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The map label for this gene is btuF [H]

Identifier: 78067250

GI number: 78067250

Start: 2896621

End: 2897535

Strand: Direct

Name: btuF [H]

Synonym: Bcep18194_A5781

Alternate gene names: 78067250

Gene position: 2896621-2897535 (Clockwise)

Preceding gene: 78067249

Following gene: 78067256

Centisome position: 78.41

GC content: 70.27

Gene sequence:

>915_bases
ATGAATCCGCTCATGGTTCGCCGGCTCGCGCCAGTCGCGTTACTGGCCGCACTCGCGTACGCACCACTCGTCCGCGCCGA
TGTCACAACGCGCGACGACGCCGGCAATACGGTCACACTGCCGGCGCCCGCGCAGCGCGTGATCAGCCTCGCGCCGCACG
CGACCGAGCTGGTCTACGCGGCCGGCGGCGGAGCAAAGCTGGTCGGCACCGTCACGTACAGCGACTATCCGCCCGCCGTG
CAGGCCGTACCGCGCGTCGGCGACAACAAGGCGCTCGATCTCGAGCGGATCGCCGCACTGAAGCCCGACCTGATCGTCGT
CTGGCGCCACGGCAATGCCGAGCGGCAAACGGACGCGCTGCGTGCGTTGCACATCCCGCTGTTCCTCAGCGAACCGAAAC
ATCTCGACGACGTATCGTCGTCACTGCGCCGGCTCGGCACGCTGCTCGGCACGCAACCGGCTGCCGATACGGCCGCGGCC
GCCTACACGCGCGACATCGCGGCGCTGCGCACGCGTTACGCGGCGCGAGCGCCGGTCACGATGTTCTTCCAGGTCTGGGA
TCGGCCGCTGATGACGCTCAACGGTGCGCACCTGATCAACGACGTGATCGAGCTGTGCGGCGGGCGCAACGTGTTCGCCT
CGCTCAAGCCGCTCGTGCCGACCGTCACCGACGAGGCCGTGCTCGCGGCGAATCCGGAGGCGATCGTGACGACCAGCGCG
GGTACGACGCGCTCCGACGAACCGCTGCCGAGCCTGGCGCGCTGGCGCGCGTGGCCCGCCCTGACGGCCGTGGCCCGCAA
CAACCTGTTCGCGATCGATGGCGATCTGCTGACACGGCCATCGCCGCGGATCGCGCAGGGTGCGGCTGCGCTTTGCGAGG
ATCTCGATGCCGCGCGTGCGCGGCGGCCGCGCTGA

Upstream 100 bases:

>100_bases
CATCGAGCGTACGCGTCGGTCTGCCGGGCAGCGAAGCCGAATGGCAGCGCCTCGGCGACGCGCTCGCGCACTGCGTGCCG
ACGCTGCAACAGGAATCCGC

Downstream 100 bases:

>100_bases
TTTCCGGCGGCTTACGCGTTCATCGCGTGTTGGATGCCTGAGACGAATCGACCGCGCGCTGCATTGTTCACACGTCGTGG
CCAGCGCGCCTATTCGTCCC

Product: ABC Fe3+siderophore/cobalamin transporter, periplasmic ligand binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 304; Mature: 304

Protein sequence:

>304_residues
MNPLMVRRLAPVALLAALAYAPLVRADVTTRDDAGNTVTLPAPAQRVISLAPHATELVYAAGGGAKLVGTVTYSDYPPAV
QAVPRVGDNKALDLERIAALKPDLIVVWRHGNAERQTDALRALHIPLFLSEPKHLDDVSSSLRRLGTLLGTQPAADTAAA
AYTRDIAALRTRYAARAPVTMFFQVWDRPLMTLNGAHLINDVIELCGGRNVFASLKPLVPTVTDEAVLAANPEAIVTTSA
GTTRSDEPLPSLARWRAWPALTAVARNNLFAIDGDLLTRPSPRIAQGAAALCEDLDAARARRPR

Sequences:

>Translated_304_residues
MNPLMVRRLAPVALLAALAYAPLVRADVTTRDDAGNTVTLPAPAQRVISLAPHATELVYAAGGGAKLVGTVTYSDYPPAV
QAVPRVGDNKALDLERIAALKPDLIVVWRHGNAERQTDALRALHIPLFLSEPKHLDDVSSSLRRLGTLLGTQPAADTAAA
AYTRDIAALRTRYAARAPVTMFFQVWDRPLMTLNGAHLINDVIELCGGRNVFASLKPLVPTVTDEAVLAANPEAIVTTSA
GTTRSDEPLPSLARWRAWPALTAVARNNLFAIDGDLLTRPSPRIAQGAAALCEDLDAARARRPR
>Mature_304_residues
MNPLMVRRLAPVALLAALAYAPLVRADVTTRDDAGNTVTLPAPAQRVISLAPHATELVYAAGGGAKLVGTVTYSDYPPAV
QAVPRVGDNKALDLERIAALKPDLIVVWRHGNAERQTDALRALHIPLFLSEPKHLDDVSSSLRRLGTLLGTQPAADTAAA
AYTRDIAALRTRYAARAPVTMFFQVWDRPLMTLNGAHLINDVIELCGGRNVFASLKPLVPTVTDEAVLAANPEAIVTTSA
GTTRSDEPLPSLARWRAWPALTAVARNNLFAIDGDLLTRPSPRIAQGAAALCEDLDAARARRPR

Specific function: Part of the ABC transporter complex BtuCDF involved in vitamin B12 import. Binds vitamin B12 and delivers it to the periplasmic surface of BtuC [H]

COG id: COG0614

COG function: function code P; ABC-type Fe3+-hydroxamate transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Fe/B12 periplasmic-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786353, Length=252, Percent_Identity=35.3174603174603, Blast_Score=147, Evalue=1e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002491 [H]

Pfam domain/function: PF01497 Peripla_BP_2 [H]

EC number: NA

Molecular weight: Translated: 32515; Mature: 32515

Theoretical pI: Translated: 9.45; Mature: 9.45

Prosite motif: PS50983 FE_B12_PBP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPLMVRRLAPVALLAALAYAPLVRADVTTRDDAGNTVTLPAPAQRVISLAPHATELVYA
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCCHHHHHHCCCCHHEEEEE
AGGGAKLVGTVTYSDYPPAVQAVPRVGDNKALDLERIAALKPDLIVVWRHGNAERQTDAL
CCCCCEEEEEEEECCCCCHHHHHCCCCCCCCCCHHHHHHCCCCEEEEECCCCCHHHHHHH
RALHIPLFLSEPKHLDDVSSSLRRLGTLLGTQPAADTAAAAYTRDIAALRTRYAARAPVT
HHHCCEEEECCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHH
MFFQVWDRPLMTLNGAHLINDVIELCGGRNVFASLKPLVPTVTDEAVLAANPEAIVTTSA
HHHHHHCCHHEEECHHHHHHHHHHHHCCCCHHHHHCCCCCCCCCCEEEECCCCEEEEECC
GTTRSDEPLPSLARWRAWPALTAVARNNLFAIDGDLLTRPSPRIAQGAAALCEDLDAARA
CCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHH
RRPR
CCCC
>Mature Secondary Structure
MNPLMVRRLAPVALLAALAYAPLVRADVTTRDDAGNTVTLPAPAQRVISLAPHATELVYA
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCCHHHHHHCCCCHHEEEEE
AGGGAKLVGTVTYSDYPPAVQAVPRVGDNKALDLERIAALKPDLIVVWRHGNAERQTDAL
CCCCCEEEEEEEECCCCCHHHHHCCCCCCCCCCHHHHHHCCCCEEEEECCCCCHHHHHHH
RALHIPLFLSEPKHLDDVSSSLRRLGTLLGTQPAADTAAAAYTRDIAALRTRYAARAPVT
HHHCCEEEECCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHH
MFFQVWDRPLMTLNGAHLINDVIELCGGRNVFASLKPLVPTVTDEAVLAANPEAIVTTSA
HHHHHHCCHHEEECHHHHHHHHHHHHCCCCHHHHHCCCCCCCCCCEEEECCCCEEEEECC
GTTRSDEPLPSLARWRAWPALTAVARNNLFAIDGDLLTRPSPRIAQGAAALCEDLDAARA
CCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHH
RRPR
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA