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The map label for this gene is yjfH [C]

Identifier: 78066783

GI number: 78066783

Start: 2366501

End: 2367283

Strand: Reverse

Name: yjfH [C]

Synonym: Bcep18194_A5314

Alternate gene names: 78066783

Gene position: 2367283-2366501 (Counterclockwise)

Preceding gene: 78066784

Following gene: 78066781

Centisome position: 64.08

GC content: 70.5

Gene sequence:

>783_bases
ATGAAAAGCATTACTTCCCGCGACAACCCGCTGTACAAGCGCCTGAAGGCGCTCGCGGGTTCGACGTCCCATCAGCGCCG
CGGCGGCCAGGCGCTGCTCGAAGGATTCCATCTCGCAAGCGCGTACCTCGACACGGGTGCGACGCCCGAGCTGTGCGTCA
CGACCGAAGGTGCACTCGGCCACGCCGAGGCCCAGGCGATCGTCGCGCGCGTCGATGCGCAACGGATCGTCACGCTGCCC
GACGCGCTGTTCGGGCAGTTGTCGAACGTCGTCAACGGCGTCGGCTTCCTGCTGCTCGTCGACCGGCCGGTACAGCCGCT
GCCGGCGCGCGTGACGCAGTCGTCGGTCGTGCTCGACGGCGTGCAGGACGCCGGCAACGTCGGCTCGATCCTGCGCAGCG
CGGCGGCCGCAGGCGTTCGCCACGTGTTCTGTGCGCCGGGTACCGCGTACGCATGGTCGTCGAAGGTGCTGCGCTCGGGG
ATGGGGGCGCATTTCCTGCTGTCGATCCACGAGGACGTCGAAGCCGGCGCGCTCGCCGAGCGCCTCGATGTGCCGGTCGC
GCTGACCGACTCGCACGGCGCGCAGGCGGTCTATGACTGCGACCTGTCGGGGCCGGTTGCCTGGGTGTTCGGCAACGAGG
GGGCCGGCGTGTCGGCGTTCTGGCGCGACGCGGCCACGCATCGCGTGACGATCCCGCAGCCGGGCGGGATGGAGTCGCTG
AACGTCGCGGCCGCGGCTGCGGTCTGCCTGTTCGAGCAGGTGCGTCAGCAGCGGCGTGCGTGA

Upstream 100 bases:

>100_bases
ACGGCACCGCGAAACACCTTGCCGCACTGCGCGAGCACGGCCCGTGCGAAGCCCATCGGCGCTCGTTCGCGCCGGTTCGT
GCGGCACTCGACCTGATTCG

Downstream 100 bases:

>100_bases
CGCCGCACGCGTCTTGCTCCGCACCATGAAAAAGGCCGCGTTCGACGCGGCCTTTTTGTTTCCGGCGGCGGCCCTGCGGC
CGCCAGCGCCCAGTCTTAGT

Product: tRNA/rRNA methyltransferase (SpoU)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 260; Mature: 260

Protein sequence:

>260_residues
MKSITSRDNPLYKRLKALAGSTSHQRRGGQALLEGFHLASAYLDTGATPELCVTTEGALGHAEAQAIVARVDAQRIVTLP
DALFGQLSNVVNGVGFLLLVDRPVQPLPARVTQSSVVLDGVQDAGNVGSILRSAAAAGVRHVFCAPGTAYAWSSKVLRSG
MGAHFLLSIHEDVEAGALAERLDVPVALTDSHGAQAVYDCDLSGPVAWVFGNEGAGVSAFWRDAATHRVTIPQPGGMESL
NVAAAAAVCLFEQVRQQRRA

Sequences:

>Translated_260_residues
MKSITSRDNPLYKRLKALAGSTSHQRRGGQALLEGFHLASAYLDTGATPELCVTTEGALGHAEAQAIVARVDAQRIVTLP
DALFGQLSNVVNGVGFLLLVDRPVQPLPARVTQSSVVLDGVQDAGNVGSILRSAAAAGVRHVFCAPGTAYAWSSKVLRSG
MGAHFLLSIHEDVEAGALAERLDVPVALTDSHGAQAVYDCDLSGPVAWVFGNEGAGVSAFWRDAATHRVTIPQPGGMESL
NVAAAAAVCLFEQVRQQRRA
>Mature_260_residues
MKSITSRDNPLYKRLKALAGSTSHQRRGGQALLEGFHLASAYLDTGATPELCVTTEGALGHAEAQAIVARVDAQRIVTLP
DALFGQLSNVVNGVGFLLLVDRPVQPLPARVTQSSVVLDGVQDAGNVGSILRSAAAAGVRHVFCAPGTAYAWSSKVLRSG
MGAHFLLSIHEDVEAGALAERLDVPVALTDSHGAQAVYDCDLSGPVAWVFGNEGAGVSAFWRDAATHRVTIPQPGGMESL
NVAAAAAVCLFEQVRQQRRA

Specific function: Unknown

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Homo sapiens, GI8922534, Length=304, Percent_Identity=29.6052631578947, Blast_Score=84, Evalue=1e-16,
Organism=Escherichia coli, GI1790623, Length=145, Percent_Identity=30.3448275862069, Blast_Score=73, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24666840, Length=287, Percent_Identity=27.5261324041812, Blast_Score=78, Evalue=7e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001537
- InterPro:   IPR013123 [H]

Pfam domain/function: PF00588 SpoU_methylase; PF08032 SpoU_sub_bind [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 27244; Mature: 27244

Theoretical pI: Translated: 6.95; Mature: 6.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSITSRDNPLYKRLKALAGSTSHQRRGGQALLEGFHLASAYLDTGATPELCVTTEGALG
CCCCCCCCCHHHHHHHHHHCCCHHHHCCCHHHHHHHHHHHHHHCCCCCCCEEEEECCCCC
HAEAQAIVARVDAQRIVTLPDALFGQLSNVVNGVGFLLLVDRPVQPLPARVTQSSVVLDG
HHHHHHHHHHHCHHHEEECCHHHHHHHHHHHHHCEEEEEECCCCCCCCCHHCCCHHEEEC
VQDAGNVGSILRSAAAAGVRHVFCAPGTAYAWSSKVLRSGMGAHFLLSIHEDVEAGALAE
CCCCCHHHHHHHHHHHHHHHEEEECCCCCHHHHHHHHHCCCCCEEEEEECCCHHHHHHHH
RLDVPVALTDSHGAQAVYDCDLSGPVAWVFGNEGAGVSAFWRDAATHRVTIPQPGGMESL
HHCCCEEEECCCCCCEEEEECCCCCEEEEECCCCCCHHHHHHCCCCCEEECCCCCCCCHH
NVAAAAAVCLFEQVRQQRRA
HHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKSITSRDNPLYKRLKALAGSTSHQRRGGQALLEGFHLASAYLDTGATPELCVTTEGALG
CCCCCCCCCHHHHHHHHHHCCCHHHHCCCHHHHHHHHHHHHHHCCCCCCCEEEEECCCCC
HAEAQAIVARVDAQRIVTLPDALFGQLSNVVNGVGFLLLVDRPVQPLPARVTQSSVVLDG
HHHHHHHHHHHCHHHEEECCHHHHHHHHHHHHHCEEEEEECCCCCCCCCHHCCCHHEEEC
VQDAGNVGSILRSAAAAGVRHVFCAPGTAYAWSSKVLRSGMGAHFLLSIHEDVEAGALAE
CCCCCHHHHHHHHHHHHHHHEEEECCCCCHHHHHHHHHCCCCCEEEEEECCCHHHHHHHH
RLDVPVALTDSHGAQAVYDCDLSGPVAWVFGNEGAGVSAFWRDAATHRVTIPQPGGMESL
HHCCCEEEECCCCCCEEEEECCCCCEEEEECCCCCCHHHHHHCCCCCEEECCCCCCCCHH
NVAAAAAVCLFEQVRQQRRA
HHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]