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The map label for this gene is alkB [H]

Identifier: 78066397

GI number: 78066397

Start: 1947238

End: 1947882

Strand: Direct

Name: alkB [H]

Synonym: Bcep18194_A4927

Alternate gene names: 78066397

Gene position: 1947238-1947882 (Clockwise)

Preceding gene: 78066396

Following gene: 78066398

Centisome position: 52.71

GC content: 59.38

Gene sequence:

>645_bases
ATGGATTCGCTGCATCTTTTCGAGACGGAAGGTGAACACCTCCGCATTGGCGCCGACGCCGTCGTGCTTCGTGGGTTCGC
CCTGCCGTATCTGCACGGACTGTTGCGTGCAATTGTCGATATTGACGCGATCGCTCCGTTTCGGCACATGGTGACGCCTG
GTGGTTTCACCATGTCTGTTGCCTTGACGAACTGCGGGGCATTGGGATGGACGACTGATCGTCGCGGCTATCGCTACACC
TCTGTTGACCCCGACACTGGATTGCCGTGGCCGGCCATGCCGGAGGTGTTTGCACGGCTAGCGAAAGAGGCCGCCGCGGC
AGCAGGGTTCGACGATTTCGAGCCGGATGCCTGTCTCGTCAATCGCTATGCTCCCGGCGCGCGCCTTTCGTTGCATCAGG
ACAAGAACGAGCAGGATTTTAATGCGCCCATCGTCTCGGTCTCTCTGGGCATGAAAGCCGTGTTTCTTTTCGGTGGTCAC
GAGCGCTCCGATCCCACAACCAAAGTGCCGCTTTATCACGGTGACGTGGCCGTGTGGGGAGGCGTTGATCGTCTGCGCTA
CCACGGTGTGATGCCGCTCAAGGAGGATCCGCATGGTCTTCTCGGACGCCAGCGAATCAACTTCACGTTCCGTAAGGCGG
GATGA

Upstream 100 bases:

>100_bases
AGCGCGGACTTCGAAGCGTGGCAATGCGACATGGCGTCAGTCGTATTCGTAGCGGCAGCCGTCACACCGTCGGCCTGATC
TTTCACGATTCGAGGTGAGC

Downstream 100 bases:

>100_bases
CGTCGTGAATTCGACAGCAAGGGACGGAGTGTTTTCTGGTGATGGCGCAACTAAAGTATGGCGGGTTGCTGGAGTGTACC
TATGAAAACCATTTCCGTAG

Product: DNA-N1-methyladenine dioxygenase

Products: NA

Alternate protein names: Alkylated DNA repair protein AlkB [H]

Number of amino acids: Translated: 214; Mature: 214

Protein sequence:

>214_residues
MDSLHLFETEGEHLRIGADAVVLRGFALPYLHGLLRAIVDIDAIAPFRHMVTPGGFTMSVALTNCGALGWTTDRRGYRYT
SVDPDTGLPWPAMPEVFARLAKEAAAAAGFDDFEPDACLVNRYAPGARLSLHQDKNEQDFNAPIVSVSLGMKAVFLFGGH
ERSDPTTKVPLYHGDVAVWGGVDRLRYHGVMPLKEDPHGLLGRQRINFTFRKAG

Sequences:

>Translated_214_residues
MDSLHLFETEGEHLRIGADAVVLRGFALPYLHGLLRAIVDIDAIAPFRHMVTPGGFTMSVALTNCGALGWTTDRRGYRYT
SVDPDTGLPWPAMPEVFARLAKEAAAAAGFDDFEPDACLVNRYAPGARLSLHQDKNEQDFNAPIVSVSLGMKAVFLFGGH
ERSDPTTKVPLYHGDVAVWGGVDRLRYHGVMPLKEDPHGLLGRQRINFTFRKAG
>Mature_214_residues
MDSLHLFETEGEHLRIGADAVVLRGFALPYLHGLLRAIVDIDAIAPFRHMVTPGGFTMSVALTNCGALGWTTDRRGYRYT
SVDPDTGLPWPAMPEVFARLAKEAAAAAGFDDFEPDACLVNRYAPGARLSLHQDKNEQDFNAPIVSVSLGMKAVFLFGGH
ERSDPTTKVPLYHGDVAVWGGVDRLRYHGVMPLKEDPHGLLGRQRINFTFRKAG

Specific function: Dioxygenase that repairs alkylated DNA and RNA containing 3-methylcytosine or 1-methyladenine by oxidative demethylation. Has highest activity towards 3-methylcytosine. Has lower activity towards alkylated DNA containing ethenoadenine, and no detectable a

COG id: COG3145

COG function: function code L; Alkylated DNA repair protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Fe2OG dioxygenase domain [H]

Homologues:

Organism=Homo sapiens, GI87298840, Length=107, Percent_Identity=34.5794392523364, Blast_Score=75, Evalue=4e-14,
Organism=Escherichia coli, GI1788541, Length=199, Percent_Identity=60.3015075376884, Blast_Score=251, Evalue=2e-68,
Organism=Drosophila melanogaster, GI45555401, Length=125, Percent_Identity=36.8, Blast_Score=78, Evalue=5e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004574 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 23502; Mature: 23502

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDSLHLFETEGEHLRIGADAVVLRGFALPYLHGLLRAIVDIDAIAPFRHMVTPGGFTMSV
CCCCEEEECCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCEEEEE
ALTNCGALGWTTDRRGYRYTSVDPDTGLPWPAMPEVFARLAKEAAAAAGFDDFEPDACLV
EEECCCCCCCCCCCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEE
NRYAPGARLSLHQDKNEQDFNAPIVSVSLGMKAVFLFGGHERSDPTTKVPLYHGDVAVWG
ECCCCCCEEEECCCCCCCCCCCCEEEEECCEEEEEEECCCCCCCCCEECEEEECCEEEEC
GVDRLRYHGVMPLKEDPHGLLGRQRINFTFRKAG
CHHHHHHCCCCCCCCCCCCCCCCEEEEEEEEECC
>Mature Secondary Structure
MDSLHLFETEGEHLRIGADAVVLRGFALPYLHGLLRAIVDIDAIAPFRHMVTPGGFTMSV
CCCCEEEECCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCEEEEE
ALTNCGALGWTTDRRGYRYTSVDPDTGLPWPAMPEVFARLAKEAAAAAGFDDFEPDACLV
EEECCCCCCCCCCCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEE
NRYAPGARLSLHQDKNEQDFNAPIVSVSLGMKAVFLFGGHERSDPTTKVPLYHGDVAVWG
ECCCCCCEEEECCCCCCCCCCCCEEEEECCEEEEEEECCCCCCCCCEECEEEECCEEEEC
GVDRLRYHGVMPLKEDPHGLLGRQRINFTFRKAG
CHHHHHHCCCCCCCCCCCCCCCCEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3536913; 9097040; 9278503; 3887409; 7928996 [H]