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The map label for this gene is alkJ [H]

Identifier: 78067888

GI number: 78067888

Start: 3587319

End: 3588959

Strand: Reverse

Name: alkJ [H]

Synonym: Bcep18194_A6419

Alternate gene names: 78067888

Gene position: 3588959-3587319 (Counterclockwise)

Preceding gene: 78067889

Following gene: 78067881

Centisome position: 97.15

GC content: 69.04

Gene sequence:

>1641_bases
ATGCAATACGACTACATCATCGTCGGCGGCGGTTCGGGCGGGTCGAGCCTGGCAGGCCGTCTCGCCGATGCCTGCCCCGA
TGCGACGATCGCGCTGATCGAAGCCGGTTCGCATACGGAGCGCAACCTGCTCGTCAACATGCCGGTCGGCATCGCGGCGC
TGGTGCCGTTCAAGCTCGGTACGAACTACGGCTACGAGACGGTGCCGCAGCCGGGCCTCGGCGGGCGGCGCGGCTACCAG
CCGCGCGGGCGCGGGATGGGCGGCTCGAGCGCGATCAACGCGATGATCTACACGCGCGGCCACCCGGGAGACTACGACGA
ATGGGCGCAGCTCGGCGCGACCGGCTGGGGCTGGCAGGACGTGCTGCCGTATTTCCGCCGGGCGGAAGGCAACGCGCGCG
GTGCCGATGCGTGGCACGGTGCAGACGGCCCGCTCACGGTGTCGGATCTGCGCTTTCGCAATCCGTTCTCCGAACGATTC
ATCCAGGCCGCGCATGCGGCCGGCTATCCGCTGAACGACGACTTCAACGGCGCGACGCAGGAAGGCGTCGGTTTCTATCA
GGTGACGCATCGCGACGGCTCGCGCTGCAGCGTCGCCCGCGCGTACATCTACGGCCGCAACCGACCGAACCTGCACGTGA
TCACCGATGCGACGGTGCTGCGGGTCAGGTTCGACGGCAAGCGCGCGGCCGGCGTCGCGGTTGCGCGCAACGGGCGCGTC
GAGACGCTCGACGCGCGCGCGGAAGTGATCCTGTCGGCCGGCGCGTTCAATTCGCCGCAACTGCTGATGTGTTCGGGGAT
CGGGCCGGCGGAGCAGCTGCGCCGGCACGGGATCGCGGTCGTGCAGGATGCGCCGGACGTCGGCACGAACCTGATCGACC
ATATCGATTTCATCATCAATACGCGCGTGAATTCATCGGAACTGGTCGGTGTGTGCCTGCGCGGGATCGCGAAGATGACG
CCCGCGCTCGCGCGCTATTTCTCGAGCCGCACCGGGATGATGACGAGCAACGTCGCGGAGGCCGGCGGCTTCATCAAGAG
CGATCCGTCGCTCGAGCGTCCCGACCTGCAACTGCATTTCTGCACGGCGCTCGTGGACGACCACAACCGCAAGATGCACT
GGGGCTTCGGCTATTCGCTGCACGTGTGCGCGCTGCGGCCGTTCAGCCGCGGCACGGTGGCGCTCGCGAGCGGCGATGCG
CGCGACGCACCGCTGATCGATCCGCGCTTCTTCAGCGATTCGCGGGATCTCGACCTGCTCGTGCGCGGCACGCAGGCGAT
GCGCACGATCCTGTCGCAGGCGCCGCTCGCGTCGCAGGGCGGGCGCGAGCTGTACACGCGGGCCGACCAGAGCGAAGCGG
AGCTGCGCAAGACGATCGTCGAGCACGCCGACACGATCTACCACCCGGTCGGCACCTGCCGGATGGGTTCGGATGCGCGC
GCGGTCGTCGATCCGCAACTGCGCGTGCGCGGCGTCGAAGGGTTGCGCGTGGTGGATGCGTCGGTGATGCCGACGTTGGT
CGGCGGGAACACGAATGCGCCGTCGGTGATGATCGGCGAACGCGCGGCGGACTTCATCGTCGCCACGCGCAAGGGCGCGG
CGCCGCGCGGCGAGGCAGCCGCTGCGGTGCACGGCCGCTGA

Upstream 100 bases:

>100_bases
CCGCGCGTGCGACGGCCATTCCTACAATGCCCGAATTACAAGAGGGGACGCGGTCGCGCGGCGGTGCCGGGCGCCTTCCA
TGACACGACGGAGACGTGAC

Downstream 100 bases:

>100_bases
GCGGCGCGCGCACGGCGGCGGCCATGCTCAGGCAAACGTATCGACGAGGCCGGCGCGGCTCACGCTCGCGGCGGCCGGTG
CGGATTGCGCAGCGCCGACC

Product: glucose-methanol-choline oxidoreductase

Products: Betaine Aldehyde; Reduced Acceptor. [C]

Alternate protein names: NA

Number of amino acids: Translated: 546; Mature: 546

Protein sequence:

>546_residues
MQYDYIIVGGGSGGSSLAGRLADACPDATIALIEAGSHTERNLLVNMPVGIAALVPFKLGTNYGYETVPQPGLGGRRGYQ
PRGRGMGGSSAINAMIYTRGHPGDYDEWAQLGATGWGWQDVLPYFRRAEGNARGADAWHGADGPLTVSDLRFRNPFSERF
IQAAHAAGYPLNDDFNGATQEGVGFYQVTHRDGSRCSVARAYIYGRNRPNLHVITDATVLRVRFDGKRAAGVAVARNGRV
ETLDARAEVILSAGAFNSPQLLMCSGIGPAEQLRRHGIAVVQDAPDVGTNLIDHIDFIINTRVNSSELVGVCLRGIAKMT
PALARYFSSRTGMMTSNVAEAGGFIKSDPSLERPDLQLHFCTALVDDHNRKMHWGFGYSLHVCALRPFSRGTVALASGDA
RDAPLIDPRFFSDSRDLDLLVRGTQAMRTILSQAPLASQGGRELYTRADQSEAELRKTIVEHADTIYHPVGTCRMGSDAR
AVVDPQLRVRGVEGLRVVDASVMPTLVGGNTNAPSVMIGERAADFIVATRKGAAPRGEAAAAVHGR

Sequences:

>Translated_546_residues
MQYDYIIVGGGSGGSSLAGRLADACPDATIALIEAGSHTERNLLVNMPVGIAALVPFKLGTNYGYETVPQPGLGGRRGYQ
PRGRGMGGSSAINAMIYTRGHPGDYDEWAQLGATGWGWQDVLPYFRRAEGNARGADAWHGADGPLTVSDLRFRNPFSERF
IQAAHAAGYPLNDDFNGATQEGVGFYQVTHRDGSRCSVARAYIYGRNRPNLHVITDATVLRVRFDGKRAAGVAVARNGRV
ETLDARAEVILSAGAFNSPQLLMCSGIGPAEQLRRHGIAVVQDAPDVGTNLIDHIDFIINTRVNSSELVGVCLRGIAKMT
PALARYFSSRTGMMTSNVAEAGGFIKSDPSLERPDLQLHFCTALVDDHNRKMHWGFGYSLHVCALRPFSRGTVALASGDA
RDAPLIDPRFFSDSRDLDLLVRGTQAMRTILSQAPLASQGGRELYTRADQSEAELRKTIVEHADTIYHPVGTCRMGSDAR
AVVDPQLRVRGVEGLRVVDASVMPTLVGGNTNAPSVMIGERAADFIVATRKGAAPRGEAAAAVHGR
>Mature_546_residues
MQYDYIIVGGGSGGSSLAGRLADACPDATIALIEAGSHTERNLLVNMPVGIAALVPFKLGTNYGYETVPQPGLGGRRGYQ
PRGRGMGGSSAINAMIYTRGHPGDYDEWAQLGATGWGWQDVLPYFRRAEGNARGADAWHGADGPLTVSDLRFRNPFSERF
IQAAHAAGYPLNDDFNGATQEGVGFYQVTHRDGSRCSVARAYIYGRNRPNLHVITDATVLRVRFDGKRAAGVAVARNGRV
ETLDARAEVILSAGAFNSPQLLMCSGIGPAEQLRRHGIAVVQDAPDVGTNLIDHIDFIINTRVNSSELVGVCLRGIAKMT
PALARYFSSRTGMMTSNVAEAGGFIKSDPSLERPDLQLHFCTALVDDHNRKMHWGFGYSLHVCALRPFSRGTVALASGDA
RDAPLIDPRFFSDSRDLDLLVRGTQAMRTILSQAPLASQGGRELYTRADQSEAELRKTIVEHADTIYHPVGTCRMGSDAR
AVVDPQLRVRGVEGLRVVDASVMPTLVGGNTNAPSVMIGERAADFIVATRKGAAPRGEAAAAVHGR

Specific function: Converts aliphatic medium-chain-length alcohols into aldehydes. May be linked to the electron transfer chain [H]

COG id: COG2303

COG function: function code E; Choline dehydrogenase and related flavoproteins

Gene ontology:

Cell location: Cell inner membrane [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GMC oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI217272839, Length=535, Percent_Identity=42.2429906542056, Blast_Score=388, Evalue=1e-108,
Organism=Escherichia coli, GI1786503, Length=554, Percent_Identity=38.9891696750903, Blast_Score=339, Evalue=3e-94,
Organism=Caenorhabditis elegans, GI17532301, Length=558, Percent_Identity=37.6344086021505, Blast_Score=348, Evalue=4e-96,
Organism=Drosophila melanogaster, GI45549471, Length=572, Percent_Identity=38.8111888111888, Blast_Score=330, Evalue=2e-90,
Organism=Drosophila melanogaster, GI45551458, Length=572, Percent_Identity=38.8111888111888, Blast_Score=330, Evalue=2e-90,
Organism=Drosophila melanogaster, GI24642048, Length=582, Percent_Identity=37.2852233676976, Blast_Score=314, Evalue=1e-85,
Organism=Drosophila melanogaster, GI24642042, Length=573, Percent_Identity=37.17277486911, Blast_Score=311, Evalue=1e-84,
Organism=Drosophila melanogaster, GI24642055, Length=581, Percent_Identity=35.1118760757315, Blast_Score=298, Evalue=7e-81,
Organism=Drosophila melanogaster, GI24642037, Length=579, Percent_Identity=36.6148531951641, Blast_Score=290, Evalue=1e-78,
Organism=Drosophila melanogaster, GI24642039, Length=571, Percent_Identity=35.7267950963222, Blast_Score=286, Evalue=2e-77,
Organism=Drosophila melanogaster, GI24642059, Length=577, Percent_Identity=34.315424610052, Blast_Score=286, Evalue=3e-77,
Organism=Drosophila melanogaster, GI24650267, Length=575, Percent_Identity=35.8260869565217, Blast_Score=285, Evalue=6e-77,
Organism=Drosophila melanogaster, GI24642035, Length=581, Percent_Identity=34.9397590361446, Blast_Score=279, Evalue=3e-75,
Organism=Drosophila melanogaster, GI18859995, Length=571, Percent_Identity=35.3765323992995, Blast_Score=275, Evalue=6e-74,
Organism=Drosophila melanogaster, GI17137792, Length=566, Percent_Identity=33.3922261484099, Blast_Score=268, Evalue=6e-72,
Organism=Drosophila melanogaster, GI18859993, Length=571, Percent_Identity=33.0998248686515, Blast_Score=266, Evalue=3e-71,
Organism=Drosophila melanogaster, GI24642051, Length=577, Percent_Identity=30.5025996533795, Blast_Score=243, Evalue=3e-64,
Organism=Drosophila melanogaster, GI24642057, Length=582, Percent_Identity=30.7560137457045, Blast_Score=161, Evalue=9e-40,
Organism=Drosophila melanogaster, GI24645930, Length=583, Percent_Identity=28.3018867924528, Blast_Score=150, Evalue=2e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012132
- InterPro:   IPR000172
- InterPro:   IPR007867 [H]

Pfam domain/function: PF05199 GMC_oxred_C; PF00732 GMC_oxred_N [H]

EC number: 1.1.99.1 [C]

Molecular weight: Translated: 58598; Mature: 58598

Theoretical pI: Translated: 8.29; Mature: 8.29

Prosite motif: PS00624 GMC_OXRED_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQYDYIIVGGGSGGSSLAGRLADACPDATIALIEAGSHTERNLLVNMPVGIAALVPFKLG
CCEEEEEEECCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCEEEEECCCCEEEEEEEEEC
TNYGYETVPQPGLGGRRGYQPRGRGMGGSSAINAMIYTRGHPGDYDEWAQLGATGWGWQD
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHEEEEEEECCCCCCHHHHHHHCCCCCCHHH
VLPYFRRAEGNARGADAWHGADGPLTVSDLRFRNPFSERFIQAAHAAGYPLNDDFNGATQ
HHHHHHHCCCCCCCCCCCCCCCCCEEHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCC
EGVGFYQVTHRDGSRCSVARAYIYGRNRPNLHVITDATVLRVRFDGKRAAGVAVARNGRV
CCCEEEEEECCCCCCEEEEEEEEEECCCCCEEEEECCEEEEEEECCCCCCCEEEECCCCE
ETLDARAEVILSAGAFNSPQLLMCSGIGPAEQLRRHGIAVVQDAPDVGTNLIDHIDFIIN
EEECCCEEEEEECCCCCCCCEEEECCCCHHHHHHHCCEEEEECCCCCCHHHHHHHHHEEE
TRVNSSELVGVCLRGIAKMTPALARYFSSRTGMMTSNVAEAGGFIKSDPSLERPDLQLHF
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCHHHHCCEECCCCCCCCCCCEEEE
CTALVDDHNRKMHWGFGYSLHVCALRPFSRGTVALASGDARDAPLIDPRFFSDSRDLDLL
EEEECCCCCCEEEEECCCEEEEEEECCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCEEE
VRGTQAMRTILSQAPLASQGGRELYTRADQSEAELRKTIVEHADTIYHPVGTCRMGSDAR
EECHHHHHHHHHCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCEECCCCCC
AVVDPQLRVRGVEGLRVVDASVMPTLVGGNTNAPSVMIGERAADFIVATRKGAAPRGEAA
EEECCCCEEECCCCEEEECCCCCCEEECCCCCCCEEEECCCCCCEEEEECCCCCCCCCCC
AAVHGR
EECCCC
>Mature Secondary Structure
MQYDYIIVGGGSGGSSLAGRLADACPDATIALIEAGSHTERNLLVNMPVGIAALVPFKLG
CCEEEEEEECCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCEEEEECCCCEEEEEEEEEC
TNYGYETVPQPGLGGRRGYQPRGRGMGGSSAINAMIYTRGHPGDYDEWAQLGATGWGWQD
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHEEEEEEECCCCCCHHHHHHHCCCCCCHHH
VLPYFRRAEGNARGADAWHGADGPLTVSDLRFRNPFSERFIQAAHAAGYPLNDDFNGATQ
HHHHHHHCCCCCCCCCCCCCCCCCEEHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCC
EGVGFYQVTHRDGSRCSVARAYIYGRNRPNLHVITDATVLRVRFDGKRAAGVAVARNGRV
CCCEEEEEECCCCCCEEEEEEEEEECCCCCEEEEECCEEEEEEECCCCCCCEEEECCCCE
ETLDARAEVILSAGAFNSPQLLMCSGIGPAEQLRRHGIAVVQDAPDVGTNLIDHIDFIIN
EEECCCEEEEEECCCCCCCCEEEECCCCHHHHHHHCCEEEEECCCCCCHHHHHHHHHEEE
TRVNSSELVGVCLRGIAKMTPALARYFSSRTGMMTSNVAEAGGFIKSDPSLERPDLQLHF
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCHHHHCCEECCCCCCCCCCCEEEE
CTALVDDHNRKMHWGFGYSLHVCALRPFSRGTVALASGDARDAPLIDPRFFSDSRDLDLL
EEEECCCCCCEEEEECCCEEEEEEECCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCEEE
VRGTQAMRTILSQAPLASQGGRELYTRADQSEAELRKTIVEHADTIYHPVGTCRMGSDAR
EECHHHHHHHHHCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCEECCCCCC
AVVDPQLRVRGVEGLRVVDASVMPTLVGGNTNAPSVMIGERAADFIVATRKGAAPRGEAA
EEECCCCEEECCCCEEEECCCCCCEEECCCCCCCEEEECCCCCCEEEEECCCCCCCCCCC
AAVHGR
EECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Coenzyme Q [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 7 {choline}} 1.1 {phenazine}} [C]

Substrates: Choline; Acceptor [C]

Specific reaction: Choline + Acceptor = Betaine Aldehyde + Reduced Acceptor. [C]

General reaction: Redox reaction [C]

Inhibitor: 2-Dimethylaminoethanol; Betaine aldehyde; Monoethanolamine; Semicarbazide [C]

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11390693 [H]