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The map label for this gene is htrB [H]

Identifier: 78067901

GI number: 78067901

Start: 3599425

End: 3600309

Strand: Direct

Name: htrB [H]

Synonym: Bcep18194_A6432

Alternate gene names: 78067901

Gene position: 3599425-3600309 (Clockwise)

Preceding gene: 78067899

Following gene: 161702953

Centisome position: 97.44

GC content: 64.07

Gene sequence:

>885_bases
ATGCTAGGTCGTCTAGGCACGCACCTCGCCATTGGCTTGCTGAAACTGCTCGCCCTGTTGCCGTACGGCCTGACCGCACG
GTTCGGCGATGGTCTCGGCTGGCTGCTCTACAAGATCCCCAGCCGGCGAAAACGCATCGTACACACCAATCTGAAGCTCT
GCTTCCCGGATTGGAGCGACGAGCGTCGCGAGGAAGTCGCCGGGCAGCATTTCCGCCATGCGATCCGCAGCTACGTCGAG
CGCAGCTACCAGTGGTTCGCGTCGGAACAGGCCTACCGCAAGCTGTTTACGGTCGAGAGCGAAGTCGATCTCAGCGATCC
GGACATGCCCCCGACCCTCCTGCTCGGCTTCCATTTCGTCGGCATCGAGGCCGGCTCGATGGCGATCAATCTCGCCCTCA
ACCGGCCATGCGGCTCGCTCTACACGCCGATGAAGAGCCCGGAAATCGAAGCCGCGGCCAAAGCCGGCCGCAGCCGCTTC
GGCGCTGAACTGGCGAGCCGTGCCGACAGCGCGCGTACCGTGCTGCGCTGGCTGCGCGACCGCAAGCCGGTGATGCTGGG
TGCAGACATGGATTACGGGCTGCGCAACTCGACGTTCGTACCCTTCTTCGGCATACCTGCCTGCACGCTGACGGCGGTCG
GCCGGTTCGCAAAGACCGGCCACGCGCAGGTTCTGCCCTTCATCGGCGAGGTGCTGCCGAACTACAAGGGCTACCGTCTG
AAGGTCTTCAAGCCGTGGGCCGACTACCCGACCGGCGACGACGATCTCGACGCGCGGCGGATGAACGAATTCCTCGAGGA
ACAGATCCCGCTGATGCCCGAGCAATACTATTGGGTCCACAAGCGCTTCAAGACGCGCCCGGCCGGTGAGCCGAGCCTGT
ACTGA

Upstream 100 bases:

>100_bases
CTTTCCTGAATTGTCACAGAGCGCCGCCCGTGCTGCATCTTCCGCTGTTTCACCATCCTGTTTGCAAAGCGCCGGCCAGC
CGGTCCCACGGAGGTTTCGC

Downstream 100 bases:

>100_bases
GCCATGCTCGCGGGCGCGCGGTCACGTGCGTGCCCGCGCTCTCGCTACCTTGGGCACGCCTTTCGCGCGTGCCTGCCACA
AGTCGTAAGCGGATTGCTGC

Product: lipid A biosynthesis lauroyl acyltransferase

Products: KDO2-(lauroyl)-lipid IVA [C]

Alternate protein names: Heat shock protein B [H]

Number of amino acids: Translated: 294; Mature: 294

Protein sequence:

>294_residues
MLGRLGTHLAIGLLKLLALLPYGLTARFGDGLGWLLYKIPSRRKRIVHTNLKLCFPDWSDERREEVAGQHFRHAIRSYVE
RSYQWFASEQAYRKLFTVESEVDLSDPDMPPTLLLGFHFVGIEAGSMAINLALNRPCGSLYTPMKSPEIEAAAKAGRSRF
GAELASRADSARTVLRWLRDRKPVMLGADMDYGLRNSTFVPFFGIPACTLTAVGRFAKTGHAQVLPFIGEVLPNYKGYRL
KVFKPWADYPTGDDDLDARRMNEFLEEQIPLMPEQYYWVHKRFKTRPAGEPSLY

Sequences:

>Translated_294_residues
MLGRLGTHLAIGLLKLLALLPYGLTARFGDGLGWLLYKIPSRRKRIVHTNLKLCFPDWSDERREEVAGQHFRHAIRSYVE
RSYQWFASEQAYRKLFTVESEVDLSDPDMPPTLLLGFHFVGIEAGSMAINLALNRPCGSLYTPMKSPEIEAAAKAGRSRF
GAELASRADSARTVLRWLRDRKPVMLGADMDYGLRNSTFVPFFGIPACTLTAVGRFAKTGHAQVLPFIGEVLPNYKGYRL
KVFKPWADYPTGDDDLDARRMNEFLEEQIPLMPEQYYWVHKRFKTRPAGEPSLY
>Mature_294_residues
MLGRLGTHLAIGLLKLLALLPYGLTARFGDGLGWLLYKIPSRRKRIVHTNLKLCFPDWSDERREEVAGQHFRHAIRSYVE
RSYQWFASEQAYRKLFTVESEVDLSDPDMPPTLLLGFHFVGIEAGSMAINLALNRPCGSLYTPMKSPEIEAAAKAGRSRF
GAELASRADSARTVLRWLRDRKPVMLGADMDYGLRNSTFVPFFGIPACTLTAVGRFAKTGHAQVLPFIGEVLPNYKGYRL
KVFKPWADYPTGDDDLDARRMNEFLEEQIPLMPEQYYWVHKRFKTRPAGEPSLY

Specific function: Acylates the intermediate (KDO)2-lipid IVA to form (KDO)2-(lauroyl)-lipid IVA. Has 10 fold selectivity for lauroyl- ACP over myristoyl-ACP [H]

COG id: COG1560

COG function: function code M; Lauroyl/myristoyl acyltransferase

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the htrB/msbB family [H]

Homologues:

Organism=Escherichia coli, GI1787292, Length=291, Percent_Identity=34.3642611683849, Blast_Score=162, Evalue=4e-41,
Organism=Escherichia coli, GI87082094, Length=291, Percent_Identity=32.6460481099656, Blast_Score=151, Evalue=5e-38,
Organism=Escherichia coli, GI1788161, Length=301, Percent_Identity=27.2425249169435, Blast_Score=90, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004960
- InterPro:   IPR011920 [H]

Pfam domain/function: PF03279 Lip_A_acyltrans [H]

EC number: 2.3.1.- [C]

Molecular weight: Translated: 33477; Mature: 33477

Theoretical pI: Translated: 9.64; Mature: 9.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLGRLGTHLAIGLLKLLALLPYGLTARFGDGLGWLLYKIPSRRKRIVHTNLKLCFPDWSD
CCCCHHHHHHHHHHHHHHHCCCCCHHHHCCCHHHHHHHCCCHHHHEEECCCEEECCCCCH
ERREEVAGQHFRHAIRSYVERSYQWFASEQAYRKLFTVESEVDLSDPDMPPTLLLGFHFV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHE
GIEAGSMAINLALNRPCGSLYTPMKSPEIEAAAKAGRSRFGAELASRADSARTVLRWLRD
EEECCCEEEEEEECCCCCHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
RKPVMLGADMDYGLRNSTFVPFFGIPACTLTAVGRFAKTGHAQVLPFIGEVLPNYKGYRL
CCCEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCEE
KVFKPWADYPTGDDDLDARRMNEFLEEQIPLMPEQYYWVHKRFKTRPAGEPSLY
EEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MLGRLGTHLAIGLLKLLALLPYGLTARFGDGLGWLLYKIPSRRKRIVHTNLKLCFPDWSD
CCCCHHHHHHHHHHHHHHHCCCCCHHHHCCCHHHHHHHCCCHHHHEEECCCEEECCCCCH
ERREEVAGQHFRHAIRSYVERSYQWFASEQAYRKLFTVESEVDLSDPDMPPTLLLGFHFV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHE
GIEAGSMAINLALNRPCGSLYTPMKSPEIEAAAKAGRSRFGAELASRADSARTVLRWLRD
EEECCCEEEEEEECCCCCHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
RKPVMLGADMDYGLRNSTFVPFFGIPACTLTAVGRFAKTGHAQVLPFIGEVLPNYKGYRL
CCCEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCEE
KVFKPWADYPTGDDDLDARRMNEFLEEQIPLMPEQYYWVHKRFKTRPAGEPSLY
EEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: KDO2-lipid IVA; lauroyl-ACP [C]

Specific reaction: KDO2-lipid IVA + lauroyl-ACP = KDO2-(lauroyl)-lipid IVA + [acyl carrier protein] [C]

General reaction: Acyltransferases [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]