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The map label for this gene is amt [H]

Identifier: 78189952

GI number: 78189952

Start: 2533520

End: 2534854

Strand: Reverse

Name: amt [H]

Synonym: Cag_1999

Alternate gene names: 78189952

Gene position: 2534854-2533520 (Counterclockwise)

Preceding gene: 78189953

Following gene: 78189951

Centisome position: 98.55

GC content: 48.24

Gene sequence:

>1335_bases
ATGAAGCACAAACACTTAACGGCGCTGCTCATGCTTGGGGCGTTGCTTTTGACGGGAGGCAACGCTTGGGCGGCAGATGA
GGTCGTTACGGATAGTGGCACAACCTCGTGGATGCTTACCTCTACGGCGCTTGTGCTGCTTATGATTCCCGGACTTGCAA
TGTTTTACGGCGGTTTGGTGCGCACCAAAAATGTGCTTGGCACCATGATGCATAGCTTTGGTGCCATGGTCATTATTGGC
GTGCTATGGCCTATGGTTGGCTATGGATTAAGCTTTGGACCTGGCATTCTTGGTGGTTTAGCGGGATGGGACGATAAGTT
CTTTTTCTTGCAAGGTATTGATGATTCTATTATGAGTACACACATTCCCGAATATGTGTTTGCTATGTTCCAAGGCAAAT
TTGCCATTATTACACCAGCGCTTATTGCAGGTGCTTTTGCTGAAAGGGTTAATTTTAAAGGCTATGCAGTGTTCATAGCG
CTATGGTGCATTTTGGTTTATAGCCCAATTTGCCACTGGGTGTGGGCTTCTGACGGCTTCCTTTTTAACCTTGGTCCATC
GGGTGCTATTGATTTTGCGGGTGGTACGGTTGTACACATTTCATCGGGCATTACGGCGTTAGTTGCCGCCTTGTACCTTG
GCAAACGTCGTGGTTATCCCAGCACGGCAATGTCGCCCAACAACTTGGTGATGACGCTTATTGGCGCTGGTTTGCTTTGG
GTTGGATGGTTTGGTTTTAACGCTGGTAGTGCTATTGCCAGCGATCTTGTAACGGCTCGCGCTTTAACGGTTACACAAGT
TGCTGCCGCTTCGGGCGCTTTTGCTTGGATGATTATTGAAATGTTCCATCATGGAAAAGCCACCAGCCTTGGTGTTGCTT
CGGGTATTCTTGCTGGGCTTGTGGCAATTACTCCAGCCGCCGGTGTGGTTCAGCCAATTGGTGCTTTTGCTCTTGGTGCT
CTTGCGGCTGCCATTTGCTACAGTGCAATTCTTTTAAAAAGCAAGCTTGGCTACGACGACAGCCTTGATGCTTTTGGCGT
GCATGGTGTTGGTGGTATTGTTGGCGCTTTAGCTCTTGTTTTCTTTATTCGCCCAAGCTGGTTAGCTGATGCGGCTGCTA
AAACCGCCAACAACAGTTGGACAATGTGGGATCAACTTGGCGTGCAAGCTACAGCCGTTGGCGTTACCATTGCGTATGCA
GCCGTTATTTCCTTTGTACTGCTCTTTATTGTAGAAAAAACTGTAGGCTTGCGCATTTCAGAGGATGATGAAATGTCGGG
GCTTGATCACAGTATGCACGGTGAACAAGGATATGGGCTTATCAACCTTAACTAA

Upstream 100 bases:

>100_bases
TAAAAAAGCTTTTAGAGTTTGTATTGGTTAAAAAAATAACTACATTTTTGTCGTCAGCATTAATTATTAAGGTTTTCTTA
AACAATAACCACTATTTCTT

Downstream 100 bases:

>100_bases
GTAAAGCAATGAAACTGATAACAGCTATTATTCAGCCCGATAGGCTCGACCGTGTGCGTGAAGCGCTTATTCAAGCCGAT
ATCACTCGAATAACTGTAAG

Product: ammonium transporter

Products: NH3 [Cytoplasm] [C]

Alternate protein names: Ammonia transporter [H]

Number of amino acids: Translated: 444; Mature: 444

Protein sequence:

>444_residues
MKHKHLTALLMLGALLLTGGNAWAADEVVTDSGTTSWMLTSTALVLLMIPGLAMFYGGLVRTKNVLGTMMHSFGAMVIIG
VLWPMVGYGLSFGPGILGGLAGWDDKFFFLQGIDDSIMSTHIPEYVFAMFQGKFAIITPALIAGAFAERVNFKGYAVFIA
LWCILVYSPICHWVWASDGFLFNLGPSGAIDFAGGTVVHISSGITALVAALYLGKRRGYPSTAMSPNNLVMTLIGAGLLW
VGWFGFNAGSAIASDLVTARALTVTQVAAASGAFAWMIIEMFHHGKATSLGVASGILAGLVAITPAAGVVQPIGAFALGA
LAAAICYSAILLKSKLGYDDSLDAFGVHGVGGIVGALALVFFIRPSWLADAAAKTANNSWTMWDQLGVQATAVGVTIAYA
AVISFVLLFIVEKTVGLRISEDDEMSGLDHSMHGEQGYGLINLN

Sequences:

>Translated_444_residues
MKHKHLTALLMLGALLLTGGNAWAADEVVTDSGTTSWMLTSTALVLLMIPGLAMFYGGLVRTKNVLGTMMHSFGAMVIIG
VLWPMVGYGLSFGPGILGGLAGWDDKFFFLQGIDDSIMSTHIPEYVFAMFQGKFAIITPALIAGAFAERVNFKGYAVFIA
LWCILVYSPICHWVWASDGFLFNLGPSGAIDFAGGTVVHISSGITALVAALYLGKRRGYPSTAMSPNNLVMTLIGAGLLW
VGWFGFNAGSAIASDLVTARALTVTQVAAASGAFAWMIIEMFHHGKATSLGVASGILAGLVAITPAAGVVQPIGAFALGA
LAAAICYSAILLKSKLGYDDSLDAFGVHGVGGIVGALALVFFIRPSWLADAAAKTANNSWTMWDQLGVQATAVGVTIAYA
AVISFVLLFIVEKTVGLRISEDDEMSGLDHSMHGEQGYGLINLN
>Mature_444_residues
MKHKHLTALLMLGALLLTGGNAWAADEVVTDSGTTSWMLTSTALVLLMIPGLAMFYGGLVRTKNVLGTMMHSFGAMVIIG
VLWPMVGYGLSFGPGILGGLAGWDDKFFFLQGIDDSIMSTHIPEYVFAMFQGKFAIITPALIAGAFAERVNFKGYAVFIA
LWCILVYSPICHWVWASDGFLFNLGPSGAIDFAGGTVVHISSGITALVAALYLGKRRGYPSTAMSPNNLVMTLIGAGLLW
VGWFGFNAGSAIASDLVTARALTVTQVAAASGAFAWMIIEMFHHGKATSLGVASGILAGLVAITPAAGVVQPIGAFALGA
LAAAICYSAILLKSKLGYDDSLDAFGVHGVGGIVGALALVFFIRPSWLADAAAKTANNSWTMWDQLGVQATAVGVTIAYA
AVISFVLLFIVEKTVGLRISEDDEMSGLDHSMHGEQGYGLINLN

Specific function: Involved in the uptake of ammonia [H]

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family [H]

Homologues:

