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The map label for this gene is pth [H]

Identifier: 78189056

GI number: 78189056

Start: 1470466

End: 1471119

Strand: Reverse

Name: pth [H]

Synonym: Cag_1089

Alternate gene names: 78189056

Gene position: 1471119-1470466 (Counterclockwise)

Preceding gene: 78189059

Following gene: 78189052

Centisome position: 57.2

GC content: 46.18

Gene sequence:

>654_bases
ATGTTACATTGCCGCCATGCTTGCAAACAACAAAAGCGTGTGAAAATTATTTTACCATTAACCTCCTTTTTTTCGATAGT
TATGAAACTGATTATAGGGCTTGGCAACCCCGGCACTGAATATGATGGCACTCGCCACAACATTGGCTTTGCCGTTGCCG
ATGCTCTTGCCGCTCACCACAACGCCTCTTTCAGCAAAGAAAAAGGGCGCTACCTCTCCGCCAAAATTAAGCTTGGTAGC
GAATCTGTCGGCATTATTAAGCCCATGACCTACATGAACCACTCAGGGCATGCCGTTGTTGCTGCCATGAATTTCTATAA
AGTTGCTCGCACGGAAATTGTGGTGATATGCGACGATCTGAATTTACCGGTGGGCACCATGCGTTTGCGCCCAAAAGGAT
CGGCTGGTGGGCAGAATGGCTTAAAGCATATTATTGAATGCTTTGGCAGCAACGAATTTGCTCGCCTTCGTATTGGCATT
GGTAGTCAAAACATGCCAAAAGGCGGGTTCTCATCATTTGTATTGGGGAAATTTAGTGAGCAAGAAAAAAGCGAAATAGC
CATTATGACCGTTTCGGCGCGTGATTGCGCCATTGATTTTGCGCTTCACGGTTTAGGGCACGCCATGAACCACTTTAACA
CCTCAAAGCTGTAA

Upstream 100 bases:

>100_bases
TTTTGTGAAAAAGAGTCTCAACCAAAAGAGGCTCTATAAAGCGGCGTAATCTGCACTATTTACAGCTCACATCAACCCGC
TTTGGGGAGAGTTTGCCGAG

Downstream 100 bases:

>100_bases
AGCAGCTCCATTAACTATGCAGCTTAATAAAGCGAATTGCTCGTTGGTTAAACTCACGAGCTTGATCCACATTGCACACA
TGCCCTGAATTAGCAATTAC

Product: peptidyl-tRNA hydrolase

Products: NA

Alternate protein names: PTH [H]

Number of amino acids: Translated: 217; Mature: 217

Protein sequence:

>217_residues
MLHCRHACKQQKRVKIILPLTSFFSIVMKLIIGLGNPGTEYDGTRHNIGFAVADALAAHHNASFSKEKGRYLSAKIKLGS
ESVGIIKPMTYMNHSGHAVVAAMNFYKVARTEIVVICDDLNLPVGTMRLRPKGSAGGQNGLKHIIECFGSNEFARLRIGI
GSQNMPKGGFSSFVLGKFSEQEKSEIAIMTVSARDCAIDFALHGLGHAMNHFNTSKL

Sequences:

>Translated_217_residues
MLHCRHACKQQKRVKIILPLTSFFSIVMKLIIGLGNPGTEYDGTRHNIGFAVADALAAHHNASFSKEKGRYLSAKIKLGS
ESVGIIKPMTYMNHSGHAVVAAMNFYKVARTEIVVICDDLNLPVGTMRLRPKGSAGGQNGLKHIIECFGSNEFARLRIGI
GSQNMPKGGFSSFVLGKFSEQEKSEIAIMTVSARDCAIDFALHGLGHAMNHFNTSKL
>Mature_217_residues
MLHCRHACKQQKRVKIILPLTSFFSIVMKLIIGLGNPGTEYDGTRHNIGFAVADALAAHHNASFSKEKGRYLSAKIKLGS
ESVGIIKPMTYMNHSGHAVVAAMNFYKVARTEIVVICDDLNLPVGTMRLRPKGSAGGQNGLKHIIECFGSNEFARLRIGI
GSQNMPKGGFSSFVLGKFSEQEKSEIAIMTVSARDCAIDFALHGLGHAMNHFNTSKL

Specific function: The natural substrate for this enzyme may be peptidyl- tRNAs which drop off the ribosome during protein synthesis [H]

COG id: COG0193

COG function: function code J; Peptidyl-tRNA hydrolase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PTH family [H]

Homologues:

Organism=Homo sapiens, GI50897284, Length=173, Percent_Identity=33.5260115606936, Blast_Score=102, Evalue=2e-22,
Organism=Escherichia coli, GI1787455, Length=193, Percent_Identity=39.3782383419689, Blast_Score=109, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6321983, Length=167, Percent_Identity=32.9341317365269, Blast_Score=72, Evalue=9e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001328
- InterPro:   IPR018171 [H]

Pfam domain/function: PF01195 Pept_tRNA_hydro [H]

EC number: =3.1.1.29 [H]

Molecular weight: Translated: 23631; Mature: 23631

Theoretical pI: Translated: 10.16; Mature: 10.16

Prosite motif: PS01195 PEPT_TRNA_HYDROL_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
6.5 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
6.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLHCRHACKQQKRVKIILPLTSFFSIVMKLIIGLGNPGTEYDGTRHNIGFAVADALAAHH
CCCCHHHHHHCCCEEEEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHC
NASFSKEKGRYLSAKIKLGSESVGIIKPMTYMNHSGHAVVAAMNFYKVARTEIVVICDDL
CCCCCCCCCCEEEEEEEECCCCCCEEECHHHCCCCCCEEEEEHHHHHEECEEEEEEEECC
NLPVGTMRLRPKGSAGGQNGLKHIIECFGSNEFARLRIGIGSQNMPKGGFSSFVLGKFSE
CCCCCEEEECCCCCCCCHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCHHHHCCCCC
QEKSEIAIMTVSARDCAIDFALHGLGHAMNHFNTSKL
CCCCEEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MLHCRHACKQQKRVKIILPLTSFFSIVMKLIIGLGNPGTEYDGTRHNIGFAVADALAAHH
CCCCHHHHHHCCCEEEEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHC
NASFSKEKGRYLSAKIKLGSESVGIIKPMTYMNHSGHAVVAAMNFYKVARTEIVVICDDL
CCCCCCCCCCEEEEEEEECCCCCCEEECHHHCCCCCCEEEEEHHHHHEECEEEEEEEECC
NLPVGTMRLRPKGSAGGQNGLKHIIECFGSNEFARLRIGIGSQNMPKGGFSSFVLGKFSE
CCCCCEEEECCCCCCCCHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCHHHHCCCCC
QEKSEIAIMTVSARDCAIDFALHGLGHAMNHFNTSKL
CCCCEEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA