTGCGCGATTCACTTGAGCATTT
CCCCGTTGATGTTGAGTTACGCCAACATTTGCTTGAGCTTGGATTAAAGCAAGCAGTGCTTTCGCCTTTGCGGGCAGGTG
GCAAGGTGATTGGTTTTTTGACCTTTGTTTTTCAAGAAGAGCAGGAGTGGCTTGATGAGGAGTGTGAGCTCTTAGCTACC
GTTTCCACGCCTATTGCCGTTGCCGTGAGCAATGCGTTAGCTTACGAGGAACTCCGTAAGCGGGAGGCGCAAACCGCTAT
GCAGCTTGCCGTTAACAATGCGTTGCTTACCATTCGTGAGCGCAATAGCATGATGCTTGCGGTGTGTGAGCAGGTGCAAA
AGTTGCTGCCTTGTACCTTTCTTGGCATTAGGGTGATGGATAAGGAGGGCAACTATCGCTTGTACGATAACTTTCGTCTT
CAGCCTGATGGAGCCTTTGCGCCTGTAACTGCGGCACTTGATATGGAGAATTACGACTCCATTGCCCGTGCAAGTTTTGA
TTTAGCCATCATTGGTGGTTACTACGGCGGCAACAGCTTTCTTGAGCTTTGCGCTCGCTTTCCAATTTTAGAGCATGTTC
GCCAAAAATATGGCATTTGCTCATTCTTGAGCCTTCCGTTATGGGAGCTGCCAGCCAGCCGTGCTGGGCTTATTATTTCT
ACCACTATGCGCCCTTTTGATGGGCACGATCAAGCAACGGCGAATCTTATTGTGCCGCAACTTTCGCTTGCCTTGCAAAA
CTATCTTGCCTTTGAAGAAATTAACGAGTTGCGCCATAAGCTTGAGGGTGAACGAAGCTGCCTTATTGAAGAGATTACGG
CAACGCATCATGTTGGGGAAATTATAGGGAGCAGCTCTGCGTTAACTGCCGTGCTCTATCGCCTCCAGCAAGTTGCGCCC
ACCGATGCCACCGTACTTATTCAAGGCGAAACGGGCACAGGTAAAGAGCTTTTTGCGCGTGCCTTGCACAACCTTTCAGC
CCGAAGCAAACGCGCTCTTATTAAGGTGAACTGTGCCACCCTTCCCGCCTCGCTTATTGAGTCGGAACTTTTTGGACATG
AAAAAGGGAGCTTTACGGGAGCCACAGAACGGCGTATTGGTAAATTTGAGTTAGCCGAAGGGGGCACTATTTTTTTGGAT
GAAATTGGGGAGCTGCCACTTGAGTTGCAGGCAAAGCTGCTACGCGTGTTGCAAGAGCGTGAATTTGAACGGCTTGGTGG
ACGCCACGTTATTCGTGCAAATGTGCGCGTTATTGCTGCTACCAATCGCAATGTAGAGCAAGAGGTTGCTTCAGGGCGAT
TCCGCGAAGACCTCTATTTTCGCCTTAACGTGGTGCCACTTCACGTTCCACCGCTTCGTGAACGGCGCGATGATATTGCT
CTGCTTGCCAACCACTTTGCGAACCGCTACGCCCGTGAGTTTAGCAAGCCAAACCGTCCCATCCGCCAAAACGATATGCA
ACGCTTGCTCGGGCGCGAATGGAAAGGCAACATTCGTGAACTTGCCCACATGGTTGAACAAGCCGTTATTCTCTCGGAAG
GCTCGACGCTTGATTTCGCAACCATTCTTGCTCCCCAACAAACACTCAATGCTCCTACAAACCGTGCTATCTCCAGCCTC
CGCACTATGCGCGCCTTTGAGGAGGAAATGATTGCGATGGAAAAGCAGCTTATTCTTGATACTCTTGAAGCAACGGGTGG
ACGGGTAAGTGGCTCTGGAGGTGCTGCCGAACGGCTTGCTATGCACCCCAAAACGCTTTATACCCGTATTGCAACGTTAG
GATTAAGCAAGCGGTATGGATAG

Upstream 100 bases:

>100_bases
ACATCATCCATTCCCCCACTTTTCTTGAATCACGATTCCACAAATTTCAGGAAAAGCTGCACATTGCAGGGAAACATATT
CCCCTTATTTGCTTGCAACC

Downstream 100 bases:

>100_bases
GTGGAACATGCTTCTTAGAGAGCATTGCACTTCCCCCAACAATCTACATTTTTGCTGTTTTTTAGCTTATAACGCCCATT
AAGCTTCCTTTTCAAGGGAA

Product: Fis family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 700; Mature: 699

Protein sequence:

>700_residues
MPITHSLHELHRLMRTISNAIGSLHDPQELFHTIITQLRLFFAFDSAAIITLDNNQRHCSLFFEMLRFQLPEGFVRYQRP
VAGSWIEPHVEAQKVAVIRLRDSLEHFPVDVELRQHLLELGLKQAVLSPLRAGGKVIGFLTFVFQEEQEWLDEECELLAT
VSTPIAVAVSNALAYEELRKREAQTAMQLAVNNALLTIRERNSMMLAVCEQVQKLLPCTFLGIRVMDKEGNYRLYDNFRL
QPDGAFAPVTAALDMENYDSIARASFDLAIIGGYYGGNSFLELCARFPILEHVRQKYGICSFLSLPLWELPASRAGLIIS
TTMRPFDGHDQATANLIVPQLSLALQNYLAFEEINELRHKLEGERSCLIEEITATHHVGEIIGSSSALTAVLYRLQQVAP
TDATVLIQGETGTGKELFARALHNLSARSKRALIKVNCATLPASLIESELFGHEKGSFTGATERRIGKFELAEGGTIFLD
EIGELPLELQAKLLRVLQEREFERLGGRHVIRANVRVIAATNRNVEQEVASGRFREDLYFRLNVVPLHVPPLRERRDDIA
LLANHFANRYAREFSKPNRPIRQNDMQRLLGREWKGNIRELAHMVEQAVILSEGSTLDFATILAPQQTLNAPTNRAISSL
RTMRAFEEEMIAMEKQLILDTLEATGGRVSGSGGAAERLAMHPKTLYTRIATLGLSKRYG

Sequences:

>Translated_700_residues
MPITHSLHELHRLMRTISNAIGSLHDPQELFHTIITQLRLFFAFDSAAIITLDNNQRHCSLFFEMLRFQLPEGFVRYQRP
VAGSWIEPHVEAQKVAVIRLRDSLEHFPVDVELRQHLLELGLKQAVLSPLRAGGKVIGFLTFVFQEEQEWLDEECELLAT
VSTPIAVAVSNALAYEELRKREAQTAMQLAVNNALLTIRERNSMMLAVCEQVQKLLPCTFLGIRVMDKEGNYRLYDNFRL
QPDGAFAPVTAALDMENYDSIARASFDLAIIGGYYGGNSFLELCARFPILEHVRQKYGICSFLSLPLWELPASRAGLIIS
TTMRPFDGHDQATANLIVPQLSLALQNYLAFEEINELRHKLEGERSCLIEEITATHHVGEIIGSSSALTAVLYRLQQVAP
TDATVLIQGETGTGKELFARALHNLSARSKRALIKVNCATLPASLIESELFGHEKGSFTGATERRIGKFELAEGGTIFLD
EIGELPLELQAKLLRVLQEREFERLGGRHVIRANVRVIAATNRNVEQEVASGRFREDLYFRLNVVPLHVPPLRERRDDIA
LLANHFANRYAREFSKPNRPIRQNDMQRLLGREWKGNIRELAHMVEQAVILSEGSTLDFATILAPQQTLNAPTNRAISSL
RTMRAFEEEMIAMEKQLILDTLEATGGRVSGSGGAAERLAMHPKTLYTRIATLGLSKRYG
>Mature_699_residues
PITHSLHELHRLMRTISNAIGSLHDPQELFHTIITQLRLFFAFDSAAIITLDNNQRHCSLFFEMLRFQLPEGFVRYQRPV
AGSWIEPHVEAQKVAVIRLRDSLEHFPVDVELRQHLLELGLKQAVLSPLRAGGKVIGFLTFVFQEEQEWLDEECELLATV
STPIAVAVSNALAYEELRKREAQTAMQLAVNNALLTIRERNSMMLAVCEQVQKLLPCTFLGIRVMDKEGNYRLYDNFRLQ
PDGAFAPVTAALDMENYDSIARASFDLAIIGGYYGGNSFLELCARFPILEHVRQKYGICSFLSLPLWELPASRAGLIIST
TMRPFDGHDQATANLIVPQLSLALQNYLAFEEINELRHKLEGERSCLIEEITATHHVGEIIGSSSALTAVLYRLQQVAPT
DATVLIQGETGTGKELFARALHNLSARSKRALIKVNCATLPASLIESELFGHEKGSFTGATERRIGKFELAEGGTIFLDE
IGELPLELQAKLLRVLQEREFERLGGRHVIRANVRVIAATNRNVEQEVASGRFREDLYFRLNVVPLHVPPLRERRDDIAL
LANHFANRYAREFSKPNRPIRQNDMQRLLGREWKGNIRELAHMVEQAVILSEGSTLDFATILAPQQTLNAPTNRAISSLR
TMRAFEEEMIAMEKQLILDTLEATGGRVSGSGGAAERLAMHPKTLYTRIATLGLSKRYG

