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The map label for this gene is yuxG [H]

Identifier: 78189599

GI number: 78189599

Start: 2140412

End: 2142529

Strand: Reverse

Name: yuxG [H]

Synonym: Cag_1642

Alternate gene names: 78189599

Gene position: 2142529-2140412 (Counterclockwise)

Preceding gene: 78189600

Following gene: 78189598

Centisome position: 83.3

GC content: 47.83

Gene sequence:

>2118_bases
ATGCAGAACCTTTGGAACGATGCAGAATTACAGGGCTTTGTGAGCAATGTGTGCCACGAGCCTGATGACCATCCTGAGCT
TGCAGCATTGGTGTATGCTTCGCGGTTGCTTGGGCGTGAACGTTCATTAGTAATGCACGGTGGTGGCAATACCTCCGTTA
AGTGTGGGCTTACCGATATGGTTGGCAACCATGCGGAAGTGTTGCTTATTAAAGCAAGTGGTATTGATTTAAGCAATGTT
ACCTGTCGCGATTATACCCCGCTCCGCTTAGGTCCACTGAGTAAACTTGTGGAGCTGTGCAGCAGTAACGATCCTATTCA
TGCTGAGCGTGTTGAACGTTTTTCAACGAAAGAGTTTAAGCATCTTTTAATGCTGAATATGTTCAGCCTTACTGACCATA
TGGCTGAAAAACGTTTAACTCCATCTATTGAAACGCTGCTCCATGCCTTTCTGCCTCATCGCTACATTTTACATACCCAC
TCATTTGCCTTGCTTACCATGAGCAATCAGCCAAACGGTGAAGCGCTCTGCCGCGAAACCTTAGGCGAGGCATTTGGCTC
GGTGCCTTACATTAAACCCGGTCTTGGTTTGGCGCGTGCCGCCGCTGGTGTGTATGAAGCACATCCCGCAATTGAGGGGC
TTGTGTTGCAAAAGCATGGCTTAGTAACTTTTGGTGAAACGGCTCAAGAGGCGTACAACCGCATGATTGACGCTGTTACA
AAACTTGAAGAGCGCATTGCTTTGGCTGGTCGTAAACCATTTACAACGGTTCCTTTGCCCGAAGAAATTGCAAAAGTGGA
AGATGTTGCTCCCATTATTCGTGGAGCTTGTGCTGAGGAAAAAGAGGTTGGTCGTCGCGATTATCAACATTTGATTCTTG
ATTTTCGTACCTCCGATGAAATTCTCACTTACGTCAATAGTGCCGATGTTGTGCGCATGAGTCAAAAAGGCTCCATGACG
CCCGATTTTATTATTCGTACAAAAAATAAGCCACTTGTGGTGCCTGCACCTGACGCAGCGGATCTTAACGGATTTAAAGC
TGCTGTTGATGAAGCGGTGCAGCGCTATCGTGATGCCTATATTGCCTACTTTAATGCGCAACAGCAAGCTTCAGGTATGG
AGGTTACCATGCTTGATCCTATGCCGCGTGTGGTGTTGGTGCCAGGGCTTGGGCTTTTTGGTTTAGGAAAAAGTGCCGCG
GCAGCCGCAGTGAATGCTGATATTGCTACCTGTACTGCCACTGCTATTCTTGATGCTGAATCGGTGGGTTCTTTTGAATC
CATTAGCGAGCGTGAAGCTTTTGATATTGAGTATTGGGATATGGAGCAGGCAAAAATCAATAAGGTGTATCACGGGACGT
TTGCGGGTAAAGTGGTGATGGTAACGGGAGGAGCAAGTGGCATTGGGCTTGCTACAGCCAAAGCATTTCGTCAGCGTGGT
GCTGAGTTAGTGGTGTTAGATCTTTCTCAAGAAGCGCTTGATAAAGCGGCTGAAGAGATTGGCGGTAATCCCTTAACGCT
TACCTGCAATGTTACCTCACGTGCTGATATTCGTGCGGCGTATGATGCGGTTTGCAAGCGTTATGGCGGTGTTGATGTAA
TTGTCTCCAACGTTGGTGCGGCTATTCAAGGGCGCATTGGCGATGTGTCGGATGAGTTGTTGCGCAAGAGTTTTGAAATT
AACTTTTTCTCCCACCACTACATTGCTCAAGAAGCGGTACGTGTGATGCGTTTGCAAGGCACGGGCGGTGTGTTGCTTTT
TAATGTTTCAAAGCAAGCGGTTAATCCAGGTCCCGATTTTGGACCTTACGGTTTACCAAAAGCTGCCACCATGTTTCTTG
TGCGCCAATATGCACTTGACCACGGTCGTGATGGCATTCGTGCAAACGGCATTAATGCCGACCGCATTCGCACCGGACTT
TTGACTGAAGAGATGATTAAATCGCGCTCGGCGGCGCGTGGTTTAAGCGAGCACGAATATATGGCTGGTAATTTGTTGCA
ACTTGAGGTATATGCTGAAGATGTGGCTGAAGCCTTTGTGCATTTAGCCCAAGAAATTCGCACCAACGCCGCAATCATTA
CCGTTGATGGTGGCAACATTGCTGCTACGTTGCGGTAG

Upstream 100 bases:

>100_bases
GCTGCATTTGCCGCTTTAAGTGGAGATGCTTTTAATTTTTGCTAATTCGTTGCTTAGCTCTTGAACGACGAAAGCAACCC
TTAGATGTTACTTACTTCTT

Downstream 100 bases:

>100_bases
AGTTGCTTGTCAACTCTTTGCATGAGCATCATTACAAAATAGAGCTATTTTAATAAACACTTCATTTTTAAAGAGGAGAG
CTATATGGCTGAATATGATA

Product: short chain dehydrogenase

Products: 3alpha12alpha-dihydroxy-oxobeta-cholanate; NADH [C]

Alternate protein names: ORF2 [H]

Number of amino acids: Translated: 705; Mature: 705

Protein sequence:

>705_residues
MQNLWNDAELQGFVSNVCHEPDDHPELAALVYASRLLGRERSLVMHGGGNTSVKCGLTDMVGNHAEVLLIKASGIDLSNV
TCRDYTPLRLGPLSKLVELCSSNDPIHAERVERFSTKEFKHLLMLNMFSLTDHMAEKRLTPSIETLLHAFLPHRYILHTH
SFALLTMSNQPNGEALCRETLGEAFGSVPYIKPGLGLARAAAGVYEAHPAIEGLVLQKHGLVTFGETAQEAYNRMIDAVT
KLEERIALAGRKPFTTVPLPEEIAKVEDVAPIIRGACAEEKEVGRRDYQHLILDFRTSDEILTYVNSADVVRMSQKGSMT
PDFIIRTKNKPLVVPAPDAADLNGFKAAVDEAVQRYRDAYIAYFNAQQQASGMEVTMLDPMPRVVLVPGLGLFGLGKSAA
AAAVNADIATCTATAILDAESVGSFESISEREAFDIEYWDMEQAKINKVYHGTFAGKVVMVTGGASGIGLATAKAFRQRG
AELVVLDLSQEALDKAAEEIGGNPLTLTCNVTSRADIRAAYDAVCKRYGGVDVIVSNVGAAIQGRIGDVSDELLRKSFEI
NFFSHHYIAQEAVRVMRLQGTGGVLLFNVSKQAVNPGPDFGPYGLPKAATMFLVRQYALDHGRDGIRANGINADRIRTGL
LTEEMIKSRSAARGLSEHEYMAGNLLQLEVYAEDVAEAFVHLAQEIRTNAAIITVDGGNIAATLR

Sequences:

>Translated_705_residues
MQNLWNDAELQGFVSNVCHEPDDHPELAALVYASRLLGRERSLVMHGGGNTSVKCGLTDMVGNHAEVLLIKASGIDLSNV
TCRDYTPLRLGPLSKLVELCSSNDPIHAERVERFSTKEFKHLLMLNMFSLTDHMAEKRLTPSIETLLHAFLPHRYILHTH
SFALLTMSNQPNGEALCRETLGEAFGSVPYIKPGLGLARAAAGVYEAHPAIEGLVLQKHGLVTFGETAQEAYNRMIDAVT
KLEERIALAGRKPFTTVPLPEEIAKVEDVAPIIRGACAEEKEVGRRDYQHLILDFRTSDEILTYVNSADVVRMSQKGSMT
PDFIIRTKNKPLVVPAPDAADLNGFKAAVDEAVQRYRDAYIAYFNAQQQASGMEVTMLDPMPRVVLVPGLGLFGLGKSAA
AAAVNADIATCTATAILDAESVGSFESISEREAFDIEYWDMEQAKINKVYHGTFAGKVVMVTGGASGIGLATAKAFRQRG
AELVVLDLSQEALDKAAEEIGGNPLTLTCNVTSRADIRAAYDAVCKRYGGVDVIVSNVGAAIQGRIGDVSDELLRKSFEI
NFFSHHYIAQEAVRVMRLQGTGGVLLFNVSKQAVNPGPDFGPYGLPKAATMFLVRQYALDHGRDGIRANGINADRIRTGL
LTEEMIKSRSAARGLSEHEYMAGNLLQLEVYAEDVAEAFVHLAQEIRTNAAIITVDGGNIAATLR
>Mature_705_residues
MQNLWNDAELQGFVSNVCHEPDDHPELAALVYASRLLGRERSLVMHGGGNTSVKCGLTDMVGNHAEVLLIKASGIDLSNV
TCRDYTPLRLGPLSKLVELCSSNDPIHAERVERFSTKEFKHLLMLNMFSLTDHMAEKRLTPSIETLLHAFLPHRYILHTH
SFALLTMSNQPNGEALCRETLGEAFGSVPYIKPGLGLARAAAGVYEAHPAIEGLVLQKHGLVTFGETAQEAYNRMIDAVT
KLEERIALAGRKPFTTVPLPEEIAKVEDVAPIIRGACAEEKEVGRRDYQHLILDFRTSDEILTYVNSADVVRMSQKGSMT
PDFIIRTKNKPLVVPAPDAADLNGFKAAVDEAVQRYRDAYIAYFNAQQQASGMEVTMLDPMPRVVLVPGLGLFGLGKSAA
AAAVNADIATCTATAILDAESVGSFESISEREAFDIEYWDMEQAKINKVYHGTFAGKVVMVTGGASGIGLATAKAFRQRG
AELVVLDLSQEALDKAAEEIGGNPLTLTCNVTSRADIRAAYDAVCKRYGGVDVIVSNVGAAIQGRIGDVSDELLRKSFEI
NFFSHHYIAQEAVRVMRLQGTGGVLLFNVSKQAVNPGPDFGPYGLPKAATMFLVRQYALDHGRDGIRANGINADRIRTGL
LTEEMIKSRSAARGLSEHEYMAGNLLQLEVYAEDVAEAFVHLAQEIRTNAAIITVDGGNIAATLR

Specific function: 7-Alpha-Dehydroxylation Of Cholic Acid, Yielding Deoxycholic Acid And Lithocholic Acid, Respectively. Highest Affinity With Taurochenodeoxycholic Acid. [C]

COG id: COG3347

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI32483357, Length=224, Percent_Identity=31.6964285714286, Blast_Score=93, Evalue=9e-19,
Organism=Homo sapiens, GI126723191, Length=190, Percent_Identity=31.0526315789474, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI40807363, Length=117, Percent_Identity=32.4786324786325, Blast_Score=70, Evalue=8e-12,
Organism=Homo sapiens, GI210032110, Length=163, Percent_Identity=25.7668711656442, Blast_Score=68, Evalue=3e-11,
Organism=Homo sapiens, GI5031737, Length=191, Percent_Identity=27.7486910994764, Blast_Score=67, Evalue=7e-11,
Organism=Escherichia coli, GI1787905, Length=250, Percent_Identity=30, Blast_Score=85, Evalue=2e-17,
Organism=Escherichia coli, GI87082100, Length=190, Percent_Identity=28.4210526315789, Blast_Score=77, Evalue=4e-15,
Organism=Escherichia coli, GI1789378, Length=198, Percent_Identity=29.7979797979798, Blast_Score=74, Evalue=4e-14,
Organism=Escherichia coli, GI1790717, Length=263, Percent_Identity=25.8555133079848, Blast_Score=73, Evalue=8e-14,
Organism=Caenorhabditis elegans, GI17561402, Length=198, Percent_Identity=31.3131313131313, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI193204405, Length=200, Percent_Identity=30, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17538182, Length=206, Percent_Identity=30.5825242718447, Blast_Score=76, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI17559104, Length=267, Percent_Identity=29.5880149812734, Blast_Score=74, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17544670, Length=270, Percent_Identity=26.6666666666667, Blast_Score=73, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI72000259, Length=280, Percent_Identity=31.0714285714286, Blast_Score=72, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17562906, Length=199, Percent_Identity=29.6482412060301, Blast_Score=71, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17560332, Length=271, Percent_Identity=26.5682656826568, Blast_Score=70, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17562904, Length=199, Percent_Identity=28.1407035175879, Blast_Score=70, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17560220, Length=203, Percent_Identity=30.0492610837438, Blast_Score=70, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17531453, Length=200, Percent_Identity=28.5, Blast_Score=69, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17563726, Length=259, Percent_Identity=27.027027027027, Blast_Score=68, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17508895, Length=132, Percent_Identity=29.5454545454545, Blast_Score=67, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17560676, Length=190, Percent_Identity=30.5263157894737, Blast_Score=66, Evalue=6e-11,
Organism=Caenorhabditis elegans, GI17562908, Length=200, Percent_Identity=29, Blast_Score=66, Evalue=8e-11,
Organism=Drosophila melanogaster, GI21355319, Length=191, Percent_Identity=29.8429319371728, Blast_Score=77, Evalue=6e-14,
Organism=Drosophila melanogaster, GI21357041, Length=196, Percent_Identity=28.5714285714286, Blast_Score=71, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24644339, Length=185, Percent_Identity=29.1891891891892, Blast_Score=69, Evalue=8e-12,
Organism=Drosophila melanogaster, GI24663911, Length=218, Percent_Identity=26.605504587156, Blast_Score=69, Evalue=9e-12,
Organism=Drosophila melanogaster, GI24640492, Length=169, Percent_Identity=27.810650887574, Blast_Score=68, Evalue=3e-11,
Organism=Drosophila melanogaster, GI28571526, Length=192, Percent_Identity=28.6458333333333, Blast_Score=67, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001303
- InterPro:   IPR013454
- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00106 adh_short; PF00596 Aldolase_II [H]

EC number: 1.1.1.159 [C]

Molecular weight: Translated: 76625; Mature: 76625

Theoretical pI: Translated: 5.81; Mature: 5.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQNLWNDAELQGFVSNVCHEPDDHPELAALVYASRLLGRERSLVMHGGGNTSVKCGLTDM
CCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCEEEECHHHH
VGNHAEVLLIKASGIDLSNVTCRDYTPLRLGPLSKLVELCSSNDPIHAERVERFSTKEFK
CCCCEEEEEEEECCCCCCCCEECCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
HLLMLNMFSLTDHMAEKRLTPSIETLLHAFLPHRYILHTHSFALLTMSNQPNGEALCRET
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCEEEEECCEEEEEECCCCCHHHHHHHH
LGEAFGSVPYIKPGLGLARAAAGVYEAHPAIEGLVLQKHGLVTFGETAQEAYNRMIDAVT
HHHHHCCCCCCCCCCCHHHHHHHHHHCCCHHHCEEEECCCCEEECHHHHHHHHHHHHHHH
KLEERIALAGRKPFTTVPLPEEIAKVEDVAPIIRGACAEEKEVGRRDYQHLILDFRTSDE
HHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHEEEECCCCH
ILTYVNSADVVRMSQKGSMTPDFIIRTKNKPLVVPAPDAADLNGFKAAVDEAVQRYRDAY
HHHHCCCCCEEEECCCCCCCCCEEEEECCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHE
IAYFNAQQQASGMEVTMLDPMPRVVLVPGLGLFGLGKSAAAAAVNADIATCTATAILDAE
EEEECCHHHCCCCEEEEECCCCCEEEECCCCCCCCCCCHHHHHHCCCHHHHHHHHEECHH
SVGSFESISEREAFDIEYWDMEQAKINKVYHGTFAGKVVMVTGGASGIGLATAKAFRQRG
HCCCHHHHCCCCCCCEEECCCCHHHHHHEEECEECCEEEEEECCCCCCHHHHHHHHHHCC
AELVVLDLSQEALDKAAEEIGGNPLTLTCNVTSRADIRAAYDAVCKRYGGVDVIVSNVGA
CEEEEEECCHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCEEEEECCCC
AIQGRIGDVSDELLRKSFEINFFSHHYIAQEAVRVMRLQGTGGVLLFNVSKQAVNPGPDF
HHCCCCCCHHHHHHHHHHCCEEHHHHHHHHHHHHHEEECCCCCEEEEECCHHCCCCCCCC
GPYGLPKAATMFLVRQYALDHGRDGIRANGINADRIRTGLLTEEMIKSRSAARGLSEHEY
CCCCCCHHHHHHHHHHHHHHCCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHH
MAGNLLQLEVYAEDVAEAFVHLAQEIRTNAAIITVDGGNIAATLR
HCCCEEEEEEHHHHHHHHHHHHHHHHCCCEEEEEEECCCEEEEEC
>Mature Secondary Structure
MQNLWNDAELQGFVSNVCHEPDDHPELAALVYASRLLGRERSLVMHGGGNTSVKCGLTDM
CCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCEEEECHHHH
VGNHAEVLLIKASGIDLSNVTCRDYTPLRLGPLSKLVELCSSNDPIHAERVERFSTKEFK
CCCCEEEEEEEECCCCCCCCEECCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
HLLMLNMFSLTDHMAEKRLTPSIETLLHAFLPHRYILHTHSFALLTMSNQPNGEALCRET
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCEEEEECCEEEEEECCCCCHHHHHHHH
LGEAFGSVPYIKPGLGLARAAAGVYEAHPAIEGLVLQKHGLVTFGETAQEAYNRMIDAVT
HHHHHCCCCCCCCCCCHHHHHHHHHHCCCHHHCEEEECCCCEEECHHHHHHHHHHHHHHH
KLEERIALAGRKPFTTVPLPEEIAKVEDVAPIIRGACAEEKEVGRRDYQHLILDFRTSDE
HHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHEEEECCCCH
ILTYVNSADVVRMSQKGSMTPDFIIRTKNKPLVVPAPDAADLNGFKAAVDEAVQRYRDAY
HHHHCCCCCEEEECCCCCCCCCEEEEECCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHE
IAYFNAQQQASGMEVTMLDPMPRVVLVPGLGLFGLGKSAAAAAVNADIATCTATAILDAE
EEEECCHHHCCCCEEEEECCCCCEEEECCCCCCCCCCCHHHHHHCCCHHHHHHHHEECHH
SVGSFESISEREAFDIEYWDMEQAKINKVYHGTFAGKVVMVTGGASGIGLATAKAFRQRG
HCCCHHHHCCCCCCCEEECCCCHHHHHHEEECEECCEEEEEECCCCCCHHHHHHHHHHCC
AELVVLDLSQEALDKAAEEIGGNPLTLTCNVTSRADIRAAYDAVCKRYGGVDVIVSNVGA
CEEEEEECCHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCEEEEECCCC
AIQGRIGDVSDELLRKSFEINFFSHHYIAQEAVRVMRLQGTGGVLLFNVSKQAVNPGPDF
HHCCCCCCHHHHHHHHHHCCEEHHHHHHHHHHHHHEEECCCCCEEEEECCHHCCCCCCCC
GPYGLPKAATMFLVRQYALDHGRDGIRANGINADRIRTGLLTEEMIKSRSAARGLSEHEY
CCCCCCHHHHHHHHHHHHHHCCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHH
MAGNLLQLEVYAEDVAEAFVHLAQEIRTNAAIITVDGGNIAATLR
HCCCEEEEEEHHHHHHHHHHHHHHHHCCCEEEEEEECCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: 2-Mercaptoethanol; Dithiothreitol; NAD+; NADH [C]

Metal ions: CaCl2; KCl; MnCl2; NaCl [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.8 {cholic} 0.085 {glycochenodeoxycholic} 0.24 {taurochenodeoxycholic} 1 {glycocholic} 0.06 {chenodeoxycholic} [C]

Substrates: @ALPH01.txt*3alpha7alpha12alpha-Trihydroxybeta-cholanate!; NAD [C]

Specific reaction: @ALPH01.txt*3alpha7alpha12alpha-Trihydroxybeta-cholanate! + NAD+ = 3alpha12alpha-dihydroxy-oxobeta-cholanate + NADH [C]

General reaction: Redox reaction [C]

Inhibitor: Ascorbicacid; BaCl2; CoCl2; CuCl2; EDTA; FeCl3; HgCl2; MgCl2; NH4Cl; Potassiumoxalate; Sodiumcitrate; Sodiumlaurylsulfate; Sodiumperchlorate; Sodiumperiodate; Sodiumpersulfate; TritonX-100; Tween; ZnCl2 [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9274030; 9384377; 8188684 [H]