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The map label for this gene is hmeA [H]

Identifier: 78189898

GI number: 78189898

Start: 2476408

End: 2477169

Strand: Reverse

Name: hmeA [H]

Synonym: Cag_1945

Alternate gene names: 78189898

Gene position: 2477169-2476408 (Counterclockwise)

Preceding gene: 78189899

Following gene: 78189897

Centisome position: 96.31

GC content: 47.64

Gene sequence:

>762_bases
ATGAACGAACAACGCAGAGAATTTATCCGATTGGCTGGCATGGGATTTCTTGCCAGTGTTGGTGCCACTTGTGGGCTTAT
GTCGAGCGCTCCATTAGAAGCCACCGATCTAACAGTGTTTGCTGATAGCGGCAATGGCGCTGTTCGCTGGGGTATGCTTG
TAGATACGCGCAAATGCCGCGAAGGGTGCAACAAGTGTATTGAAAGCTGCCATACGGTGCATAATGTGCCTGATTTTGCT
TCAGCAAAAGAGCGTGTGCAGTGGATTGAAAAGCGTCCAGTTGCTTCACTTTTTTCAGAGAGAAAAGCACTTCCAGCTCA
AAGTGCAACCTTACCTGCATTGTGTAACCATTGTGCGAAAGCGCCGTGTGTTACAGCCTGCCCCACCTCTGCAATATTCC
GCCGATATGACGGTATTGTTGGGCTTGATTTTCACCGTTGCATTGGTTGCCGTGCTTGCATGACGGCTTGCCCTTATAGC
GCTATTAGTTTTAATTGGAAAGCACCACGTCCAGCTATTAAGTCGTTAAGTGATGGTTATCCCACACGCGAGCAAGGTAT
GGTGGAAAAATGCAATTTCTGCTCGGAGCGCCTTGCTAAAGGCTTAATGCCTGCATGTGCGGAAAGTTGCCCTGAACATG
CGCTTGTATTTGGTAATCTTAATGATCCTGCTTCTGAAATTCGCACGTTGCTTGCCTCAAGCCGCACGTTACAGCGTAAA
GCGGAGCAAGGCACAAAACCTTCAGTTTTCTATATAATTTAA

Upstream 100 bases:

>100_bases
GATGCCATAGTACCAATAGTTACTTCTGTATTATGTGCCACGATTATACCCATGCAAAACCAAATTGCTGGAATTGTCAC
GTAGCGCCTTTTAAATAGTG

Downstream 100 bases:

>100_bases
GAGCCACCATGATTGAAAAAGCATTGAAAGGAAGCCCTACGTATTGGCTTTGGATTCTTCTGCTCCTTGCCTTGATGGGG
TTTGGCTACTCATCGTATGC

Product: polysulfide reductase, subunit B

Products: oxidized cytochrome C552; NH3 [C]

Alternate protein names: Hme subunit A [H]

Number of amino acids: Translated: 253; Mature: 253

Protein sequence:

>253_residues
MNEQRREFIRLAGMGFLASVGATCGLMSSAPLEATDLTVFADSGNGAVRWGMLVDTRKCREGCNKCIESCHTVHNVPDFA
SAKERVQWIEKRPVASLFSERKALPAQSATLPALCNHCAKAPCVTACPTSAIFRRYDGIVGLDFHRCIGCRACMTACPYS
AISFNWKAPRPAIKSLSDGYPTREQGMVEKCNFCSERLAKGLMPACAESCPEHALVFGNLNDPASEIRTLLASSRTLQRK
AEQGTKPSVFYII

Sequences:

>Translated_253_residues
MNEQRREFIRLAGMGFLASVGATCGLMSSAPLEATDLTVFADSGNGAVRWGMLVDTRKCREGCNKCIESCHTVHNVPDFA
SAKERVQWIEKRPVASLFSERKALPAQSATLPALCNHCAKAPCVTACPTSAIFRRYDGIVGLDFHRCIGCRACMTACPYS
AISFNWKAPRPAIKSLSDGYPTREQGMVEKCNFCSERLAKGLMPACAESCPEHALVFGNLNDPASEIRTLLASSRTLQRK
AEQGTKPSVFYII
>Mature_253_residues
MNEQRREFIRLAGMGFLASVGATCGLMSSAPLEATDLTVFADSGNGAVRWGMLVDTRKCREGCNKCIESCHTVHNVPDFA
SAKERVQWIEKRPVASLFSERKALPAQSATLPALCNHCAKAPCVTACPTSAIFRRYDGIVGLDFHRCIGCRACMTACPYS
AISFNWKAPRPAIKSLSDGYPTREQGMVEKCNFCSERLAKGLMPACAESCPEHALVFGNLNDPASEIRTLLASSRTLQRK
AEQGTKPSVFYII

