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The map label for this gene is vnfA [H]

Identifier: 78189194

GI number: 78189194

Start: 1629314

End: 1630963

Strand: Reverse

Name: vnfA [H]

Synonym: Cag_1228

Alternate gene names: 78189194

Gene position: 1630963-1629314 (Counterclockwise)

Preceding gene: 78189195

Following gene: 78189191

Centisome position: 63.41

GC content: 46.67

Gene sequence:

>1650_bases
ATGCTTATTACACAACAAAACGACTACGGCAGCATCAGTCTTTTAGCTGAAGTAAGCCGCACCATTACGCATGAAGAGGA
CATTAACAAAGTGTTACGCTTAGTGCTCTTTATTATGTCGGAAAACATGCACATGCAGCGAGGTATGATTACCATTCTTA
ACCGCAGCACGGGCGAAATTGTTATTAACGAATCGTTTGGGCTAACCGAAGAGGAGCGTGAGCGTGGACGCTATCACATT
GGCGAGGGTGTTATTGGGCATGTTGTAAAAACCGGCAAAGCCGTTATTGTGCCCTCCATTCAAGATGAACCGCTTTTTCT
TGATAGAACAGGATCGCGTGCTCAAGCAAAAAAAGAGGAACTCTGCTTTATTTGCATTCCGATTAAAGCAGGCTCCGAAA
TTATTGGTACCCTCAGTGCCGACCGCCACGTAGAACCCGTTACTGCCGATGAGCATCGCCGCAAAACACGCCAAACCGAC
GACCGTGATGAGCGGATTGATAAACTCCAATTTTATGTGGATCAGCTCTCCATTATTGCCGCCATGATTTCGCAAGCCGT
GCGCTTAAAGCAGCTTGCCTACGAAGCAGGCAGCAAAAACGTGCAAGATTTGAGCAACTTGCCACCTTTTGCAGCCTCCA
TTCCACCCCGCAGCGAAGATCGCCAAGTTAACGCCATTCCGCCGCCATCGCCCGATCGCCCAGCAAACATTATTGGCAAC
ACCAAGCCAATGGTTTCGCTTTATTCCATGATTGATAAAATTGCCAAAACCAGTGCCACCACGCTTGTGCTTGGCGAAAG
CGGCGTTGGTAAAGAGTTAGTAGCAAGCGCCATCCACTTTAAAAGCCGCCGCGCTGAAAAGCCCTTTATTAAATTTAACT
GTGCAGCCCTTCCCGAAAACATTGTAGAGAGCGAACTTTTTGGGCACGAAAAAGGAGCTTTTACAGGTGCCCTTGCTACA
CGGCATGGACGCTTTGAAATGGCAAACAACGGCACTATTTTTCTTGATGAAGTGGGCGAACTCAGCTTATCAGTTCAAGC
GAAATTGTTGAGAATTTTGCAAGAAAAGGAGTTTGAGCGCGTTGGCGGCTCAAAAACCATTAAGGTAGATGTTCGTGTTA
TTGCTGCCACCAACCGTAACCTCGAAGAGCTTATTCGGCAGGGGCAGTTTCGCGAAGATTTATTCTATCGCCTCAACATT
TTCCCCATTACCGTACCACCCTTGCGCGAGCGTAAAACCGATATTTTGCTGCTTGCTGACTATTTTGTTGAGAAGTATAA
CAAAGCCAATCAGAAAGGAGTACGCCGAATTTCCACCACAGCCATTGATATGCTTATGCGCTACCATTGGCCTGGTAACG
TGCGCGAATTGCAAAACTGTATTGAGCGTGCGGTTATTTTAAGTGAGGATAATGTGATTCACGGCTACCACTTGCCGCCA
ACCTTGCAAACGGCGGAATCAAGCGGTACGCCTTACACGGGCTCATTGCAACAAAAGCTTGATGCTATTGAAAAGGAAAT
GATTATTGAAGCGCTTAAACGCACGCAAGGCAATATGTCGCGTGCTGCCATGCAGCTTGGATTATCGGAGCGTATTATGG
GATTGCGCATTAAAAAGTTTAATATTGATTATAGGAAGTTTAGGGTGTGA

Upstream 100 bases:

>100_bases
TGATTTTTTGTGTTTTGATTTTTTGCGAAAAAAGTTTTCTCTCTTAGCATTACCCAAAGAGATACAACGTTGCAACCAAC
TTGTTGAAACCATTACGTTC

Downstream 100 bases:

>100_bases
GAGGGGGGGGCAATGCGATTGTTCACATTGTTTCCCCTAATTGCTCCAAACGAATGAGCCATTCATGAAAATGACGGCAT
TTTTTTCGCAAAATGGAAAG

Product: Fis family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 549; Mature: 549

Protein sequence:

>549_residues
MLITQQNDYGSISLLAEVSRTITHEEDINKVLRLVLFIMSENMHMQRGMITILNRSTGEIVINESFGLTEEERERGRYHI
GEGVIGHVVKTGKAVIVPSIQDEPLFLDRTGSRAQAKKEELCFICIPIKAGSEIIGTLSADRHVEPVTADEHRRKTRQTD
DRDERIDKLQFYVDQLSIIAAMISQAVRLKQLAYEAGSKNVQDLSNLPPFAASIPPRSEDRQVNAIPPPSPDRPANIIGN
TKPMVSLYSMIDKIAKTSATTLVLGESGVGKELVASAIHFKSRRAEKPFIKFNCAALPENIVESELFGHEKGAFTGALAT
RHGRFEMANNGTIFLDEVGELSLSVQAKLLRILQEKEFERVGGSKTIKVDVRVIAATNRNLEELIRQGQFREDLFYRLNI
FPITVPPLRERKTDILLLADYFVEKYNKANQKGVRRISTTAIDMLMRYHWPGNVRELQNCIERAVILSEDNVIHGYHLPP
TLQTAESSGTPYTGSLQQKLDAIEKEMIIEALKRTQGNMSRAAMQLGLSERIMGLRIKKFNIDYRKFRV

Sequences:

>Translated_549_residues
MLITQQNDYGSISLLAEVSRTITHEEDINKVLRLVLFIMSENMHMQRGMITILNRSTGEIVINESFGLTEEERERGRYHI
GEGVIGHVVKTGKAVIVPSIQDEPLFLDRTGSRAQAKKEELCFICIPIKAGSEIIGTLSADRHVEPVTADEHRRKTRQTD
DRDERIDKLQFYVDQLSIIAAMISQAVRLKQLAYEAGSKNVQDLSNLPPFAASIPPRSEDRQVNAIPPPSPDRPANIIGN
TKPMVSLYSMIDKIAKTSATTLVLGESGVGKELVASAIHFKSRRAEKPFIKFNCAALPENIVESELFGHEKGAFTGALAT
RHGRFEMANNGTIFLDEVGELSLSVQAKLLRILQEKEFERVGGSKTIKVDVRVIAATNRNLEELIRQGQFREDLFYRLNI
FPITVPPLRERKTDILLLADYFVEKYNKANQKGVRRISTTAIDMLMRYHWPGNVRELQNCIERAVILSEDNVIHGYHLPP
TLQTAESSGTPYTGSLQQKLDAIEKEMIIEALKRTQGNMSRAAMQLGLSERIMGLRIKKFNIDYRKFRV
>Mature_549_residues
MLITQQNDYGSISLLAEVSRTITHEEDINKVLRLVLFIMSENMHMQRGMITILNRSTGEIVINESFGLTEEERERGRYHI
GEGVIGHVVKTGKAVIVPSIQDEPLFLDRTGSRAQAKKEELCFICIPIKAGSEIIGTLSADRHVEPVTADEHRRKTRQTD
DRDERIDKLQFYVDQLSIIAAMISQAVRLKQLAYEAGSKNVQDLSNLPPFAASIPPRSEDRQVNAIPPPSPDRPANIIGN
TKPMVSLYSMIDKIAKTSATTLVLGESGVGKELVASAIHFKSRRAEKPFIKFNCAALPENIVESELFGHEKGAFTGALAT
RHGRFEMANNGTIFLDEVGELSLSVQAKLLRILQEKEFERVGGSKTIKVDVRVIAATNRNLEELIRQGQFREDLFYRLNI
FPITVPPLRERKTDILLLADYFVEKYNKANQKGVRRISTTAIDMLMRYHWPGNVRELQNCIERAVILSEDNVIHGYHLPP
TLQTAESSGTPYTGSLQQKLDAIEKEMIIEALKRTQGNMSRAAMQLGLSERIMGLRIKKFNIDYRKFRV

Specific function: Required for the expression of the V-dependent nitrogen fixation system in Azotobacter vinelandii. It is required for the regulation of nitrogenase 2 transcription. Interacts with sigma- 54 [H]

COG id: COG3604

COG function: function code KT; Transcriptional regulator containing GAF, AAA-type ATPase, and DNA binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=315, Percent_Identity=49.8412698412698, Blast_Score=333, Evalue=2e-92,
Organism=Escherichia coli, GI87082117, Length=328, Percent_Identity=45.7317073170732, Blast_Score=293, Evalue=2e-80,
Organism=Escherichia coli, GI1790437, Length=318, Percent_Identity=45.9119496855346, Blast_Score=287, Evalue=1e-78,
Organism=Escherichia coli, GI87082152, Length=303, Percent_Identity=48.1848184818482, Blast_Score=274, Evalue=1e-74,
Organism=Escherichia coli, GI1789087, Length=310, Percent_Identity=45.8064516129032, Blast_Score=273, Evalue=3e-74,
Organism=Escherichia coli, GI1789233, Length=328, Percent_Identity=43.2926829268293, Blast_Score=257, Evalue=1e-69,
Organism=Escherichia coli, GI1790299, Length=331, Percent_Identity=39.2749244712991, Blast_Score=249, Evalue=3e-67,
Organism=Escherichia coli, GI1788905, Length=312, Percent_Identity=38.7820512820513, Blast_Score=232, Evalue=4e-62,
Organism=Escherichia coli, GI87081872, Length=319, Percent_Identity=38.871473354232, Blast_Score=225, Evalue=5e-60,
Organism=Escherichia coli, GI1786524, Length=326, Percent_Identity=40.7975460122699, Blast_Score=218, Evalue=6e-58,
Organism=Escherichia coli, GI1787583, Length=321, Percent_Identity=38.3177570093458, Blast_Score=215, Evalue=7e-57,
Organism=Escherichia coli, GI87081858, Length=303, Percent_Identity=32.013201320132, Blast_Score=150, Evalue=2e-37,
Organism=Escherichia coli, GI1789828, Length=232, Percent_Identity=38.3620689655172, Blast_Score=149, Evalue=4e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR003018
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078 [H]

Pfam domain/function: PF01590 GAF; PF02954 HTH_8; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 61864; Mature: 61864

Theoretical pI: Translated: 8.98; Mature: 8.98

Prosite motif: PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLITQQNDYGSISLLAEVSRTITHEEDINKVLRLVLFIMSENMHMQRGMITILNRSTGEI
CEEECCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHCCEEEEEECCCCCE
VINESFGLTEEERERGRYHIGEGVIGHVVKTGKAVIVPSIQDEPLFLDRTGSRAQAKKEE
EEECCCCCCHHHHHCCCEECCCCHHHHHHHCCCEEEEECCCCCCEEEECCCCHHHHHCCC
LCFICIPIKAGSEIIGTLSADRHVEPVTADEHRRKTRQTDDRDERIDKLQFYVDQLSIIA
EEEEEEEECCCCHHEEECCCCCCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
AMISQAVRLKQLAYEAGSKNVQDLSNLPPFAASIPPRSEDRQVNAIPPPSPDRPANIIGN
HHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCCCCCCCCEECCCCCCCCCCCCCCCCC
TKPMVSLYSMIDKIAKTSATTLVLGESGVGKELVASAIHFKSRRAEKPFIKFNCAALPEN
CCHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCEEEEEHHHCCHH
IVESELFGHEKGAFTGALATRHGRFEMANNGTIFLDEVGELSLSVQAKLLRILQEKEFER
HHHHHHHCCCCCCEEHHHHHCCCCEEECCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHH
VGGSKTIKVDVRVIAATNRNLEELIRQGQFREDLFYRLNIFPITVPPLRERKTDILLLAD
CCCCEEEEEEEEEEEECCCCHHHHHHCCCCHHHHEEEEEEEEEECCCCCCCCCCEEEEHH
YFVEKYNKANQKGVRRISTTAIDMLMRYHWPGNVRELQNCIERAVILSEDNVIHGYHLPP
HHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHEEECCCCEEEEEECCC
TLQTAESSGTPYTGSLQQKLDAIEKEMIIEALKRTQGNMSRAAMQLGLSERIMGLRIKKF
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCHHHHHHCEEEEEE
NIDYRKFRV
CCCHHHCCC
>Mature Secondary Structure
MLITQQNDYGSISLLAEVSRTITHEEDINKVLRLVLFIMSENMHMQRGMITILNRSTGEI
CEEECCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHCCEEEEEECCCCCE
VINESFGLTEEERERGRYHIGEGVIGHVVKTGKAVIVPSIQDEPLFLDRTGSRAQAKKEE
EEECCCCCCHHHHHCCCEECCCCHHHHHHHCCCEEEEECCCCCCEEEECCCCHHHHHCCC
LCFICIPIKAGSEIIGTLSADRHVEPVTADEHRRKTRQTDDRDERIDKLQFYVDQLSIIA
EEEEEEEECCCCHHEEECCCCCCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
AMISQAVRLKQLAYEAGSKNVQDLSNLPPFAASIPPRSEDRQVNAIPPPSPDRPANIIGN
HHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCCCCCCCCEECCCCCCCCCCCCCCCCC
TKPMVSLYSMIDKIAKTSATTLVLGESGVGKELVASAIHFKSRRAEKPFIKFNCAALPEN
CCHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCEEEEEHHHCCHH
IVESELFGHEKGAFTGALATRHGRFEMANNGTIFLDEVGELSLSVQAKLLRILQEKEFER
HHHHHHHCCCCCCEEHHHHHCCCCEEECCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHH
VGGSKTIKVDVRVIAATNRNLEELIRQGQFREDLFYRLNIFPITVPPLRERKTDILLLAD
CCCCEEEEEEEEEEEECCCCHHHHHHCCCCHHHHEEEEEEEEEECCCCCCCCCCEEEEHH
YFVEKYNKANQKGVRRISTTAIDMLMRYHWPGNVRELQNCIERAVILSEDNVIHGYHLPP
HHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHEEECCCCEEEEEECCC
TLQTAESSGTPYTGSLQQKLDAIEKEMIIEALKRTQGNMSRAAMQLGLSERIMGLRIKKF
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCHHHHHHCEEEEEE
NIDYRKFRV
CCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2722750 [H]