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The map label for this gene is tilS [H]

Identifier: 78189702

GI number: 78189702

Start: 2264365

End: 2265429

Strand: Reverse

Name: tilS [H]

Synonym: Cag_1746

Alternate gene names: 78189702

Gene position: 2265429-2264365 (Counterclockwise)

Preceding gene: 78189709

Following gene: 78189696

Centisome position: 88.08

GC content: 47.14

Gene sequence:

>1065_bases
ATGACGCATGTTAACGCCGTTATTGTGCAATACTTCTTTACAGGCTCACTCTTAACCATCAACCTTACCAAGCAGACAAA
CAACCTGATGACTCCGCTTGAAAAACAATTTCTTGAGCAACTTACCAAGCGCCGCTTAGTGCAGCGTGGCGATAAGGTAC
TGCTTGCGGTTTCAGGCGGTCCCGATTCTATGGCGCTCCTTTCGCTTTTTTGCGCCGTACAACCATTTTTGCAATGCTCG
TTAGCGCTTGCCCACTGCAATTTTCAACTTCGTGATAGCGAAAGTGATAGTGATGAAGCTTTTGTGCAAGAGCAGGCAAC
CGCACGCAATCTTCCATGCTTTGTGGAGCGCTTTGAGACGCGCAATGTGGCAGCAACGTGGAAAAAATCGCTTGAAGAGA
GTGCTCGACTCTTGCGCTATGCCTTTTTTGAGCGCCTTATGCAAGTGCATGGTTTCCACAAAGTAGCTACAGGGCACCAT
GTAAACGACAACGCCGAAACCATTCTCTTTAACCTTGTACGAGGCACCTCATTGCTTGGTTTGCGAGGCATTCGTGCCCA
ACATGGCACTATTATTCGCCCAATGCTTTTATTGCATAAACCTGCAATTACCACCTACTTACATGAGCAGCAAATTCCAT
ATCGGGTTGATAGCAGTAACGAAGGTACCGAGTACGATCGCAATTTTATTCGCCATCGGCTTATTCCGCTTATTGAAGAG
CGTTTTCCCCGCAAATTACTGCCCTCACTGCAAAGGCTGTCGGAGCAAGCGGGCGAGCTGGAGGAGTTCTTAGAACTTTA
TTTCGAGCAGCTTACCCAACGTGAACCCTTGTTGCAACTACACAATAACCAGCTTAACGTTAAGGCTTTGCGACAGTTAA
CCACCTTTGAACAAAAAGAAATTATCAAACGAGCGCTTTGGGAACTTGGTGCTCCAGTTGATGGGCAAACGTTGCAACGA
ATGGTAGAATTACTACAAACGCAGTCGGGGCGAATGGTGCAACTTGGCAGAAAGTGGCAGGTTGAGTGGAAAGGCAACTC
CCTTTATTTTTCCCACCAACCATAG

Upstream 100 bases:

>100_bases
GTGAGCATGAATGGAATTGGGTCTTTTTTTACTTCTCGCTAAAAGGCGCTTATAATAATATTTTAAATCGTACCATTGCT
ATGGCTCCGTGCTTTTTAGT

Downstream 100 bases:

>100_bases
TTACGGCGCAGGTTGCTCTTTTGCCGTTTGAAAAATTTGGAGTGGCAAAGCGGTATGAATAATCACCGAATTTAAATTTT
GCTGAATAGCAATGTTATCC

Product: MesJ protein

Products: NA

Alternate protein names: tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase [H]

Number of amino acids: Translated: 354; Mature: 353

Protein sequence:

>354_residues
MTHVNAVIVQYFFTGSLLTINLTKQTNNLMTPLEKQFLEQLTKRRLVQRGDKVLLAVSGGPDSMALLSLFCAVQPFLQCS
LALAHCNFQLRDSESDSDEAFVQEQATARNLPCFVERFETRNVAATWKKSLEESARLLRYAFFERLMQVHGFHKVATGHH
VNDNAETILFNLVRGTSLLGLRGIRAQHGTIIRPMLLLHKPAITTYLHEQQIPYRVDSSNEGTEYDRNFIRHRLIPLIEE
RFPRKLLPSLQRLSEQAGELEEFLELYFEQLTQREPLLQLHNNQLNVKALRQLTTFEQKEIIKRALWELGAPVDGQTLQR
MVELLQTQSGRMVQLGRKWQVEWKGNSLYFSHQP

Sequences:

>Translated_354_residues
MTHVNAVIVQYFFTGSLLTINLTKQTNNLMTPLEKQFLEQLTKRRLVQRGDKVLLAVSGGPDSMALLSLFCAVQPFLQCS
LALAHCNFQLRDSESDSDEAFVQEQATARNLPCFVERFETRNVAATWKKSLEESARLLRYAFFERLMQVHGFHKVATGHH
VNDNAETILFNLVRGTSLLGLRGIRAQHGTIIRPMLLLHKPAITTYLHEQQIPYRVDSSNEGTEYDRNFIRHRLIPLIEE
RFPRKLLPSLQRLSEQAGELEEFLELYFEQLTQREPLLQLHNNQLNVKALRQLTTFEQKEIIKRALWELGAPVDGQTLQR
MVELLQTQSGRMVQLGRKWQVEWKGNSLYFSHQP
>Mature_353_residues
THVNAVIVQYFFTGSLLTINLTKQTNNLMTPLEKQFLEQLTKRRLVQRGDKVLLAVSGGPDSMALLSLFCAVQPFLQCSL
ALAHCNFQLRDSESDSDEAFVQEQATARNLPCFVERFETRNVAATWKKSLEESARLLRYAFFERLMQVHGFHKVATGHHV
NDNAETILFNLVRGTSLLGLRGIRAQHGTIIRPMLLLHKPAITTYLHEQQIPYRVDSSNEGTEYDRNFIRHRLIPLIEER
FPRKLLPSLQRLSEQAGELEEFLELYFEQLTQREPLLQLHNNQLNVKALRQLTTFEQKEIIKRALWELGAPVDGQTLQRM
VELLQTQSGRMVQLGRKWQVEWKGNSLYFSHQP

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA(Ile)-lysidine synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786386, Length=210, Percent_Identity=29.0476190476191, Blast_Score=69, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012094
- InterPro:   IPR012795
- InterPro:   IPR014729
- InterPro:   IPR011063 [H]

Pfam domain/function: PF01171 ATP_bind_3 [H]

EC number: NA

Molecular weight: Translated: 40964; Mature: 40833

Theoretical pI: Translated: 8.88; Mature: 8.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTHVNAVIVQYFFTGSLLTINLTKQTNNLMTPLEKQFLEQLTKRRLVQRGDKVLLAVSGG
CCCHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEECC
PDSMALLSLFCAVQPFLQCSLALAHCNFQLRDSESDSDEAFVQEQATARNLPCFVERFET
CCHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCHHHHHHHHHHHCCCCHHHHHHHC
RNVAATWKKSLEESARLLRYAFFERLMQVHGFHKVATGHHVNDNAETILFNLVRGTSLLG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCHHHHHHHHHHCCHHHH
LRGIRAQHGTIIRPMLLLHKPAITTYLHEQQIPYRVDSSNEGTEYDRNFIRHRLIPLIEE
HCCCCCCCCCHHHHHHHHHCCHHHHHHHHCCCCEEECCCCCCCHHHHHHHHHHHHHHHHH
RFPRKLLPSLQRLSEQAGELEEFLELYFEQLTQREPLLQLHNNQLNVKALRQLTTFEQKE
HCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCHHHHHHHHHHHHHH
IIKRALWELGAPVDGQTLQRMVELLQTQSGRMVQLGRKWQVEWKGNSLYFSHQP
HHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCEEEECCEEEEEECCCEEEECCCC
>Mature Secondary Structure 
THVNAVIVQYFFTGSLLTINLTKQTNNLMTPLEKQFLEQLTKRRLVQRGDKVLLAVSGG
CCHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEECC
PDSMALLSLFCAVQPFLQCSLALAHCNFQLRDSESDSDEAFVQEQATARNLPCFVERFET
CCHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCHHHHHHHHHHHCCCCHHHHHHHC
RNVAATWKKSLEESARLLRYAFFERLMQVHGFHKVATGHHVNDNAETILFNLVRGTSLLG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCHHHHHHHHHHCCHHHH
LRGIRAQHGTIIRPMLLLHKPAITTYLHEQQIPYRVDSSNEGTEYDRNFIRHRLIPLIEE
HCCCCCCCCCHHHHHHHHHCCHHHHHHHHCCCCEEECCCCCCCHHHHHHHHHHHHHHHHH
RFPRKLLPSLQRLSEQAGELEEFLELYFEQLTQREPLLQLHNNQLNVKALRQLTTFEQKE
HCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCHHHHHHHHHHHHHH
IIKRALWELGAPVDGQTLQRMVELLQTQSGRMVQLGRKWQVEWKGNSLYFSHQP
HHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCEEEECCEEEEEECCCEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10976061; 12093901 [H]