Specific function: Has menaquinol-oxidizing activity. HmeA, hmeB and hmeE subunits may together catalyze electron transfer from menaquinol to cytochrome c [H]

COG id: COG0437

COG function: function code C; Fe-S-cluster-containing hydrogenase components 1

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein; Extracellular side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 4Fe-4S ferredoxin-type domain [H]

Homologues:

Organism=Escherichia coli, GI2367345, Length=254, Percent_Identity=35.4330708661417, Blast_Score=129, Evalue=3e-31,
Organism=Escherichia coli, GI226510944, Length=252, Percent_Identity=32.1428571428571, Blast_Score=111, Evalue=4e-26,
Organism=Escherichia coli, GI1787872, Length=208, Percent_Identity=30.7692307692308, Blast_Score=91, Evalue=9e-20,
Organism=Escherichia coli, GI1787122, Length=208, Percent_Identity=30.2884615384615, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1789370, Length=246, Percent_Identity=26.8292682926829, Blast_Score=84, Evalue=7e-18,
Organism=Escherichia coli, GI1787740, Length=134, Percent_Identity=35.0746268656716, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1787478, Length=101, Percent_Identity=41.5841584158416, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI87082114, Length=174, Percent_Identity=32.7586206896552, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1789067, Length=176, Percent_Identity=30.6818181818182, Blast_Score=71, Evalue=7e-14,
Organism=Escherichia coli, GI87082180, Length=187, Percent_Identity=27.2727272727273, Blast_Score=69, Evalue=2e-13,
Organism=Escherichia coli, GI87082179, Length=127, Percent_Identity=37.7952755905512, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1787749, Length=217, Percent_Identity=28.110599078341, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1790326, Length=127, Percent_Identity=32.2834645669291, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1789079, Length=181, Percent_Identity=29.2817679558011, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1788811, Length=196, Percent_Identity=26.0204081632653, Blast_Score=62, Evalue=3e-11,
Organism=Escherichia coli, GI2367245, Length=164, Percent_Identity=28.6585365853659, Blast_Score=61, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR006311 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 27606; Mature: 27606

Theoretical pI: Translated: 8.40; Mature: 8.40

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

6.7 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
9.5 %Cys+Met (Translated Protein)
6.7 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
9.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNEQRREFIRLAGMGFLASVGATCGLMSSAPLEATDLTVFADSGNGAVRWGMLVDTRKCR
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCEEEEHHHHHHHHHH
EGCNKCIESCHTVHNVPDFASAKERVQWIEKRPVASLFSERKALPAQSATLPALCNHCAK
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCCCHHHHHHHHHC
APCVTACPTSAIFRRYDGIVGLDFHRCIGCRACMTACPYSAISFNWKAPRPAIKSLSDGY
CCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCHHEECCCCCCCHHHHHHHCCC
PTREQGMVEKCNFCSERLAKGLMPACAESCPEHALVFGNLNDPASEIRTLLASSRTLQRK
CCHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHHHH
AEQGTKPSVFYII
HHCCCCCCEEEEC
>Mature Secondary Structure
MNEQRREFIRLAGMGFLASVGATCGLMSSAPLEATDLTVFADSGNGAVRWGMLVDTRKCR
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCEEEEHHHHHHHHHH
EGCNKCIESCHTVHNVPDFASAKERVQWIEKRPVASLFSERKALPAQSATLPALCNHCAK
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCCCHHHHHHHHHC
APCVTACPTSAIFRRYDGIVGLDFHRCIGCRACMTACPYSAISFNWKAPRPAIKSLSDGY
CCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCHHEECCCCCCCHHHHHHHCCC
PTREQGMVEKCNFCSERLAKGLMPACAESCPEHALVFGNLNDPASEIRTLLASSRTLQRK
CCHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHHHH
AEQGTKPSVFYII
HHCCCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: reduced cytochrome C552; nitrite [C]

Specific reaction: reduced cytochrome C552 + nitrite = oxidized cytochrome C552 + NH3 [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9389475; 11952791 [H]