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The map label for this gene is sppA [H]

Identifier: 78189660

GI number: 78189660

Start: 2214894

End: 2216699

Strand: Reverse

Name: sppA [H]

Synonym: Cag_1704

Alternate gene names: 78189660

Gene position: 2216699-2214894 (Counterclockwise)

Preceding gene: 78189661

Following gene: 78189659

Centisome position: 86.18

GC content: 49.34

Gene sequence:

>1806_bases
ATGAATAATAGTTCCATTCCACAAAAGCGTCGCGGCTGTTTTCGCCCGGGCTGCTTATGGTTTTTAGTTGTGCCGCTCTT
TATTGTGGTTGCACTTTTTTGGGCGTTTCGCTCTTCGCACGATATGCCCGATCGTTTTGTGTTGGTTGTTCCTCTTAGTG
GCAAATTAGCCGAAGTCAATAACGAGCGCTCCTCACTGCCCTTTATGCCATCACAAGGCGATTTATCGCTGCAAGAGGTG
CTCTTTGTGCTGCACGAAGCTGCCAAGGATGAGCAAGTAAGTGAAGTGCTGCTGCAGCTTGATGGCGTTGAAGCTGCACC
CGCTAAAATTGCCGAAGTACGCGCGGCTGTTGCTGACGTGCGCCGCAAAGGCAAAAAGGTGAGCGCATTTTTATACCGTG
CAGAGGATAGCGATTACTTGCTTGCTACTGCGGCTGATACCATTATTATGCAACGCGGTGCTTCGCTTTTGCTGGATGGC
TTAAAAGCGGAGTCGCTTTTTTATACGGGAACATTAAACAAGCTCGGCATTACCGTACAAGCCGCTCAATGGAAAGAGTA
CAAAAGCGGCATTGAGCCTTTTACCCGCACAAGTGCCAGCAAAGAATACCGTGAGCAAATCAACATGCTGCTTGATGATG
TTTACAACAACTACCTTTCAGCCGTAAGCGAACGGCGTAAAATAAGCCGATCGGCATTTGAGGCTATTATTAATAACGAG
GCGTTGCTTTCGGCAGAACGTGCTAAAGCGCTTGGTCTTGTTGACCGCATTGCAACTTTTTGGGATGTAGAGCGCTCTAT
GACCAAACAGCTTACGGGCGAAGAGCTAAGTAGCGAGAATAATGCGCTGGTTCATGCTGCCGATTACCGCAATGCAATGG
ATTACCCGCAACACTCCAGCACAAGCGATGCCATTGCCGTTATTACCATGTCGGGTCCCATTATGCGCTCGGTAGATAAC
CTTGATGACGGCATTGATGTCGCCACTATGCAACATTCGCTTGAAGCTGCCCTTGAAAACAAGAGCGTCAAAGCCATTGT
GCTCCGCATTGATAGCCCGGGTGGCGAAGCTATTGCCTCAGCCGATATTTTGCAAATGATTAACGCTGCTGCTACCAAAA
AAACGCTTGTCGTCTCAATGTCGGGCGTTGCTGCATCAGGCGGTTACATGGTAGCGCTTGGCGGCAAAACCATTGTAGCA
CATCCGCTCACTATTACGGGTTCCATTGGCGTTTATGCGCTCAAACCAACCATTCAAGGATTGGCTGAAAAGGTTGGCTT
GCAACGCGAAGTTATTACAAGAGGACGTTTTGCTGATGCCACTTCACCCTTTACTCCGCTTGAAGGAGAAGCCTACAACA
AATTTGTAGCCTCAGCAGGCGACGTCTATAACGACTTTATCAGCAAAGTTGCAACATCACGCCGCATGAAGGTAACAGCC
GTTGACTCTGTTGCAGGCGGACGGGTATGGACGGGCAGCCGTGCCAAGCAAGTTGGTTTGGTTGACCGCATGGGTGGGCT
TTTTGATGCCCTTGCTTTAGCCAAAGAGCGTGCAGGCATTAGCAAAGATAAAGAGCCAACCATTCTCCTCTATCCCCTTC
AGCAAGGATGGCTACAATCGCTGCTGGGTGGCGCTACCCTCAATTCAGTAACCAAAGCAATTGCAACCGCGCTTCTCGGT
AACGTTTTACCAATAAACGTGGAGCAACAGCCACTTTCCGCCATGCAACCATTTTACGATATGCTGATTCGTTCAGGCAA
ACCGCACATGGTAGCACTTATGCCCGCTGAAGTGGTGGTGAAGTAA

Upstream 100 bases:

>100_bases
GTTTTTCATCCACGCTGCCCGCAAGCAAAGGCAGAGTGCCGTCATCATATTCCACAATTACAAGAACACTCATCGGGGCA
TCAAGTAGCCTGTCTTCTTT

Downstream 100 bases:

>100_bases
GGGATGTTTTTTTGGAGAGATGTGTTTTATAGGAATGTAGAAATAAGGAGAGATTATGACTTTTGCTGAATTTAAAGTTC
AGTTAGAAAACGCTGCAACC

Product: peptidase S49, protease IV

Products: NA

Alternate protein names: Endopeptidase IV; Protease IV homolog; Signal peptide peptidase [H]

Number of amino acids: Translated: 601; Mature: 601

Protein sequence:

>601_residues
MNNSSIPQKRRGCFRPGCLWFLVVPLFIVVALFWAFRSSHDMPDRFVLVVPLSGKLAEVNNERSSLPFMPSQGDLSLQEV
LFVLHEAAKDEQVSEVLLQLDGVEAAPAKIAEVRAAVADVRRKGKKVSAFLYRAEDSDYLLATAADTIIMQRGASLLLDG
LKAESLFYTGTLNKLGITVQAAQWKEYKSGIEPFTRTSASKEYREQINMLLDDVYNNYLSAVSERRKISRSAFEAIINNE
ALLSAERAKALGLVDRIATFWDVERSMTKQLTGEELSSENNALVHAADYRNAMDYPQHSSTSDAIAVITMSGPIMRSVDN
LDDGIDVATMQHSLEAALENKSVKAIVLRIDSPGGEAIASADILQMINAAATKKTLVVSMSGVAASGGYMVALGGKTIVA
HPLTITGSIGVYALKPTIQGLAEKVGLQREVITRGRFADATSPFTPLEGEAYNKFVASAGDVYNDFISKVATSRRMKVTA
VDSVAGGRVWTGSRAKQVGLVDRMGGLFDALALAKERAGISKDKEPTILLYPLQQGWLQSLLGGATLNSVTKAIATALLG
NVLPINVEQQPLSAMQPFYDMLIRSGKPHMVALMPAEVVVK

Sequences:

>Translated_601_residues
MNNSSIPQKRRGCFRPGCLWFLVVPLFIVVALFWAFRSSHDMPDRFVLVVPLSGKLAEVNNERSSLPFMPSQGDLSLQEV
LFVLHEAAKDEQVSEVLLQLDGVEAAPAKIAEVRAAVADVRRKGKKVSAFLYRAEDSDYLLATAADTIIMQRGASLLLDG
LKAESLFYTGTLNKLGITVQAAQWKEYKSGIEPFTRTSASKEYREQINMLLDDVYNNYLSAVSERRKISRSAFEAIINNE
ALLSAERAKALGLVDRIATFWDVERSMTKQLTGEELSSENNALVHAADYRNAMDYPQHSSTSDAIAVITMSGPIMRSVDN
LDDGIDVATMQHSLEAALENKSVKAIVLRIDSPGGEAIASADILQMINAAATKKTLVVSMSGVAASGGYMVALGGKTIVA
HPLTITGSIGVYALKPTIQGLAEKVGLQREVITRGRFADATSPFTPLEGEAYNKFVASAGDVYNDFISKVATSRRMKVTA
VDSVAGGRVWTGSRAKQVGLVDRMGGLFDALALAKERAGISKDKEPTILLYPLQQGWLQSLLGGATLNSVTKAIATALLG
NVLPINVEQQPLSAMQPFYDMLIRSGKPHMVALMPAEVVVK
>Mature_601_residues
MNNSSIPQKRRGCFRPGCLWFLVVPLFIVVALFWAFRSSHDMPDRFVLVVPLSGKLAEVNNERSSLPFMPSQGDLSLQEV
LFVLHEAAKDEQVSEVLLQLDGVEAAPAKIAEVRAAVADVRRKGKKVSAFLYRAEDSDYLLATAADTIIMQRGASLLLDG
LKAESLFYTGTLNKLGITVQAAQWKEYKSGIEPFTRTSASKEYREQINMLLDDVYNNYLSAVSERRKISRSAFEAIINNE
ALLSAERAKALGLVDRIATFWDVERSMTKQLTGEELSSENNALVHAADYRNAMDYPQHSSTSDAIAVITMSGPIMRSVDN
LDDGIDVATMQHSLEAALENKSVKAIVLRIDSPGGEAIASADILQMINAAATKKTLVVSMSGVAASGGYMVALGGKTIVA
HPLTITGSIGVYALKPTIQGLAEKVGLQREVITRGRFADATSPFTPLEGEAYNKFVASAGDVYNDFISKVATSRRMKVTA
VDSVAGGRVWTGSRAKQVGLVDRMGGLFDALALAKERAGISKDKEPTILLYPLQQGWLQSLLGGATLNSVTKAIATALLG
NVLPINVEQQPLSAMQPFYDMLIRSGKPHMVALMPAEVVVK

Specific function: Digestion of the cleaved signal peptides. This activity is necessary to maintain proper secretion of mature proteins across the membrane [H]

COG id: COG0616

COG function: function code OU; Periplasmic serine proteases (ClpP class)

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S49 family [H]

Homologues:

Organism=Escherichia coli, GI1788064, Length=458, Percent_Identity=30.7860262008734, Blast_Score=176, Evalue=4e-45,
Organism=Escherichia coli, GI1787527, Length=189, Percent_Identity=29.6296296296296, Blast_Score=72, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004634
- InterPro:   IPR004635
- InterPro:   IPR002142 [H]

Pfam domain/function: PF01343 Peptidase_S49 [H]

EC number: 3.4.21.-

Molecular weight: Translated: 65090; Mature: 65090

Theoretical pI: Translated: 7.68; Mature: 7.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNSSIPQKRRGCFRPGCLWFLVVPLFIVVALFWAFRSSHDMPDRFVLVVPLSGKLAEVN
CCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCEECCC
NERSSLPFMPSQGDLSLQEVLFVLHEAAKDEQVSEVLLQLDGVEAAPAKIAEVRAAVADV
CCCCCCCCCCCCCCCCHHHHHHHHHHHHCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
RRKGKKVSAFLYRAEDSDYLLATAADTIIMQRGASLLLDGLKAESLFYTGTLNKLGITVQ
HHCCCCEEEEEEECCCCCEEEEHHHHHHHHHCCHHHHHHCCCCCCEEEEECCHHHCEEEE
AAQWKEYKSGIEPFTRTSASKEYREQINMLLDDVYNNYLSAVSERRKISRSAFEAIINNE
HHHHHHHHHCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
ALLSAERAKALGLVDRIATFWDVERSMTKQLTGEELSSENNALVHAADYRNAMDYPQHSS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHCCCCCEEEEEHHHCCCCCCCCCCC
TSDAIAVITMSGPIMRSVDNLDDGIDVATMQHSLEAALENKSVKAIVLRIDSPGGEAIAS
CCCEEEEEEECCHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCHHHH
ADILQMINAAATKKTLVVSMSGVAASGGYMVALGGKTIVAHPLTITGSIGVYALKPTIQG
HHHHHHHHHHHCCCEEEEEECCCCCCCCEEEEECCCEEEEECEEEECCCCEEEECHHHHH
LAEKVGLQREVITRGRFADATSPFTPLEGEAYNKFVASAGDVYNDFISKVATSRRMKVTA
HHHHHCCHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHCCCEEEEE
VDSVAGGRVWTGSRAKQVGLVDRMGGLFDALALAKERAGISKDKEPTILLYPLQQGWLQS
ECCCCCCCEECCCCHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECHHHHHHH
LLGGATLNSVTKAIATALLGNVLPINVEQQPLSAMQPFYDMLIRSGKPHMVALMPAEVVV
HHCCHHHHHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHHHCCCCCEEEEECHHHHC
K
C
>Mature Secondary Structure
MNNSSIPQKRRGCFRPGCLWFLVVPLFIVVALFWAFRSSHDMPDRFVLVVPLSGKLAEVN
CCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCEECCC
NERSSLPFMPSQGDLSLQEVLFVLHEAAKDEQVSEVLLQLDGVEAAPAKIAEVRAAVADV
CCCCCCCCCCCCCCCCHHHHHHHHHHHHCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
RRKGKKVSAFLYRAEDSDYLLATAADTIIMQRGASLLLDGLKAESLFYTGTLNKLGITVQ
HHCCCCEEEEEEECCCCCEEEEHHHHHHHHHCCHHHHHHCCCCCCEEEEECCHHHCEEEE
AAQWKEYKSGIEPFTRTSASKEYREQINMLLDDVYNNYLSAVSERRKISRSAFEAIINNE
HHHHHHHHHCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
ALLSAERAKALGLVDRIATFWDVERSMTKQLTGEELSSENNALVHAADYRNAMDYPQHSS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHCCCCCEEEEEHHHCCCCCCCCCCC
TSDAIAVITMSGPIMRSVDNLDDGIDVATMQHSLEAALENKSVKAIVLRIDSPGGEAIAS
CCCEEEEEEECCHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCHHHH
ADILQMINAAATKKTLVVSMSGVAASGGYMVALGGKTIVAHPLTITGSIGVYALKPTIQG
HHHHHHHHHHHCCCEEEEEECCCCCCCCEEEEECCCEEEEECEEEECCCCEEEECHHHHH
LAEKVGLQREVITRGRFADATSPFTPLEGEAYNKFVASAGDVYNDFISKVATSRRMKVTA
HHHHHCCHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHCCCEEEEE
VDSVAGGRVWTGSRAKQVGLVDRMGGLFDALALAKERAGISKDKEPTILLYPLQQGWLQS
ECCCCCCCEECCCCHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECHHHHHHH
LLGGATLNSVTKAIATALLGNVLPINVEQQPLSAMQPFYDMLIRSGKPHMVALMPAEVVV
HHCCHHHHHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHHHCCCCCEEEEECHHHHC
K
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases) [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]