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The map label for this gene is ilvI [H]

Identifier: 78067061

GI number: 78067061

Start: 2690592

End: 2692355

Strand: Reverse

Name: ilvI [H]

Synonym: Bcep18194_A5592

Alternate gene names: 78067061

Gene position: 2692355-2690592 (Counterclockwise)

Preceding gene: 78067073

Following gene: 78067060

Centisome position: 72.88

GC content: 62.02

Gene sequence:

>1764_bases
ATGAACATGCCCAGCGCGGAATTCTCCACGTCGGAACCCCTTTCCCCTCCCGATAGCGACTCCATCGGCGGTACCGTGCT
CATGAAGGCACTGGCCGATGAAAACGTCGAATTCATCTGGGGCTACCCCGGCGGCTCGGTACTCTACATCTACGACGAGC
TTTACAAGCAGGACAAGATTCAGCACGTGCTGGTGCGCCATGAACAGGCGGCCGTGCACGCAGCCGATGCGTATGCGCGT
TCCACCGGCAATGTCGGCGTCTGTCTCGTGACGTCGGGCCCCGGTGTCACCAATGCGGTGACCGGCATCGCCACGGCGTA
CATGGATTCGATCCCGATGGTCGTGATCAGCGGCCAGGTGCCCACGGCCGCGATCGGCCAGGATGCATTCCAGGAGTGCG
ACACCGTCGGCATCACGCGTCCGTGCGTGAAGCACAACTTCCTCGTGAAGGACGTGCGCGACCTCGCGGAAACCGTCAAG
AAGGCGTTTTATATCGCCCGCACCGGCCGTCCGGGCCCGGTGCTGATCGACATCCCGAAGGACATCTCGAAGACGCCGTG
CGAATACGAGCCCGTCAAGAGCGTGTCGCTGCGTTCGTACAACCCGGTCACGAAGGGCCATTCGGGCCAGATCCGCAAGG
CCGTGTCGCTGCTGCTGACCGCGAAGCGTCCGTACATCTACACGGGTGGCGGCATCATCCTCGCCGACGCGTCGCGTGAA
CTGAACCAGTTCGCGGACCTGCTCGGCTACCCGGTCACCAACACGCTGATGGGCCTCGGCGGCTATCGCGCGTCGGACAA
GAAATTCCTCGGCATGCTCGGCATGCACGGCACCTACGAAGCGAACATGGCGATGCAGCACTGCGACGTGCTGATCGCGA
TCGGTGCCCGCTTCGATGACCGCGTGATCGGCGACCCGGCGCACTTCGCGTCGCGTCCGCGCAAGATCATCCACATCGAC
ATCGACCCGTCGTCGATCTCGAAGCGCGTGAAGGTCGATATCCCGATCGTCGGCGACGTGAAGGAAGTGCTGAAGGAACT
GATCGAGCAACTGCAGACGGCCGAGCATGGCCCCGACACCGAGGCGCTCGCGCAATGGTGGAAGGACATCGAGGGCTGGC
GCTCGAAGGACTGCCTGAAATACGACCGCGAAAGCGAGATCATCAAGCCGCAATACGTGGTGGAGAAGGCGTGGGAGCTG
ACGGACGGCAATGCGTTCGTGTGCTCGGACGTCGGCCAGCACCAGATGTGGGCGGCGCAGTTCTACCGTTTCAACAAGCC
GCGTCGCTGGATCAACTCCGGCGGCCTCGGCACGATGGGCTTCGGCCTGCCGGCAGCGATGGGCGTCAAGATGGCGCACC
CGGACGACGACGTGCTGTGCATCACGGGCGAAGGCTCGATCCAGATGTGCATACAGGAACTGTCGACCTGCCTGCAGTAC
GACACGCCCGTGAAGATCATTTCGCTGAACAACCGCTATCTCGGCATGGTTCGCCAGTGGCAGCAGATCGAATACAGCAA
GCGCTATTCGCATTCGTACATGGATGCGCTGCCTGATTTCGTGAAGCTTGCCGAAGCGTACGGCCATGTCGGCTTGCGGA
TCGAAAAGACCTCGGATGTGGAGCCGGCGCTGAAGGAAGCGCTGCGCCTGAAGGATCGCACCGTGTTTCTCGACTTCCAG
ACTGATCCGACCGAAAACGTCTGGCCGATGGTACAGGCCGGCAAGGGCATCACCGAGATGCTGCTCGGATCGGAAGATCT
GTAA

Upstream 100 bases:

>100_bases
AGAAGCCAGCCCTTCAAACTGGACGCGCCGCTTGAACCCGAGCCACAAGCCCGGCAGAGAGCACCCGATCAATTTTTTGC
CGAAAATTCGAAAGGTCAAA

Downstream 100 bases:

>100_bases
CGGCGCGACGCGCGCCTGAGCCCGATGCGGCCGCCTTCACGAAGGGCGGTGCGGCACGCGGCAGCGCGCGGCGCGAGTGA
ATCGACACGGACACATTCTG

Product: acetolactate synthase 3 catalytic subunit

Products: NA

Alternate protein names: AHAS-III; ALS-III; Acetohydroxy-acid synthase III large subunit [H]

Number of amino acids: Translated: 587; Mature: 587

Protein sequence:

>587_residues
MNMPSAEFSTSEPLSPPDSDSIGGTVLMKALADENVEFIWGYPGGSVLYIYDELYKQDKIQHVLVRHEQAAVHAADAYAR
STGNVGVCLVTSGPGVTNAVTGIATAYMDSIPMVVISGQVPTAAIGQDAFQECDTVGITRPCVKHNFLVKDVRDLAETVK
KAFYIARTGRPGPVLIDIPKDISKTPCEYEPVKSVSLRSYNPVTKGHSGQIRKAVSLLLTAKRPYIYTGGGIILADASRE
LNQFADLLGYPVTNTLMGLGGYRASDKKFLGMLGMHGTYEANMAMQHCDVLIAIGARFDDRVIGDPAHFASRPRKIIHID
IDPSSISKRVKVDIPIVGDVKEVLKELIEQLQTAEHGPDTEALAQWWKDIEGWRSKDCLKYDRESEIIKPQYVVEKAWEL
TDGNAFVCSDVGQHQMWAAQFYRFNKPRRWINSGGLGTMGFGLPAAMGVKMAHPDDDVLCITGEGSIQMCIQELSTCLQY
DTPVKIISLNNRYLGMVRQWQQIEYSKRYSHSYMDALPDFVKLAEAYGHVGLRIEKTSDVEPALKEALRLKDRTVFLDFQ
TDPTENVWPMVQAGKGITEMLLGSEDL

Sequences:

>Translated_587_residues
MNMPSAEFSTSEPLSPPDSDSIGGTVLMKALADENVEFIWGYPGGSVLYIYDELYKQDKIQHVLVRHEQAAVHAADAYAR
STGNVGVCLVTSGPGVTNAVTGIATAYMDSIPMVVISGQVPTAAIGQDAFQECDTVGITRPCVKHNFLVKDVRDLAETVK
KAFYIARTGRPGPVLIDIPKDISKTPCEYEPVKSVSLRSYNPVTKGHSGQIRKAVSLLLTAKRPYIYTGGGIILADASRE
LNQFADLLGYPVTNTLMGLGGYRASDKKFLGMLGMHGTYEANMAMQHCDVLIAIGARFDDRVIGDPAHFASRPRKIIHID
IDPSSISKRVKVDIPIVGDVKEVLKELIEQLQTAEHGPDTEALAQWWKDIEGWRSKDCLKYDRESEIIKPQYVVEKAWEL
TDGNAFVCSDVGQHQMWAAQFYRFNKPRRWINSGGLGTMGFGLPAAMGVKMAHPDDDVLCITGEGSIQMCIQELSTCLQY
DTPVKIISLNNRYLGMVRQWQQIEYSKRYSHSYMDALPDFVKLAEAYGHVGLRIEKTSDVEPALKEALRLKDRTVFLDFQ
TDPTENVWPMVQAGKGITEMLLGSEDL
>Mature_587_residues
MNMPSAEFSTSEPLSPPDSDSIGGTVLMKALADENVEFIWGYPGGSVLYIYDELYKQDKIQHVLVRHEQAAVHAADAYAR
STGNVGVCLVTSGPGVTNAVTGIATAYMDSIPMVVISGQVPTAAIGQDAFQECDTVGITRPCVKHNFLVKDVRDLAETVK
KAFYIARTGRPGPVLIDIPKDISKTPCEYEPVKSVSLRSYNPVTKGHSGQIRKAVSLLLTAKRPYIYTGGGIILADASRE
LNQFADLLGYPVTNTLMGLGGYRASDKKFLGMLGMHGTYEANMAMQHCDVLIAIGARFDDRVIGDPAHFASRPRKIIHID
IDPSSISKRVKVDIPIVGDVKEVLKELIEQLQTAEHGPDTEALAQWWKDIEGWRSKDCLKYDRESEIIKPQYVVEKAWEL
TDGNAFVCSDVGQHQMWAAQFYRFNKPRRWINSGGLGTMGFGLPAAMGVKMAHPDDDVLCITGEGSIQMCIQELSTCLQY
DTPVKIISLNNRYLGMVRQWQQIEYSKRYSHSYMDALPDFVKLAEAYGHVGLRIEKTSDVEPALKEALRLKDRTVFLDFQ
TDPTENVWPMVQAGKGITEMLLGSEDL

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=583, Percent_Identity=25.0428816466552, Blast_Score=171, Evalue=1e-42,
Organism=Homo sapiens, GI21361361, Length=571, Percent_Identity=26.9702276707531, Blast_Score=162, Evalue=6e-40,
Organism=Escherichia coli, GI87081685, Length=571, Percent_Identity=57.7933450087566, Blast_Score=680, Evalue=0.0,
Organism=Escherichia coli, GI1790104, Length=560, Percent_Identity=40, Blast_Score=409, Evalue=1e-115,
Organism=Escherichia coli, GI1786717, Length=534, Percent_Identity=34.2696629213483, Blast_Score=315, Evalue=6e-87,
Organism=Escherichia coli, GI1787096, Length=563, Percent_Identity=28.2415630550622, Blast_Score=205, Evalue=5e-54,
Organism=Escherichia coli, GI1788716, Length=554, Percent_Identity=24.0072202166065, Blast_Score=147, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI17531301, Length=550, Percent_Identity=26, Blast_Score=158, Evalue=8e-39,
Organism=Caenorhabditis elegans, GI17531299, Length=550, Percent_Identity=26, Blast_Score=158, Evalue=8e-39,
Organism=Caenorhabditis elegans, GI17542570, Length=510, Percent_Identity=27.0588235294118, Blast_Score=144, Evalue=9e-35,
Organism=Saccharomyces cerevisiae, GI6323755, Length=592, Percent_Identity=42.5675675675676, Blast_Score=459, Evalue=1e-130,
Organism=Saccharomyces cerevisiae, GI6320816, Length=475, Percent_Identity=24.4210526315789, Blast_Score=102, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6321524, Length=527, Percent_Identity=20.303605313093, Blast_Score=81, Evalue=4e-16,
Organism=Saccharomyces cerevisiae, GI6323163, Length=533, Percent_Identity=21.9512195121951, Blast_Score=80, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6323073, Length=490, Percent_Identity=21.6326530612245, Blast_Score=73, Evalue=1e-13,
Organism=Drosophila melanogaster, GI19922626, Length=565, Percent_Identity=25.6637168141593, Blast_Score=179, Evalue=6e-45,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 64951; Mature: 64951

Theoretical pI: Translated: 5.91; Mature: 5.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNMPSAEFSTSEPLSPPDSDSIGGTVLMKALADENVEFIWGYPGGSVLYIYDELYKQDKI
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEECCCCCEEEEEHHHHHHHHH
QHVLVRHEQAAVHAADAYARSTGNVGVCLVTSGPGVTNAVTGIATAYMDSIPMVVISGQV
HHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCCEEEEECCC
PTAAIGQDAFQECDTVGITRPCVKHNFLVKDVRDLAETVKKAFYIARTGRPGPVLIDIPK
CCHHHCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCEEEECCC
DISKTPCEYEPVKSVSLRSYNPVTKGHSGQIRKAVSLLLTAKRPYIYTGGGIILADASRE
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEECCCEEEECCHHH
LNQFADLLGYPVTNTLMGLGGYRASDKKFLGMLGMHGTYEANMAMQHCDVLIAIGARFDD
HHHHHHHHCCCHHHHHHHCCCCCCCCHHHHHHHCCCCCCHHHHHHHHCCEEEEECCCCCC
RVIGDPAHFASRPRKIIHIDIDPSSISKRVKVDIPIVGDVKEVLKELIEQLQTAEHGPDT
CCCCCCHHHHCCCCEEEEEEECHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCH
EALAQWWKDIEGWRSKDCLKYDRESEIIKPQYVVEKAWELTDGNAFVCSDVGQHQMWAAQ
HHHHHHHHHHCCCCCCCHHHCCCCCCCCCHHHHHHHHHCCCCCCEEEECCCCCHHHHHHH
FYRFNKPRRWINSGGLGTMGFGLPAAMGVKMAHPDDDVLCITGEGSIQMCIQELSTCLQY
HHHCCCCHHHHCCCCCCCCCCCCHHHHCEEEECCCCCEEEEECCCHHHHHHHHHHHHHCC
DTPVKIISLNNRYLGMVRQWQQIEYSKRYSHSYMDALPDFVKLAEAYGHVGLRIEKTSDV
CCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCC
EPALKEALRLKDRTVFLDFQTDPTENVWPMVQAGKGITEMLLGSEDL
CHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHCCCCHHHHHCCCCCC
>Mature Secondary Structure
MNMPSAEFSTSEPLSPPDSDSIGGTVLMKALADENVEFIWGYPGGSVLYIYDELYKQDKI
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEECCCCCEEEEEHHHHHHHHH
QHVLVRHEQAAVHAADAYARSTGNVGVCLVTSGPGVTNAVTGIATAYMDSIPMVVISGQV
HHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCCEEEEECCC
PTAAIGQDAFQECDTVGITRPCVKHNFLVKDVRDLAETVKKAFYIARTGRPGPVLIDIPK
CCHHHCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCEEEECCC
DISKTPCEYEPVKSVSLRSYNPVTKGHSGQIRKAVSLLLTAKRPYIYTGGGIILADASRE
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEECCCEEEECCHHH
LNQFADLLGYPVTNTLMGLGGYRASDKKFLGMLGMHGTYEANMAMQHCDVLIAIGARFDD
HHHHHHHHCCCHHHHHHHCCCCCCCCHHHHHHHCCCCCCHHHHHHHHCCEEEEECCCCCC
RVIGDPAHFASRPRKIIHIDIDPSSISKRVKVDIPIVGDVKEVLKELIEQLQTAEHGPDT
CCCCCCHHHHCCCCEEEEEEECHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCH
EALAQWWKDIEGWRSKDCLKYDRESEIIKPQYVVEKAWELTDGNAFVCSDVGQHQMWAAQ
HHHHHHHHHHCCCCCCCHHHCCCCCCCCCHHHHHHHHHCCCCCCEEEECCCCCHHHHHHH
FYRFNKPRRWINSGGLGTMGFGLPAAMGVKMAHPDDDVLCITGEGSIQMCIQELSTCLQY
HHHCCCCHHHHCCCCCCCCCCCCHHHHCEEEECCCCCEEEEECCCHHHHHHHHHHHHHCC
DTPVKIISLNNRYLGMVRQWQQIEYSKRYSHSYMDALPDFVKLAEAYGHVGLRIEKTSDV
CCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCC
EPALKEALRLKDRTVFLDFQTDPTENVWPMVQAGKGITEMLLGSEDL
CHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHCCCCHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6308579; 1630901; 9278503; 3891724; 9298646 [H]