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The map label for this gene is trmD

Identifier: 78188773

GI number: 78188773

Start: 1126066

End: 1126761

Strand: Reverse

Name: trmD

Synonym: Cag_0802

Alternate gene names: 78188773

Gene position: 1126761-1126066 (Counterclockwise)

Preceding gene: 78188774

Following gene: 78188772

Centisome position: 43.81

GC content: 44.4

Gene sequence:

>696_bases
ATGCGTTTTGATGTAATTTCTGTTATCCCATATTTTTTTGATTCAGTGTTAACAAGTGGTTTGCTTAACATTGCACGTAA
AAAAGGGGAGGTGGAAATTTATATCCACAACTTGCACGATTATGGTTTAGGCCGTTACAAGCAAGTTGATGATGCGCCTT
ACGGCGGTGGAGCAGGTATGGTAATTCGTCCTGAGCCGGTGTTTGCTTGCATAGAGGCGTTACAAGCTGAGCGTCATTAC
GATGCTGTAATTTTTCTTACACCCGATGGAGAGTTGCTGGAGCAACCCTTAGCAAATCGGTTATCACGCTTAGAAAATCT
GCTGCTCCTGTGTGGGCATTACAAAGCAATTGATGAACGTATTCGTGAGCAACTCATTACTATGGAAATTTCCGTAGGTG
ATGTGGTGCTTTCAGGGGGAGAAATTCCTGCCTTAATGCTTATGGATGCCATTGTGCGCTTAATTCCCGGTGTGCTTGGC
GATAGTGAATCAGCATTAACCGACTCCTTTCAAAATGGATTGCTTGATGCTGCCTATTACACCCGTCCTGCTGATTTTAG
AGGTATGAAAGTTCCTGAAGTACTCTTAAGTGGACATCAGGCACATATTGAACAATGGCGCACCGCAAGCGCTCTTGAAC
GAACAAAAATTCGTCGTCCCGATTTGCTTGAGCGGGCTTGGCGAGAAGAGTTTTAG

Upstream 100 bases:

>100_bases
GCATTTGTAGAATCTTTTGAACTTGCAAGTAAAACCATTGTTATGTCCCGTTGTTGGGAATTTCTTTAAAAAAGAGAAAG
CTACTTGAGCTAAGTTAGTT

Downstream 100 bases:

>100_bases
ATAACATAAGAAGAAAAGGTTGTTGTATGAATCAGTTAATTCAGTTAGTTGAGTCATCTCTTGGTGCGAATCGCTTTGAA
CAAGTTCGTCCAGGTGACAC

Product: tRNA (guanine-N(1)-)-methyltransferase

Products: NA

Alternate protein names: M1G-methyltransferase; tRNA [GM37] methyltransferase

Number of amino acids: Translated: 231; Mature: 231

Protein sequence:

>231_residues
MRFDVISVIPYFFDSVLTSGLLNIARKKGEVEIYIHNLHDYGLGRYKQVDDAPYGGGAGMVIRPEPVFACIEALQAERHY
DAVIFLTPDGELLEQPLANRLSRLENLLLLCGHYKAIDERIREQLITMEISVGDVVLSGGEIPALMLMDAIVRLIPGVLG
DSESALTDSFQNGLLDAAYYTRPADFRGMKVPEVLLSGHQAHIEQWRTASALERTKIRRPDLLERAWREEF

Sequences:

>Translated_231_residues
MRFDVISVIPYFFDSVLTSGLLNIARKKGEVEIYIHNLHDYGLGRYKQVDDAPYGGGAGMVIRPEPVFACIEALQAERHY
DAVIFLTPDGELLEQPLANRLSRLENLLLLCGHYKAIDERIREQLITMEISVGDVVLSGGEIPALMLMDAIVRLIPGVLG
DSESALTDSFQNGLLDAAYYTRPADFRGMKVPEVLLSGHQAHIEQWRTASALERTKIRRPDLLERAWREEF
>Mature_231_residues
MRFDVISVIPYFFDSVLTSGLLNIARKKGEVEIYIHNLHDYGLGRYKQVDDAPYGGGAGMVIRPEPVFACIEALQAERHY
DAVIFLTPDGELLEQPLANRLSRLENLLLLCGHYKAIDERIREQLITMEISVGDVVLSGGEIPALMLMDAIVRLIPGVLG
DSESALTDSFQNGLLDAAYYTRPADFRGMKVPEVLLSGHQAHIEQWRTASALERTKIRRPDLLERAWREEF

Specific function: Specifically methylates guanosine-37 in various tRNAs

COG id: COG0336

COG function: function code J; tRNA-(guanine-N1)-methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase trmD family

Homologues:

Organism=Escherichia coli, GI1788959, Length=225, Percent_Identity=44.4444444444444, Blast_Score=182, Evalue=2e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMD_CHLCH (Q3ASF7)

Other databases:

- EMBL:   CP000108
- RefSeq:   YP_379111.1
- ProteinModelPortal:   Q3ASF7
- SMR:   Q3ASF7
- STRING:   Q3ASF7
- GeneID:   3747456
- GenomeReviews:   CP000108_GR
- KEGG:   cch:Cag_0802
- eggNOG:   COG0336
- HOGENOM:   HBG285805
- OMA:   VCGRFEG
- ProtClustDB:   PRK00026
- BioCyc:   CCHL340177:CAG_0802-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00605
- InterPro:   IPR016009
- InterPro:   IPR002649
- InterPro:   IPR023148
- Gene3D:   G3DSA:1.10.1270.20
- PIRSF:   PIRSF000386
- TIGRFAMs:   TIGR00088

Pfam domain/function: PF01746 tRNA_m1G_MT

EC number: =2.1.1.31

Molecular weight: Translated: 25997; Mature: 25997

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: NA

Important sites: BINDING 112-112

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRFDVISVIPYFFDSVLTSGLLNIARKKGEVEIYIHNLHDYGLGRYKQVDDAPYGGGAGM
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCE
VIRPEPVFACIEALQAERHYDAVIFLTPDGELLEQPLANRLSRLENLLLLCGHYKAIDER
EECCCHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IREQLITMEISVGDVVLSGGEIPALMLMDAIVRLIPGVLGDSESALTDSFQNGLLDAAYY
HHHHHHHHEEECCCEEECCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHH
TRPADFRGMKVPEVLLSGHQAHIEQWRTASALERTKIRRPDLLERAWREEF
CCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCC
>Mature Secondary Structure
MRFDVISVIPYFFDSVLTSGLLNIARKKGEVEIYIHNLHDYGLGRYKQVDDAPYGGGAGM
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCE
VIRPEPVFACIEALQAERHYDAVIFLTPDGELLEQPLANRLSRLENLLLLCGHYKAIDER
EECCCHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IREQLITMEISVGDVVLSGGEIPALMLMDAIVRLIPGVLGDSESALTDSFQNGLLDAAYY
HHHHHHHHEEECCCEEECCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHH
TRPADFRGMKVPEVLLSGHQAHIEQWRTASALERTKIRRPDLLERAWREEF
CCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA