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The map label for this gene is nusA

Identifier: 78189424

GI number: 78189424

Start: 1929886

End: 1931469

Strand: Reverse

Name: nusA

Synonym: Cag_1463

Alternate gene names: 78189424

Gene position: 1931469-1929886 (Counterclockwise)

Preceding gene: 78189425

Following gene: 78189423

Centisome position: 75.09

GC content: 40.66

Gene sequence:

>1584_bases
ATGGCTAAAAAGAAAATAAAGGACGAAGGGCAAGAACGGCGGTTACAAATTGCCAGCGCATTCGGTGAAATTGAGCAATC
CAAAATGTTTTTGGATAAGCGCACAGAAAGCGCCGCAGTAAAAATGGATATTGCCGACTTGCTTAAGGATATTATTCAAA
AACAATTACGGAAAGATTACGATCCTGAAGTTGAAGCCAATATTTTCATCAATCCTGAGCGCGGTGATTTTGAGGTTTAT
ATTTTACGTAAAATTGTTGAAGATGTGGATATTCCTACCATTGAAATTTCATTGGAAGATATTCGCAAAATAGATGATTC
GCTTGAAGTTGGCGATTATTATGAAGAAGGACCTATTAAGCTTGATGATTATTTAAGTCGCAAATCAATTCAAATTATTA
AACAATCAGTTCAAAAGAAAGTTCGCGATCTTGAGCGTTTAATTGTTTATGAAGAGTGTTTAGAAAAAGTTGGTGAAGTT
ATTGCGGGTGAAGTCTATCAAGTGCGTTCCAATGAGGTTATTTTTACCTACAACACCTCTAAAGATCATCGAGTAGAGTT
AGTGCTGCCAAAGTCGGAGATGATGAAAAAAGATAATCCTCGCCGTATGCCACGCATGAAGCTTTATGTAAAGCGCATTG
AGCGTGAAAAAAACAAAATCCGTATGGACGATGGCACGGTTGTGGAGCGCGAAAAGCCCGATGGAGGTATGAAAGTGATT
GTATCGCGTGTGGATGATCGCTTTTTATACAAACTCTTTGAACACGAAGTACCTGAAATTCTTGATGGGCTTATTATTAT
TAAAGCCGTGGCTCGTGTACCGGGTGAACGTGCCAAAGTTGCCGTAGAATCCACCAGTGCTCGCATTGATCCTGTTGGTG
CAACGGTAGGGTATCGTGGAAAGCGCATTCAAAGCATTGTAAAAGAACTCAATAATGAAAATATTGATGTGATTTATTAT
GCCGATGAGCCGCAAATTTATATTTCGCGTGCTATGCAGCCTGCGAAAATTGATCCTATGACGGTGCATGTGGATATTAA
AACTCGCAAAGCGCGTGTTATGCTTAAACCCGATCAAATTAAATATGCGATTGGTAAAAATGGCAACAATATCCATTTGG
CAGAAAAGCTGACTGGCTATGAAATAGATGTATATCGGGATGTAATGGATAAAACGACTGAAGATCCAACCGATATTGAT
ATTATTGAATTCCGTGAAGAGTTTGGCGATGATATGATTTACCAGCTTCTTGATGCTGGGCTTGATACCGCCAAAAAAGT
GTTGCGTGCTGGGCTTGAAGAAATTGAGTTAGCATTGCTTGGGCCAGCTAAAGTTGATGAGCCGCAGCTTTTTCATAAAG
CGCCAAAAGGCACTATACCGATGGTGCGTGAGCGCAAAATTACCGACGAGGAGCGCCGTTATTGGCATAAAATAGCCGAT
AATATTTATCGCACCGTTCGTGAGCAATTTAGTGATGATGATTTTGATGAGTTGTGGGATGAAGAGTTGTTGCCATCGGA
AGATGCACCGCTTGATTCTGCAACGGAGGTTGAGCCACAGCAAGATGGTGAAGGCGAAGACTAA

Upstream 100 bases:

>100_bases
AATATTAGGTTGCCATTAAGCAGAATTATAAAAGCGGTACCTGAAGCAGAATTATAGGAAAATTTTTAGTACACCATTTT
TTAATAACCTTGGGATTGAG

Downstream 100 bases:

>100_bases
ATAAGTTGTATTAACGATGAACATGAGCTGTTTATACTCGAACCATGGAGATTCCGTGATTGAGTGTAAGCGGCACCCGA
TACCGCAATAAGATTTTACC

Product: transcription elongation factor NusA

Products: NA

Alternate protein names: L factor; N utilization substance protein A [H]

Number of amino acids: Translated: 527; Mature: 526

Protein sequence:

>527_residues
MAKKKIKDEGQERRLQIASAFGEIEQSKMFLDKRTESAAVKMDIADLLKDIIQKQLRKDYDPEVEANIFINPERGDFEVY
ILRKIVEDVDIPTIEISLEDIRKIDDSLEVGDYYEEGPIKLDDYLSRKSIQIIKQSVQKKVRDLERLIVYEECLEKVGEV
IAGEVYQVRSNEVIFTYNTSKDHRVELVLPKSEMMKKDNPRRMPRMKLYVKRIEREKNKIRMDDGTVVEREKPDGGMKVI
VSRVDDRFLYKLFEHEVPEILDGLIIIKAVARVPGERAKVAVESTSARIDPVGATVGYRGKRIQSIVKELNNENIDVIYY
ADEPQIYISRAMQPAKIDPMTVHVDIKTRKARVMLKPDQIKYAIGKNGNNIHLAEKLTGYEIDVYRDVMDKTTEDPTDID
IIEFREEFGDDMIYQLLDAGLDTAKKVLRAGLEEIELALLGPAKVDEPQLFHKAPKGTIPMVRERKITDEERRYWHKIAD
NIYRTVREQFSDDDFDELWDEELLPSEDAPLDSATEVEPQQDGEGED

Sequences:

>Translated_527_residues
MAKKKIKDEGQERRLQIASAFGEIEQSKMFLDKRTESAAVKMDIADLLKDIIQKQLRKDYDPEVEANIFINPERGDFEVY
ILRKIVEDVDIPTIEISLEDIRKIDDSLEVGDYYEEGPIKLDDYLSRKSIQIIKQSVQKKVRDLERLIVYEECLEKVGEV
IAGEVYQVRSNEVIFTYNTSKDHRVELVLPKSEMMKKDNPRRMPRMKLYVKRIEREKNKIRMDDGTVVEREKPDGGMKVI
VSRVDDRFLYKLFEHEVPEILDGLIIIKAVARVPGERAKVAVESTSARIDPVGATVGYRGKRIQSIVKELNNENIDVIYY
ADEPQIYISRAMQPAKIDPMTVHVDIKTRKARVMLKPDQIKYAIGKNGNNIHLAEKLTGYEIDVYRDVMDKTTEDPTDID
IIEFREEFGDDMIYQLLDAGLDTAKKVLRAGLEEIELALLGPAKVDEPQLFHKAPKGTIPMVRERKITDEERRYWHKIAD
NIYRTVREQFSDDDFDELWDEELLPSEDAPLDSATEVEPQQDGEGED
>Mature_526_residues
AKKKIKDEGQERRLQIASAFGEIEQSKMFLDKRTESAAVKMDIADLLKDIIQKQLRKDYDPEVEANIFINPERGDFEVYI
LRKIVEDVDIPTIEISLEDIRKIDDSLEVGDYYEEGPIKLDDYLSRKSIQIIKQSVQKKVRDLERLIVYEECLEKVGEVI
AGEVYQVRSNEVIFTYNTSKDHRVELVLPKSEMMKKDNPRRMPRMKLYVKRIEREKNKIRMDDGTVVEREKPDGGMKVIV
SRVDDRFLYKLFEHEVPEILDGLIIIKAVARVPGERAKVAVESTSARIDPVGATVGYRGKRIQSIVKELNNENIDVIYYA
DEPQIYISRAMQPAKIDPMTVHVDIKTRKARVMLKPDQIKYAIGKNGNNIHLAEKLTGYEIDVYRDVMDKTTEDPTDIDI
IEFREEFGDDMIYQLLDAGLDTAKKVLRAGLEEIELALLGPAKVDEPQLFHKAPKGTIPMVRERKITDEERRYWHKIADN
IYRTVREQFSDDDFDELWDEELLPSEDAPLDSATEVEPQQDGEGED

Specific function: Participates in both the termination and antitermination of transcription. NusA binds directly to the core enzyme of the DNA-dependent RNA polymerase. NusA also interacts with lambda N protein, RNA, rho factor, and perhaps nusB. Binds RNA [H]

COG id: COG0195

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI1789560, Length=436, Percent_Identity=31.1926605504587, Blast_Score=179, Evalue=4e-46,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1612 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010995
- InterPro:   IPR004087
- InterPro:   IPR015946
- InterPro:   IPR009019
- InterPro:   IPR004088
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR022967
- InterPro:   IPR013735
- InterPro:   IPR010214
- InterPro:   IPR010213 [H]

Pfam domain/function: PF08529 NusA_N; PF00575 S1 [H]

EC number: NA

Molecular weight: Translated: 60998; Mature: 60867

Theoretical pI: Translated: 4.70; Mature: 4.70

Prosite motif: PS50084 KH_TYPE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKKKIKDEGQERRLQIASAFGEIEQSKMFLDKRTESAAVKMDIADLLKDIIQKQLRKDY
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHEEEECHHHHHHHHHHHHHHCCC
DPEVEANIFINPERGDFEVYILRKIVEDVDIPTIEISLEDIRKIDDSLEVGDYYEEGPIK
CCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCEEEEEHHHHHHHHCCCCCCCCCCCCCEE
LDDYLSRKSIQIIKQSVQKKVRDLERLIVYEECLEKVGEVIAGEVYQVRSNEVIFTYNTS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCEEEEEECC
KDHRVELVLPKSEMMKKDNPRRMPRMKLYVKRIEREKNKIRMDDGTVVEREKPDGGMKVI
CCCEEEEEECHHHHHCCCCCCCCHHHHHHHHHHHHHHHCEECCCCCEEECCCCCCCHHHH
VSRVDDRFLYKLFEHEVPEILDGLIIIKAVARVPGERAKVAVESTSARIDPVGATVGYRG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHEEEEECCCCCCCCCCCCCCCCH
KRIQSIVKELNNENIDVIYYADEPQIYISRAMQPAKIDPMTVHVDIKTRKARVMLKPDQI
HHHHHHHHHHCCCCEEEEEECCCCCHHHHHCCCCCCCCCEEEEEEEEECCEEEEECCCCE
KYAIGKNGNNIHLAEKLTGYEIDVYRDVMDKTTEDPTDIDIIEFREEFGDDMIYQLLDAG
EEEECCCCCEEEEEHHCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHCHHHHHHHHHCC
LDTAKKVLRAGLEEIELALLGPAKVDEPQLFHKAPKGTIPMVRERKITDEERRYWHKIAD
HHHHHHHHHHCHHHHHEEEECCCCCCCCHHHHCCCCCCCCCHHCCCCCHHHHHHHHHHHH
NIYRTVREQFSDDDFDELWDEELLPSEDAPLDSATEVEPQQDGEGED
HHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
AKKKIKDEGQERRLQIASAFGEIEQSKMFLDKRTESAAVKMDIADLLKDIIQKQLRKDY
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHEEEECHHHHHHHHHHHHHHCCC
DPEVEANIFINPERGDFEVYILRKIVEDVDIPTIEISLEDIRKIDDSLEVGDYYEEGPIK
CCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCEEEEEHHHHHHHHCCCCCCCCCCCCCEE
LDDYLSRKSIQIIKQSVQKKVRDLERLIVYEECLEKVGEVIAGEVYQVRSNEVIFTYNTS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCEEEEEECC
KDHRVELVLPKSEMMKKDNPRRMPRMKLYVKRIEREKNKIRMDDGTVVEREKPDGGMKVI
CCCEEEEEECHHHHHCCCCCCCCHHHHHHHHHHHHHHHCEECCCCCEEECCCCCCCHHHH
VSRVDDRFLYKLFEHEVPEILDGLIIIKAVARVPGERAKVAVESTSARIDPVGATVGYRG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHEEEEECCCCCCCCCCCCCCCCH
KRIQSIVKELNNENIDVIYYADEPQIYISRAMQPAKIDPMTVHVDIKTRKARVMLKPDQI
HHHHHHHHHHCCCCEEEEEECCCCCHHHHHCCCCCCCCCEEEEEEEEECCEEEEECCCCE
KYAIGKNGNNIHLAEKLTGYEIDVYRDVMDKTTEDPTDIDIIEFREEFGDDMIYQLLDAG
EEEECCCCCEEEEEHHCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHCHHHHHHHHHCC
LDTAKKVLRAGLEEIELALLGPAKVDEPQLFHKAPKGTIPMVRERKITDEERRYWHKIAD
HHHHHHHHHHCHHHHHEEEECCCCCCCCHHHHCCCCCCCCCHHCCCCCHHHHHHHHHHHH
NIYRTVREQFSDDDFDELWDEELLPSEDAPLDSATEVEPQQDGEGED
HHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8113180; 11677609 [H]