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The map label for this gene is queF

Identifier: 78067534

GI number: 78067534

Start: 3207963

End: 3208787

Strand: Direct

Name: queF

Synonym: Bcep18194_A6065

Alternate gene names: 78067534

Gene position: 3207963-3208787 (Clockwise)

Preceding gene: 78067533

Following gene: 78067539

Centisome position: 86.84

GC content: 65.7

Gene sequence:

>825_bases
ATGAATCCCGAACATTCCCCGCTCGGCAAGGCCACCGTCTACGCCGCCCAGTACGACGCGTCGCTGCTGTTCCCGATCCC
GCGCGCCGGTGCGCGCGAGCAGCTCGGCATCACGTCGGCGCTGCCGTTCTTCGGCACCGACATCTGGAACGCCTACGAGC
TGTCGTGGCTCAACGCGCGCGGCAAGCCGCAGATCGCGGTTGCGACGTTCTACGTGCCGGCCGAATCGCCGAACATCGTC
GAATCGAAGTCGTTCAAGCTGTATCTCGGCTCGTTCGCGCAGACGACGTTCGACTCGATCGACGCGGTGCGCGATACGCT
GAAGCGCGACGTGTCGGCCGCATGCGGCGCGACCGTCTCCGTGCAGCTCGTGTCGCCGCACGATTTCGGCAAGCTCGAAA
TGGAAGAGCTCGACGGGCTGTCGCTCGACCGGCTCGACCTCGACACCGACGTGTACGAACCCGATCCGTCGCTGCTGAAG
GCCGCCGAGGACGAAGCGCCGGTCGAGGAGACGCTCGTATCCGACCTGCTGCGCTCGAACTGCCCGGTCACCGGCCAGCC
CGACTGGGGCAGCGTGCAGATCCACTACGTCGGGCCGCAGATCGATCACGCGGGCCTGCTGCGCTACATCATCTCGTTCC
GCAATCACACGGGCTTTCACGAGCAGTGCGTCGAGCGGATCTTCCTCGACATCCTGCACGCGTGCAAACCGGTGAAGCTC
GCGGTGTATGCGCGCTATACGCGCCGCGGCGGCCTCGACATCAATCCGTTCCGCACCAACTACAACCAGCCGATGCCGGA
CAACGCGCGCAACGCGCGGCAGTAA

Upstream 100 bases:

>100_bases
GCGCCGAATTCGAACGCTTCCTCGCGGCACTCGGCTATCCGTATGTCGAGGAGAGCGCCAACCCGGCTTACCGCCTCTTC
CTGTCGTAAAGGCGTCACGC

Downstream 100 bases:

>100_bases
CGGCGGCGGGTAGCCGCCGCGTTGTGCAGTCGGCAACGCGCGGCAACGACCCCAGACAAAACAGCCCGGCCGGGTTCAAC
CGGCCGGGCTGTTTTTCATT

Product: 7-cyano-7-deazaguanine reductase

Products: NA

Alternate protein names: 7-cyano-7-carbaguanine reductase; NADPH-dependent nitrile oxidoreductase; PreQ(0) reductase [H]

Number of amino acids: Translated: 274; Mature: 274

Protein sequence:

>274_residues
MNPEHSPLGKATVYAAQYDASLLFPIPRAGAREQLGITSALPFFGTDIWNAYELSWLNARGKPQIAVATFYVPAESPNIV
ESKSFKLYLGSFAQTTFDSIDAVRDTLKRDVSAACGATVSVQLVSPHDFGKLEMEELDGLSLDRLDLDTDVYEPDPSLLK
AAEDEAPVEETLVSDLLRSNCPVTGQPDWGSVQIHYVGPQIDHAGLLRYIISFRNHTGFHEQCVERIFLDILHACKPVKL
AVYARYTRRGGLDINPFRTNYNQPMPDNARNARQ

Sequences:

>Translated_274_residues
MNPEHSPLGKATVYAAQYDASLLFPIPRAGAREQLGITSALPFFGTDIWNAYELSWLNARGKPQIAVATFYVPAESPNIV
ESKSFKLYLGSFAQTTFDSIDAVRDTLKRDVSAACGATVSVQLVSPHDFGKLEMEELDGLSLDRLDLDTDVYEPDPSLLK
AAEDEAPVEETLVSDLLRSNCPVTGQPDWGSVQIHYVGPQIDHAGLLRYIISFRNHTGFHEQCVERIFLDILHACKPVKL
AVYARYTRRGGLDINPFRTNYNQPMPDNARNARQ
>Mature_274_residues
MNPEHSPLGKATVYAAQYDASLLFPIPRAGAREQLGITSALPFFGTDIWNAYELSWLNARGKPQIAVATFYVPAESPNIV
ESKSFKLYLGSFAQTTFDSIDAVRDTLKRDVSAACGATVSVQLVSPHDFGKLEMEELDGLSLDRLDLDTDVYEPDPSLLK
AAEDEAPVEETLVSDLLRSNCPVTGQPDWGSVQIHYVGPQIDHAGLLRYIISFRNHTGFHEQCVERIFLDILHACKPVKL
AVYARYTRRGGLDINPFRTNYNQPMPDNARNARQ

Specific function: Catalyzes the NADPH-dependent reduction of 7-cyano-7- deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1) [H]

COG id: COG0780

COG function: function code R; Enzyme related to GTP cyclohydrolase I

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP cyclohydrolase I family. QueF type 2 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789158, Length=269, Percent_Identity=53.5315985130111, Blast_Score=275, Evalue=2e-75,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016428
- InterPro:   IPR020602 [H]

Pfam domain/function: PF01227 GTP_cyclohydroI [H]

EC number: =1.7.1.13 [H]

Molecular weight: Translated: 30473; Mature: 30473

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPEHSPLGKATVYAAQYDASLLFPIPRAGAREQLGITSALPFFGTDIWNAYELSWLNAR
CCCCCCCCCCEEEEEEECCCEEEECCCCCCCHHHCCCHHCCCHHCCCCCCCEEEEEECCC
GKPQIAVATFYVPAESPNIVESKSFKLYLGSFAQTTFDSIDAVRDTLKRDVSAACGATVS
CCCCEEEEEEEEECCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHCCCEEE
VQLVSPHDFGKLEMEELDGLSLDRLDLDTDVYEPDPSLLKAAEDEAPVEETLVSDLLRSN
EEEECCCCCCCEEHHHCCCCCCCCEECCCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHCC
CPVTGQPDWGSVQIHYVGPQIDHAGLLRYIISFRNHTGFHEQCVERIFLDILHACKPVKL
CCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCEEE
AVYARYTRRGGLDINPFRTNYNQPMPDNARNARQ
EEEEEHHHCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MNPEHSPLGKATVYAAQYDASLLFPIPRAGAREQLGITSALPFFGTDIWNAYELSWLNAR
CCCCCCCCCCEEEEEEECCCEEEECCCCCCCHHHCCCHHCCCHHCCCCCCCEEEEEECCC
GKPQIAVATFYVPAESPNIVESKSFKLYLGSFAQTTFDSIDAVRDTLKRDVSAACGATVS
CCCCEEEEEEEEECCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHCCCEEE
VQLVSPHDFGKLEMEELDGLSLDRLDLDTDVYEPDPSLLKAAEDEAPVEETLVSDLLRSN
EEEECCCCCCCEEHHHCCCCCCCCEECCCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHCC
CPVTGQPDWGSVQIHYVGPQIDHAGLLRYIISFRNHTGFHEQCVERIFLDILHACKPVKL
CCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCEEE
AVYARYTRRGGLDINPFRTNYNQPMPDNARNARQ
EEEEEHHHCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA