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The map label for this gene is 78188910

Identifier: 78188910

GI number: 78188910

Start: 1278328

End: 1279212

Strand: Reverse

Name: 78188910

Synonym: Cag_0942

Alternate gene names: NA

Gene position: 1279212-1278328 (Counterclockwise)

Preceding gene: 78188911

Following gene: 78188909

Centisome position: 49.73

GC content: 47.68

Gene sequence:

>885_bases
ATGAGCAAAGAATCTGTTTCAATTGCTGTGGTGCAAAGCGAGTGCAAGGGCGACGCAGTAGCAAACCGTGCCGAAGCCAC
AGCAAAAATTCGAGAAGCTGCCGCACTTGGTGCACAAATCATCTGCTTGCAAGAGCTGTTTGTAACCCGCTATTTTTGCC
AAACCGAAGCCTATGAGCCGTTTGGTGAGGCAGAAGCAATTCCCGATGGAGCAACTACTCGCCTTATGCAAGAGCTTGCG
GCGGAGCTTGGGGTAGTAATTATTGCCTCCTTGTTTGAACGCCGTGCACGCGGCTTACACCACAACACCGCAGTAGTTAT
TGATGCCGATGGCAGCTACCTTGGCATGTATCGTAAAATGCACATTCCCGACGATCCGGGTTTTTATGAAAAGTTTTACT
TCACACCGAGCGACCTTGGTTACAAGGTTTTTAAAACGCGCTATGCCACCATTGGCGTGCTTATTTGTTGGGATCAATGG
TACCCTGAAGCTGCTCGTTTAACAGCCTTGAAAGGGGCAGAAATTCTTTTTTACCCAACAGCAATTGGTTGGGCAACCGA
TGAAGATTCAGCCGAAGTTCGCCATGCGCAGCAAAATGCGTGGATTACCATGCAGCGCAGCCATGCTATTGCAAATGGTG
TTTTTGTAGCCGCCGCAAACCGCGTTGGCACAGAGGAAAATCTTGAATTTTGGGGCAACAGTTTTATAAGTGATCCTTTT
GGTCAAATGGTTGCCGAAGCGCCCCATCAACATGAAACAATTCTGCTTGCCCAATGCGATTTGAGCCGTATTAACTTTTA
CCGTTCGCATTGGCCCTTTTTGCGCGACCGCCGCATTGAAACCTATGGCGGTTTACAACAACGTTTTCTTGATAATAATC
AATAA

Upstream 100 bases:

>100_bases
GTTAGCGGGTACAGGTCAAGTGATGACGTTGCACGGTATGTTGCTATCCATCGGATTGTTGGTGCCTTAAGGGCATCTTT
AAAAATTGAGTTATAGACAA

Downstream 100 bases:

>100_bases
TGAACTGTAGAAACAACGTATGCGTTGTCTCTACAATCATGGAATTAAAATTTTCATCCTACGATAGTTTACTCTATGAA
TATTTTTGGTGTTGCTATTT

Product: carbon-nitrogen hydrolase family protein

Products: NA

Alternate protein names: D-N-alpha-carbamilase [H]

Number of amino acids: Translated: 294; Mature: 293

Protein sequence:

>294_residues
MSKESVSIAVVQSECKGDAVANRAEATAKIREAAALGAQIICLQELFVTRYFCQTEAYEPFGEAEAIPDGATTRLMQELA
AELGVVIIASLFERRARGLHHNTAVVIDADGSYLGMYRKMHIPDDPGFYEKFYFTPSDLGYKVFKTRYATIGVLICWDQW
YPEAARLTALKGAEILFYPTAIGWATDEDSAEVRHAQQNAWITMQRSHAIANGVFVAAANRVGTEENLEFWGNSFISDPF
GQMVAEAPHQHETILLAQCDLSRINFYRSHWPFLRDRRIETYGGLQQRFLDNNQ

Sequences:

>Translated_294_residues
MSKESVSIAVVQSECKGDAVANRAEATAKIREAAALGAQIICLQELFVTRYFCQTEAYEPFGEAEAIPDGATTRLMQELA
AELGVVIIASLFERRARGLHHNTAVVIDADGSYLGMYRKMHIPDDPGFYEKFYFTPSDLGYKVFKTRYATIGVLICWDQW
YPEAARLTALKGAEILFYPTAIGWATDEDSAEVRHAQQNAWITMQRSHAIANGVFVAAANRVGTEENLEFWGNSFISDPF
GQMVAEAPHQHETILLAQCDLSRINFYRSHWPFLRDRRIETYGGLQQRFLDNNQ
>Mature_293_residues
SKESVSIAVVQSECKGDAVANRAEATAKIREAAALGAQIICLQELFVTRYFCQTEAYEPFGEAEAIPDGATTRLMQELAA
ELGVVIIASLFERRARGLHHNTAVVIDADGSYLGMYRKMHIPDDPGFYEKFYFTPSDLGYKVFKTRYATIGVLICWDQWY
PEAARLTALKGAEILFYPTAIGWATDEDSAEVRHAQQNAWITMQRSHAIANGVFVAAANRVGTEENLEFWGNSFISDPFG
QMVAEAPHQHETILLAQCDLSRINFYRSHWPFLRDRRIETYGGLQQRFLDNNQ

Specific function: The enzyme catalyzes the hydrolysis of N-carbamoyl-D- amino acids to the corresponding which are useful intermediates in the preparation of beta-lactam antibiotics. Industrial production of beta-lactam antibiotics is now being developed using this enzyme

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Homo sapiens, GI7706509, Length=305, Percent_Identity=31.4754098360656, Blast_Score=126, Evalue=2e-29,
Organism=Homo sapiens, GI9910460, Length=285, Percent_Identity=31.2280701754386, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI5031947, Length=298, Percent_Identity=28.8590604026846, Blast_Score=96, Evalue=4e-20,
Organism=Homo sapiens, GI297632348, Length=298, Percent_Identity=28.8590604026846, Blast_Score=96, Evalue=5e-20,
Organism=Homo sapiens, GI297632350, Length=298, Percent_Identity=28.8590604026846, Blast_Score=96, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI17533173, Length=285, Percent_Identity=34.3859649122807, Blast_Score=135, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI17556280, Length=201, Percent_Identity=28.8557213930348, Blast_Score=75, Evalue=5e-14,
Organism=Saccharomyces cerevisiae, GI6323383, Length=295, Percent_Identity=26.1016949152542, Blast_Score=100, Evalue=4e-22,
Organism=Drosophila melanogaster, GI21358471, Length=292, Percent_Identity=31.5068493150685, Blast_Score=132, Evalue=2e-31,
Organism=Drosophila melanogaster, GI21355835, Length=292, Percent_Identity=27.3972602739726, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI17933642, Length=289, Percent_Identity=26.2975778546713, Blast_Score=82, Evalue=4e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003010 [H]

Pfam domain/function: PF00795 CN_hydrolase [H]

EC number: =3.5.1.77 [H]

Molecular weight: Translated: 33104; Mature: 32972

Theoretical pI: Translated: 5.20; Mature: 5.20

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKESVSIAVVQSECKGDAVANRAEATAKIREAAALGAQIICLQELFVTRYFCQTEAYEP
CCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
FGEAEAIPDGATTRLMQELAAELGVVIIASLFERRARGLHHNTAVVIDADGSYLGMYRKM
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCEEEEEEEE
HIPDDPGFYEKFYFTPSDLGYKVFKTRYATIGVLICWDQWYPEAARLTALKGAEILFYPT
CCCCCCCCCCEEEECCCCCCHHHHHHHHEEEEEEEEECCCCCCHHHEEEECCCEEEEEEC
AIGWATDEDSAEVRHAQQNAWITMQRSHAIANGVFVAAANRVGTEENLEFWGNSFISDPF
CCCCCCCCCHHHHHHHHCCCEEEEECCHHHHCCEEEEECCCCCCCCCHHHHCCCCCCCHH
GQMVAEAPHQHETILLAQCDLSRINFYRSHWPFLRDRRIETYGGLQQRFLDNNQ
HHHHHCCCCCCCEEEEEECCHHHHHHHHHCCCHHHCCCCHHHCCHHHHHCCCCC
>Mature Secondary Structure 
SKESVSIAVVQSECKGDAVANRAEATAKIREAAALGAQIICLQELFVTRYFCQTEAYEP
CCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
FGEAEAIPDGATTRLMQELAAELGVVIIASLFERRARGLHHNTAVVIDADGSYLGMYRKM
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCEEEEEEEE
HIPDDPGFYEKFYFTPSDLGYKVFKTRYATIGVLICWDQWYPEAARLTALKGAEILFYPT
CCCCCCCCCCEEEECCCCCCHHHHHHHHEEEEEEEEECCCCCCHHHEEEECCCEEEEEEC
AIGWATDEDSAEVRHAQQNAWITMQRSHAIANGVFVAAANRVGTEENLEFWGNSFISDPF
CCCCCCCCCHHHHHHHHCCCEEEEECCHHHHCCEEEEECCCCCCCCCHHHHCCCCCCCHH
GQMVAEAPHQHETILLAQCDLSRINFYRSHWPFLRDRRIETYGGLQQRFLDNNQ
HHHHHCCCCCCCEEEEEECCHHHHHHHHHCCCHHHCCCCHHHCCHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9648217; 10903946 [H]