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The map label for this gene is fumA [H]

Identifier: 78188830

GI number: 78188830

Start: 1192034

End: 1193554

Strand: Reverse

Name: fumA [H]

Synonym: Cag_0860

Alternate gene names: 78188830

Gene position: 1193554-1192034 (Counterclockwise)

Preceding gene: 78188832

Following gene: 78188829

Centisome position: 46.4

GC content: 47.6

Gene sequence:

>1521_bases
ATGGAACAATTTAAAGCTTCAATGCTTGCGCTTATTACCGAAACTTCAGCGAATCTGCCAAGCGACATTCGCCGTGCTCT
TGCGGCGGCTATTCAGCGTGAAGATGCGGCTTCGCAAGCTGGGCTTGCCATGTCAACCATTTCGGTAAATATTGATATGG
CAGTTGACAATATTTCGCCCGTCTGCCAAGATACAGGAATGCCAACCTTTTTTATTCACATCCCAAAAGGGGTGGATATG
CTTCCATTGAAGCGTGATATTGAGGAAGCTATTGCAGAGGCATCAAAAACAGGGAAACTTCGTCCAAATGCGGTTGATGC
AATCAGTGGTAAAAATTCGGGCAACAACCTCGGCGAGCATGTGCCCGTTATTCATTTTGAGCCTTGGGATAAGGATGAAA
TTGAAGTAAAGCTCATTTTAAAAGGTGGTGGATGTGAGAATAAGAATATTCAATACTCATTGCCGCAAGAAATTCCCGGA
CTTGGTACTGCTTCGCGCGATTTGGAGGGAGTGCGCAAATGCTTGCTCCATGCAGTATATCAAGCACAAGGGCAGGGATG
TAGCCCCGGCGTGATTGGCGTTGGCGTTGGTGGCGATCGTACCAGCGGTTTTGAGCTTGCTAAAAAGCAGCTTTTCCGTA
TGCTCGACGACCGTAATCCCAACAGTGAGCTGGATGCGCTTGAGCAGGAGATTATGGAAAAAGCCAATAACATGAATATT
GGACCCATGGGTTTTGGTGGCAAAACAACGCTGCTGGGCTGCAAAATTGGTATGTCGCACCGTGTGCCAGCAAGCTTTTT
TGTTTCAGTAGCCTATAACTGCTGGGCATACCGTCGTCTTGGCGTTCTCATTGATCCTGCAACGGGAGCAATTACCCATT
GGCACTATCGTGAGGCGGATGAAATTAAGCGTATGGCACAAGGCGAGGGAATGCCGCTTACGGGACGTGAAGTTGTGTTG
CAAGCGCCTGTAACCGAAGAGCAAGTGCGCAGCTTAAAGGTTGGTGATGTGGTGCTGGTTAATGGCACCATGCACACAGG
GCGCGACGCTTTTCACCACTACATTATGCACCACGATTTACCCGCTGGGCTTGATACAAACGGAGGTATTTTGTTCCATT
GCGGTCCCGTCATTATGAAAAACGAGGATGGCAGTTACCGCGTAACCGCAGCAGGTCCAACAACCTCCATTCGCGAAGAG
CCATTCCAAGCCGATGTTATTAAAAAGCTTGGTTTGCGCGTTGTGATTGGTAAAGGCGGTATGGGTCCAAAAACTTTGGC
TGGCTTACAAGAGCATGGCGCAGTTTACTTGAACGCTATTGGCGGCGCTGCTCAATATTATGCACGTTGCATTGAAAAAG
TTACAGGCGTTGATTTTCTTGAAGAGATGGGCGTTCCTGAAGCCATGTGGCATTTTGAAGCAAATTCATTCCCTGCCATT
GTAACAATGGATGCACATGGCAATAGTTTGCATCAGCAAATTGAAGAGGAGTCGTTTAACGCTCTTGCTTCTTTTAAATG
A

Upstream 100 bases:

>100_bases
TGTGGCATAGAATGGAATCCTTTAAAAAAAATGTAAATTCGCCCACGCTTTAGCCTTCCTTGCTCTCCATTTTCTTTTCA
ATTTTACCCATTCACCATTT

Downstream 100 bases:

>100_bases
TGGATTGTAGGGGCGGAATCCATTTCCGCCCTGACGAAGGGCAGATATAGAATCTGCCCCTACGACGGAATGGTTTTATT
GATTATGGATGTTGAATTTT

Product: Fe-S type hydro-lyase tartrate/fumarate alpha and beta region

Products: NA

Alternate protein names: Fumarase [H]

Number of amino acids: Translated: 506; Mature: 506

Protein sequence:

>506_residues
MEQFKASMLALITETSANLPSDIRRALAAAIQREDAASQAGLAMSTISVNIDMAVDNISPVCQDTGMPTFFIHIPKGVDM
LPLKRDIEEAIAEASKTGKLRPNAVDAISGKNSGNNLGEHVPVIHFEPWDKDEIEVKLILKGGGCENKNIQYSLPQEIPG
LGTASRDLEGVRKCLLHAVYQAQGQGCSPGVIGVGVGGDRTSGFELAKKQLFRMLDDRNPNSELDALEQEIMEKANNMNI
GPMGFGGKTTLLGCKIGMSHRVPASFFVSVAYNCWAYRRLGVLIDPATGAITHWHYREADEIKRMAQGEGMPLTGREVVL
QAPVTEEQVRSLKVGDVVLVNGTMHTGRDAFHHYIMHHDLPAGLDTNGGILFHCGPVIMKNEDGSYRVTAAGPTTSIREE
PFQADVIKKLGLRVVIGKGGMGPKTLAGLQEHGAVYLNAIGGAAQYYARCIEKVTGVDFLEEMGVPEAMWHFEANSFPAI
VTMDAHGNSLHQQIEEESFNALASFK

Sequences:

>Translated_506_residues
MEQFKASMLALITETSANLPSDIRRALAAAIQREDAASQAGLAMSTISVNIDMAVDNISPVCQDTGMPTFFIHIPKGVDM
LPLKRDIEEAIAEASKTGKLRPNAVDAISGKNSGNNLGEHVPVIHFEPWDKDEIEVKLILKGGGCENKNIQYSLPQEIPG
LGTASRDLEGVRKCLLHAVYQAQGQGCSPGVIGVGVGGDRTSGFELAKKQLFRMLDDRNPNSELDALEQEIMEKANNMNI
GPMGFGGKTTLLGCKIGMSHRVPASFFVSVAYNCWAYRRLGVLIDPATGAITHWHYREADEIKRMAQGEGMPLTGREVVL
QAPVTEEQVRSLKVGDVVLVNGTMHTGRDAFHHYIMHHDLPAGLDTNGGILFHCGPVIMKNEDGSYRVTAAGPTTSIREE
PFQADVIKKLGLRVVIGKGGMGPKTLAGLQEHGAVYLNAIGGAAQYYARCIEKVTGVDFLEEMGVPEAMWHFEANSFPAI
VTMDAHGNSLHQQIEEESFNALASFK
>Mature_506_residues
MEQFKASMLALITETSANLPSDIRRALAAAIQREDAASQAGLAMSTISVNIDMAVDNISPVCQDTGMPTFFIHIPKGVDM
LPLKRDIEEAIAEASKTGKLRPNAVDAISGKNSGNNLGEHVPVIHFEPWDKDEIEVKLILKGGGCENKNIQYSLPQEIPG
LGTASRDLEGVRKCLLHAVYQAQGQGCSPGVIGVGVGGDRTSGFELAKKQLFRMLDDRNPNSELDALEQEIMEKANNMNI
GPMGFGGKTTLLGCKIGMSHRVPASFFVSVAYNCWAYRRLGVLIDPATGAITHWHYREADEIKRMAQGEGMPLTGREVVL
QAPVTEEQVRSLKVGDVVLVNGTMHTGRDAFHHYIMHHDLPAGLDTNGGILFHCGPVIMKNEDGSYRVTAAGPTTSIREE
PFQADVIKKLGLRVVIGKGGMGPKTLAGLQEHGAVYLNAIGGAAQYYARCIEKVTGVDFLEEMGVPEAMWHFEANSFPAI
VTMDAHGNSLHQQIEEESFNALASFK

Specific function: Unknown

COG id: COG1951

COG function: function code C; Tartrate dehydratase alpha subunit/Fumarate hydratase class I, N-terminal domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I fumarase family [H]

Homologues:

Organism=Escherichia coli, GI1789443, Length=173, Percent_Identity=37.5722543352601, Blast_Score=130, Evalue=2e-31,
Organism=Escherichia coli, GI1789442, Length=286, Percent_Identity=29.020979020979, Blast_Score=121, Evalue=9e-29,
Organism=Escherichia coli, GI1787897, Length=477, Percent_Identity=26.6247379454927, Blast_Score=104, Evalue=1e-23,
Organism=Escherichia coli, GI1790564, Length=498, Percent_Identity=24.4979919678715, Blast_Score=95, Evalue=9e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004646
- InterPro:   IPR004647
- InterPro:   IPR011167 [H]

Pfam domain/function: PF05681 Fumerase; PF05683 Fumerase_C [H]

EC number: =4.2.1.2 [H]

Molecular weight: Translated: 54787; Mature: 54787

Theoretical pI: Translated: 5.95; Mature: 5.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEQFKASMLALITETSANLPSDIRRALAAAIQREDAASQAGLAMSTISVNIDMAVDNISP
CCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCEEEEEEEEEEEEEECCCCH
VCQDTGMPTFFIHIPKGVDMLPLKRDIEEAIAEASKTGKLRPNAVDAISGKNSGNNLGEH
HHHCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHCCCCCCCCCCCCC
VPVIHFEPWDKDEIEVKLILKGGGCENKNIQYSLPQEIPGLGTASRDLEGVRKCLLHAVY
CCEEEECCCCCCCEEEEEEEECCCCCCCCEEEECHHHCCCCCCCHHHHHHHHHHHHHHHH
QAQGQGCSPGVIGVGVGGDRTSGFELAKKQLFRMLDDRNPNSELDALEQEIMEKANNMNI
HHCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCC
GPMGFGGKTTLLGCKIGMSHRVPASFFVSVAYNCWAYRRLGVLIDPATGAITHWHYREAD
CCCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCEEECCCCCHH
EIKRMAQGEGMPLTGREVVLQAPVTEEQVRSLKVGDVVLVNGTMHTGRDAFHHYIMHHDL
HHHHHHCCCCCCCCCCEEEEECCCCHHHHHHEECCCEEEEECCCCCCHHHHHHHHHHCCC
PAGLDTNGGILFHCGPVIMKNEDGSYRVTAAGPTTSIREEPFQADVIKKLGLRVVIGKGG
CCCCCCCCCEEEEECCEEEECCCCCEEEEECCCCCCHHCCCHHHHHHHHCCCEEEEECCC
MGPKTLAGLQEHGAVYLNAIGGAAQYYARCIEKVTGVDFLEEMGVPEAMWHFEANSFPAI
CCHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHCHHHHHHCCCCHHHEEECCCCCCEE
VTMDAHGNSLHQQIEEESFNALASFK
EEECCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MEQFKASMLALITETSANLPSDIRRALAAAIQREDAASQAGLAMSTISVNIDMAVDNISP
CCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCEEEEEEEEEEEEEECCCCH
VCQDTGMPTFFIHIPKGVDMLPLKRDIEEAIAEASKTGKLRPNAVDAISGKNSGNNLGEH
HHHCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHCCCCCCCCCCCCC
VPVIHFEPWDKDEIEVKLILKGGGCENKNIQYSLPQEIPGLGTASRDLEGVRKCLLHAVY
CCEEEECCCCCCCEEEEEEEECCCCCCCCEEEECHHHCCCCCCCHHHHHHHHHHHHHHHH
QAQGQGCSPGVIGVGVGGDRTSGFELAKKQLFRMLDDRNPNSELDALEQEIMEKANNMNI
HHCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCC
GPMGFGGKTTLLGCKIGMSHRVPASFFVSVAYNCWAYRRLGVLIDPATGAITHWHYREAD
CCCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCEEECCCCCHH
EIKRMAQGEGMPLTGREVVLQAPVTEEQVRSLKVGDVVLVNGTMHTGRDAFHHYIMHHDL
HHHHHHCCCCCCCCCCEEEEECCCCHHHHHHEECCCEEEEECCCCCCHHHHHHHHHHCCC
PAGLDTNGGILFHCGPVIMKNEDGSYRVTAAGPTTSIREEPFQADVIKKLGLRVVIGKGG
CCCCCCCCCEEEEECCEEEECCCCCEEEEECCCCCCHHCCCHHHHHHHHCCCEEEEECCC
MGPKTLAGLQEHGAVYLNAIGGAAQYYARCIEKVTGVDFLEEMGVPEAMWHFEANSFPAI
CCHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHCHHHHHHCCCCHHHEEECCCCCCEE
VTMDAHGNSLHQQIEEESFNALASFK
EEECCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8473853 [H]