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The map label for this gene is mdh

Identifier: 78188832

GI number: 78188832

Start: 1195530

End: 1196462

Strand: Reverse

Name: mdh

Synonym: Cag_0862

Alternate gene names: 78188832

Gene position: 1196462-1195530 (Counterclockwise)

Preceding gene: 78188833

Following gene: 78188830

Centisome position: 46.52

GC content: 41.26

Gene sequence:

>933_bases
ATGAAAATTACCGTTATTGGAGCAGGAAATGTAGGAGCAACTGCTGCTCTTAAAATTGCTGAAAAACAGTTTGCTAATGA
GGTGGTTTTAATTGATGTGGTTGAAGGTATTCCACAAGGCAAAGCGCTTGATATGTATGAATCTGGTGCCGTATCGTTGT
TTGATACAAGGGTTATCGGTTCCAATGACTATAAAGATTCAGCAGATTCAGATATTATTTTAATTACCGCTGGTTTAGCT
CGTAAACCGGGTATGACGCGCGAAGATTTGCTGATGAAAAATGCAGCAATCATTAAAGAAGTTACCTCGCAGGTAATGAA
GTACTCTACCAACCCGATTATTGTTATGGTCTCCAATCCTGTGGATATTATGACCTACGTGGCGCATCGGGTAAGTGGGT
TACCAAAAGAGCGGGTTATTGGTATGGGTGGTGTGCTTGATACGGCACGCTATAAAAATTTTATTGCCGAAACGCTTAAT
ATTTCGATGCAAGATATTAGTGCACTTGTGCTTGGTGGGCATGGTGATGCAATGGTTCCCGTTGTTAATTATACCAATGT
AGCAGGTATTCCGCTTACCGAATTATTACCGTTGGATATTATTGATGGCTTGGTAGAACGTACTCGGAATGGTGGTATTG
AAATTGTAAACTACCTTAAATCAGGCTCTGCATATTATGCGCCAGCCGCATCAACGGTTGAAATGATTGAGGCAATTGCT
CGCGATCGTAAGCGCATTCTGCCTTGTACTACATTGCTTGATGGGCAATATGGCATTAACAGTGTTTTTTGTGGTGTGCC
AGTAAAGTTAGGCAAGAATGGTATTGAGCAGGTGTTGGAAATTAATCTTTCTGCTCCCGAGCGTTCAGCATTGCAACGTT
CAGCCGATATTGTAGAGAAGAATTGCAAAATGCTTGAGAGTTTGTTTGCATAA

Upstream 100 bases:

>100_bases
CGTAGCCATTACGTTCTGCAAAGCTATTTTTGCATAGTTAATGTGAATCTCCTTCACATTCCGTTATTTTTTTCTCTCAC
CTTAAATCAATGTCGGACTT

Downstream 100 bases:

>100_bases
GCTTTTTTGGTACAGAGATTTTTTTATAAAAGGCACTCAATCGGGTGCCTTTTTTTGTGCGCTTCGTATTAAAGTACAAT
CTTGGTGGTTAATGTTCTCT

Product: malate dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 310; Mature: 310

Protein sequence:

>310_residues
MKITVIGAGNVGATAALKIAEKQFANEVVLIDVVEGIPQGKALDMYESGAVSLFDTRVIGSNDYKDSADSDIILITAGLA
RKPGMTREDLLMKNAAIIKEVTSQVMKYSTNPIIVMVSNPVDIMTYVAHRVSGLPKERVIGMGGVLDTARYKNFIAETLN
ISMQDISALVLGGHGDAMVPVVNYTNVAGIPLTELLPLDIIDGLVERTRNGGIEIVNYLKSGSAYYAPAASTVEMIEAIA
RDRKRILPCTTLLDGQYGINSVFCGVPVKLGKNGIEQVLEINLSAPERSALQRSADIVEKNCKMLESLFA

Sequences:

>Translated_310_residues
MKITVIGAGNVGATAALKIAEKQFANEVVLIDVVEGIPQGKALDMYESGAVSLFDTRVIGSNDYKDSADSDIILITAGLA
RKPGMTREDLLMKNAAIIKEVTSQVMKYSTNPIIVMVSNPVDIMTYVAHRVSGLPKERVIGMGGVLDTARYKNFIAETLN
ISMQDISALVLGGHGDAMVPVVNYTNVAGIPLTELLPLDIIDGLVERTRNGGIEIVNYLKSGSAYYAPAASTVEMIEAIA
RDRKRILPCTTLLDGQYGINSVFCGVPVKLGKNGIEQVLEINLSAPERSALQRSADIVEKNCKMLESLFA
>Mature_310_residues
MKITVIGAGNVGATAALKIAEKQFANEVVLIDVVEGIPQGKALDMYESGAVSLFDTRVIGSNDYKDSADSDIILITAGLA
RKPGMTREDLLMKNAAIIKEVTSQVMKYSTNPIIVMVSNPVDIMTYVAHRVSGLPKERVIGMGGVLDTARYKNFIAETLN
ISMQDISALVLGGHGDAMVPVVNYTNVAGIPLTELLPLDIIDGLVERTRNGGIEIVNYLKSGSAYYAPAASTVEMIEAIA
RDRKRILPCTTLLDGQYGINSVFCGVPVKLGKNGIEQVLEINLSAPERSALQRSADIVEKNCKMLESLFA

Specific function: Catalyzes the reversible oxidation of malate to oxaloacetate

COG id: COG0039

COG function: function code C; Malate/lactate dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the LDH/MDH superfamily. MDH type 3 family

Homologues:

Organism=Homo sapiens, GI47059044, Length=303, Percent_Identity=37.953795379538, Blast_Score=210, Evalue=1e-54,
Organism=Homo sapiens, GI221136809, Length=303, Percent_Identity=37.953795379538, Blast_Score=210, Evalue=1e-54,
Organism=Homo sapiens, GI5031857, Length=302, Percent_Identity=37.7483443708609, Blast_Score=202, Evalue=3e-52,
Organism=Homo sapiens, GI260099723, Length=302, Percent_Identity=37.7483443708609, Blast_Score=202, Evalue=4e-52,
Organism=Homo sapiens, GI291575128, Length=315, Percent_Identity=36.5079365079365, Blast_Score=197, Evalue=7e-51,
Organism=Homo sapiens, GI4557032, Length=315, Percent_Identity=36.5079365079365, Blast_Score=197, Evalue=7e-51,
Organism=Homo sapiens, GI15082234, Length=300, Percent_Identity=36, Blast_Score=192, Evalue=3e-49,
Organism=Homo sapiens, GI9257228, Length=303, Percent_Identity=34.6534653465347, Blast_Score=191, Evalue=1e-48,
Organism=Homo sapiens, GI4504973, Length=303, Percent_Identity=34.6534653465347, Blast_Score=191, Evalue=1e-48,
Organism=Homo sapiens, GI260099725, Length=196, Percent_Identity=41.8367346938776, Blast_Score=160, Evalue=2e-39,
Organism=Homo sapiens, GI260099727, Length=196, Percent_Identity=41.8367346938776, Blast_Score=159, Evalue=3e-39,
Organism=Homo sapiens, GI207028494, Length=188, Percent_Identity=38.2978723404255, Blast_Score=125, Evalue=6e-29,
Organism=Homo sapiens, GI103472011, Length=285, Percent_Identity=27.3684210526316, Blast_Score=104, Evalue=9e-23,
Organism=Homo sapiens, GI21735621, Length=298, Percent_Identity=25.8389261744966, Blast_Score=82, Evalue=7e-16,
Organism=Homo sapiens, GI103472015, Length=179, Percent_Identity=30.1675977653631, Blast_Score=81, Evalue=1e-15,
Organism=Escherichia coli, GI1789632, Length=328, Percent_Identity=30.4878048780488, Blast_Score=103, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI17535107, Length=293, Percent_Identity=33.4470989761092, Blast_Score=160, Evalue=1e-39,
Organism=Caenorhabditis elegans, GI17554310, Length=312, Percent_Identity=28.525641025641, Blast_Score=92, Evalue=4e-19,
Organism=Saccharomyces cerevisiae, GI6322765, Length=294, Percent_Identity=28.9115646258503, Blast_Score=86, Evalue=7e-18,
Organism=Saccharomyces cerevisiae, GI6320125, Length=336, Percent_Identity=27.3809523809524, Blast_Score=78, Evalue=2e-15,
Organism=Drosophila melanogaster, GI17136226, Length=305, Percent_Identity=34.4262295081967, Blast_Score=185, Evalue=4e-47,
Organism=Drosophila melanogaster, GI45550422, Length=319, Percent_Identity=30.4075235109718, Blast_Score=147, Evalue=6e-36,
Organism=Drosophila melanogaster, GI24647881, Length=332, Percent_Identity=29.2168674698795, Blast_Score=100, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24663595, Length=261, Percent_Identity=26.8199233716475, Blast_Score=82, Evalue=6e-16,
Organism=Drosophila melanogaster, GI24663599, Length=310, Percent_Identity=25.8064516129032, Blast_Score=81, Evalue=8e-16,

Paralogues:

None

Copy number: 2640 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2380 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase

Swissprot (AC and ID): MDH_CHLCH (Q3AS98)

Other databases:

- EMBL:   CP000108
- RefSeq:   YP_379170.1
- ProteinModelPortal:   Q3AS98
- SMR:   Q3AS98
- STRING:   Q3AS98
- GeneID:   3747572
- GenomeReviews:   CP000108_GR
- KEGG:   cch:Cag_0862
- eggNOG:   COG0039
- HOGENOM:   HBG566126
- OMA:   AMTYTVF
- ProtClustDB:   PRK06223
- BioCyc:   CCHL340177:CAG_0862-MONOMER
- GO:   GO:0005488
- GO:   GO:0006096
- HAMAP:   MF_00487
- InterPro:   IPR001557
- InterPro:   IPR022383
- InterPro:   IPR001236
- InterPro:   IPR015955
- InterPro:   IPR011275
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.90.110.10
- Gene3D:   G3DSA:3.40.50.720
- PIRSF:   PIRSF000102
- PRINTS:   PR00086
- TIGRFAMs:   TIGR01763

Pfam domain/function: PF02866 Ldh_1_C; PF00056 Ldh_1_N; SSF56327 Lactate_DH/Glyco_hydro_4_C

EC number: =1.1.1.37

Molecular weight: Translated: 33276; Mature: 33276

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: NA

Important sites: ACT_SITE 174-174 BINDING 32-32 BINDING 81-81 BINDING 87-87 BINDING 94-94 BINDING 119-119 BINDING 150-150

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKITVIGAGNVGATAALKIAEKQFANEVVLIDVVEGIPQGKALDMYESGAVSLFDTRVIG
CEEEEEECCCCCCHHHHHHHHHHHCCCEEEEEECCCCCCCCEECHHCCCCEEEEEEEEEC
SNDYKDSADSDIILITAGLARKPGMTREDLLMKNAAIIKEVTSQVMKYSTNPIIVMVSNP
CCCCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCC
VDIMTYVAHRVSGLPKERVIGMGGVLDTARYKNFIAETLNISMQDISALVLGGHGDAMVP
HHHHHHHHHHHCCCCHHHEEECCCHHHHHHHHHHHHHHHCCCHHHEEEEEECCCCCEEEE
VVNYTNVAGIPLTELLPLDIIDGLVERTRNGGIEIVNYLKSGSAYYAPAASTVEMIEAIA
EEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCEEECCCHHHHHHHHHHH
RDRKRILPCTTLLDGQYGINSVFCGVPVKLGKNGIEQVLEINLSAPERSALQRSADIVEK
HHHHHCCCHHHEECCCCCCCEEEECCCHHHCHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
NCKMLESLFA
HHHHHHHHCC
>Mature Secondary Structure
MKITVIGAGNVGATAALKIAEKQFANEVVLIDVVEGIPQGKALDMYESGAVSLFDTRVIG
CEEEEEECCCCCCHHHHHHHHHHHCCCEEEEEECCCCCCCCEECHHCCCCEEEEEEEEEC
SNDYKDSADSDIILITAGLARKPGMTREDLLMKNAAIIKEVTSQVMKYSTNPIIVMVSNP
CCCCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCC
VDIMTYVAHRVSGLPKERVIGMGGVLDTARYKNFIAETLNISMQDISALVLGGHGDAMVP
HHHHHHHHHHHCCCCHHHEEECCCHHHHHHHHHHHHHHHCCCHHHEEEEEECCCCCEEEE
VVNYTNVAGIPLTELLPLDIIDGLVERTRNGGIEIVNYLKSGSAYYAPAASTVEMIEAIA
EEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCEEECCCHHHHHHHHHHH
RDRKRILPCTTLLDGQYGINSVFCGVPVKLGKNGIEQVLEINLSAPERSALQRSADIVEK
HHHHHCCCHHHEECCCCCCCEEEECCCHHHCHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
NCKMLESLFA
HHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA