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The map label for this gene is purA

Identifier: 78189772

GI number: 78189772

Start: 2338086

End: 2339390

Strand: Reverse

Name: purA

Synonym: Cag_1816

Alternate gene names: 78189772

Gene position: 2339390-2338086 (Counterclockwise)

Preceding gene: 78189773

Following gene: 78189771

Centisome position: 90.95

GC content: 44.83

Gene sequence:

>1305_bases
GTGGAATCAAAAAACTTCAAGCTCCCTTCACCATTAGCAACTGTGATTGTTGGTACTCAGTTTGGTGATGAAGGCAAAGG
AAAGTTGGTGGACTATCTTTCCGCAAACTATGACATTGTTGTACGTTATCAAGGTGGCGCCAATGCGGGGCACACGATTT
GCTTTGATGGTAAAAGCGTTGTGTTGCACTTAGTTCCTTCAGGAATTTTTCATGAGGGATGTACTTGCGTTATTGGTAAC
GGCGTTGTTATTGATCCTGTTGCCTTGCTTGAGGAAATTAAAACGGTTGAAAATTTAGGTTACGATGTTCGTGGGCGACT
TTTTATTAGTCATAATGCTCACCTCATTATGCCATACCATAAGCTCCTTGATTCGCTGCATGAAAGTGCTCAAGGCGACC
AAAAAATTGGCACTACTGGGCGTGGTATAGGTCCAAGTTATGAAGATAAAGTTGCGCGTAAAGGTATTCGTGTCGTGGAT
TTACTCAATCCTGAACTCTTAAAAGAGAAGCTGCGCGAAAATCTTTCGGCAAAAAATAAGCTGCTCCGCAATATCTACGA
CCGTGAAGAAATTGATGTGGAGGCAATGGTGCAGGAGTATGAAGAGTTCGATAAAATTATTGACCCTTATGTTACCAACA
CGCAAGCTTATTTAACCCGTGAGTTGCAAGCAGGTAAAACGGTGCTGCTTGAAGGCGCACAAGGTTGCTTGCTTGATGTT
GATCACGGCACCTATCCCTATGTTACCTCATCAAACCCCACATCGGGTGGCGCATGTACAGGTTCGGGAATAGCTCCAAA
TTATATTGGCAAAGTGATCGGTATTACAAAAGCATACATGACGCGCGTTGGCAACGGTGCTTTTCCAACCGAGTTGCTTG
ATGAGACTGGTGAGTTGCTGGGCAAAATTGGGCATGAATTTGGCGCTACAACCGGTAGAAAACGGCGTTGTGGTTGGCTT
GATTTAGTTGCTTTGCGCTATTCGCTTGCCATTAACGGTGTAACGGAAATAGCGCTTACAAAGCTTGATGTGCTTGATAG
CTTTGAGGAAATTAAGGTTTGTACGGCTTACCTACTTGATGGCAAAGAGCTGCACGACTTCCCTACCGATCACCAAACGC
TTGCTCAAGTTACCCCTGTTTATACCACACTGAAAGGATGGATGGCTTCAAATGCTGCGGCTCGTACTTTTGAGGAAATG
CAGCTTGAAGCTCAGCGTTATGTTGATTTTCTTGAAGAGCAAGTGCAGTTGCCCGTAACCTTTATTTCCGTTGGACCTGG
GCGCGATGAAACGGTGTTCCGCTAA

Upstream 100 bases:

>100_bases
CAAATTTGTTGTAAATTATTCATCCTTTTCGGGCGCAGGTAAAGCTCTGCTCCTCTCGCATATTGATCACTCAATTATCT
CTCAATAAATCAGCATTATC

Downstream 100 bases:

>100_bases
ATTCCATTTCTCTCGTATGGCTTTAATGGGAGTACTCGAACGGTAACGGTATTAGGAGAAATTATTATATCTTCGCATTC
TTTTTTTATGGGCAGGTACA

Product: adenylosuccinate synthetase

Products: NA

Alternate protein names: AMPSase; AdSS; IMP--aspartate ligase

Number of amino acids: Translated: 434; Mature: 434

Protein sequence:

>434_residues
MESKNFKLPSPLATVIVGTQFGDEGKGKLVDYLSANYDIVVRYQGGANAGHTICFDGKSVVLHLVPSGIFHEGCTCVIGN
GVVIDPVALLEEIKTVENLGYDVRGRLFISHNAHLIMPYHKLLDSLHESAQGDQKIGTTGRGIGPSYEDKVARKGIRVVD
LLNPELLKEKLRENLSAKNKLLRNIYDREEIDVEAMVQEYEEFDKIIDPYVTNTQAYLTRELQAGKTVLLEGAQGCLLDV
DHGTYPYVTSSNPTSGGACTGSGIAPNYIGKVIGITKAYMTRVGNGAFPTELLDETGELLGKIGHEFGATTGRKRRCGWL
DLVALRYSLAINGVTEIALTKLDVLDSFEEIKVCTAYLLDGKELHDFPTDHQTLAQVTPVYTTLKGWMASNAAARTFEEM
QLEAQRYVDFLEEQVQLPVTFISVGPGRDETVFR

Sequences:

>Translated_434_residues
MESKNFKLPSPLATVIVGTQFGDEGKGKLVDYLSANYDIVVRYQGGANAGHTICFDGKSVVLHLVPSGIFHEGCTCVIGN
GVVIDPVALLEEIKTVENLGYDVRGRLFISHNAHLIMPYHKLLDSLHESAQGDQKIGTTGRGIGPSYEDKVARKGIRVVD
LLNPELLKEKLRENLSAKNKLLRNIYDREEIDVEAMVQEYEEFDKIIDPYVTNTQAYLTRELQAGKTVLLEGAQGCLLDV
DHGTYPYVTSSNPTSGGACTGSGIAPNYIGKVIGITKAYMTRVGNGAFPTELLDETGELLGKIGHEFGATTGRKRRCGWL
DLVALRYSLAINGVTEIALTKLDVLDSFEEIKVCTAYLLDGKELHDFPTDHQTLAQVTPVYTTLKGWMASNAAARTFEEM
QLEAQRYVDFLEEQVQLPVTFISVGPGRDETVFR
>Mature_434_residues
MESKNFKLPSPLATVIVGTQFGDEGKGKLVDYLSANYDIVVRYQGGANAGHTICFDGKSVVLHLVPSGIFHEGCTCVIGN
GVVIDPVALLEEIKTVENLGYDVRGRLFISHNAHLIMPYHKLLDSLHESAQGDQKIGTTGRGIGPSYEDKVARKGIRVVD
LLNPELLKEKLRENLSAKNKLLRNIYDREEIDVEAMVQEYEEFDKIIDPYVTNTQAYLTRELQAGKTVLLEGAQGCLLDV
DHGTYPYVTSSNPTSGGACTGSGIAPNYIGKVIGITKAYMTRVGNGAFPTELLDETGELLGKIGHEFGATTGRKRRCGWL
DLVALRYSLAINGVTEIALTKLDVLDSFEEIKVCTAYLLDGKELHDFPTDHQTLAQVTPVYTTLKGWMASNAAARTFEEM
QLEAQRYVDFLEEQVQLPVTFISVGPGRDETVFR

Specific function: Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP

COG id: COG0104

COG function: function code F; Adenylosuccinate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the adenylosuccinate synthetase family

Homologues:

Organism=Homo sapiens, GI22748717, Length=424, Percent_Identity=46.4622641509434, Blast_Score=371, Evalue=1e-103,
Organism=Homo sapiens, GI34577063, Length=426, Percent_Identity=46.4788732394366, Blast_Score=369, Evalue=1e-102,
Organism=Homo sapiens, GI40316944, Length=394, Percent_Identity=45.4314720812183, Blast_Score=332, Evalue=3e-91,
Organism=Escherichia coli, GI1790620, Length=420, Percent_Identity=50, Blast_Score=417, Evalue=1e-118,
Organism=Caenorhabditis elegans, GI25146553, Length=430, Percent_Identity=44.8837209302326, Blast_Score=354, Evalue=4e-98,
Organism=Caenorhabditis elegans, GI25146555, Length=430, Percent_Identity=44.8837209302326, Blast_Score=354, Evalue=6e-98,
Organism=Saccharomyces cerevisiae, GI6324109, Length=433, Percent_Identity=44.3418013856813, Blast_Score=370, Evalue=1e-103,
Organism=Drosophila melanogaster, GI21356033, Length=421, Percent_Identity=46.0807600950119, Blast_Score=374, Evalue=1e-104,

Paralogues:

None

Copy number: 940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): PURA_CHLCH (Q3APK8)

Other databases:

- EMBL:   CP000108
- RefSeq:   YP_380110.1
- ProteinModelPortal:   Q3APK8
- SMR:   Q3APK8
- STRING:   Q3APK8
- GeneID:   3746931
- GenomeReviews:   CP000108_GR
- KEGG:   cch:Cag_1816
- eggNOG:   COG0104
- HOGENOM:   HBG658237
- OMA:   DYVVRYQ
- ProtClustDB:   PRK01117
- BioCyc:   CCHL340177:CAG_1816-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00011
- InterPro:   IPR018220
- InterPro:   IPR001114
- PANTHER:   PTHR11846
- SMART:   SM00788
- TIGRFAMs:   TIGR00184

Pfam domain/function: PF00709 Adenylsucc_synt

EC number: =6.3.4.4

Molecular weight: Translated: 47724; Mature: 47724

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: PS01266 ADENYLOSUCCIN_SYN_1; PS00513 ADENYLOSUCCIN_SYN_2

Important sites: ACT_SITE 23-23 ACT_SITE 51-51 BINDING 139-139 BINDING 153-153 BINDING 234-234 BINDING 249-249 BINDING 313-313 BINDING 315-315

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MESKNFKLPSPLATVIVGTQFGDEGKGKLVDYLSANYDIVVRYQGGANAGHTICFDGKSV
CCCCCCCCCCCCEEEEEECCCCCCCCCHHHHHHCCCCEEEEEECCCCCCCCEEEECCCEE
VLHLVPSGIFHEGCTCVIGNGVVIDPVALLEEIKTVENLGYDVRGRLFISHNAHLIMPYH
EEEECCCCHHHCCCEEEECCCEEECHHHHHHHHHHHHHCCCCCEEEEEEECCCEEEEHHH
KLLDSLHESAQGDQKIGTTGRGIGPSYEDKVARKGIRVVDLLNPELLKEKLRENLSAKNK
HHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHH
LLRNIYDREEIDVEAMVQEYEEFDKIIDPYVTNTQAYLTRELQAGKTVLLEGAQGCLLDV
HHHHHHCHHHCCHHHHHHHHHHHHHHHCCHHCCCHHHHHHHHHCCCEEEEECCCCCEEEC
DHGTYPYVTSSNPTSGGACTGSGIAPNYIGKVIGITKAYMTRVGNGAFPTELLDETGELL
CCCCCCEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
GKIGHEFGATTGRKRRCGWLDLVALRYSLAINGVTEIALTKLDVLDSFEEIKVCTAYLLD
HHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHC
GKELHDFPTDHQTLAQVTPVYTTLKGWMASNAAARTFEEMQLEAQRYVDFLEEQVQLPVT
CCHHCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEE
FISVGPGRDETVFR
EEEECCCCCCCCCC
>Mature Secondary Structure
MESKNFKLPSPLATVIVGTQFGDEGKGKLVDYLSANYDIVVRYQGGANAGHTICFDGKSV
CCCCCCCCCCCCEEEEEECCCCCCCCCHHHHHHCCCCEEEEEECCCCCCCCEEEECCCEE
VLHLVPSGIFHEGCTCVIGNGVVIDPVALLEEIKTVENLGYDVRGRLFISHNAHLIMPYH
EEEECCCCHHHCCCEEEECCCEEECHHHHHHHHHHHHHCCCCCEEEEEEECCCEEEEHHH
KLLDSLHESAQGDQKIGTTGRGIGPSYEDKVARKGIRVVDLLNPELLKEKLRENLSAKNK
HHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHH
LLRNIYDREEIDVEAMVQEYEEFDKIIDPYVTNTQAYLTRELQAGKTVLLEGAQGCLLDV
HHHHHHCHHHCCHHHHHHHHHHHHHHHCCHHCCCHHHHHHHHHCCCEEEEECCCCCEEEC
DHGTYPYVTSSNPTSGGACTGSGIAPNYIGKVIGITKAYMTRVGNGAFPTELLDETGELL
CCCCCCEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
GKIGHEFGATTGRKRRCGWLDLVALRYSLAINGVTEIALTKLDVLDSFEEIKVCTAYLLD
HHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHC
GKELHDFPTDHQTLAQVTPVYTTLKGWMASNAAARTFEEMQLEAQRYVDFLEEQVQLPVT
CCHHCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEE
FISVGPGRDETVFR
EEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA