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The map label for this gene is 78188087

Identifier: 78188087

GI number: 78188087

Start: 118404

End: 119819

Strand: Reverse

Name: 78188087

Synonym: Cag_0104

Alternate gene names: NA

Gene position: 119819-118404 (Counterclockwise)

Preceding gene: 78188088

Following gene: 78188086

Centisome position: 4.66

GC content: 48.66

Gene sequence:

>1416_bases
ATGGCAGAACATGTAAAAAAAGTGGCGTTAGTTTTTCTCCCCTCAGAAAGCGGTGTTGACGGCGCTCGTTCACTTTATGC
AAACAAAGCGGCACAGCACCCGCTTAAAGAGTGGGCAAACAACGCGTTTCGTGGCATTATTAAACGAAGCCAATTTGCCA
TTCCCCCGCTTTCGTTGATGATTCTTAGCTCACTTGAAGTAGCAGGTGTCCAGCAAGTGATCTGCGATCTCCGCTTTGAG
GATTTTGATTTTGAAATGAAGTGGGATTTAGTGGGCATTAGCGTCCAAAGTGGCATGGCTCGCAAAGCCTTTGAGCTTGC
TGATGCGCTTCGTGCGAAAGGAATAAAAGTAGCGCTTGGCGGTGCCCATGTAACCCTTTTCCCTGAAAGCTGCCAACCTC
ATGCGGATGTTTTGGTGCCCGGTGAAGCCGATGAAGTGTGGGAAGAGGCGTTGCGCGATCTTGTAGCGAATAGGCTCCAA
CCACTTTACCGTGCCGAAAGCTTCCCAAACCTTCAGCACGCCCGTCCCGTTAGCAAACAAGCGTTACAACCCGAACGCTA
CTTTACCACCAATTTGATACAAACAGGACGAGGCTGCCCATACAACTGCGACTTTTGCAATGTTCACGTCTTGAATGGGC
ACACCTTGCGCCAACGCCGTATTACCGATGTAGTGCAAGAAGTTGCTCGCTTTCAACAAGATGATCAGCGCATTTTCTTT
TTTGTTGACGACTCCATCAATGCTGATCCCGCTTACGCCTTAGAGCTTTTTCAATCCCTGACTCCCCTTAAAATCCGTTG
GTTTGGGCAAGCCACTACCACCTTAGGGCAGCAGCACGAACTCCTTAGCGCCTTTGCCGACTCAGGCTGCCAAGCATTAT
TGGTTGGCATTGAAAGTATTGAGAACGCCAGCCGCACAGCCCACGCTAAACAGCAAAACCGTGCAAACGAGTTAGTGAGC
GCCATAACCACCATTCGCCAAGCAGGCATTAGCCTTTACGGCAGCTTTATTTATGGACTTGATGGCGACACCCTCGAAAC
ACCCGCTGCAATTTTAGATTTTGTAGCACAAACAAAACTTGATGTACCCGGCATTAACATTTTACGCCCAACCCCAGGCA
CCCGCGTTTTTGAACGCCTCCGCAACGAAGGACGCTTACTGTTTGACCCAAATGATGTAACAGCATACCGCTACTCTTTT
GGACAAGAAATGCTCTATCGCCCAAAAAACATTCCACTTGACGACTTTATTGAAAGCTATAGCCAACTAACACGCACTCT
TTTTTCATGGCAAAACGCCGTTAAACGAGGATTAAACGCCCCACGAGCAAAAAGCGCCGTCCTGCTTTTTAACCTCTTCT
ATAGCCACCTTTACACCCTCTCGCGCAACGACCTGCAAGCACAAAAACTATCGTAA

Upstream 100 bases:

>100_bases
GCAAGCCGCTCGCAATGAATCATCGCTGATGCGCGAAATGGCACAACAAGGCAAAAGCAGCACCCCACGTTCTACCAAAA
ACTTGCGTGATTGGTAAGCT

Downstream 100 bases:

>100_bases
TACAATAAAACGGAGATTCCTGCTTACACAATAAGGTTATGGCGTTCCCGTAACCGCACGAAAAACCATTTTGCCATCCG
CTGAATAATGTTTAAAATGG

Product: Elongator protein 3/MiaB/NifB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 471; Mature: 470

Protein sequence:

>471_residues
MAEHVKKVALVFLPSESGVDGARSLYANKAAQHPLKEWANNAFRGIIKRSQFAIPPLSLMILSSLEVAGVQQVICDLRFE
DFDFEMKWDLVGISVQSGMARKAFELADALRAKGIKVALGGAHVTLFPESCQPHADVLVPGEADEVWEEALRDLVANRLQ
PLYRAESFPNLQHARPVSKQALQPERYFTTNLIQTGRGCPYNCDFCNVHVLNGHTLRQRRITDVVQEVARFQQDDQRIFF
FVDDSINADPAYALELFQSLTPLKIRWFGQATTTLGQQHELLSAFADSGCQALLVGIESIENASRTAHAKQQNRANELVS
AITTIRQAGISLYGSFIYGLDGDTLETPAAILDFVAQTKLDVPGINILRPTPGTRVFERLRNEGRLLFDPNDVTAYRYSF
GQEMLYRPKNIPLDDFIESYSQLTRTLFSWQNAVKRGLNAPRAKSAVLLFNLFYSHLYTLSRNDLQAQKLS

Sequences:

>Translated_471_residues
MAEHVKKVALVFLPSESGVDGARSLYANKAAQHPLKEWANNAFRGIIKRSQFAIPPLSLMILSSLEVAGVQQVICDLRFE
DFDFEMKWDLVGISVQSGMARKAFELADALRAKGIKVALGGAHVTLFPESCQPHADVLVPGEADEVWEEALRDLVANRLQ
PLYRAESFPNLQHARPVSKQALQPERYFTTNLIQTGRGCPYNCDFCNVHVLNGHTLRQRRITDVVQEVARFQQDDQRIFF
FVDDSINADPAYALELFQSLTPLKIRWFGQATTTLGQQHELLSAFADSGCQALLVGIESIENASRTAHAKQQNRANELVS
AITTIRQAGISLYGSFIYGLDGDTLETPAAILDFVAQTKLDVPGINILRPTPGTRVFERLRNEGRLLFDPNDVTAYRYSF
GQEMLYRPKNIPLDDFIESYSQLTRTLFSWQNAVKRGLNAPRAKSAVLLFNLFYSHLYTLSRNDLQAQKLS
>Mature_470_residues
AEHVKKVALVFLPSESGVDGARSLYANKAAQHPLKEWANNAFRGIIKRSQFAIPPLSLMILSSLEVAGVQQVICDLRFED
FDFEMKWDLVGISVQSGMARKAFELADALRAKGIKVALGGAHVTLFPESCQPHADVLVPGEADEVWEEALRDLVANRLQP
LYRAESFPNLQHARPVSKQALQPERYFTTNLIQTGRGCPYNCDFCNVHVLNGHTLRQRRITDVVQEVARFQQDDQRIFFF
VDDSINADPAYALELFQSLTPLKIRWFGQATTTLGQQHELLSAFADSGCQALLVGIESIENASRTAHAKQQNRANELVSA
ITTIRQAGISLYGSFIYGLDGDTLETPAAILDFVAQTKLDVPGINILRPTPGTRVFERLRNEGRLLFDPNDVTAYRYSFG
QEMLYRPKNIPLDDFIESYSQLTRTLFSWQNAVKRGLNAPRAKSAVLLFNLFYSHLYTLSRNDLQAQKLS

Specific function: Unknown

COG id: COG1032

COG function: function code C; Fe-S oxidoreductase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 B12-binding domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006158
- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR007197 [H]

Pfam domain/function: PF02310 B12-binding; PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 52839; Mature: 52708

Theoretical pI: Translated: 7.05; Mature: 7.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEHVKKVALVFLPSESGVDGARSLYANKAAQHPLKEWANNAFRGIIKRSQFAIPPLSLM
CCHHHEEEEEEEECCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCHHHH
ILSSLEVAGVQQVICDLRFEDFDFEMKWDLVGISVQSGMARKAFELADALRAKGIKVALG
HHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEEEHHCCHHHHHHHHHHHHHHCCEEEEEC
GAHVTLFPESCQPHADVLVPGEADEVWEEALRDLVANRLQPLYRAESFPNLQHARPVSKQ
CEEEEEECCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCHHH
ALQPERYFTTNLIQTGRGCPYNCDFCNVHVLNGHTLRQRRITDVVQEVARFQQDDQRIFF
HCCCHHHHHHHHHHCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHCCCCCEEEE
FVDDSINADPAYALELFQSLTPLKIRWFGQATTTLGQQHELLSAFADSGCQALLVGIESI
EECCCCCCCHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHHCCCHHEEHHHHHHH
ENASRTAHAKQQNRANELVSAITTIRQAGISLYGSFIYGLDGDTLETPAAILDFVAQTKL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCC
DVPGINILRPTPGTRVFERLRNEGRLLFDPNDVTAYRYSFGQEMLYRPKNIPLDDFIESY
CCCCCEEECCCCCHHHHHHHHHCCCEEECCCCCEEHHHHHCHHHHCCCCCCCHHHHHHHH
SQLTRTLFSWQNAVKRGLNAPRAKSAVLLFNLFYSHLYTLSRNDLQAQKLS
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHCCC
>Mature Secondary Structure 
AEHVKKVALVFLPSESGVDGARSLYANKAAQHPLKEWANNAFRGIIKRSQFAIPPLSLM
CHHHEEEEEEEECCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCHHHH
ILSSLEVAGVQQVICDLRFEDFDFEMKWDLVGISVQSGMARKAFELADALRAKGIKVALG
HHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEEEHHCCHHHHHHHHHHHHHHCCEEEEEC
GAHVTLFPESCQPHADVLVPGEADEVWEEALRDLVANRLQPLYRAESFPNLQHARPVSKQ
CEEEEEECCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCHHH
ALQPERYFTTNLIQTGRGCPYNCDFCNVHVLNGHTLRQRRITDVVQEVARFQQDDQRIFF
HCCCHHHHHHHHHHCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHCCCCCEEEE
FVDDSINADPAYALELFQSLTPLKIRWFGQATTTLGQQHELLSAFADSGCQALLVGIESI
EECCCCCCCHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHHCCCHHEEHHHHHHH
ENASRTAHAKQQNRANELVSAITTIRQAGISLYGSFIYGLDGDTLETPAAILDFVAQTKL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCC
DVPGINILRPTPGTRVFERLRNEGRLLFDPNDVTAYRYSFGQEMLYRPKNIPLDDFIESY
CCCCCEEECCCCCHHHHHHHHHCCCEEECCCCCEEHHHHHCHHHHCCCCCCCHHHHHHHH
SQLTRTLFSWQNAVKRGLNAPRAKSAVLLFNLFYSHLYTLSRNDLQAQKLS
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]