Specific function: Required for induction of expression of the formate dehydrogenase H and hydrogenase-3 structural genes [H]

COG id: COG3604

COG function: function code KT; Transcriptional regulator containing GAF, AAA-type ATPase, and DNA binding domains

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1789087, Length=362, Percent_Identity=48.0662983425414, Blast_Score=330, Evalue=2e-91,
Organism=Escherichia coli, GI87082117, Length=367, Percent_Identity=47.4114441416894, Blast_Score=322, Evalue=4e-89,
Organism=Escherichia coli, GI1788550, Length=350, Percent_Identity=45.7142857142857, Blast_Score=277, Evalue=2e-75,
Organism=Escherichia coli, GI1790437, Length=311, Percent_Identity=45.3376205787781, Blast_Score=263, Evalue=3e-71,
Organism=Escherichia coli, GI1788905, Length=243, Percent_Identity=49.3827160493827, Blast_Score=236, Evalue=4e-63,
Organism=Escherichia coli, GI87082152, Length=284, Percent_Identity=46.4788732394366, Blast_Score=232, Evalue=5e-62,
Organism=Escherichia coli, GI1790299, Length=235, Percent_Identity=48.0851063829787, Blast_Score=227, Evalue=2e-60,
Organism=Escherichia coli, GI1789233, Length=322, Percent_Identity=38.1987577639752, Blast_Score=226, Evalue=5e-60,
Organism=Escherichia coli, GI87081872, Length=230, Percent_Identity=46.5217391304348, Blast_Score=203, Evalue=3e-53,
Organism=Escherichia coli, GI1786524, Length=301, Percent_Identity=39.8671096345515, Blast_Score=199, Evalue=5e-52,
Organism=Escherichia coli, GI1787583, Length=247, Percent_Identity=42.5101214574899, Blast_Score=188, Evalue=1e-48,
Organism=Escherichia coli, GI1789828, Length=246, Percent_Identity=37.8048780487805, Blast_Score=137, Evalue=2e-33,
Organism=Escherichia coli, GI87081858, Length=274, Percent_Identity=32.4817518248175, Blast_Score=134, Evalue=3e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR003018
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078 [H]

Pfam domain/function: PF01590 GAF; PF02954 HTH_8; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 78705; Mature: 78574

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPITHSLHELHRLMRTISNAIGSLHDPQELFHTIITQLRLFFAFDSAAIITLDNNQRHCS
CCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHEECCEEEEEECCCCHHHH
LFFEMLRFQLPEGFVRYQRPVAGSWIEPHVEAQKVAVIRLRDSLEHFPVDVELRQHLLEL
HHHHHHHHHCCHHHHHHCCCCCCCCCCCCCCHHHEEEHHHHHHHHHCCCCHHHHHHHHHH
GLKQAVLSPLRAGGKVIGFLTFVFQEEQEWLDEECELLATVSTPIAVAVSNALAYEELRK
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
REAQTAMQLAVNNALLTIRERNSMMLAVCEQVQKLLPCTFLGIRVMDKEGNYRLYDNFRL
HHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHCCHHEECEEEEECCCCEEEEECEEE
QPDGAFAPVTAALDMENYDSIARASFDLAIIGGYYGGNSFLELCARFPILEHVRQKYGIC
CCCCCCCCEEEEECCCCHHHHHHCCCCEEEEECCCCCHHHHHHHHHCHHHHHHHHHHCCH
SFLSLPLWELPASRAGLIISTTMRPFDGHDQATANLIVPQLSLALQNYLAFEEINELRHK
HHHHCCHHHCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LEGERSCLIEEITATHHVGEIIGSSSALTAVLYRLQQVAPTDATVLIQGETGTGKELFAR
HCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHH
ALHNLSARSKRALIKVNCATLPASLIESELFGHEKGSFTGATERRIGKFELAEGGTIFLD
HHHHHHHCCCCEEEEEEHHHCCHHHHHHHHHCCCCCCCCCHHHHCCCCEEECCCCEEEEH
EIGELPLELQAKLLRVLQEREFERLGGRHVIRANVRVIAATNRNVEQEVASGRFREDLYF
HHCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEECEEEEEECCCCHHHHHHCCCCCCCEEE
RLNVVPLHVPPLRERRDDIALLANHFANRYAREFSKPNRPIRQNDMQRLLGREWKGNIRE
EEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCCHHH
LAHMVEQAVILSEGSTLDFATILAPQQTLNAPTNRAISSLRTMRAFEEEMIAMEKQLILD
HHHHHHHHHHHCCCCCCCHHHHCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TLEATGGRVSGSGGAAERLAMHPKTLYTRIATLGLSKRYG
HHHHCCCEECCCCCHHHHHHCCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
PITHSLHELHRLMRTISNAIGSLHDPQELFHTIITQLRLFFAFDSAAIITLDNNQRHCS
CCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHEECCEEEEEECCCCHHHH
LFFEMLRFQLPEGFVRYQRPVAGSWIEPHVEAQKVAVIRLRDSLEHFPVDVELRQHLLEL
HHHHHHHHHCCHHHHHHCCCCCCCCCCCCCCHHHEEEHHHHHHHHHCCCCHHHHHHHHHH
GLKQAVLSPLRAGGKVIGFLTFVFQEEQEWLDEECELLATVSTPIAVAVSNALAYEELRK
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
REAQTAMQLAVNNALLTIRERNSMMLAVCEQVQKLLPCTFLGIRVMDKEGNYRLYDNFRL
HHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHCCHHEECEEEEECCCCEEEEECEEE
QPDGAFAPVTAALDMENYDSIARASFDLAIIGGYYGGNSFLELCARFPILEHVRQKYGIC
CCCCCCCCEEEEECCCCHHHHHHCCCCEEEEECCCCCHHHHHHHHHCHHHHHHHHHHCCH
SFLSLPLWELPASRAGLIISTTMRPFDGHDQATANLIVPQLSLALQNYLAFEEINELRHK
HHHHCCHHHCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LEGERSCLIEEITATHHVGEIIGSSSALTAVLYRLQQVAPTDATVLIQGETGTGKELFAR
HCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHH
ALHNLSARSKRALIKVNCATLPASLIESELFGHEKGSFTGATERRIGKFELAEGGTIFLD
HHHHHHHCCCCEEEEEEHHHCCHHHHHHHHHCCCCCCCCCHHHHCCCCEEECCCCEEEEH
EIGELPLELQAKLLRVLQEREFERLGGRHVIRANVRVIAATNRNVEQEVASGRFREDLYF
HHCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEECEEEEEECCCCHHHHHHCCCCCCCEEE
RLNVVPLHVPPLRERRDDIALLANHFANRYAREFSKPNRPIRQNDMQRLLGREWKGNIRE
EEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCCHHH
LAHMVEQAVILSEGSTLDFATILAPQQTLNAPTNRAISSLRTMRAFEEEMIAMEKQLILD
HHHHHHHHHHHCCCCCCCHHHHCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TLEATGGRVSGSGGAAERLAMHPKTLYTRIATLGLSKRYG
HHHHCCCEECCCCCHHHHHHCCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2280686; 2118503; 9278503 [H]