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The map label for this gene is cls

Identifier: 78066812

GI number: 78066812

Start: 2401542

End: 2402981

Strand: Direct

Name: cls

Synonym: Bcep18194_A5343

Alternate gene names: 78066812

Gene position: 2401542-2402981 (Clockwise)

Preceding gene: 78066811

Following gene: 78066814

Centisome position: 65.01

GC content: 67.92

Gene sequence:

>1440_bases
ATGACCCTCGACTGGCTACATCTCGGCACCCTGATCCTGACCATCCACGTGCTCGGGATCGTCGCGGCGTGCCACGCGAT
CATGAACACGCGCACGTCGCAAGGCGCGATCGCGTGGGCCGTCTCGCTCGCGGCCATGCCGTACCTGACGCTCATTCCGT
ACCTGTTCCTCGGCCGCAGCAAGTTCTCCGGCTACGTCGACGCCCGGCGCCACGAGCTGGAAATGTTGCGCATGCACACA
CCGCGCTCGCCGTGGCTCAGCACCGATGCGACCGACGGGCCGGCCGCCGATGCAATCGGCCAGGCCGCGGTGCTCGCGCT
CACGCGGCTCGGCGGCATGCCGTTCCTCGGCGGCAATTCGGTGCGCACGCTGGTGAACGGCGACGCGACGTTCTCGGCGA
TCCTGGCGGCCATCGACGCCGCGCGCGACTACGTGGTGGTGCAGTTCTTCATCGTGCGCGACGATGCGCTCGGCCGGATG
CTGCGCGACTCGCTGCTCGCGCGTGCGGCAGCCGGCGTGCGCTGCTGCCTGCTGTACGACAGCATCGGCAGCTTCGACCT
GCCGCACAGCTACGTCGACACGTTGCGCCGGGGCGGCGTGGAGGTCCACCCGTTCGCGACCAACCGGAAGTTCGTCAACC
GCTTCCAGCTCAACTTCCGCAACCACCGCAAGATCGTCGTCGTCGACGGCAATCGCGCGTTCGTCGGCGGCCACAACGTC
GGCGTCGAGTATCTCGGCGCGAAGCCGCGCCTGTCGCCGTGGCGCGACACGCACATCGAGATCCGCGGGCCGGTGGTCGC
GAGCATCCAGTACGTGTTCGCCGAAGACTGGCACTGGGCGACGCAGAAGCTGCCGCCGCTCGCGTTGCCGCCGCCCGCGC
AGCCCGGCGACAACATGCATTGCCTCGCGGTGCCGATGGGGCCGGCCGACAAGCAGGAGACGGGCTCGCTGTTCTTCGTC
GAGGCGATCAACGCCGCGCGCGAGCGGGTCTGGATCACCACGCCGTACCTCGTCCCCGACGAAGCCGTGATCTCCGCGTT
GAAGCTCGCCGTGATGCGCGGCGTCGACGTGCGCATCCTGATCCCGAGCCGGCGCGATCACTACGTGGTGTTCGAAGCGT
CGAAGCTGTACGCACGCGACCTGGTCGATGCGGGCGTCAAGGTGTTCCGCTACCGGCCCGGCTTTCTGCACCAGAAGGTC
GTGCTGATCGACCGCATCGCGGCGGCGATCGGCAGCGCGAATCTCGACAACCGCTCGTTCCGCCTCAATTTCGAGATCAT
GGTGCTGACCGTCGACCGCACGTTCGCCGACGAGGTCGAGAAAATGCTCGACGCAGATTTCGCGCAGGCGTACGAGGTCG
ATGCGAGCGAATACCGCCGGTCGCCCGCCTGGCGGCGCATCGCGATGCACGTCGCGCGGCTGTTCGCACCGATCCTCTAA

Upstream 100 bases:

>100_bases
GGCTCGCCCACCGTGCGGTGCACCGTTGCGCCGCACGGTTGGGTAAGATGCGCGTAGGCGGCCGCCCGGCCGCGCGACCT
CAGGAGTCCGTCACGCGCCC

Downstream 100 bases:

>100_bases
CGGCCGGGACGGCGGCGGCGGCCGTGCGATCCGCACCGGCGCCGCGAACGCCGCCGGCCGCCGTCACAGCAGCTTGTCGA
TATCCGCGGCGATTTCGTCC

Product: cardiolipin synthetase

Products: Biphosphatidylglycerol; Glycerol. [C]

Alternate protein names: CL synthase [H]

Number of amino acids: Translated: 479; Mature: 478

Protein sequence:

>479_residues
MTLDWLHLGTLILTIHVLGIVAACHAIMNTRTSQGAIAWAVSLAAMPYLTLIPYLFLGRSKFSGYVDARRHELEMLRMHT
PRSPWLSTDATDGPAADAIGQAAVLALTRLGGMPFLGGNSVRTLVNGDATFSAILAAIDAARDYVVVQFFIVRDDALGRM
LRDSLLARAAAGVRCCLLYDSIGSFDLPHSYVDTLRRGGVEVHPFATNRKFVNRFQLNFRNHRKIVVVDGNRAFVGGHNV
GVEYLGAKPRLSPWRDTHIEIRGPVVASIQYVFAEDWHWATQKLPPLALPPPAQPGDNMHCLAVPMGPADKQETGSLFFV
EAINAARERVWITTPYLVPDEAVISALKLAVMRGVDVRILIPSRRDHYVVFEASKLYARDLVDAGVKVFRYRPGFLHQKV
VLIDRIAAAIGSANLDNRSFRLNFEIMVLTVDRTFADEVEKMLDADFAQAYEVDASEYRRSPAWRRIAMHVARLFAPIL

Sequences:

>Translated_479_residues
MTLDWLHLGTLILTIHVLGIVAACHAIMNTRTSQGAIAWAVSLAAMPYLTLIPYLFLGRSKFSGYVDARRHELEMLRMHT
PRSPWLSTDATDGPAADAIGQAAVLALTRLGGMPFLGGNSVRTLVNGDATFSAILAAIDAARDYVVVQFFIVRDDALGRM
LRDSLLARAAAGVRCCLLYDSIGSFDLPHSYVDTLRRGGVEVHPFATNRKFVNRFQLNFRNHRKIVVVDGNRAFVGGHNV
GVEYLGAKPRLSPWRDTHIEIRGPVVASIQYVFAEDWHWATQKLPPLALPPPAQPGDNMHCLAVPMGPADKQETGSLFFV
EAINAARERVWITTPYLVPDEAVISALKLAVMRGVDVRILIPSRRDHYVVFEASKLYARDLVDAGVKVFRYRPGFLHQKV
VLIDRIAAAIGSANLDNRSFRLNFEIMVLTVDRTFADEVEKMLDADFAQAYEVDASEYRRSPAWRRIAMHVARLFAPIL
>Mature_478_residues
TLDWLHLGTLILTIHVLGIVAACHAIMNTRTSQGAIAWAVSLAAMPYLTLIPYLFLGRSKFSGYVDARRHELEMLRMHTP
RSPWLSTDATDGPAADAIGQAAVLALTRLGGMPFLGGNSVRTLVNGDATFSAILAAIDAARDYVVVQFFIVRDDALGRML
RDSLLARAAAGVRCCLLYDSIGSFDLPHSYVDTLRRGGVEVHPFATNRKFVNRFQLNFRNHRKIVVVDGNRAFVGGHNVG
VEYLGAKPRLSPWRDTHIEIRGPVVASIQYVFAEDWHWATQKLPPLALPPPAQPGDNMHCLAVPMGPADKQETGSLFFVE
AINAARERVWITTPYLVPDEAVISALKLAVMRGVDVRILIPSRRDHYVVFEASKLYARDLVDAGVKVFRYRPGFLHQKVV
LIDRIAAAIGSANLDNRSFRLNFEIMVLTVDRTFADEVEKMLDADFAQAYEVDASEYRRSPAWRRIAMHVARLFAPIL

Specific function: Catalyzes the reversible phosphatidyl group transfer from one phosphatidylglycerol molecule to another to form cardiolipin (CL) (diphosphatidylglycerol) and glycerol [H]

COG id: COG1502

COG function: function code I; Phosphatidylserine/phosphatidylglycerophosphate/cardiolipin synthases and related enzymes

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PLD phosphodiesterase domains [H]

Homologues:

Organism=Escherichia coli, GI1787502, Length=467, Percent_Identity=28.6937901498929, Blast_Score=191, Evalue=1e-49,
Organism=Escherichia coli, GI1787007, Length=351, Percent_Identity=31.3390313390313, Blast_Score=136, Evalue=3e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022924
- InterPro:   IPR001736 [H]

Pfam domain/function: PF00614 PLDc [H]

EC number: 2.7.8.- [C]

Molecular weight: Translated: 53268; Mature: 53137

Theoretical pI: Translated: 9.21; Mature: 9.21

Prosite motif: PS50035 PLD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLDWLHLGTLILTIHVLGIVAACHAIMNTRTSQGAIAWAVSLAAMPYLTLIPYLFLGRS
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
KFSGYVDARRHELEMLRMHTPRSPWLSTDATDGPAADAIGQAAVLALTRLGGMPFLGGNS
CCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEECCCC
VRTLVNGDATFSAILAAIDAARDYVVVQFFIVRDDALGRMLRDSLLARAAAGVRCCLLYD
EEEEECCCHHHHHHHHHHHHCCCEEEEEEEEEECHHHHHHHHHHHHHHHHCCCEEEEEEE
SIGSFDLPHSYVDTLRRGGVEVHPFATNRKFVNRFQLNFRNHRKIVVVDGNRAFVGGHNV
CCCCCCCCHHHHHHHHHCCEEEEEECCCHHHHHHHEECCCCCCEEEEEECCEEEECCCCC
GVEYLGAKPRLSPWRDTHIEIRGPVVASIQYVFAEDWHWATQKLPPLALPPPAQPGDNMH
CEEEECCCCCCCCCCCCEEEEECCCEEEEEEEEECCCCHHHHCCCCCCCCCCCCCCCCEE
CLAVPMGPADKQETGSLFFVEAINAARERVWITTPYLVPDEAVISALKLAVMRGVDVRIL
EEEEECCCCCCCCCCCEEEEEEHHHHHCEEEEECCCCCCCHHHHHHHHHHHHCCCCEEEE
IPSRRDHYVVFEASKLYARDLVDAGVKVFRYRPGFLHQKVVLIDRIAAAIGSANLDNRSF
ECCCCCCEEEEECHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCEE
RLNFEIMVLTVDRTFADEVEKMLDADFAQAYEVDASEYRRSPAWRRIAMHVARLFAPIL
EEEEEEEEEEECCHHHHHHHHHHCCHHHHHHCCCHHHHHCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TLDWLHLGTLILTIHVLGIVAACHAIMNTRTSQGAIAWAVSLAAMPYLTLIPYLFLGRS
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
KFSGYVDARRHELEMLRMHTPRSPWLSTDATDGPAADAIGQAAVLALTRLGGMPFLGGNS
CCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEECCCC
VRTLVNGDATFSAILAAIDAARDYVVVQFFIVRDDALGRMLRDSLLARAAAGVRCCLLYD
EEEEECCCHHHHHHHHHHHHCCCEEEEEEEEEECHHHHHHHHHHHHHHHHCCCEEEEEEE
SIGSFDLPHSYVDTLRRGGVEVHPFATNRKFVNRFQLNFRNHRKIVVVDGNRAFVGGHNV
CCCCCCCCHHHHHHHHHCCEEEEEECCCHHHHHHHEECCCCCCEEEEEECCEEEECCCCC
GVEYLGAKPRLSPWRDTHIEIRGPVVASIQYVFAEDWHWATQKLPPLALPPPAQPGDNMH
CEEEECCCCCCCCCCCCEEEEECCCEEEEEEEEECCCCHHHHCCCCCCCCCCCCCCCCEE
CLAVPMGPADKQETGSLFFVEAINAARERVWITTPYLVPDEAVISALKLAVMRGVDVRIL
EEEEECCCCCCCCCCCEEEEEEHHHHHCEEEEECCCCCCCHHHHHHHHHHHHCCCCEEEE
IPSRRDHYVVFEASKLYARDLVDAGVKVFRYRPGFLHQKVVLIDRIAAAIGSANLDNRSF
ECCCCCCEEEEECHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCEE
RLNFEIMVLTVDRTFADEVEKMLDADFAQAYEVDASEYRRSPAWRRIAMHVARLFAPIL
EEEEEEEEEEECCHHHHHHHHHHCCHHHHHHCCCHHHHHCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Phosphatidylglycerol [C]

Specific reaction: 2 Phosphatidylglycerol = Biphosphatidylglycerol + Glycerol. [C]

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA