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The map label for this gene is rluE [H]

Identifier: 78067326

GI number: 78067326

Start: 2976268

End: 2976846

Strand: Direct

Name: rluE [H]

Synonym: Bcep18194_A5857

Alternate gene names: 78067326

Gene position: 2976268-2976846 (Clockwise)

Preceding gene: 78067323

Following gene: 78067327

Centisome position: 80.57

GC content: 70.12

Gene sequence:

>579_bases
ATGAACCTGATCGCCCTCAACAAACCGTTCGGCACGATTTGCCAGTTTTCCGCGCACGAGACGCGCCCGTCGCTCGGCGA
CTGGGTAAAAACGCCCGGCGTCTACGCGGCCGGCCGGCTGGACGCGGACAGCGAGGGGCTGCTGCTGCTCACCGACGACG
GCGCGCTGCAGGCGCGCATCGCCGAGCCGCGCCACAAGCTCGTCAAGCGCTACTGGGCACAGGTCGAGGGCGCGCCCGGC
CCCACCGACCTGAAGGCGCTCGCGCGCGGCGTCGACCTCGGCGACTACGTGACGCGTCCGTGCCGCGCCGAATTCATCGA
ACCGCCCGACACGCTGTGGCCGCGCAATCCGCCGATCCGCTACCGCGCCGCGATCCCGACGACGTGGATCGAGCTTGCGA
TCACCGAGGGCAAGAACCGGCAGGTGCGCCGGATGACGGCGGCCGTCGGCTTCCCGACGTTGCGCCTGGTGCGTGTCGGC
GTCGGCGCACTGGATATATTCGCACTCGGCATTGCACCGGGAGAAACAATCGCGCTGCCGCCGCGCGCGCCGTGGGATGG
TTTCGCGCCCGCCGGATGA

Upstream 100 bases:

>100_bases
GACAGAGGACTTGCCGTTCCGTATTATGCATTAAGATTCCGCCATTTGCCATAGACCGCCCGCCCCCGCTTCCCGCGGCG
CGCCGGGCGGACGCCCCGCC

Downstream 100 bases:

>100_bases
CGAATTCGGGACGGGCGAATTTTTCGTCATCTTTTTCGTCAACCGCCTGCGAAGATCGCGCGTTAAACCACGCAGATGCC
AACCTTAGCTATCCGGCATT

Product: pseudouridine synthase, Rsu

Products: NA

Alternate protein names: rRNA pseudouridylate synthase E; rRNA-uridine isomerase E [H]

Number of amino acids: Translated: 192; Mature: 192

Protein sequence:

>192_residues
MNLIALNKPFGTICQFSAHETRPSLGDWVKTPGVYAAGRLDADSEGLLLLTDDGALQARIAEPRHKLVKRYWAQVEGAPG
PTDLKALARGVDLGDYVTRPCRAEFIEPPDTLWPRNPPIRYRAAIPTTWIELAITEGKNRQVRRMTAAVGFPTLRLVRVG
VGALDIFALGIAPGETIALPPRAPWDGFAPAG

Sequences:

>Translated_192_residues
MNLIALNKPFGTICQFSAHETRPSLGDWVKTPGVYAAGRLDADSEGLLLLTDDGALQARIAEPRHKLVKRYWAQVEGAPG
PTDLKALARGVDLGDYVTRPCRAEFIEPPDTLWPRNPPIRYRAAIPTTWIELAITEGKNRQVRRMTAAVGFPTLRLVRVG
VGALDIFALGIAPGETIALPPRAPWDGFAPAG
>Mature_192_residues
MNLIALNKPFGTICQFSAHETRPSLGDWVKTPGVYAAGRLDADSEGLLLLTDDGALQARIAEPRHKLVKRYWAQVEGAPG
PTDLKALARGVDLGDYVTRPCRAEFIEPPDTLWPRNPPIRYRAAIPTTWIELAITEGKNRQVRRMTAAVGFPTLRLVRVG
VGALDIFALGIAPGETIALPPRAPWDGFAPAG

Specific function: Responsible for synthesis of pseudouridine from uracil- 2457 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase rsuA family [H]

Homologues:

Organism=Escherichia coli, GI87081838, Length=170, Percent_Identity=52.9411764705882, Blast_Score=176, Evalue=6e-46,
Organism=Escherichia coli, GI1788510, Length=175, Percent_Identity=34.2857142857143, Blast_Score=86, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496 [H]

Pfam domain/function: PF00849 PseudoU_synth_2 [H]

EC number: NA

Molecular weight: Translated: 20872; Mature: 20872

Theoretical pI: Translated: 9.17; Mature: 9.17

Prosite motif: PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLIALNKPFGTICQFSAHETRPSLGDWVKTPGVYAAGRLDADSEGLLLLTDDGALQARI
CCEEEECCCCCHHEECCCCCCCCCCHHHHCCCCCEEECCCCCCCCCEEEEECCCCEEEEC
AEPRHKLVKRYWAQVEGAPGPTDLKALARGVDLGDYVTRPCRAEFIEPPDTLWPRNPPIR
CCHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCHHHHCCCCCCCCCCCCCCCCCCCCCEE
YRAAIPTTWIELAITEGKNRQVRRMTAAVGFPTLRLVRVGVGALDIFALGIAPGETIALP
EEEECCCEEEEEEEECCCCCHHHHHHHHHCCCHHHHHHHCCCHHHEEEEECCCCCEEECC
PRAPWDGFAPAG
CCCCCCCCCCCC
>Mature Secondary Structure
MNLIALNKPFGTICQFSAHETRPSLGDWVKTPGVYAAGRLDADSEGLLLLTDDGALQARI
CCEEEECCCCCHHEECCCCCCCCCCHHHHCCCCCEEECCCCCCCCCEEEEECCCCEEEEC
AEPRHKLVKRYWAQVEGAPGPTDLKALARGVDLGDYVTRPCRAEFIEPPDTLWPRNPPIR
CCHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCHHHHCCCCCCCCCCCCCCCCCCCCCEE
YRAAIPTTWIELAITEGKNRQVRRMTAAVGFPTLRLVRVGVGALDIFALGIAPGETIALP
EEEECCCEEEEEEEECCCCCHHHHHHHHHCCCHHHHHHHCCCHHHEEEEECCCCCEEECC
PRAPWDGFAPAG
CCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]