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The map label for this gene is ispH

Identifier: 78067300

GI number: 78067300

Start: 2945430

End: 2946410

Strand: Reverse

Name: ispH

Synonym: Bcep18194_A5831

Alternate gene names: 78067300

Gene position: 2946410-2945430 (Counterclockwise)

Preceding gene: 78067301

Following gene: 78067299

Centisome position: 79.76

GC content: 66.26

Gene sequence:

>981_bases
ATGAGCACCACCGATACGCTGTCCGGGCAGACCGTCGCCGCCGATGCCGAAATCCTGCTGGCCCAGCCGCGCGGTTTCTG
CGCGGGCGTCGACCGCGCGATCGAGATCGTCGAGCGCGCCATCGCGATGCACGGTGCGCCGATCTACGTTCGTCACGAGA
TCGTCCACAACAAGTACGTGGTCGAGGATCTGAAGAAGAAAGGCGCGATCTTCGTCGAGGAACTCGAGGAAGTGCCGGCC
GGCAATACCGTGATCTTCAGTGCGCACGGCGTGTCGAAGGCCGTGCGCGACGAGGCCGACGTGCGCGGGTTGCGCATTTA
CGACGCAACCTGCCCGCTCGTCACGAAGGTGCACGTCGAAGTGGCGAAGATGCGCCAGGACGGCGTCGACATCGTCATGA
TCGGGCACAAGGGCCATCCGGAAGTCGAAGGCACGATGGGGCAGGTCGAGCGCGGCATGCATCTCGTCGAGAGCGTCGAG
GACGTGCAGAAGCTCGAGCTCGCCGATCCCGAGCGCATCGCGCTCGTCACGCAGACCACGCTGTCCGTCGACGACGCAGC
CGAGATCATCGGCGCGTTGAAGGCCAAGTACCCGAAGATCCGCGAGCCGAAGAAGCAGGACATCTGCTACGCGACGCAGA
ACCGCCAGGACGCGGTGAAGTTCATGGCGCCGCAGTGCGATGTCGTGATCGTCGTCGGCAGCCCGAACAGCTCGAATTCG
AGCCGCCTGCGCGAAGTGGCCGAGAAGCGCGGCGTCGACGCGTACATGGTCGATGCGCCCGACCAGATCGATCCGGCATG
GGTCGCAGGCAAGCGTCGCATCGGCGTGACAGCCGGCGCGTCGGCGCCCGAGGTGCTCGCCCAGGCCGTGATCGCGCGAC
TGCGCGAGCTCGGCGTGCGCAATGTGCGCGCGCTCGACGGCATCGAGGAAAACGTGTCGTTTCCGCTGCCGCGCGGGTTG
AACCTGCCGCCCGCTGCCTGA

Upstream 100 bases:

>100_bases
TGAAGGAAGTCAGCGAAACCTCGGCACTGTTCGACTTCAATCATCCGCTCGCGGGCCAAGCGCTCACGTTCGAAGTGAAA
ATCATCGGAATCCTGTAATC

Downstream 100 bases:

>100_bases
TTGACGCATCGCACCTGCGGAACACCGAAGCGCGCCTTACGGCGCGCTTTTTTTTGCCTGTCGAAATTGCAATCGCAACC
ATTATTCGGATTAATGGTGC

Product: 4-hydroxy-3-methylbut-2-enyl diphosphate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 326; Mature: 325

Protein sequence:

>326_residues
MSTTDTLSGQTVAADAEILLAQPRGFCAGVDRAIEIVERAIAMHGAPIYVRHEIVHNKYVVEDLKKKGAIFVEELEEVPA
GNTVIFSAHGVSKAVRDEADVRGLRIYDATCPLVTKVHVEVAKMRQDGVDIVMIGHKGHPEVEGTMGQVERGMHLVESVE
DVQKLELADPERIALVTQTTLSVDDAAEIIGALKAKYPKIREPKKQDICYATQNRQDAVKFMAPQCDVVIVVGSPNSSNS
SRLREVAEKRGVDAYMVDAPDQIDPAWVAGKRRIGVTAGASAPEVLAQAVIARLRELGVRNVRALDGIEENVSFPLPRGL
NLPPAA

Sequences:

>Translated_326_residues
MSTTDTLSGQTVAADAEILLAQPRGFCAGVDRAIEIVERAIAMHGAPIYVRHEIVHNKYVVEDLKKKGAIFVEELEEVPA
GNTVIFSAHGVSKAVRDEADVRGLRIYDATCPLVTKVHVEVAKMRQDGVDIVMIGHKGHPEVEGTMGQVERGMHLVESVE
DVQKLELADPERIALVTQTTLSVDDAAEIIGALKAKYPKIREPKKQDICYATQNRQDAVKFMAPQCDVVIVVGSPNSSNS
SRLREVAEKRGVDAYMVDAPDQIDPAWVAGKRRIGVTAGASAPEVLAQAVIARLRELGVRNVRALDGIEENVSFPLPRGL
NLPPAA
>Mature_325_residues
STTDTLSGQTVAADAEILLAQPRGFCAGVDRAIEIVERAIAMHGAPIYVRHEIVHNKYVVEDLKKKGAIFVEELEEVPAG
NTVIFSAHGVSKAVRDEADVRGLRIYDATCPLVTKVHVEVAKMRQDGVDIVMIGHKGHPEVEGTMGQVERGMHLVESVED
VQKLELADPERIALVTQTTLSVDDAAEIIGALKAKYPKIREPKKQDICYATQNRQDAVKFMAPQCDVVIVVGSPNSSNSS
RLREVAEKRGVDAYMVDAPDQIDPAWVAGKRRIGVTAGASAPEVLAQAVIARLRELGVRNVRALDGIEENVSFPLPRGLN
LPPAA

Specific function: Converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP) [H]

COG id: COG0761

COG function: function code IM; Penicillin tolerance protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispH family [H]

Homologues:

Organism=Escherichia coli, GI1786212, Length=309, Percent_Identity=57.6051779935275, Blast_Score=369, Evalue=1e-103,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003451 [H]

Pfam domain/function: PF02401 LYTB [H]

EC number: =1.17.1.2 [H]

Molecular weight: Translated: 35317; Mature: 35186

Theoretical pI: Translated: 5.67; Mature: 5.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTTDTLSGQTVAADAEILLAQPRGFCAGVDRAIEIVERAIAMHGAPIYVRHEIVHNKYV
CCCCCCCCCCEEECCCEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHH
VEDLKKKGAIFVEELEEVPAGNTVIFSAHGVSKAVRDEADVRGLRIYDATCPLVTKVHVE
HHHHHHCCCEEHHHHHHCCCCCEEEEECCCCHHHHHHHHCCCCEEEEECCCCCHHHHHHH
VAKMRQDGVDIVMIGHKGHPEVEGTMGQVERGMHLVESVEDVQKLELADPERIALVTQTT
HHHHHHCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEE
LSVDDAAEIIGALKAKYPKIREPKKQDICYATQNRQDAVKFMAPQCDVVIVVGSPNSSNS
CCHHHHHHHHHHHHHCCCCCCCCCCCCCEEECCCHHHHHHHHCCCCCEEEEEECCCCCCH
SRLREVAEKRGVDAYMVDAPDQIDPAWVAGKRRIGVTAGASAPEVLAQAVIARLRELGVR
HHHHHHHHHCCCCEEEECCCCCCCCHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCC
NVRALDGIEENVSFPLPRGLNLPPAA
HHHHHCCCHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
STTDTLSGQTVAADAEILLAQPRGFCAGVDRAIEIVERAIAMHGAPIYVRHEIVHNKYV
CCCCCCCCCEEECCCEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHH
VEDLKKKGAIFVEELEEVPAGNTVIFSAHGVSKAVRDEADVRGLRIYDATCPLVTKVHVE
HHHHHHCCCEEHHHHHHCCCCCEEEEECCCCHHHHHHHHCCCCEEEEECCCCCHHHHHHH
VAKMRQDGVDIVMIGHKGHPEVEGTMGQVERGMHLVESVEDVQKLELADPERIALVTQTT
HHHHHHCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEE
LSVDDAAEIIGALKAKYPKIREPKKQDICYATQNRQDAVKFMAPQCDVVIVVGSPNSSNS
CCHHHHHHHHHHHHHCCCCCCCCCCCCCEEECCCHHHHHHHHCCCCCEEEEEECCCCCCH
SRLREVAEKRGVDAYMVDAPDQIDPAWVAGKRRIGVTAGASAPEVLAQAVIARLRELGVR
HHHHHHHHHCCCCEEEECCCCCCCCHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCC
NVRALDGIEENVSFPLPRGLNLPPAA
HHHHHCCCHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA