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The map label for this gene is tuf

Identifier: 78189809

GI number: 78189809

Start: 2360547

End: 2361728

Strand: Reverse

Name: tuf

Synonym: Cag_1853

Alternate gene names: 78189809

Gene position: 2361728-2360547 (Counterclockwise)

Preceding gene: 78189810

Following gene: 78189808

Centisome position: 91.82

GC content: 44.59

Gene sequence:

>1182_bases
ATGGCAAAAGAGTCATACAAGAGGGATAAACCTCACGTTAATATAGGTACCATCGGCCACGTTGACCATGGCAAAACCAC
CCTTACTGCTGCAATTACATTAGTGCTTTCAAAGCAAGGTCTTGCTCAAGAGCGTGATTTCGGTAGCATTGATAAAGCTC
CTGAAGAAAGAGAGCGTGGTATTACCATTTCAACGGCACACGTTGAATATCAGACCGACAAGCGCCACTATGCTCACATT
GACTGTCCTGGTCACGCTGACTACATCAAGAACATGATCACTGGTGCTGCTCAGATGGATGGCGCAATTCTTGTTGTTGC
TGCAACTGACGGTCCTATGCCTCAAACTCGTGAGCACATTCTTCTTGCTCGTCAGGTAAACGTGCCTTCGCTTGTTGTTT
TCTTAAATAAAGTTGACATTGCAGATCCTGAACTTATTGAGTTGGTTGAGCTTGAGTTAAGAGAGCTTCTTTCTCAGTAT
GACTTCCCTGGTGATGATATTCCCATTATCAAAGGTTCTGCTTTAAAAGCTATGGAAGGTGATGCTGAAGGTGAAAAAGC
AATCCTTGAGCTTATGGATGCTGTTGATGCCTTTATTCCTGATCCTGTTCGCGATGTTGATAAGCCATTCTTAATGCCAG
TTGAAGATGTATTCTCAATTTCTGGTCGTGGCACGGTAGGTACGGGTAGAATTGAAAGAGGTCGTATTAAGCTCAACGAA
GAAGTTGAAATCGTTGGTCTTCGTCCAACCCGTAAGTCAGTTGTTACTGGTATCGAAATGTTCCAAAAACTCCTTGATGA
AGGTCAAGCTGGTGATAACGCTGGTCTCTTGCTTCGCGGTGTTGATAAAACGGAACTTGAGCGTGGTATGGTTATTGCTA
AACCAGGCACCATTAAGCCACACACCAAGTTTAAAGCTGAAGTATATATCTTGAAAAAAGAAGAAGGTGGGCGTCATACC
CCATTCTTCAATGGCTATCGCCCACAGTTCTATTTCCGTACCACCGACGTTACTGGTTCGGTAACGCTGCCTGAAGGTGT
TGAAATGGTTATGCCTGGCGATAACCTCGCAATTGAGGTTGAGCTTCTTGCTCCAATCGCTATGGACGAAGGTCTTCGCT
TTGCTATCCGCGAAGGTGGTCGTACTGTAGGAGCTGGTTCTGTAACCAAGATTAATGAGTAA

Upstream 100 bases:

>100_bases
AATCGCTTCAGGAAAAGCGCACAAGCAAAGATGCTGAGTAAAGAAGCGAGTTTCGATTTTATTATTACTAACACTAACTG
ATAACCGTACGGAGAAAGTT

Downstream 100 bases:

>100_bases
GAATTGATTGTATAGCGTCCCGGGGCGGGGCTTACTCCCGCCCCTTTTTTATTGATAACATGAAAATGGGTTGACAAGTG
GCTGTACAGCAAAAGATTAG

Product: elongation factor Tu

Products: GDP; phosphate

Alternate protein names: EF-Tu

Number of amino acids: Translated: 393; Mature: 392

Protein sequence:

>393_residues
MAKESYKRDKPHVNIGTIGHVDHGKTTLTAAITLVLSKQGLAQERDFGSIDKAPEERERGITISTAHVEYQTDKRHYAHI
DCPGHADYIKNMITGAAQMDGAILVVAATDGPMPQTREHILLARQVNVPSLVVFLNKVDIADPELIELVELELRELLSQY
DFPGDDIPIIKGSALKAMEGDAEGEKAILELMDAVDAFIPDPVRDVDKPFLMPVEDVFSISGRGTVGTGRIERGRIKLNE
EVEIVGLRPTRKSVVTGIEMFQKLLDEGQAGDNAGLLLRGVDKTELERGMVIAKPGTIKPHTKFKAEVYILKKEEGGRHT
PFFNGYRPQFYFRTTDVTGSVTLPEGVEMVMPGDNLAIEVELLAPIAMDEGLRFAIREGGRTVGAGSVTKINE

Sequences:

>Translated_393_residues
MAKESYKRDKPHVNIGTIGHVDHGKTTLTAAITLVLSKQGLAQERDFGSIDKAPEERERGITISTAHVEYQTDKRHYAHI
DCPGHADYIKNMITGAAQMDGAILVVAATDGPMPQTREHILLARQVNVPSLVVFLNKVDIADPELIELVELELRELLSQY
DFPGDDIPIIKGSALKAMEGDAEGEKAILELMDAVDAFIPDPVRDVDKPFLMPVEDVFSISGRGTVGTGRIERGRIKLNE
EVEIVGLRPTRKSVVTGIEMFQKLLDEGQAGDNAGLLLRGVDKTELERGMVIAKPGTIKPHTKFKAEVYILKKEEGGRHT
PFFNGYRPQFYFRTTDVTGSVTLPEGVEMVMPGDNLAIEVELLAPIAMDEGLRFAIREGGRTVGAGSVTKINE
>Mature_392_residues
AKESYKRDKPHVNIGTIGHVDHGKTTLTAAITLVLSKQGLAQERDFGSIDKAPEERERGITISTAHVEYQTDKRHYAHID
CPGHADYIKNMITGAAQMDGAILVVAATDGPMPQTREHILLARQVNVPSLVVFLNKVDIADPELIELVELELRELLSQYD
FPGDDIPIIKGSALKAMEGDAEGEKAILELMDAVDAFIPDPVRDVDKPFLMPVEDVFSISGRGTVGTGRIERGRIKLNEE
VEIVGLRPTRKSVVTGIEMFQKLLDEGQAGDNAGLLLRGVDKTELERGMVIAKPGTIKPHTKFKAEVYILKKEEGGRHTP
FFNGYRPQFYFRTTDVTGSVTLPEGVEMVMPGDNLAIEVELLAPIAMDEGLRFAIREGGRTVGAGSVTKINE

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis

COG id: COG0050

COG function: function code J; GTPases - translation elongation factors

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily

Homologues:

Organism=Homo sapiens, GI34147630, Length=392, Percent_Identity=57.1428571428571, Blast_Score=464, Evalue=1e-131,
Organism=Homo sapiens, GI223555963, Length=436, Percent_Identity=31.651376146789, Blast_Score=178, Evalue=8e-45,
Organism=Homo sapiens, GI5729864, Length=436, Percent_Identity=31.651376146789, Blast_Score=178, Evalue=9e-45,
Organism=Homo sapiens, GI4503475, Length=450, Percent_Identity=29.7777777777778, Blast_Score=166, Evalue=3e-41,
Organism=Homo sapiens, GI4503471, Length=450, Percent_Identity=29.5555555555556, Blast_Score=165, Evalue=7e-41,
Organism=Homo sapiens, GI54607086, Length=344, Percent_Identity=31.1046511627907, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI194018522, Length=338, Percent_Identity=29.8816568047337, Blast_Score=119, Evalue=3e-27,
Organism=Homo sapiens, GI194018520, Length=338, Percent_Identity=29.8816568047337, Blast_Score=119, Evalue=4e-27,
Organism=Homo sapiens, GI194097354, Length=338, Percent_Identity=29.585798816568, Blast_Score=119, Evalue=4e-27,
Organism=Homo sapiens, GI46094014, Length=350, Percent_Identity=29.1428571428571, Blast_Score=117, Evalue=1e-26,
Organism=Homo sapiens, GI4503507, Length=269, Percent_Identity=28.6245353159851, Blast_Score=77, Evalue=4e-14,
Organism=Homo sapiens, GI157426893, Length=167, Percent_Identity=33.5329341317365, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI94966754, Length=134, Percent_Identity=36.5671641791045, Blast_Score=71, Evalue=2e-12,
Organism=Escherichia coli, GI1790412, Length=392, Percent_Identity=72.4489795918367, Blast_Score=587, Evalue=1e-169,
Organism=Escherichia coli, GI1789737, Length=392, Percent_Identity=72.4489795918367, Blast_Score=587, Evalue=1e-169,
Organism=Escherichia coli, GI2367247, Length=377, Percent_Identity=29.4429708222812, Blast_Score=134, Evalue=1e-32,
Organism=Escherichia coli, GI48994988, Length=241, Percent_Identity=34.4398340248963, Blast_Score=106, Evalue=3e-24,
Organism=Escherichia coli, GI1789108, Length=254, Percent_Identity=30.7086614173228, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1788922, Length=141, Percent_Identity=36.8794326241135, Blast_Score=69, Evalue=7e-13,
Organism=Escherichia coli, GI1789738, Length=146, Percent_Identity=32.1917808219178, Blast_Score=62, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI17556456, Length=398, Percent_Identity=51.2562814070352, Blast_Score=384, Evalue=1e-107,
Organism=Caenorhabditis elegans, GI25141371, Length=370, Percent_Identity=44.5945945945946, Blast_Score=329, Evalue=2e-90,
Organism=Caenorhabditis elegans, GI17552884, Length=456, Percent_Identity=32.0175438596491, Blast_Score=174, Evalue=7e-44,
Organism=Caenorhabditis elegans, GI17569207, Length=456, Percent_Identity=32.0175438596491, Blast_Score=174, Evalue=7e-44,
Organism=Caenorhabditis elegans, GI32566303, Length=433, Percent_Identity=31.6397228637413, Blast_Score=152, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI115532067, Length=438, Percent_Identity=29.6803652968037, Blast_Score=136, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI115532065, Length=438, Percent_Identity=29.6803652968037, Blast_Score=135, Evalue=3e-32,
Organism=Caenorhabditis elegans, GI32566629, Length=445, Percent_Identity=26.7415730337079, Blast_Score=127, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI17506081, Length=301, Percent_Identity=31.2292358803987, Blast_Score=119, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI32566301, Length=149, Percent_Identity=43.6241610738255, Blast_Score=97, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17509919, Length=277, Percent_Identity=29.2418772563177, Blast_Score=82, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI17557151, Length=231, Percent_Identity=27.2727272727273, Blast_Score=80, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6324761, Length=397, Percent_Identity=66.2468513853904, Blast_Score=525, Evalue=1e-150,
Organism=Saccharomyces cerevisiae, GI6325337, Length=449, Percent_Identity=30.2895322939866, Blast_Score=152, Evalue=6e-38,
Organism=Saccharomyces cerevisiae, GI6319594, Length=449, Percent_Identity=30.2895322939866, Blast_Score=152, Evalue=6e-38,
Organism=Saccharomyces cerevisiae, GI6322937, Length=460, Percent_Identity=23.9130434782609, Blast_Score=94, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6320377, Length=345, Percent_Identity=28.9855072463768, Blast_Score=90, Evalue=7e-19,
Organism=Saccharomyces cerevisiae, GI6320863, Length=278, Percent_Identity=29.136690647482, Blast_Score=80, Evalue=4e-16,
Organism=Saccharomyces cerevisiae, GI6323320, Length=238, Percent_Identity=29.8319327731092, Blast_Score=76, Evalue=8e-15,
Organism=Saccharomyces cerevisiae, GI6323098, Length=169, Percent_Identity=33.7278106508876, Blast_Score=73, Evalue=9e-14,
Organism=Drosophila melanogaster, GI281363316, Length=389, Percent_Identity=57.3264781491003, Blast_Score=472, Evalue=1e-133,
Organism=Drosophila melanogaster, GI17864358, Length=389, Percent_Identity=57.3264781491003, Blast_Score=472, Evalue=1e-133,
Organism=Drosophila melanogaster, GI19921738, Length=385, Percent_Identity=48.0519480519481, Blast_Score=390, Evalue=1e-109,
Organism=Drosophila melanogaster, GI45553807, Length=455, Percent_Identity=32.3076923076923, Blast_Score=183, Evalue=2e-46,
Organism=Drosophila melanogaster, GI45553816, Length=455, Percent_Identity=32.3076923076923, Blast_Score=183, Evalue=2e-46,
Organism=Drosophila melanogaster, GI24651721, Length=455, Percent_Identity=32.3076923076923, Blast_Score=183, Evalue=2e-46,
Organism=Drosophila melanogaster, GI17864154, Length=455, Percent_Identity=32.3076923076923, Blast_Score=183, Evalue=2e-46,
Organism=Drosophila melanogaster, GI24652838, Length=450, Percent_Identity=32, Blast_Score=181, Evalue=1e-45,
Organism=Drosophila melanogaster, GI17137572, Length=450, Percent_Identity=32, Blast_Score=181, Evalue=1e-45,
Organism=Drosophila melanogaster, GI19922690, Length=304, Percent_Identity=33.5526315789474, Blast_Score=145, Evalue=5e-35,
Organism=Drosophila melanogaster, GI45550900, Length=447, Percent_Identity=28.4116331096197, Blast_Score=134, Evalue=9e-32,
Organism=Drosophila melanogaster, GI17137380, Length=348, Percent_Identity=28.1609195402299, Blast_Score=115, Evalue=3e-26,
Organism=Drosophila melanogaster, GI24647046, Length=279, Percent_Identity=27.9569892473118, Blast_Score=79, Evalue=5e-15,
Organism=Drosophila melanogaster, GI24647048, Length=279, Percent_Identity=27.9569892473118, Blast_Score=79, Evalue=6e-15,
Organism=Drosophila melanogaster, GI28572034, Length=165, Percent_Identity=36.969696969697, Blast_Score=69, Evalue=7e-12,
Organism=Drosophila melanogaster, GI78706572, Length=239, Percent_Identity=26.3598326359833, Blast_Score=68, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24582462, Length=131, Percent_Identity=35.1145038167939, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: 20568 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): EFTU_CHLCH (Q3APH1)

Other databases:

- EMBL:   CP000108
- RefSeq:   YP_380147.1
- HSSP:   P0A6N1
- ProteinModelPortal:   Q3APH1
- SMR:   Q3APH1
- STRING:   Q3APH1
- GeneID:   3747234
- GenomeReviews:   CP000108_GR
- KEGG:   cch:Cag_1853
- eggNOG:   COG0050
- HOGENOM:   HBG307581
- OMA:   IAMEKEV
- ProtClustDB:   PRK00049
- BioCyc:   CCHL340177:CAG_1853-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00118_B
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR004541
- InterPro:   IPR004160
- InterPro:   IPR009000
- PANTHER:   PTHR23115:SF31
- PRINTS:   PR00315
- TIGRFAMs:   TIGR00485
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3; SSF50465 Elong_init_C; SSF50447 Translat_factor

EC number: 3.6.5.3

Molecular weight: Translated: 43103; Mature: 42972

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKESYKRDKPHVNIGTIGHVDHGKTTLTAAITLVLSKQGLAQERDFGSIDKAPEERERG
CCCCCCCCCCCCEEECEEECCCCCCHHHHHHHEEECCCCCCCCCCCCCCCCCCCHHHHCC
ITISTAHVEYQTDKRHYAHIDCPGHADYIKNMITGAAQMDGAILVVAATDGPMPQTREHI
CEEEEEEEEEECCCCEEEEEECCCHHHHHHHHHHCHHHCCCEEEEEEECCCCCCCCHHHE
LLARQVNVPSLVVFLNKVDIADPELIELVELELRELLSQYDFPGDDIPIIKGSALKAMEG
EEEEECCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCCCEECCC
DAEGEKAILELMDAVDAFIPDPVRDVDKPFLMPVEDVFSISGRGTVGTGRIERGRIKLNE
CCCHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCHHHHHCCCCCCCCCCCCEECCEEEECC
EVEIVGLRPTRKSVVTGIEMFQKLLDEGQAGDNAGLLLRGVDKTELERGMVIAKPGTIKP
CEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCHHHHHCCEEEECCCCCCC
HTKFKAEVYILKKEEGGRHTPFFNGYRPQFYFRTTDVTGSVTLPEGVEMVMPGDNLAIEV
CCCEEEEEEEEEECCCCCCCCCCCCCCCEEEEEEECCCCEEECCCCCEEEECCCCEEEEE
ELLAPIAMDEGLRFAIREGGRTVGAGSVTKINE
EEEECEECCCCCEEEECCCCCEECCCCEEECCC
>Mature Secondary Structure 
AKESYKRDKPHVNIGTIGHVDHGKTTLTAAITLVLSKQGLAQERDFGSIDKAPEERERG
CCCCCCCCCCCEEECEEECCCCCCHHHHHHHEEECCCCCCCCCCCCCCCCCCCHHHHCC
ITISTAHVEYQTDKRHYAHIDCPGHADYIKNMITGAAQMDGAILVVAATDGPMPQTREHI
CEEEEEEEEEECCCCEEEEEECCCHHHHHHHHHHCHHHCCCEEEEEEECCCCCCCCHHHE
LLARQVNVPSLVVFLNKVDIADPELIELVELELRELLSQYDFPGDDIPIIKGSALKAMEG
EEEEECCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCCCEECCC
DAEGEKAILELMDAVDAFIPDPVRDVDKPFLMPVEDVFSISGRGTVGTGRIERGRIKLNE
CCCHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCHHHHHCCCCCCCCCCCCEECCEEEECC
EVEIVGLRPTRKSVVTGIEMFQKLLDEGQAGDNAGLLLRGVDKTELERGMVIAKPGTIKP
CEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCHHHHHCCEEEECCCCCCC
HTKFKAEVYILKKEEGGRHTPFFNGYRPQFYFRTTDVTGSVTLPEGVEMVMPGDNLAIEV
CCCEEEEEEEEEECCCCCCCCCCCCCCCEEEEEEECCCCEEECCCCCEEEECCCCEEEEE
ELLAPIAMDEGLRFAIREGGRTVGAGSVTKINE
EEEECEECCCCCEEEECCCCCEECCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA