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The map label for this gene is pfkA

Identifier: 78188849

GI number: 78188849

Start: 1211927

End: 1212979

Strand: Reverse

Name: pfkA

Synonym: Cag_0879

Alternate gene names: 78188849

Gene position: 1212979-1211927 (Counterclockwise)

Preceding gene: 78188850

Following gene: 78188848

Centisome position: 47.16

GC content: 43.02

Gene sequence:

>1053_bases
TTGTTGTTGGAGGACACTCGTAGCAACACATTAAAACTAACATTTGGCGGTTTTGTGAATAAAAAAGCATTAGAAAAAAT
AGCGGTATTAACCTCAGGTGGCGATGCGCCAGGTATGAATGCCGCTATTCGTGCGGTTACTCGCGCAGCAATTAGCAATA
AATTGAAAGTTGTAGGTATTCGTCGCGGCTATCAAGGAATGATAGAGGGTGATTTTATTAACCTTAAAGCCTCCGATGTT
AGCGGTATTCTGCAACTTGGTGGCACCATGCTTAAAACTGCCCGTAGCGCGGCATTTCGCACAACGGAAGGGCGAGCACA
AGCACACGAGCAATTAAGCAAAGCCGCAATTGATGCAGTTGTAGTTATTGGTGGCGATGGTTCATTTCATGGCGCATTAA
TGATGTCGCATGAATATAATATCCCGTTTGTAGGTATTCCCGCCACCATTGATAACGATATGTATGGCACCGATTACACT
ATTGGCTACGAAACAGCGCTTAATACAGTAGTTGAAGCCGTTGATAAAATTAGAGAAACTGCCCGCTCACACGGGCGTGT
TTTTTTTGTTGAAGTAATGGGGCATGAAGCTGGTATGATTGCTCTCAATAGTAGCATAGCGTGTGGTGCTGAAGTGGTTG
TTATTCCCGAATTACACAACCGTCAATATGATGAGTTACATAAATTTTTATGCAAAGGATATAAAAAGAAAGAGAGTAGT
GGTATTGTTATTGTTGCAGAAGGCGAAGAAACAGGAGGTGCTCTTAAGATTGCCGAACAGGTACGTAAAGAGCATCCCGA
AATAGATGTTCGCGTTTCAATTCTTGGGCACATTCAGCGTGGTGGCAGCCCAGCCGCAAAAGATCGCATTAATGCAACGC
GAATGGGGGCAGCCGCAATTGAAGCGCTGCTTGATGATCAAAAAAGCGTGATGATTGGTTTAGCTAACGATCAAATTGTT
CGCGTTCCTTTTAATAAAGCGGTTAAAACAAATCGCACCATTAGCCATGATTTGTTGGAAATGCAACGATTGATGAATAT
GTGGTGCTGTTAA

Upstream 100 bases:

>100_bases
ACAATACTATAAAAAATAGTGAACAAAAAAGTTGAAAGAAGGGCTGATGAGCATTAAAGCAGTATTCGCTTTTTTGTACT
CAATTTTAAAATAATGAGAA

Downstream 100 bases:

>100_bases
AGCATTTTTGTTCTATTGAGCCACGAAGGTAAACAATGAGAATTAAATCACTTTCATATCTTGATTCCCATTGGGAATTG
CAAAACCTTGAGTTAAGCAA

Product: 6-phosphofructokinase

Products: NA

Alternate protein names: Phosphofructokinase 2; Phosphohexokinase 2 [H]

Number of amino acids: Translated: 350; Mature: 350

Protein sequence:

>350_residues
MLLEDTRSNTLKLTFGGFVNKKALEKIAVLTSGGDAPGMNAAIRAVTRAAISNKLKVVGIRRGYQGMIEGDFINLKASDV
SGILQLGGTMLKTARSAAFRTTEGRAQAHEQLSKAAIDAVVVIGGDGSFHGALMMSHEYNIPFVGIPATIDNDMYGTDYT
IGYETALNTVVEAVDKIRETARSHGRVFFVEVMGHEAGMIALNSSIACGAEVVVIPELHNRQYDELHKFLCKGYKKKESS
GIVIVAEGEETGGALKIAEQVRKEHPEIDVRVSILGHIQRGGSPAAKDRINATRMGAAAIEALLDDQKSVMIGLANDQIV
RVPFNKAVKTNRTISHDLLEMQRLMNMWCC

Sequences:

>Translated_350_residues
MLLEDTRSNTLKLTFGGFVNKKALEKIAVLTSGGDAPGMNAAIRAVTRAAISNKLKVVGIRRGYQGMIEGDFINLKASDV
SGILQLGGTMLKTARSAAFRTTEGRAQAHEQLSKAAIDAVVVIGGDGSFHGALMMSHEYNIPFVGIPATIDNDMYGTDYT
IGYETALNTVVEAVDKIRETARSHGRVFFVEVMGHEAGMIALNSSIACGAEVVVIPELHNRQYDELHKFLCKGYKKKESS
GIVIVAEGEETGGALKIAEQVRKEHPEIDVRVSILGHIQRGGSPAAKDRINATRMGAAAIEALLDDQKSVMIGLANDQIV
RVPFNKAVKTNRTISHDLLEMQRLMNMWCC
>Mature_350_residues
MLLEDTRSNTLKLTFGGFVNKKALEKIAVLTSGGDAPGMNAAIRAVTRAAISNKLKVVGIRRGYQGMIEGDFINLKASDV
SGILQLGGTMLKTARSAAFRTTEGRAQAHEQLSKAAIDAVVVIGGDGSFHGALMMSHEYNIPFVGIPATIDNDMYGTDYT
IGYETALNTVVEAVDKIRETARSHGRVFFVEVMGHEAGMIALNSSIACGAEVVVIPELHNRQYDELHKFLCKGYKKKESS
GIVIVAEGEETGGALKIAEQVRKEHPEIDVRVSILGHIQRGGSPAAKDRINATRMGAAAIEALLDDQKSVMIGLANDQIV
RVPFNKAVKTNRTISHDLLEMQRLMNMWCC

Specific function: Key control step of glycolysis. [C]

COG id: COG0205

COG function: function code G; 6-phosphofructokinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphofructokinase family [H]

Homologues:

Organism=Homo sapiens, GI266453768, Length=367, Percent_Identity=38.4196185286104, Blast_Score=223, Evalue=1e-58,
Organism=Homo sapiens, GI266453748, Length=367, Percent_Identity=38.4196185286104, Blast_Score=223, Evalue=1e-58,
Organism=Homo sapiens, GI4505749, Length=367, Percent_Identity=38.4196185286104, Blast_Score=223, Evalue=1e-58,
Organism=Homo sapiens, GI266453619, Length=367, Percent_Identity=38.4196185286104, Blast_Score=223, Evalue=2e-58,
Organism=Homo sapiens, GI11321601, Length=348, Percent_Identity=40.2298850574713, Blast_Score=222, Evalue=5e-58,
Organism=Homo sapiens, GI48762920, Length=344, Percent_Identity=39.2441860465116, Blast_Score=215, Evalue=5e-56,
Organism=Escherichia coli, GI1790350, Length=324, Percent_Identity=45.3703703703704, Blast_Score=248, Evalue=3e-67,
Organism=Caenorhabditis elegans, GI25147584, Length=343, Percent_Identity=39.9416909620991, Blast_Score=216, Evalue=1e-56,
Organism=Caenorhabditis elegans, GI17558788, Length=364, Percent_Identity=33.5164835164835, Blast_Score=186, Evalue=1e-47,
Organism=Caenorhabditis elegans, GI72003601, Length=351, Percent_Identity=29.6296296296296, Blast_Score=141, Evalue=4e-34,
Organism=Saccharomyces cerevisiae, GI6321679, Length=362, Percent_Identity=40.6077348066298, Blast_Score=230, Evalue=2e-61,
Organism=Saccharomyces cerevisiae, GI6323861, Length=358, Percent_Identity=34.6368715083799, Blast_Score=196, Evalue=5e-51,
Organism=Drosophila melanogaster, GI24652337, Length=349, Percent_Identity=40.974212034384, Blast_Score=224, Evalue=5e-59,
Organism=Drosophila melanogaster, GI17647809, Length=349, Percent_Identity=40.974212034384, Blast_Score=224, Evalue=6e-59,
Organism=Drosophila melanogaster, GI28573326, Length=351, Percent_Identity=40.7407407407407, Blast_Score=218, Evalue=4e-57,

Paralogues:

None

Copy number: 3981 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 950 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012003
- InterPro:   IPR012828
- InterPro:   IPR022953
- InterPro:   IPR015912
- InterPro:   IPR000023 [H]

Pfam domain/function: PF00365 PFK [H]

EC number: =2.7.1.11 [H]

Molecular weight: Translated: 37869; Mature: 37869

Theoretical pI: Translated: 8.02; Mature: 8.02

Prosite motif: PS00433 PHOSPHOFRUCTOKINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLEDTRSNTLKLTFGGFVNKKALEKIAVLTSGGDAPGMNAAIRAVTRAAISNKLKVVGI
CCCCCCCCCEEEEEECCCCCHHHHHHEEEEECCCCCCCCHHHHHHHHHHHHHCCEEEEEE
RRGYQGMIEGDFINLKASDVSGILQLGGTMLKTARSAAFRTTEGRAQAHEQLSKAAIDAV
ECCCCCCCCCCEEEEEHHHHHHHHHHCHHHHHHHHHHHEECCCCHHHHHHHHHHHHEEEE
VVIGGDGSFHGALMMSHEYNIPFVGIPATIDNDMYGTDYTIGYETALNTVVEAVDKIRET
EEECCCCCCCEEEEEEECCCCCEEECCCEECCCCCCCCEEEHHHHHHHHHHHHHHHHHHH
ARSHGRVFFVEVMGHEAGMIALNSSIACGAEVVVIPELHNRQYDELHKFLCKGYKKKESS
HHHCCCEEEEEEECCCCCEEEECCCCCCCEEEEEECCCCCCCHHHHHHHHHCCCCCCCCC
GIVIVAEGEETGGALKIAEQVRKEHPEIDVRVSILGHIQRGGSPAAKDRINATRMGAAAI
CEEEEECCCCCCCHHHHHHHHHHHCCCEEEEEEEEEHHHCCCCCCHHHCCCHHHHHHHHH
EALLDDQKSVMIGLANDQIVRVPFNKAVKTNRTISHDLLEMQRLMNMWCC
HHHHCCCCEEEEEECCCCEEEECCCHHHHCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLLEDTRSNTLKLTFGGFVNKKALEKIAVLTSGGDAPGMNAAIRAVTRAAISNKLKVVGI
CCCCCCCCCEEEEEECCCCCHHHHHHEEEEECCCCCCCCHHHHHHHHHHHHHCCEEEEEE
RRGYQGMIEGDFINLKASDVSGILQLGGTMLKTARSAAFRTTEGRAQAHEQLSKAAIDAV
ECCCCCCCCCCEEEEEHHHHHHHHHHCHHHHHHHHHHHEECCCCHHHHHHHHHHHHEEEE
VVIGGDGSFHGALMMSHEYNIPFVGIPATIDNDMYGTDYTIGYETALNTVVEAVDKIRET
EEECCCCCCCEEEEEEECCCCCEEECCCEECCCCCCCCEEEHHHHHHHHHHHHHHHHHHH
ARSHGRVFFVEVMGHEAGMIALNSSIACGAEVVVIPELHNRQYDELHKFLCKGYKKKESS
HHHCCCEEEEEEECCCCCEEEECCCCCCCEEEEEECCCCCCCHHHHHHHHHCCCCCCCCC
GIVIVAEGEETGGALKIAEQVRKEHPEIDVRVSILGHIQRGGSPAAKDRINATRMGAAAI
CEEEEECCCCCCCHHHHHHHHHHHCCCEEEEEEEEEHHHCCCCCCHHHCCCHHHHHHHHH
EALLDDQKSVMIGLANDQIVRVPFNKAVKTNRTISHDLLEMQRLMNMWCC
HHHHCCCCEEEEEECCCCEEEECCCHHHHCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12663928 [H]