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The map label for this gene is pcrA [H]

Identifier: 78189378

GI number: 78189378

Start: 1884000

End: 1886231

Strand: Reverse

Name: pcrA [H]

Synonym: Cag_1415

Alternate gene names: 78189378

Gene position: 1886231-1884000 (Counterclockwise)

Preceding gene: 78189381

Following gene: 78189377

Centisome position: 73.33

GC content: 47.45

Gene sequence:

>2232_bases
TTGAGTAATTTTTTACACGATTTAAATGAGGTGCAGCGCTCTGCTGTTGAAGCTACCTCAGGTCCAGTAATGGTGCTGGC
AGGTGCAGGTTCAGGTAAAACAAGGGTTATTACCTACCGTATTGCCCATCTTATTAATAACGAAGGCATAGCGCCGCGCA
ATATTTTAGCGTTAACCTTTACCAATAAAGCGGCTGGTGAAATGCGGGAGCGTGTTGATACGCTCTTGCACCACGGGGCA
TCGCGTGGGTTGTGGATTGGCACCTTTCACTCCATTTTTGCCCGCTTGCTCCGCAATTCTATTGACCGAATTGGGTACGA
TCGCAACTTCTCTATTTTTGATGCCGACGATAGTCGTAGCTTAATTCGCCAATCTATGGCAGAGCTTGATATTTCGGCGG
ATGCGGTGCCGCTCAACACCTTGCAAAGCATTATTAGCCGTGCAAAAAATAGCTTTGTTATGCCGGCTGAGTTCCAGCGC
AACGCAAACGATTACAATCAGCAAAAAGCAGCGCAAGTTTATTCGCTCTACTGCAAAAAGCTGAAAGAGAACAATGCGCT
TGATTTTGATGATTTACTGATTAAGCCGCTTGAACTTTTTAATGCTCATCCTGATGTACTCCATGAATTGCAGGAGCTGT
TCCGTTACATTATGATTGATGAGTATCAGGATACCAACCGTGTGCAATACTTGGTAGCTAAAATGCTTGGAGCACGCCAT
CGCAATATTTTTGTGGTGGGCGACGATGCTCAATCCATTTACTCATGGCGTGGTGCCGATATTTCCAATATTTTGAATTT
TCAGGATGATTATCACGATGCTCAAACCTTTAAGTTGGTTGAAAATTATAGGAGCACGGGGAACATCTTAAAAGCTGCAA
ATAGCGTGATTTGCCGCAACCAACGGCAAATTAAAAAGGAGCTGGTGTCGCACCGTCATGCGGGTGAACCGCTCACCGTT
ATGGAGGCGTTCAACGAGCGCAATGAGGCTGAAAAGGTTGCCGACCGTATTCGCACCATGCGCATGAGTGGCACCAACGA
TTATCGTAGCTTTGCTATTTTTTACCGCACCAACGCCCAATCGCGCGTGCTTGAGGATATTATGCGCCAGCAACGCATTC
CCTACCGCCTGTTTGGCAGCGTTTCGTTTTACAAGCGCAAAGAAATTAAAGATGCGGTAGCCTACCTCCGCTTTATCGTA
AATGAGCGAGATAGCGAATCACTTTTACGTATTATTAATTTTCCACCCCGTAAAATTGGCGATGTCAGCATTGCTAAGTT
GCGCGATTTTGCGGAAGTGCGGCACATTAGCTTGTACGAAGCAATCCACCGAGCTGCTGAAGCTGGCTTTCCCGCACGTT
TGCTTAATGCGCTTGCTTCCTTCACCTCTGTGATTGAAGCGCTTCGCGAAATGGCAACACGCGGCACGGTGTATGATGTT
TTAAATGAACTCTTTACGCTTACCTCCATTCCGCTCCTCTTGCAAGCGGAAAATACCCCTGAGTCGCTTGCACGCCACGA
AAACTTGCAAGAGTTGCTCTCTATGGCACGCGATTTTGCCGACCACAATCCAGACGGTGGCTCGCTGGGTGATTTTCTTG
AAAATATCTCCCTTGCTTCCGATTACGATGAAACGCAGGAGTCGGATAATTATGTTTCATTAATGACGGTACACGCCTCC
AAAGGGTTGGAGTTCCCCGTTGTTTTTATTACAGGGCTTGAAGAGCGCCTGTTTCCGTTGCACACCTACGAACCTGAAGA
GCTTGAAGAGGAGCGCCGCTTGTTTTATGTGGCAATGACGCGCGCACAAGAGAAAATTTTTCTTTCGTATGCAAAAAGTC
GCTACCAATATGGGCAGTTGCACCAATCAATTGCCTCAACTTTTATTAGCGAAATTGATGCCTCCATTGTGCAATCGGAA
GGTGGGCGCTTACTTTCCGACCGCCGTGCACCACGCGAAGCTACCACTCCGCAAAATCATGCCGCAGCGCCAGCAATGCG
TCGTCCTACAACGTCAGGCATGGCACCAAGCAGTGCAAGTAGCCCTACGGCAGAGAGCAGTAGCCCGTCAATTAGCAACG
GCACCTTAGTGCATCACCCCTTGTTTGGGCAAGGTGTGGTGCTGGAAGTACAAGGCAAAGGCTCAAAACAAAAAGTGCGC
ATTGCTTTCCGCAATGCGGGCGAAAAAACATTAATGGTGCAGTATGCCAATTTGAAAATACAAACATCATGA

Upstream 100 bases:

>100_bases
TGGATTACCGAAAAGAGCATTACCGCAAAGCGCTCTCTGAGGTGAAGTATCTTTTAATGGGGAGCGGTTGAATAGTCAGT
AAACAGGGATTTATAAGGTT

Downstream 100 bases:

>100_bases
AAATTCTTTTTGGTGTTCAAGGAACCGGTAACGGTCATATTAGTCGCAGTCGCGAGCTTGTTCGTGCGCTTAAAGAAGCT
GGGCACGAGCTTGAAGTAAT

Product: DNA helicase II

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 743; Mature: 742

Protein sequence:

>743_residues
MSNFLHDLNEVQRSAVEATSGPVMVLAGAGSGKTRVITYRIAHLINNEGIAPRNILALTFTNKAAGEMRERVDTLLHHGA
SRGLWIGTFHSIFARLLRNSIDRIGYDRNFSIFDADDSRSLIRQSMAELDISADAVPLNTLQSIISRAKNSFVMPAEFQR
NANDYNQQKAAQVYSLYCKKLKENNALDFDDLLIKPLELFNAHPDVLHELQELFRYIMIDEYQDTNRVQYLVAKMLGARH
RNIFVVGDDAQSIYSWRGADISNILNFQDDYHDAQTFKLVENYRSTGNILKAANSVICRNQRQIKKELVSHRHAGEPLTV
MEAFNERNEAEKVADRIRTMRMSGTNDYRSFAIFYRTNAQSRVLEDIMRQQRIPYRLFGSVSFYKRKEIKDAVAYLRFIV
NERDSESLLRIINFPPRKIGDVSIAKLRDFAEVRHISLYEAIHRAAEAGFPARLLNALASFTSVIEALREMATRGTVYDV
LNELFTLTSIPLLLQAENTPESLARHENLQELLSMARDFADHNPDGGSLGDFLENISLASDYDETQESDNYVSLMTVHAS
KGLEFPVVFITGLEERLFPLHTYEPEELEEERRLFYVAMTRAQEKIFLSYAKSRYQYGQLHQSIASTFISEIDASIVQSE
GGRLLSDRRAPREATTPQNHAAAPAMRRPTTSGMAPSSASSPTAESSSPSISNGTLVHHPLFGQGVVLEVQGKGSKQKVR
IAFRNAGEKTLMVQYANLKIQTS

Sequences:

>Translated_743_residues
MSNFLHDLNEVQRSAVEATSGPVMVLAGAGSGKTRVITYRIAHLINNEGIAPRNILALTFTNKAAGEMRERVDTLLHHGA
SRGLWIGTFHSIFARLLRNSIDRIGYDRNFSIFDADDSRSLIRQSMAELDISADAVPLNTLQSIISRAKNSFVMPAEFQR
NANDYNQQKAAQVYSLYCKKLKENNALDFDDLLIKPLELFNAHPDVLHELQELFRYIMIDEYQDTNRVQYLVAKMLGARH
RNIFVVGDDAQSIYSWRGADISNILNFQDDYHDAQTFKLVENYRSTGNILKAANSVICRNQRQIKKELVSHRHAGEPLTV
MEAFNERNEAEKVADRIRTMRMSGTNDYRSFAIFYRTNAQSRVLEDIMRQQRIPYRLFGSVSFYKRKEIKDAVAYLRFIV
NERDSESLLRIINFPPRKIGDVSIAKLRDFAEVRHISLYEAIHRAAEAGFPARLLNALASFTSVIEALREMATRGTVYDV
LNELFTLTSIPLLLQAENTPESLARHENLQELLSMARDFADHNPDGGSLGDFLENISLASDYDETQESDNYVSLMTVHAS
KGLEFPVVFITGLEERLFPLHTYEPEELEEERRLFYVAMTRAQEKIFLSYAKSRYQYGQLHQSIASTFISEIDASIVQSE
GGRLLSDRRAPREATTPQNHAAAPAMRRPTTSGMAPSSASSPTAESSSPSISNGTLVHHPLFGQGVVLEVQGKGSKQKVR
IAFRNAGEKTLMVQYANLKIQTS
>Mature_742_residues
SNFLHDLNEVQRSAVEATSGPVMVLAGAGSGKTRVITYRIAHLINNEGIAPRNILALTFTNKAAGEMRERVDTLLHHGAS
RGLWIGTFHSIFARLLRNSIDRIGYDRNFSIFDADDSRSLIRQSMAELDISADAVPLNTLQSIISRAKNSFVMPAEFQRN
ANDYNQQKAAQVYSLYCKKLKENNALDFDDLLIKPLELFNAHPDVLHELQELFRYIMIDEYQDTNRVQYLVAKMLGARHR
NIFVVGDDAQSIYSWRGADISNILNFQDDYHDAQTFKLVENYRSTGNILKAANSVICRNQRQIKKELVSHRHAGEPLTVM
EAFNERNEAEKVADRIRTMRMSGTNDYRSFAIFYRTNAQSRVLEDIMRQQRIPYRLFGSVSFYKRKEIKDAVAYLRFIVN
ERDSESLLRIINFPPRKIGDVSIAKLRDFAEVRHISLYEAIHRAAEAGFPARLLNALASFTSVIEALREMATRGTVYDVL
NELFTLTSIPLLLQAENTPESLARHENLQELLSMARDFADHNPDGGSLGDFLENISLASDYDETQESDNYVSLMTVHASK
GLEFPVVFITGLEERLFPLHTYEPEELEEERRLFYVAMTRAQEKIFLSYAKSRYQYGQLHQSIASTFISEIDASIVQSEG
GRLLSDRRAPREATTPQNHAAAPAMRRPTTSGMAPSSASSPTAESSSPSISNGTLVHHPLFGQGVVLEVQGKGSKQKVRI
AFRNAGEKTLMVQYANLKIQTS

Specific function: Essential helicase. May act as a helicase in plasmid pT181 replication [H]

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI2367296, Length=747, Percent_Identity=35.475234270415, Blast_Score=450, Evalue=1e-127,
Organism=Escherichia coli, GI48994965, Length=670, Percent_Identity=37.910447761194, Blast_Score=408, Evalue=1e-115,
Organism=Saccharomyces cerevisiae, GI6322369, Length=736, Percent_Identity=27.0380434782609, Blast_Score=199, Evalue=1e-51,
Organism=Saccharomyces cerevisiae, GI6324477, Length=658, Percent_Identity=22.9483282674772, Blast_Score=117, Evalue=6e-27,

Paralogues:

None

Copy number: 3000 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005751
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 83999; Mature: 83868

Theoretical pI: Translated: 7.12; Mature: 7.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNFLHDLNEVQRSAVEATSGPVMVLAGAGSGKTRVITYRIAHLINNEGIAPRNILALTF
CCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCEEEEHHHHHHHHCCCCCCCCCEEEEEE
TNKAAGEMRERVDTLLHHGASRGLWIGTFHSIFARLLRNSIDRIGYDRNFSIFDADDSRS
CCHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCHHH
LIRQSMAELDISADAVPLNTLQSIISRAKNSFVMPAEFQRNANDYNQQKAAQVYSLYCKK
HHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCEECCHHHHCCCCHHHHHHHHHHHHHHHHH
LKENNALDFDDLLIKPLELFNAHPDVLHELQELFRYIMIDEYQDTNRVQYLVAKMLGARH
HHCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCC
RNIFVVGDDAQSIYSWRGADISNILNFQDDYHDAQTFKLVENYRSTGNILKAANSVICRN
CEEEEECCCHHHHHHCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
QRQIKKELVSHRHAGEPLTVMEAFNERNEAEKVADRIRTMRMSGTNDYRSFAIFYRTNAQ
HHHHHHHHHHHHCCCCCHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCH
SRVLEDIMRQQRIPYRLFGSVSFYKRKEIKDAVAYLRFIVNERDSESLLRIINFPPRKIG
HHHHHHHHHHHCCCEEECCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCC
DVSIAKLRDFAEVRHISLYEAIHRAAEAGFPARLLNALASFTSVIEALREMATRGTVYDV
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
LNELFTLTSIPLLLQAENTPESLARHENLQELLSMARDFADHNPDGGSLGDFLENISLAS
HHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCHHC
DYDETQESDNYVSLMTVHASKGLEFPVVFITGLEERLFPLHTYEPEELEEERRLFYVAMT
CCCHHHCCCCEEEEEEEECCCCCCCCEEEEECCHHHCCCCCCCCHHHHHHHHHEEHHHHH
RAQEKIFLSYAKSRYQYGQLHQSIASTFISEIDASIVQSEGGRLLSDRRAPREATTPQNH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCCCCCCCCCCC
AAAPAMRRPTTSGMAPSSASSPTAESSSPSISNGTLVHHPLFGQGVVLEVQGKGSKQKVR
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCEEEEEECCCCCCEEE
IAFRNAGEKTLMVQYANLKIQTS
EEEECCCCEEEEEEEECEEEECC
>Mature Secondary Structure 
SNFLHDLNEVQRSAVEATSGPVMVLAGAGSGKTRVITYRIAHLINNEGIAPRNILALTF
CHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCEEEEHHHHHHHHCCCCCCCCCEEEEEE
TNKAAGEMRERVDTLLHHGASRGLWIGTFHSIFARLLRNSIDRIGYDRNFSIFDADDSRS
CCHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCHHH
LIRQSMAELDISADAVPLNTLQSIISRAKNSFVMPAEFQRNANDYNQQKAAQVYSLYCKK
HHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCEECCHHHHCCCCHHHHHHHHHHHHHHHHH
LKENNALDFDDLLIKPLELFNAHPDVLHELQELFRYIMIDEYQDTNRVQYLVAKMLGARH
HHCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCC
RNIFVVGDDAQSIYSWRGADISNILNFQDDYHDAQTFKLVENYRSTGNILKAANSVICRN
CEEEEECCCHHHHHHCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
QRQIKKELVSHRHAGEPLTVMEAFNERNEAEKVADRIRTMRMSGTNDYRSFAIFYRTNAQ
HHHHHHHHHHHHCCCCCHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCH
SRVLEDIMRQQRIPYRLFGSVSFYKRKEIKDAVAYLRFIVNERDSESLLRIINFPPRKIG
HHHHHHHHHHHCCCEEECCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCC
DVSIAKLRDFAEVRHISLYEAIHRAAEAGFPARLLNALASFTSVIEALREMATRGTVYDV
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
LNELFTLTSIPLLLQAENTPESLARHENLQELLSMARDFADHNPDGGSLGDFLENISLAS
HHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCHHC
DYDETQESDNYVSLMTVHASKGLEFPVVFITGLEERLFPLHTYEPEELEEERRLFYVAMT
CCCHHHCCCCEEEEEEEECCCCCCCCEEEEECCHHHCCCCCCCCHHHHHHHHHEEHHHHH
RAQEKIFLSYAKSRYQYGQLHQSIASTFISEIDASIVQSEGGRLLSDRRAPREATTPQNH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCCCCCCCCCCC
AAAPAMRRPTTSGMAPSSASSPTAESSSPSISNGTLVHHPLFGQGVVLEVQGKGSKQKVR
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCEEEEEECCCCCCEEE
IAFRNAGEKTLMVQYANLKIQTS
EEEECCCCEEEEEEEECEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8232203 [H]