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The map label for this gene is rhtB [H]

Identifier: 78066983

GI number: 78066983

Start: 2606437

End: 2607066

Strand: Reverse

Name: rhtB [H]

Synonym: Bcep18194_A5514

Alternate gene names: 78066983

Gene position: 2607066-2606437 (Counterclockwise)

Preceding gene: 78066987

Following gene: 78066982

Centisome position: 70.57

GC content: 66.51

Gene sequence:

>630_bases
ATGTCGCTGTCCGCCTTGCTCGCTTTTGCCCTGATCCTGTCCGTCGGCGTTGCGACGCCCGGCCCGACGGTGTTGCTCGC
AATGAGCAACGGCTCGCGGTACGGATTGCGTCACGCGACGGTCGGCATGCTCGGCGCCGTCACGGCCGATGTCGTGCTCG
TTGCGCTGGTCGGGTTCGGTCTAGGGATGCTGCTCGACGCGTCGGAAACGGCATTCGTCACGCTGAAGCTGGCCGGTGCC
GCGTGGCTCGCCTATGTCGGCGTGCGGATGCTGCTGTCGTCCGGCGGTTCTGCCGCTGCACCGTCGCTCGAGCACGCGAC
GCCCGATCGCCGGGCCGCGTTCCTGAAGAGCTTCTTCGTTGCAATGAGCAACCCGAAGTACTACCTGTTCATGTCCGCGC
TGCTGCCGCAGTTCGTCGACCGGTCGCATGCGATCGCGCCGCAGTACGCGATCCTCGCGGCGACGATCGTCGCGATCGAT
GTCGTCGGGATGACCGGCTATGCGCTGCTCGGCGTGCATTCGGTGCGTGTGTGGAAAGCGGCGGGGGAGAAGTGGCTGAA
CCGCGTCAGCGGGTCGCTGCTGCTGATGCTCGCGGGGTACGTCGCGCTGTATCGCAAGGCCGCGAACTGA

Upstream 100 bases:

>100_bases
AATTCGTCGTAACGATGCGTGCGTGCCGGCAGCGGCCCGCATGGCCGAAGACGGCGACGCCACCCCGTACGCAAGCTCCC
TTCAGAAGGAACGCCACGCC

Downstream 100 bases:

>100_bases
CGCGGCGATGCGTCGGGAGCAAGCCCCTCGCCAACCGGCTCGGCGATGAAGTCGATCGAGCGCTTCACGCTCGAAACGCA
CGACGGTCCGTATGAAGCGT

Product: lysine exporter protein LysE/YggA

Products: homoserine lactone [Periplasm]; Proton [Cytoplasm]; homoserine [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 209; Mature: 208

Protein sequence:

>209_residues
MSLSALLAFALILSVGVATPGPTVLLAMSNGSRYGLRHATVGMLGAVTADVVLVALVGFGLGMLLDASETAFVTLKLAGA
AWLAYVGVRMLLSSGGSAAAPSLEHATPDRRAAFLKSFFVAMSNPKYYLFMSALLPQFVDRSHAIAPQYAILAATIVAID
VVGMTGYALLGVHSVRVWKAAGEKWLNRVSGSLLLMLAGYVALYRKAAN

Sequences:

>Translated_209_residues
MSLSALLAFALILSVGVATPGPTVLLAMSNGSRYGLRHATVGMLGAVTADVVLVALVGFGLGMLLDASETAFVTLKLAGA
AWLAYVGVRMLLSSGGSAAAPSLEHATPDRRAAFLKSFFVAMSNPKYYLFMSALLPQFVDRSHAIAPQYAILAATIVAID
VVGMTGYALLGVHSVRVWKAAGEKWLNRVSGSLLLMLAGYVALYRKAAN
>Mature_208_residues
SLSALLAFALILSVGVATPGPTVLLAMSNGSRYGLRHATVGMLGAVTADVVLVALVGFGLGMLLDASETAFVTLKLAGAA
WLAYVGVRMLLSSGGSAAAPSLEHATPDRRAAFLKSFFVAMSNPKYYLFMSALLPQFVDRSHAIAPQYAILAATIVAIDV
VGMTGYALLGVHSVRVWKAAGEKWLNRVSGSLLLMLAGYVALYRKAAN

Specific function: Conducts the efflux of homoserine and homoserine lactone [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the rht family [H]

Homologues:

Organism=Escherichia coli, GI48994979, Length=209, Percent_Identity=32.5358851674641, Blast_Score=99, Evalue=2e-22,
Organism=Escherichia coli, GI1788099, Length=193, Percent_Identity=27.4611398963731, Blast_Score=63, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004778
- InterPro:   IPR001123 [H]

Pfam domain/function: PF01810 LysE [H]

EC number: NA

Molecular weight: Translated: 21833; Mature: 21702

Theoretical pI: Translated: 10.25; Mature: 10.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLSALLAFALILSVGVATPGPTVLLAMSNGSRYGLRHATVGMLGAVTADVVLVALVGFG
CCHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LGMLLDASETAFVTLKLAGAAWLAYVGVRMLLSSGGSAAAPSLEHATPDRRAAFLKSFFV
HHHEECCCCCEEEEEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHH
AMSNPKYYLFMSALLPQFVDRSHAIAPQYAILAATIVAIDVVGMTGYALLGVHSVRVWKA
HHCCCCCEEHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AGEKWLNRVSGSLLLMLAGYVALYRKAAN
HHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SLSALLAFALILSVGVATPGPTVLLAMSNGSRYGLRHATVGMLGAVTADVVLVALVGFG
CHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LGMLLDASETAFVTLKLAGAAWLAYVGVRMLLSSGGSAAAPSLEHATPDRRAAFLKSFFV
HHHEECCCCCEEEEEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHH
AMSNPKYYLFMSALLPQFVDRSHAIAPQYAILAATIVAIDVVGMTGYALLGVHSVRVWKA
HHCCCCCEEHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AGEKWLNRVSGSLLLMLAGYVALYRKAAN
HHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; homoserine [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + homoserine lactone [Cytoplasm] = Proton [Cytoplasm] + homoserine lactone [Periplasm] Proton [Periplasm] + homoserine [Cytoplasm] = Proton [Cytoplasm] + homoserine [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]