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The map label for this gene is lpdA [H]

Identifier: 78066910

GI number: 78066910

Start: 2509535

End: 2511301

Strand: Reverse

Name: lpdA [H]

Synonym: Bcep18194_A5441

Alternate gene names: 78066910

Gene position: 2511301-2509535 (Counterclockwise)

Preceding gene: 78066911

Following gene: 78066908

Centisome position: 67.98

GC content: 64.57

Gene sequence:

>1767_bases
ATGAGTCTCATCGAAGTCAAGGTTCCGGATATCGGCGATTTCAGCGGCGTCGATGTCATCGAAGTCAACGTCAAACCCGG
CGACGTGATCGAAAAAGAGCAAACGCTCATCACGCTCGAATCCGATAAAGCCTCCATGGAAGTGCCCAGCGACGTCGCCG
GCACCGTCAAGGAAATCAAGGTCAAAGCCGGCGAGAAAGTCTCGCAAGGCACCGTCATCGCCATCGTCGAAGCAGCAGCA
GGCGCCGCCGCACCCGCGAAAGCACCCGAAGCGGCCAAGCCCGCCGCAGCTGCACCCGCACCGGCCGCAGCCGCTCCGGC
TCCCGCACCGCAAGCCGGCAGCTACGCTGGCGCCGTCGACGTCGAATGCGACATGCTCGTGCTCGGCGCCGGCCCCGGTG
GCTACTCGGCCGCGTTCCGCGCTGCCGACCTCGGCATGAAGACCGTGCTCGTCGAACGCTATTCGACGCTCGGCGGCGTG
TGCCTGAACGTCGGCTGCATCCCGTCGAAAGCACTGCTGCATACGTCGCTCGTCGTCGAAGAAGCCGCGGCGCTCGCGTC
GCACGGCATCACGTTCGGCAAGCCGGAAGTCGATCTCGACAAGCTGCGCGACTTCAAGGGCGGCGTCGTCAAGAAGCTGA
CGACCGGCCTTGCCGGCATGGCGAAGGCACGCAAGGTCGAAGTGGTCACGGGCGTCGGCGCATTCGTCGATCCGTTCCAC
ATGGAAGTGCAGGGCGAAAACGGCAAGAAGGTCGTGAAGTTCAAGCAGGCGATCATCGCTGCGGGCTCGCAGGCCGTGAA
GCTGCCGTTCATGCCGGAAGACCCGCGCGTCGTCGATTCGACCGGCGCACTCGAACTGCGTCAACTGCCGAAACGCATGC
TCGTGATCGGCGGCGGCATCATCGGCCTCGAAATGGCCACGGTGTACTCGACGCTCGGCGCCGAGATCGACGTCGTCGAA
ATGATGGACGGCCTGATGATGGGCGCGGACCGCGATCTCGTGAAGGTCTGGGAAAAGTACAACGCGAAGCGCTTCGGCAA
CGTGATGCTGAAGACCAAGACGGTCGGCGCGGAAGCGAAGGAAGACGGCATCTACGTGAAGTTCGAGGGCGAGAAGGCGC
CGGCGGAAGCGCAGCGCTACGACCTCGTGCTCGTCGCGGTGGGCCGCAGCCCGAACGGCAAGAAGATCGGCGCCGACAAG
GCTGGCGTTGCGGTCACGGATCGCGGCTTCATCGACGTCGACAAGCAGATGCGCACGAACGTCCCGCACATCTTCGCGAT
CGGCGACGTCGTCGGCCAGCCGATGCTCGCGCACAAGGCCGTGCATGAAGGCCACGTCGCAGCGGAAGCCGCGCACGGCG
AGAAGGCGTACTTCGACGCCTTGCAGATCCCGTCGGTGGCGTACACCGATCCGGAAGTGGCCTGGGCCGGCAAGACGGAA
GACCAGTGCAAGGCCGAAGGCATCAAGTACGGCAAGGCCGTGTTCCCGTGGGCCGCATCGGGCCGCGCGATCGCGAACGG
TCGCGATGAAGGCTTCACGAAGCTGATCTTCGACGAGGAAACCCATCGCGTGATCGGTGGCGCAATCGTCGGCCTGAACG
CAGGCGACCTGATCAGCGAAGTGTGCCTCGCCGTCGAGATGGGCGCGGACGCGGAAGACATCGGCAAGACGATCCATCCG
CACCCGACGCTCGGCGAATCGGTCGGCATGGCCGCCGAGCTGTATGAAGGCGTCTGTACGGACCTGCCGCCGCAACGCAA
GAAGTAA

Upstream 100 bases:

>100_bases
GGTTCTGCCCAGGTTCGCGCTCTTTGATCAGGCCGTAGTCGCGGGTTTTCTCTGCGGGTTTCTCCCTCTCTGTCTGCAGC
TAATCAAGAAGGGGACAGTA

Downstream 100 bases:

>100_bases
CTCAGCGGGCTCGACCGGCAATCTGCCGGCCACGAAAAAACGCCGCCTCCCGGGCCATCCGGGCGGCGGCGTTTTCTTTT
ATTTGCGGCCGATGCTCAAA

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of pyruvate and 2-oxoglutarate dehydrogenases complexes; Glycine cleavage system L protein [H]

Number of amino acids: Translated: 588; Mature: 587

Protein sequence:

>588_residues
MSLIEVKVPDIGDFSGVDVIEVNVKPGDVIEKEQTLITLESDKASMEVPSDVAGTVKEIKVKAGEKVSQGTVIAIVEAAA
GAAAPAKAPEAAKPAAAAPAPAAAAPAPAPQAGSYAGAVDVECDMLVLGAGPGGYSAAFRAADLGMKTVLVERYSTLGGV
CLNVGCIPSKALLHTSLVVEEAAALASHGITFGKPEVDLDKLRDFKGGVVKKLTTGLAGMAKARKVEVVTGVGAFVDPFH
MEVQGENGKKVVKFKQAIIAAGSQAVKLPFMPEDPRVVDSTGALELRQLPKRMLVIGGGIIGLEMATVYSTLGAEIDVVE
MMDGLMMGADRDLVKVWEKYNAKRFGNVMLKTKTVGAEAKEDGIYVKFEGEKAPAEAQRYDLVLVAVGRSPNGKKIGADK
AGVAVTDRGFIDVDKQMRTNVPHIFAIGDVVGQPMLAHKAVHEGHVAAEAAHGEKAYFDALQIPSVAYTDPEVAWAGKTE
DQCKAEGIKYGKAVFPWAASGRAIANGRDEGFTKLIFDEETHRVIGGAIVGLNAGDLISEVCLAVEMGADAEDIGKTIHP
HPTLGESVGMAAELYEGVCTDLPPQRKK

Sequences:

>Translated_588_residues
MSLIEVKVPDIGDFSGVDVIEVNVKPGDVIEKEQTLITLESDKASMEVPSDVAGTVKEIKVKAGEKVSQGTVIAIVEAAA
GAAAPAKAPEAAKPAAAAPAPAAAAPAPAPQAGSYAGAVDVECDMLVLGAGPGGYSAAFRAADLGMKTVLVERYSTLGGV
CLNVGCIPSKALLHTSLVVEEAAALASHGITFGKPEVDLDKLRDFKGGVVKKLTTGLAGMAKARKVEVVTGVGAFVDPFH
MEVQGENGKKVVKFKQAIIAAGSQAVKLPFMPEDPRVVDSTGALELRQLPKRMLVIGGGIIGLEMATVYSTLGAEIDVVE
MMDGLMMGADRDLVKVWEKYNAKRFGNVMLKTKTVGAEAKEDGIYVKFEGEKAPAEAQRYDLVLVAVGRSPNGKKIGADK
AGVAVTDRGFIDVDKQMRTNVPHIFAIGDVVGQPMLAHKAVHEGHVAAEAAHGEKAYFDALQIPSVAYTDPEVAWAGKTE
DQCKAEGIKYGKAVFPWAASGRAIANGRDEGFTKLIFDEETHRVIGGAIVGLNAGDLISEVCLAVEMGADAEDIGKTIHP
HPTLGESVGMAAELYEGVCTDLPPQRKK
>Mature_587_residues
SLIEVKVPDIGDFSGVDVIEVNVKPGDVIEKEQTLITLESDKASMEVPSDVAGTVKEIKVKAGEKVSQGTVIAIVEAAAG
AAAPAKAPEAAKPAAAAPAPAAAAPAPAPQAGSYAGAVDVECDMLVLGAGPGGYSAAFRAADLGMKTVLVERYSTLGGVC
LNVGCIPSKALLHTSLVVEEAAALASHGITFGKPEVDLDKLRDFKGGVVKKLTTGLAGMAKARKVEVVTGVGAFVDPFHM
EVQGENGKKVVKFKQAIIAAGSQAVKLPFMPEDPRVVDSTGALELRQLPKRMLVIGGGIIGLEMATVYSTLGAEIDVVEM
MDGLMMGADRDLVKVWEKYNAKRFGNVMLKTKTVGAEAKEDGIYVKFEGEKAPAEAQRYDLVLVAVGRSPNGKKIGADKA
GVAVTDRGFIDVDKQMRTNVPHIFAIGDVVGQPMLAHKAVHEGHVAAEAAHGEKAYFDALQIPSVAYTDPEVAWAGKTED
QCKAEGIKYGKAVFPWAASGRAIANGRDEGFTKLIFDEETHRVIGGAIVGLNAGDLISEVCLAVEMGADAEDIGKTIHPH
PTLGESVGMAAELYEGVCTDLPPQRKK

Specific function: Lipoamide dehydrogenase is a component of the glycine cleavage system as well as of the alpha-ketoacid dehydrogenase complexes [H]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=454, Percent_Identity=41.8502202643172, Blast_Score=334, Evalue=1e-91,
Organism=Homo sapiens, GI50301238, Length=466, Percent_Identity=27.8969957081545, Blast_Score=148, Evalue=1e-35,
Organism=Homo sapiens, GI291045266, Length=434, Percent_Identity=26.4976958525346, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI148277071, Length=448, Percent_Identity=24.5535714285714, Blast_Score=117, Evalue=4e-26,
Organism=Homo sapiens, GI33519430, Length=448, Percent_Identity=24.5535714285714, Blast_Score=116, Evalue=5e-26,
Organism=Homo sapiens, GI33519428, Length=448, Percent_Identity=24.5535714285714, Blast_Score=116, Evalue=5e-26,
Organism=Homo sapiens, GI33519426, Length=448, Percent_Identity=24.5535714285714, Blast_Score=116, Evalue=5e-26,
Organism=Homo sapiens, GI148277065, Length=448, Percent_Identity=24.5535714285714, Blast_Score=116, Evalue=6e-26,
Organism=Homo sapiens, GI22035672, Length=455, Percent_Identity=26.8131868131868, Blast_Score=115, Evalue=2e-25,
Organism=Homo sapiens, GI291045268, Length=427, Percent_Identity=25.0585480093677, Blast_Score=109, Evalue=6e-24,
Organism=Escherichia coli, GI1786307, Length=473, Percent_Identity=66.1733615221987, Blast_Score=634, Evalue=0.0,
Organism=Escherichia coli, GI87082354, Length=466, Percent_Identity=29.3991416309013, Blast_Score=177, Evalue=2e-45,
Organism=Escherichia coli, GI87081717, Length=450, Percent_Identity=25.3333333333333, Blast_Score=155, Evalue=1e-38,
Organism=Escherichia coli, GI1789915, Length=444, Percent_Identity=28.1531531531532, Blast_Score=144, Evalue=2e-35,
Organism=Escherichia coli, GI1786305, Length=73, Percent_Identity=60.2739726027397, Blast_Score=79, Evalue=8e-16,
Organism=Caenorhabditis elegans, GI32565766, Length=461, Percent_Identity=42.5162689804772, Blast_Score=345, Evalue=6e-95,
Organism=Caenorhabditis elegans, GI17557007, Length=479, Percent_Identity=26.9311064718163, Blast_Score=127, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI71982272, Length=442, Percent_Identity=26.2443438914027, Blast_Score=113, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI71983419, Length=459, Percent_Identity=26.3616557734205, Blast_Score=112, Evalue=4e-25,
Organism=Caenorhabditis elegans, GI71983429, Length=459, Percent_Identity=26.3616557734205, Blast_Score=112, Evalue=5e-25,
Organism=Saccharomyces cerevisiae, GI6321091, Length=457, Percent_Identity=40.4814004376368, Blast_Score=305, Evalue=2e-83,
Organism=Saccharomyces cerevisiae, GI6325240, Length=470, Percent_Identity=28.2978723404255, Blast_Score=190, Evalue=7e-49,
Organism=Saccharomyces cerevisiae, GI6325166, Length=467, Percent_Identity=27.1948608137045, Blast_Score=140, Evalue=8e-34,
Organism=Drosophila melanogaster, GI21358499, Length=480, Percent_Identity=42.5, Blast_Score=357, Evalue=1e-98,
Organism=Drosophila melanogaster, GI24640551, Length=512, Percent_Identity=28.125, Blast_Score=130, Evalue=3e-30,
Organism=Drosophila melanogaster, GI24640549, Length=480, Percent_Identity=28.3333333333333, Blast_Score=127, Evalue=2e-29,
Organism=Drosophila melanogaster, GI24640553, Length=482, Percent_Identity=28.0082987551867, Blast_Score=126, Evalue=5e-29,
Organism=Drosophila melanogaster, GI17737741, Length=470, Percent_Identity=24.6808510638298, Blast_Score=106, Evalue=6e-23,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 61410; Mature: 61279

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: PS00076 PYRIDINE_REDOX_1 ; PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLIEVKVPDIGDFSGVDVIEVNVKPGDVIEKEQTLITLESDKASMEVPSDVAGTVKEIK
CCEEEEECCCCCCCCCCEEEEEECCCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHH
VKAGEKVSQGTVIAIVEAAAGAAAPAKAPEAAKPAAAAPAPAAAAPAPAPQAGSYAGAVD
ECCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEE
VECDMLVLGAGPGGYSAAFRAADLGMKTVLVERYSTLGGVCLNVGCIPSKALLHTSLVVE
CEEEEEEEECCCCCHHHHHHHHHCCHHHHHHHHHHHHCCEEEEECCCCCHHHHHHHHHHH
EAAALASHGITFGKPEVDLDKLRDFKGGVVKKLTTGLAGMAKARKVEVVTGVGAFVDPFH
HHHHHHHCCCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCEE
MEVQGENGKKVVKFKQAIIAAGSQAVKLPFMPEDPRVVDSTGALELRQLPKRMLVIGGGI
EEEECCCCCCHHHHHHHHHHCCCCEEECCCCCCCCCEECCCCCHHHHHCCCEEEEECCCC
IGLEMATVYSTLGAEIDVVEMMDGLMMGADRDLVKVWEKYNAKRFGNVMLKTKTVGAEAK
HHHHHHHHHHHHCCCEEHHHHHHHHHCCCCHHHHHHHHHHCHHHHCCEEEEEEECCCCCC
EDGIYVKFEGEKAPAEAQRYDLVLVAVGRSPNGKKIGADKAGVAVTDRGFIDVDKQMRTN
CCCEEEEECCCCCCCCHHHEEEEEEEECCCCCCCCCCCCCCCEEEECCCCEECCHHHHCC
VPHIFAIGDVVGQPMLAHKAVHEGHVAAEAAHGEKAYFDALQIPSVAYTDPEVAWAGKTE
CCEEEEEHHHHCCCHHHHHHHHCCCCEEECCCCCHHHHHHHCCCCEEECCCCEEECCCCH
DQCKAEGIKYGKAVFPWAASGRAIANGRDEGFTKLIFDEETHRVIGGAIVGLNAGDLISE
HHHHHCCCCCCCEECCCCCCCCEEECCCCCCCEEEEECCCHHEEECEEEEECCHHHHHHH
VCLAVEMGADAEDIGKTIHPHPTLGESVGMAAELYEGVCTDLPPQRKK
HHHHHHCCCCHHHHCCCCCCCCCCCHHHCHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
SLIEVKVPDIGDFSGVDVIEVNVKPGDVIEKEQTLITLESDKASMEVPSDVAGTVKEIK
CEEEEECCCCCCCCCCEEEEEECCCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHH
VKAGEKVSQGTVIAIVEAAAGAAAPAKAPEAAKPAAAAPAPAAAAPAPAPQAGSYAGAVD
ECCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEE
VECDMLVLGAGPGGYSAAFRAADLGMKTVLVERYSTLGGVCLNVGCIPSKALLHTSLVVE
CEEEEEEEECCCCCHHHHHHHHHCCHHHHHHHHHHHHCCEEEEECCCCCHHHHHHHHHHH
EAAALASHGITFGKPEVDLDKLRDFKGGVVKKLTTGLAGMAKARKVEVVTGVGAFVDPFH
HHHHHHHCCCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCEE
MEVQGENGKKVVKFKQAIIAAGSQAVKLPFMPEDPRVVDSTGALELRQLPKRMLVIGGGI
EEEECCCCCCHHHHHHHHHHCCCCEEECCCCCCCCCEECCCCCHHHHHCCCEEEEECCCC
IGLEMATVYSTLGAEIDVVEMMDGLMMGADRDLVKVWEKYNAKRFGNVMLKTKTVGAEAK
HHHHHHHHHHHHCCCEEHHHHHHHHHCCCCHHHHHHHHHHCHHHHCCEEEEEEECCCCCC
EDGIYVKFEGEKAPAEAQRYDLVLVAVGRSPNGKKIGADKAGVAVTDRGFIDVDKQMRTN
CCCEEEEECCCCCCCCHHHEEEEEEEECCCCCCCCCCCCCCCEEEECCCCEECCHHHHCC
VPHIFAIGDVVGQPMLAHKAVHEGHVAAEAAHGEKAYFDALQIPSVAYTDPEVAWAGKTE
CCEEEEEHHHHCCCHHHHHHHHCCCCEEECCCCCHHHHHHHCCCCEEECCCCEEECCCCH
DQCKAEGIKYGKAVFPWAASGRAIANGRDEGFTKLIFDEETHRVIGGAIVGLNAGDLISE
HHHHHCCCCCCCEECCCCCCCCEEECCCCCCCEEEEECCCHHEEECEEEEECCHHHHHHH
VCLAVEMGADAEDIGKTIHPHPTLGESVGMAAELYEGVCTDLPPQRKK
HHHHHHCCCCHHHHCCCCCCCCCCCHHHCHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]