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The map label for this gene is sbp [H]

Identifier: 78067766

GI number: 78067766

Start: 3463081

End: 3464100

Strand: Reverse

Name: sbp [H]

Synonym: Bcep18194_A6297

Alternate gene names: 78067766

Gene position: 3464100-3463081 (Counterclockwise)

Preceding gene: 78067768

Following gene: 78067765

Centisome position: 93.77

GC content: 66.27

Gene sequence:

>1020_bases
ATGGCAAGCATGAAGGGGATCGGTCGCTGGCTGCACACGGGCGCGGCGGCGGCGCTGGTCGTGGCGGCGACGGCCGCGCA
CGCGGATACGTCGATCCTGAACGTGTCGTACGACGTGACGCGCGAACTGTACAAGGACATCAACGCGGGTTTTGCCGCTG
CCTACAAGCAGAAGACCGGCGAGACGATCGCGATCAAGCAGTCGCACGGCGCGTCGAGCGCGCAGGCGCTGTCGGTGCTG
CAAGGGCTGCAGGCCGACGTCGTGACGATGAACCAGCCGAACGACATCGACCTGCTCGCCGAGCGTGGCCAGTTGCTGCC
GAAGGACTGGCGCGCGCGCTTCCCGGACAACAGCTCGCCGTACTCGACCACGATGGTGTTCCTCGTGCGCAAGGGCAACC
CGAAGGCGATCAAGGACTGGAGCGATCTCGCGAAGCCGGGTGTCCAGGTGGTCATCGCGAACCCGAAGACGTCGGGCAAC
GGCCGCTATGCGTATCTCGCCGCGTGGGGCTTCCAGAAGCAGAAGGGCGCGACCGACCAGCAGGCGATCGATTTCGAGAA
GGCGATCTTCCGCAACGTGCCGGTGCTCGACTCCGGCGGCCGCGGCGCGACGACGACGTTCACGCAGCGCGGCATCGGCG
ACGTGCTGGTCACGTTCGAGAACGAAGGCGCGCTGATGGACACCGGCGCATCGGGCGCGCAGTTCGACGCGGTGTACCCG
TCGGCGAGCATCCTCGCGGAGCCGCCCGTCGCCGTCGTCGACAAGGTCGTCGACAAGAAGGGCACGCGCAAGGTCGCGCA
GGCGTATCTCGACTACCTGTACACGCCGGAAGCGCAGGAGATCATCGCGCAACATCATCTGCGCCCGCGCGACGCGAACG
TGCTTAAGAAGCATGCGGCCGAATTCAAGCCGCTGAAGACGTTCAGCGTCGAGCAGGTCTTCGGCAGCTGGGCGAACGCG
CAGAAGACGCACTTCGCGGACGGCGGCACGTTCGACCAGGTGATCGTCGACCGCAAGTGA

Upstream 100 bases:

>100_bases
CGCCGCGCACGGCCGCTAGAATGCCCGCTTACGTTTCGATGACAGCGCGTACGCGTGCCGTCACGCACATTCACCACCAC
TGGAAACGGGGCATATCACG

Downstream 100 bases:

>100_bases
GCGCCGCGCGCACGGCGCGCTGGTCCTCACGCTGACGACGCAGCCGGCCGCTTCCGCGGCCGGTTTTTTTTGCGCCGCGC
GGCCGGCGGTGCGCAGGATT

Product: ABC thiosulphate transporter, periplasmic ligand binding protein

Products: ADP; phosphate; S2O32- [Cytoplasm]; SO42- [Cytoplasm] [C]

Alternate protein names: Sulfate starvation-induced protein 2; SSI2 [H]

Number of amino acids: Translated: 339; Mature: 338

Protein sequence:

>339_residues
MASMKGIGRWLHTGAAAALVVAATAAHADTSILNVSYDVTRELYKDINAGFAAAYKQKTGETIAIKQSHGASSAQALSVL
QGLQADVVTMNQPNDIDLLAERGQLLPKDWRARFPDNSSPYSTTMVFLVRKGNPKAIKDWSDLAKPGVQVVIANPKTSGN
GRYAYLAAWGFQKQKGATDQQAIDFEKAIFRNVPVLDSGGRGATTTFTQRGIGDVLVTFENEGALMDTGASGAQFDAVYP
SASILAEPPVAVVDKVVDKKGTRKVAQAYLDYLYTPEAQEIIAQHHLRPRDANVLKKHAAEFKPLKTFSVEQVFGSWANA
QKTHFADGGTFDQVIVDRK

Sequences:

>Translated_339_residues
MASMKGIGRWLHTGAAAALVVAATAAHADTSILNVSYDVTRELYKDINAGFAAAYKQKTGETIAIKQSHGASSAQALSVL
QGLQADVVTMNQPNDIDLLAERGQLLPKDWRARFPDNSSPYSTTMVFLVRKGNPKAIKDWSDLAKPGVQVVIANPKTSGN
GRYAYLAAWGFQKQKGATDQQAIDFEKAIFRNVPVLDSGGRGATTTFTQRGIGDVLVTFENEGALMDTGASGAQFDAVYP
SASILAEPPVAVVDKVVDKKGTRKVAQAYLDYLYTPEAQEIIAQHHLRPRDANVLKKHAAEFKPLKTFSVEQVFGSWANA
QKTHFADGGTFDQVIVDRK
>Mature_338_residues
ASMKGIGRWLHTGAAAALVVAATAAHADTSILNVSYDVTRELYKDINAGFAAAYKQKTGETIAIKQSHGASSAQALSVLQ
GLQADVVTMNQPNDIDLLAERGQLLPKDWRARFPDNSSPYSTTMVFLVRKGNPKAIKDWSDLAKPGVQVVIANPKTSGNG
RYAYLAAWGFQKQKGATDQQAIDFEKAIFRNVPVLDSGGRGATTTFTQRGIGDVLVTFENEGALMDTGASGAQFDAVYPS
ASILAEPPVAVVDKVVDKKGTRKVAQAYLDYLYTPEAQEIIAQHHLRPRDANVLKKHAAEFKPLKTFSVEQVFGSWANAQ
KTHFADGGTFDQVIVDRK

Specific function: This protein specifically binds sulfate and is involved in its transmembrane transport [H]

COG id: COG1613

COG function: function code P; ABC-type sulfate transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic sulfate-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI1790351, Length=330, Percent_Identity=54.5454545454545, Blast_Score=372, Evalue=1e-104,
Organism=Escherichia coli, GI1788765, Length=312, Percent_Identity=49.0384615384615, Blast_Score=307, Evalue=7e-85,

Paralogues:

None

Copy number: 1240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR000957
- InterPro:   IPR005669 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 36642; Mature: 36511

Theoretical pI: Translated: 9.49; Mature: 9.49

Prosite motif: PS00782 TFIIB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASMKGIGRWLHTGAAAALVVAATAAHADTSILNVSYDVTRELYKDINAGFAAAYKQKTG
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHCCCHHHHHHHCCC
ETIAIKQSHGASSAQALSVLQGLQADVVTMNQPNDIDLLAERGQLLPKDWRARFPDNSSP
CEEEEECCCCCCHHHHHHHHHCCCCCEEECCCCCCHHHHHHCCCCCCCHHHCCCCCCCCC
YSTTMVFLVRKGNPKAIKDWSDLAKPGVQVVIANPKTSGNGRYAYLAAWGFQKQKGATDQ
CEEEEEEEEECCCCCCHHCHHHHCCCCEEEEEECCCCCCCCCEEEEEEECCCCCCCCCHH
QAIDFEKAIFRNVPVLDSGGRGATTTFTQRGIGDVLVTFENEGALMDTGASGAQFDAVYP
HHHHHHHHHHHCCCCEECCCCCCCCHHHHCCCCEEEEEECCCCCEEECCCCCCEEEEECC
SASILAEPPVAVVDKVVDKKGTRKVAQAYLDYLYTPEAQEIIAQHHLRPRDANVLKKHAA
CHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCHHHHHHHHH
EFKPLKTFSVEQVFGSWANAQKTHFADGGTFDQVIVDRK
HCCCCCCEEHHHHHHCCCCCCCCCCCCCCCHHHEEECCC
>Mature Secondary Structure 
ASMKGIGRWLHTGAAAALVVAATAAHADTSILNVSYDVTRELYKDINAGFAAAYKQKTG
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHCCCHHHHHHHCCC
ETIAIKQSHGASSAQALSVLQGLQADVVTMNQPNDIDLLAERGQLLPKDWRARFPDNSSP
CEEEEECCCCCCHHHHHHHHHCCCCCEEECCCCCCHHHHHHCCCCCCCHHHCCCCCCCCC
YSTTMVFLVRKGNPKAIKDWSDLAKPGVQVVIANPKTSGNGRYAYLAAWGFQKQKGATDQ
CEEEEEEEEECCCCCCHHCHHHHCCCCEEEEEECCCCCCCCCEEEEEEECCCCCCCCCHH
QAIDFEKAIFRNVPVLDSGGRGATTTFTQRGIGDVLVTFENEGALMDTGASGAQFDAVYP
HHHHHHHHHHHCCCCEECCCCCCCCHHHHCCCCEEEEEECCCCCEEECCCCCCEEEEECC
SASILAEPPVAVVDKVVDKKGTRKVAQAYLDYLYTPEAQEIIAQHHLRPRDANVLKKHAA
CHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCHHHHHHHHH
EFKPLKTFSVEQVFGSWANAQKTHFADGGTFDQVIVDRK
HCCCCCCEEHHHHHHCCCCCCCCCCCCCCCHHHEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; S2O32- [Periplasm]; H2O; SO42- [Periplasm]; ATP [C]

Specific reaction: ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3158524; 2002055; 8346018; 9278503; 9298646; 8774726 [H]