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The map label for this gene is tesA [H]

Identifier: 78066689

GI number: 78066689

Start: 2272076

End: 2272750

Strand: Direct

Name: tesA [H]

Synonym: Bcep18194_A5220

Alternate gene names: 78066689

Gene position: 2272076-2272750 (Clockwise)

Preceding gene: 78066687

Following gene: 78066695

Centisome position: 61.51

GC content: 68.89

Gene sequence:

>675_bases
ATGGACACGACATTTCGCTGGAAAAGACGCGCGGCGTTCGCCGCGCTGCTGGGCACCCTGCTGGCCGCCACCGTATCGGC
ACGCGCTGCGACGACGCCGGCCGCGACATCGGGCCAGCCCGTGATCGTCGTGCTCGGCGACAGCCTGTCGGCCGAATACG
GGTTGCCGCGCGACACCGGCTGGGTGGCATTGCTGCGGCAACGGCTCACGACCGAGCGAATCGATTATAGCGTCGCGAAC
GCCAGCGTCAGCGGCGACACCACGAGCGGCGGCCGAGCGCGGCTGCCCGCGGTGCTGCAACGGCTCAAGCCGTCGATCGT
CGTCGTCGAGCTCGGGTCGAACGATGCGCTGCGCGGCGTGCCGCTCGCCACGACCGAGCAGAACCTGCGCGACATCATCG
CCGGCGCGCGGCAGGCGCGGGCGAAGGTCGTGCTGGTCGGCATGTACGTGCCACCCAATTACGGCCCCGACTACACGCAG
AAGTTCCACGCCGTCTATACGCGGCTGTCGAAGGATCTCGGCGTGCCGCTCGTGCCGTTCCTGCTGGCCGGCATCGAGAA
CAAGCCGGAGTTGTTCCAGGCCGACCAGATGCATCCTGCGCAGCAGGCGCAGGGCATTCTGCTCGACAACGTCTGGCCGG
CGCTGAAACCGCTGCTCGGCAAGCCGCGCGGCTGA

Upstream 100 bases:

>100_bases
GGCGAGCGTGATGCCGTCGAGGATCGTCAGCTCGCCCGTTGCATCGGCGACCCGCTTGCACACGTCATGGACTTCGATGA
TCGGATCGGTAATCTTGAAC

Downstream 100 bases:

>100_bases
CGCCACCGCCCCGCCCGGAAAACAAAAAGCCCCGCATGAAGCGGGGCTTTTTTTGTTGCGTGCGAAACGACGCTCAGTTG
TCGCCGTCCTGCGACTGGCC

Product: lipolytic protein

Products: NA

Alternate protein names: Lecithinase B; Lysophospholipase L1; Protease I [H]

Number of amino acids: Translated: 224; Mature: 224

Protein sequence:

>224_residues
MDTTFRWKRRAAFAALLGTLLAATVSARAATTPAATSGQPVIVVLGDSLSAEYGLPRDTGWVALLRQRLTTERIDYSVAN
ASVSGDTTSGGRARLPAVLQRLKPSIVVVELGSNDALRGVPLATTEQNLRDIIAGARQARAKVVLVGMYVPPNYGPDYTQ
KFHAVYTRLSKDLGVPLVPFLLAGIENKPELFQADQMHPAQQAQGILLDNVWPALKPLLGKPRG

Sequences:

>Translated_224_residues
MDTTFRWKRRAAFAALLGTLLAATVSARAATTPAATSGQPVIVVLGDSLSAEYGLPRDTGWVALLRQRLTTERIDYSVAN
ASVSGDTTSGGRARLPAVLQRLKPSIVVVELGSNDALRGVPLATTEQNLRDIIAGARQARAKVVLVGMYVPPNYGPDYTQ
KFHAVYTRLSKDLGVPLVPFLLAGIENKPELFQADQMHPAQQAQGILLDNVWPALKPLLGKPRG
>Mature_224_residues
MDTTFRWKRRAAFAALLGTLLAATVSARAATTPAATSGQPVIVVLGDSLSAEYGLPRDTGWVALLRQRLTTERIDYSVAN
ASVSGDTTSGGRARLPAVLQRLKPSIVVVELGSNDALRGVPLATTEQNLRDIIAGARQARAKVVLVGMYVPPNYGPDYTQ
KFHAVYTRLSKDLGVPLVPFLLAGIENKPELFQADQMHPAQQAQGILLDNVWPALKPLLGKPRG

Specific function: Hydrolyzes only long chain acyl thioesters (C12-C18). Specificity similar to chymotrypsin [H]

COG id: COG2755

COG function: function code E; Lysophospholipase L1 and related esterases

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDSL' lipolytic enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1786702, Length=221, Percent_Identity=38.9140271493213, Blast_Score=147, Evalue=4e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013830
- InterPro:   IPR013831
- InterPro:   IPR001087
- InterPro:   IPR008265 [H]

Pfam domain/function: PF00657 Lipase_GDSL [H]

EC number: =3.1.1.5 [H]

Molecular weight: Translated: 24049; Mature: 24049

Theoretical pI: Translated: 10.48; Mature: 10.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDTTFRWKRRAAFAALLGTLLAATVSARAATTPAATSGQPVIVVLGDSLSAEYGLPRDTG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCCCCCCCHH
WVALLRQRLTTERIDYSVANASVSGDTTSGGRARLPAVLQRLKPSIVVVELGSNDALRGV
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEEECCCCCCCCC
PLATTEQNLRDIIAGARQARAKVVLVGMYVPPNYGPDYTQKFHAVYTRLSKDLGVPLVPF
CCCCCHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCHHHH
LLAGIENKPELFQADQMHPAQQAQGILLDNVWPALKPLLGKPRG
HHHCCCCCCHHHCCCCCCHHHHHCCCCHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MDTTFRWKRRAAFAALLGTLLAATVSARAATTPAATSGQPVIVVLGDSLSAEYGLPRDTG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCCCCCCCHH
WVALLRQRLTTERIDYSVANASVSGDTTSGGRARLPAVLQRLKPSIVVVELGSNDALRGV
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEEECCCCCCCCC
PLATTEQNLRDIIAGARQARAKVVLVGMYVPPNYGPDYTQKFHAVYTRLSKDLGVPLVPF
CCCCCHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCHHHH
LLAGIENKPELFQADQMHPAQQAQGILLDNVWPALKPLLGKPRG
HHHCCCCCCHHHCCCCCCHHHHHCCCCHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]