Organism=Escherichia coli, GI1786656, Length=422, Percent_Identity=40.2843601895735, Blast_Score=273, Evalue=2e-74,
Organism=Caenorhabditis elegans, GI133931100, Length=433, Percent_Identity=27.9445727482679, Blast_Score=142, Evalue=5e-34,
Organism=Caenorhabditis elegans, GI32564042, Length=420, Percent_Identity=27.8571428571429, Blast_Score=136, Evalue=3e-32,
Organism=Caenorhabditis elegans, GI17550180, Length=435, Percent_Identity=28.2758620689655, Blast_Score=128, Evalue=5e-30,
Organism=Caenorhabditis elegans, GI17550182, Length=444, Percent_Identity=27.4774774774775, Blast_Score=120, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6324187, Length=422, Percent_Identity=34.8341232227488, Blast_Score=215, Evalue=1e-56,
Organism=Saccharomyces cerevisiae, GI6325396, Length=424, Percent_Identity=31.3679245283019, Blast_Score=214, Evalue=2e-56,
Organism=Saccharomyces cerevisiae, GI6321559, Length=426, Percent_Identity=30.2816901408451, Blast_Score=199, Evalue=8e-52,
Organism=Drosophila melanogaster, GI161078314, Length=410, Percent_Identity=31.7073170731707, Blast_Score=161, Evalue=9e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001905
- InterPro:   IPR018047 [H]

Pfam domain/function: PF00909 Ammonium_transp [H]

EC number: NA

Molecular weight: Translated: 46545; Mature: 46545

Theoretical pI: Translated: 6.43; Mature: 6.43

Prosite motif: PS01219 AMMONIUM_TRANSP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKHKHLTALLMLGALLLTGGNAWAADEVVTDSGTTSWMLTSTALVLLMIPGLAMFYGGLV
CCCHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
RTKNVLGTMMHSFGAMVIIGVLWPMVGYGLSFGPGILGGLAGWDDKFFFLQGIDDSIMST
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCCEEEEECCCHHHHHH
HIPEYVFAMFQGKFAIITPALIAGAFAERVNFKGYAVFIALWCILVYSPICHWVWASDGF
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCE
LFNLGPSGAIDFAGGTVVHISSGITALVAALYLGKRRGYPSTAMSPNNLVMTLIGAGLLW
EEECCCCCCEECCCCEEEEECCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHH
VGWFGFNAGSAIASDLVTARALTVTQVAAASGAFAWMIIEMFHHGKATSLGVASGILAGL
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHHHH
VAITPAAGVVQPIGAFALGALAAAICYSAILLKSKLGYDDSLDAFGVHGVGGIVGALALV
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHH
FFIRPSWLADAAAKTANNSWTMWDQLGVQATAVGVTIAYAAVISFVLLFIVEKTVGLRIS
HHHCCHHHHHHHHHCCCCCCEEHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEC
EDDEMSGLDHSMHGEQGYGLINLN
CCCCCCCCCCCCCCCCCCEEEEEC
>Mature Secondary Structure
MKHKHLTALLMLGALLLTGGNAWAADEVVTDSGTTSWMLTSTALVLLMIPGLAMFYGGLV
CCCHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
RTKNVLGTMMHSFGAMVIIGVLWPMVGYGLSFGPGILGGLAGWDDKFFFLQGIDDSIMST
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCCEEEEECCCHHHHHH
HIPEYVFAMFQGKFAIITPALIAGAFAERVNFKGYAVFIALWCILVYSPICHWVWASDGF
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCE
LFNLGPSGAIDFAGGTVVHISSGITALVAALYLGKRRGYPSTAMSPNNLVMTLIGAGLLW
EEECCCCCCEECCCCEEEEECCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHH
VGWFGFNAGSAIASDLVTARALTVTQVAAASGAFAWMIIEMFHHGKATSLGVASGILAGL
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHHHH
VAITPAAGVVQPIGAFALGALAAAICYSAILLKSKLGYDDSLDAFGVHGVGGIVGALALV
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHH
FFIRPSWLADAAAKTANNSWTMWDQLGVQATAVGVTIAYAAVISFVLLFIVEKTVGLRIS
HHHCCHHHHHHHHHCCCCCCEEHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEC
EDDEMSGLDHSMHGEQGYGLINLN
CCCCCCCCCCCCCCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]