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The map label for this gene is leuA2 [H]

Identifier: 78189195

GI number: 78189195

Start: 1631078

End: 1632223

Strand: Reverse

Name: leuA2 [H]

Synonym: Cag_1229

Alternate gene names: 78189195

Gene position: 1632223-1631078 (Counterclockwise)

Preceding gene: 78189197

Following gene: 78189194

Centisome position: 63.46

GC content: 49.74

Gene sequence:

>1146_bases
ATGCCAAATTCTGCAATGGAGCTGCGCAAATCGTGGATTATTGATACCACCTTGCGCGATGGCGAGCAAGCCCCTGGTGT
GGTGTTTAGTGCGGAGGAGAAGCGCGATATTGCAGCGCAACTTGCGGCAGCAGGTGTTAGTGAATTGGAGGTTGGTTACC
CCGCCATTAGTGGCGATGAGTTAGAAACCATCCGCTCAATTGTTGCTATGCGTCTGCCTTTGCGTGTAACAAGCTGGGCG
CGTGCAAAGTGGGATGATATTGAGGCTGCTCGCCAAAGTGGCACCGAAGCGGTTCATATTAGTTTTCCTGTATCGGCGCT
TTATTTGCAATTAATGGAGCGCTCTTACGAGTGGGTGCAGGAGCAGTTAAGCGAATTAATCGGCAAAGCCAAAGATTATT
TCGAGTTTGTGAGTGTTGGGGCGCAAGATGCCACCCGTGCGGATATTGAGCTGCTTTCGCGCTTTGTTTGTGATGCAAGC
GCGGCAGGCGCTCAGCGCATTCGCCTTGCCGATACGGTGGGAATTGCCACGCCAATTTCCGTGATGCACCTTATTGGGGA
ACTGCAACGAGTTACTTCAGTGGATCTTGAATTTCATGCTCATAACGATCTTGGTATGGCTACAGCAAATGCGTTTACAG
CCCTTGCTGTTGGTTGCCAAGCCGTTAGTGTGTCGGTAACGGGGCTTGGCGAACGGGCGGGCAACGCTGCGCTTGAAGAG
CTTGCAATTGCTTTGAAACTTTCGGGAGAGTTTGAAGCCACCATAAAAACTGAAATGTTGTCGAGCTTATGCGAAACGGT
AAGCAAAGCGGCTGGTAGGGTGATTGATGAGCGCAAAGCCGTGATTGGCAAAGCTGTTTTTCAGCACGAATCGGGCATTC
ACTGTGCCGCATTGTTGAAGCATCCGCTCTCTTATCAGCCATTTTTACCCGAACAAATTGGCGGTAGAGAGCATGAATTG
GTGATTGGCAAGCATTCGGGAAGTGCGGCTATTCAGCACTTTTTTGCCGAGCGAGGCATTCCGCTGAGCCGCAGCGAGGC
AACACAGTTGTTAGCAAAGGTTCGCCAAATGGCGACTGAAAAAAAAGGATTGCTTACAGCTAAAGAACTTGAAGAGCTTT
ATACAGAACTGTTTAATATTCATTGA

Upstream 100 bases:

>100_bases
TTAAAAAGATTTAATTTCCTTAAATAATGAGGAATTAACGGCTTTTAACCTCAATTCCTCCATTTTTGTTATGACGCACG
TTTTAGCCATATCGCCATCT

Downstream 100 bases:

>100_bases
AATTGCATTTTATCTGATTTTTTGTGTTTTGATTTTTTGCGAAAAAAGTTTTCTCTCTTAGCATTACCCAAAGAGATACA
ACGTTGCAACCAACTTGTTG

Product: homocitrate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase 2; Alpha-isopropylmalate synthase 2 [H]

Number of amino acids: Translated: 381; Mature: 380

Protein sequence:

>381_residues
MPNSAMELRKSWIIDTTLRDGEQAPGVVFSAEEKRDIAAQLAAAGVSELEVGYPAISGDELETIRSIVAMRLPLRVTSWA
RAKWDDIEAARQSGTEAVHISFPVSALYLQLMERSYEWVQEQLSELIGKAKDYFEFVSVGAQDATRADIELLSRFVCDAS
AAGAQRIRLADTVGIATPISVMHLIGELQRVTSVDLEFHAHNDLGMATANAFTALAVGCQAVSVSVTGLGERAGNAALEE
LAIALKLSGEFEATIKTEMLSSLCETVSKAAGRVIDERKAVIGKAVFQHESGIHCAALLKHPLSYQPFLPEQIGGREHEL
VIGKHSGSAAIQHFFAERGIPLSRSEATQLLAKVRQMATEKKGLLTAKELEELYTELFNIH

Sequences:

>Translated_381_residues
MPNSAMELRKSWIIDTTLRDGEQAPGVVFSAEEKRDIAAQLAAAGVSELEVGYPAISGDELETIRSIVAMRLPLRVTSWA
RAKWDDIEAARQSGTEAVHISFPVSALYLQLMERSYEWVQEQLSELIGKAKDYFEFVSVGAQDATRADIELLSRFVCDAS
AAGAQRIRLADTVGIATPISVMHLIGELQRVTSVDLEFHAHNDLGMATANAFTALAVGCQAVSVSVTGLGERAGNAALEE
LAIALKLSGEFEATIKTEMLSSLCETVSKAAGRVIDERKAVIGKAVFQHESGIHCAALLKHPLSYQPFLPEQIGGREHEL
VIGKHSGSAAIQHFFAERGIPLSRSEATQLLAKVRQMATEKKGLLTAKELEELYTELFNIH
>Mature_380_residues
PNSAMELRKSWIIDTTLRDGEQAPGVVFSAEEKRDIAAQLAAAGVSELEVGYPAISGDELETIRSIVAMRLPLRVTSWAR
AKWDDIEAARQSGTEAVHISFPVSALYLQLMERSYEWVQEQLSELIGKAKDYFEFVSVGAQDATRADIELLSRFVCDASA
AGAQRIRLADTVGIATPISVMHLIGELQRVTSVDLEFHAHNDLGMATANAFTALAVGCQAVSVSVTGLGERAGNAALEEL
AIALKLSGEFEATIKTEMLSSLCETVSKAAGRVIDERKAVIGKAVFQHESGIHCAALLKHPLSYQPFLPEQIGGREHELV
IGKHSGSAAIQHFFAERGIPLSRSEATQLLAKVRQMATEKKGLLTAKELEELYTELFNIH

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786261, Length=373, Percent_Identity=33.5120643431635, Blast_Score=178, Evalue=5e-46,
Organism=Saccharomyces cerevisiae, GI6320071, Length=363, Percent_Identity=28.099173553719, Blast_Score=148, Evalue=2e-36,
Organism=Saccharomyces cerevisiae, GI6320019, Length=372, Percent_Identity=27.1505376344086, Blast_Score=147, Evalue=3e-36,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR013477
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like [H]

EC number: =2.3.3.13 [H]

Molecular weight: Translated: 41383; Mature: 41251

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1 ; PS00816 AIPM_HOMOCIT_SYNTH_2 ; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPNSAMELRKSWIIDTTLRDGEQAPGVVFSAEEKRDIAAQLAAAGVSELEVGYPAISGDE
CCCHHHHHHHHHHEECHHCCCCCCCCEEEECHHHHHHHHHHHHCCHHHHHCCCCCCCCHH
LETIRSIVAMRLPLRVTSWARAKWDDIEAARQSGTEAVHISFPVSALYLQLMERSYEWVQ
HHHHHHHHHHHCCHHHHHHHHCCHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHH
EQLSELIGKAKDYFEFVSVGAQDATRADIELLSRFVCDASAAGAQRIRLADTVGIATPIS
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCHHEEHHHHHCCHHHHH
VMHLIGELQRVTSVDLEFHAHNDLGMATANAFTALAVGCQAVSVSVTGLGERAGNAALEE
HHHHHHHHHHHHHCCEEEECCCCCCHHHHHHHHHHHHHHHEEEEEEEECCCCCCHHHHHH
LAIALKLSGEFEATIKTEMLSSLCETVSKAAGRVIDERKAVIGKAVFQHESGIHCAALLK
HHHHHEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
HPLSYQPFLPEQIGGREHELVIGKHSGSAAIQHFFAERGIPLSRSEATQLLAKVRQMATE
CCCCCCCCCHHHHCCCCCEEEEECCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHH
KKGLLTAKELEELYTELFNIH
HHCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure 
PNSAMELRKSWIIDTTLRDGEQAPGVVFSAEEKRDIAAQLAAAGVSELEVGYPAISGDE
CCHHHHHHHHHHEECHHCCCCCCCCEEEECHHHHHHHHHHHHCCHHHHHCCCCCCCCHH
LETIRSIVAMRLPLRVTSWARAKWDDIEAARQSGTEAVHISFPVSALYLQLMERSYEWVQ
HHHHHHHHHHHCCHHHHHHHHCCHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHH
EQLSELIGKAKDYFEFVSVGAQDATRADIELLSRFVCDASAAGAQRIRLADTVGIATPIS
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCHHEEHHHHHCCHHHHH
VMHLIGELQRVTSVDLEFHAHNDLGMATANAFTALAVGCQAVSVSVTGLGERAGNAALEE
HHHHHHHHHHHHHCCEEEECCCCCCHHHHHHHHHHHHHHHEEEEEEEECCCCCCHHHHHH
LAIALKLSGEFEATIKTEMLSSLCETVSKAAGRVIDERKAVIGKAVFQHESGIHCAALLK
HHHHHEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
HPLSYQPFLPEQIGGREHELVIGKHSGSAAIQHFFAERGIPLSRSEATQLLAKVRQMATE
CCCCCCCCCHHHHCCCCCEEEEECCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHH
KKGLLTAKELEELYTELFNIH
HHCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11997336 [H]