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The map label for this gene is dapE [H]

Identifier: 78066810

GI number: 78066810

Start: 2399374

End: 2400513

Strand: Direct

Name: dapE [H]

Synonym: Bcep18194_A5341

Alternate gene names: 78066810

Gene position: 2399374-2400513 (Clockwise)

Preceding gene: 78066809

Following gene: 78066811

Centisome position: 64.95

GC content: 67.81

Gene sequence:

>1140_bases
ATGTCCGCCACCCTAGCCCTTACCGAACAGCTGATCGCCCGCGCGTCCGTGACGCCCGACGACCAGCATTGCCAGCAGAT
CATGACCGAGCGCCTCACCGCGCTCGGCTTCGAATGCGAGACCATCGCGTCGCACGGCGTGACCAACCTGTGGGCCGTCA
AGCGCGGCACCGACGGCCGCGACGGCAAGCTGCTCGCGTTCGCGGGCCACACCGACGTCGTGCCGACCGGCCCGCTCGAG
CAGTGGAGCTCGCCGCCGTTCATCCCCGCCCATCGTGACGGCAAGCTGTACGGCCGCGGCGCGGCCGACATGAAGACATC
GCTCGCGGCATTCGTCGTCGCGTCCGAGGAATTCGTCGCGGCCCATCCCGGCCACCGCGGCACGATCGCGTTCCTGATCA
CGAGCGACGAGGAAGGCCCCGCCACCGACGGCACCGTGAAGGTCGTCGAGCTGCTCGAAGCGCGCGGCGAACGCATGGAC
TACTGCATCGTCGGCGAGCCGACGTCGACCACCGAGCTTGGTGATGTCGTGAAGAACGGCCGCCGCGGCTCGATGTCGGG
CGAACTGGTCGTCAAGGGCGTGCAGGGCCACATCGCGTATCCGCACCTCGCGAAGAACCCGATCCACCTGCTCGCGCCGG
CGCTCGCCGAGCTCGCCGCCGAGCAGTGGGACGAAGGCAACGAATACTTCCCGCCGACCACCTGGCAGGTGTCGAACCTG
CACGCCGGTACCGGTGCGTCCAACGTGATTCCCGGCCACGCCGACCTGCTGTTCAACTTCCGCTTCTCGACCGCCAGCAC
GGTCGAGGGCCTGCAGGCACGCGTGCACGCGATTCTCGACAAGCATGGCCTCGAATACACGCTGAAGTGGTCGGTGAGCG
GCCTGCCGTTCCTCACGCCGCGCGGCGAGCTGTCGGGCGCGCTGGAAAACGCGATCCGCACCGAAACCGGCATCACGACC
GAACTGTCGACGACGGGCGGCACGTCGGACGGCCGCTTCATCGCGCGCATCTGCCCGCAGGTGATCGAGTTCGGCCCGCC
GAACGGCAGCATCCACAAGATCGACGAGCACATCGAAGTGCGCTTCGTCGACCCGCTGAAGAACGTGTACCGCCGCGTGC
TCGAACAACTGATCGCCTGA

Upstream 100 bases:

>100_bases
TGCCGCATAGGGCTGCCCGCGCTGCGCCTGTCGGCGCAGCCCGACGTCTTGTCGCTGCCGGCCACCGCGCCGGCATTTTT
TATCGAAAGTGGTCCGAACC

Downstream 100 bases:

>100_bases
TGGAGCCTCGCATGACGACACCTTTTGCCACTGTTCGCGACCTGCTGCGCTACGCGGTCACGCGCTTCTCGAAGGCGAAG
CTCGCGTTCGGCCACGGCTC

Product: succinyl-diaminopimelate desuccinylase

Products: NA

Alternate protein names: SDAP desuccinylase; N-succinyl-LL-2,6-diaminoheptanedioate amidohydrolase [H]

Number of amino acids: Translated: 379; Mature: 378

Protein sequence:

>379_residues
MSATLALTEQLIARASVTPDDQHCQQIMTERLTALGFECETIASHGVTNLWAVKRGTDGRDGKLLAFAGHTDVVPTGPLE
QWSSPPFIPAHRDGKLYGRGAADMKTSLAAFVVASEEFVAAHPGHRGTIAFLITSDEEGPATDGTVKVVELLEARGERMD
YCIVGEPTSTTELGDVVKNGRRGSMSGELVVKGVQGHIAYPHLAKNPIHLLAPALAELAAEQWDEGNEYFPPTTWQVSNL
HAGTGASNVIPGHADLLFNFRFSTASTVEGLQARVHAILDKHGLEYTLKWSVSGLPFLTPRGELSGALENAIRTETGITT
ELSTTGGTSDGRFIARICPQVIEFGPPNGSIHKIDEHIEVRFVDPLKNVYRRVLEQLIA

Sequences:

>Translated_379_residues
MSATLALTEQLIARASVTPDDQHCQQIMTERLTALGFECETIASHGVTNLWAVKRGTDGRDGKLLAFAGHTDVVPTGPLE
QWSSPPFIPAHRDGKLYGRGAADMKTSLAAFVVASEEFVAAHPGHRGTIAFLITSDEEGPATDGTVKVVELLEARGERMD
YCIVGEPTSTTELGDVVKNGRRGSMSGELVVKGVQGHIAYPHLAKNPIHLLAPALAELAAEQWDEGNEYFPPTTWQVSNL
HAGTGASNVIPGHADLLFNFRFSTASTVEGLQARVHAILDKHGLEYTLKWSVSGLPFLTPRGELSGALENAIRTETGITT
ELSTTGGTSDGRFIARICPQVIEFGPPNGSIHKIDEHIEVRFVDPLKNVYRRVLEQLIA
>Mature_378_residues
SATLALTEQLIARASVTPDDQHCQQIMTERLTALGFECETIASHGVTNLWAVKRGTDGRDGKLLAFAGHTDVVPTGPLEQ
WSSPPFIPAHRDGKLYGRGAADMKTSLAAFVVASEEFVAAHPGHRGTIAFLITSDEEGPATDGTVKVVELLEARGERMDY
CIVGEPTSTTELGDVVKNGRRGSMSGELVVKGVQGHIAYPHLAKNPIHLLAPALAELAAEQWDEGNEYFPPTTWQVSNLH
AGTGASNVIPGHADLLFNFRFSTASTVEGLQARVHAILDKHGLEYTLKWSVSGLPFLTPRGELSGALENAIRTETGITTE
LSTTGGTSDGRFIARICPQVIEFGPPNGSIHKIDEHIEVRFVDPLKNVYRRVLEQLIA

Specific function: Catalyzes the hydrolysis of N-succinyl-L,L- diaminopimelic acid (SDAP), forming succinate and LL-2,6- diaminoheptanedioate (DAP), an intermediate involved in the bacterial biosynthesis of lysine and meso-diaminopimelic acid, an essential component of bact

COG id: COG0624

COG function: function code E; Acetylornithine deacetylase/Succinyl-diaminopimelate desuccinylase and related deacylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20A family. DapE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788816, Length=376, Percent_Identity=56.9148936170213, Blast_Score=433, Evalue=1e-122,
Organism=Escherichia coli, GI1790395, Length=391, Percent_Identity=25.5754475703325, Blast_Score=89, Evalue=6e-19,
Organism=Escherichia coli, GI1789236, Length=269, Percent_Identity=23.4200743494424, Blast_Score=66, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001261
- InterPro:   IPR005941
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: =3.5.1.18 [H]

Molecular weight: Translated: 40886; Mature: 40754

Theoretical pI: Translated: 5.69; Mature: 5.69

Prosite motif: PS00758 ARGE_DAPE_CPG2_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSATLALTEQLIARASVTPDDQHCQQIMTERLTALGFECETIASHGVTNLWAVKRGTDGR
CCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHCCCCEEEEEECCCCCC
DGKLLAFAGHTDVVPTGPLEQWSSPPFIPAHRDGKLYGRGAADMKTSLAAFVVASEEFVA
CCCEEEEECCCCCCCCCCHHHCCCCCCCCCCCCCCEEECCCHHHHHHHHHEEEECCHHEE
AHPGHRGTIAFLITSDEEGPATDGTVKVVELLEARGERMDYCIVGEPTSTTELGDVVKNG
ECCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHCC
RRGSMSGELVVKGVQGHIAYPHLAKNPIHLLAPALAELAAEQWDEGNEYFPPTTWQVSNL
CCCCCCCCEEEEECCCCEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEECCE
HAGTGASNVIPGHADLLFNFRFSTASTVEGLQARVHAILDKHGLEYTLKWSVSGLPFLTP
ECCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCEECC
RGELSGALENAIRTETGITTELSTTGGTSDGRFIARICPQVIEFGPPNGSIHKIDEHIEV
CHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHCCCCCCCEEEECCCEEE
RFVDPLKNVYRRVLEQLIA
EEECHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SATLALTEQLIARASVTPDDQHCQQIMTERLTALGFECETIASHGVTNLWAVKRGTDGR
CCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHCCCCEEEEEECCCCCC
DGKLLAFAGHTDVVPTGPLEQWSSPPFIPAHRDGKLYGRGAADMKTSLAAFVVASEEFVA
CCCEEEEECCCCCCCCCCHHHCCCCCCCCCCCCCCEEECCCHHHHHHHHHEEEECCHHEE
AHPGHRGTIAFLITSDEEGPATDGTVKVVELLEARGERMDYCIVGEPTSTTELGDVVKNG
ECCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHCC
RRGSMSGELVVKGVQGHIAYPHLAKNPIHLLAPALAELAAEQWDEGNEYFPPTTWQVSNL
CCCCCCCCEEEEECCCCEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEECCE
HAGTGASNVIPGHADLLFNFRFSTASTVEGLQARVHAILDKHGLEYTLKWSVSGLPFLTP
ECCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCEECC
RGELSGALENAIRTETGITTELSTTGGTSDGRFIARICPQVIEFGPPNGSIHKIDEHIEV
CHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHCCCCCCCEEEECCCEEE
RFVDPLKNVYRRVLEQLIA
EEECHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA