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The map label for this gene is ada [H]

Identifier: 78066395

GI number: 78066395

Start: 1945956

End: 1946516

Strand: Direct

Name: ada [H]

Synonym: Bcep18194_A4925

Alternate gene names: 78066395

Gene position: 1945956-1946516 (Clockwise)

Preceding gene: 78066394

Following gene: 78066396

Centisome position: 52.68

GC content: 59.71

Gene sequence:

>561_bases
ATGACAGTCCGTTCCGTTTCTACGTTCAAATACGTCTTCCGTGCCCCGGGCAGTCGGGTTGTCGATTCCATTCGATTCGC
CGTCGGTCAAACGACGCTTGGCTTGGTCATGGTCGGACGTAGTCAACGCGGCGTGTGCGCGATCTTTTTGGGTGACGATG
CAATGGCCCTGCAAGCGCAACTCGCAGCAGCGTTCCCGACCAACGAACTGCGCGTTGACCAGGCTGCGCTTGCCTTGGAG
CTCAGACAGGTCATTGCGTTCATCGACAACGAGCAGCCCGCCGGCGTCATCGATCTCGATATTTGTGGGACTGCCTTCCA
GCAAAAGGTTTGGCAGGCGCTGTGTGAAATACCGGCTGGCCAAACCCGGAGCTACAGCGACGTAGCGCGAGATCTCGGTA
CCCCGGAAGCCGTTCGGGCTGTAGCAGGCGCTTGCGCCTCAAATGTCATGGCTATTGCCATTCCTTGTCATCGCGTCGTA
CGCAGCGACGGCTCCGTGTCCGGCTATCGCTGGGGTGTCGAGCGCAAGCTCGCGCTGTTGGCCGAGGAGTGTGCGCAATG
A

Upstream 100 bases:

>100_bases
GTGCTTTCGAGTTCAAGAATTCAACCGCAAATACCGGAGTTTCGATCGACGGGCAAGTTCGTACACTGACATCATCCCGT
AATCAAGTGAGCGATGTCCC

Downstream 100 bases:

>100_bases
AGCTCAAGACTCGGCGTGAGCAGGATATGACGCCCTTGACTTACGATTGGCCGGGTGTCGAACGATCGCTCGATACTTAC
GGCAACGCTGTTCTTCCGAA

Product: DNA-O6-methylguanine--protein-cysteine S-methyltransferase

Products: NA

Alternate protein names: Regulatory protein of adaptative response; O-6-methylguanine-DNA alkyltransferase [H]

Number of amino acids: Translated: 186; Mature: 185

Protein sequence:

>186_residues
MTVRSVSTFKYVFRAPGSRVVDSIRFAVGQTTLGLVMVGRSQRGVCAIFLGDDAMALQAQLAAAFPTNELRVDQAALALE
LRQVIAFIDNEQPAGVIDLDICGTAFQQKVWQALCEIPAGQTRSYSDVARDLGTPEAVRAVAGACASNVMAIAIPCHRVV
RSDGSVSGYRWGVERKLALLAEECAQ

Sequences:

>Translated_186_residues
MTVRSVSTFKYVFRAPGSRVVDSIRFAVGQTTLGLVMVGRSQRGVCAIFLGDDAMALQAQLAAAFPTNELRVDQAALALE
LRQVIAFIDNEQPAGVIDLDICGTAFQQKVWQALCEIPAGQTRSYSDVARDLGTPEAVRAVAGACASNVMAIAIPCHRVV
RSDGSVSGYRWGVERKLALLAEECAQ
>Mature_185_residues
TVRSVSTFKYVFRAPGSRVVDSIRFAVGQTTLGLVMVGRSQRGVCAIFLGDDAMALQAQLAAAFPTNELRVDQAALALEL
RQVIAFIDNEQPAGVIDLDICGTAFQQKVWQALCEIPAGQTRSYSDVARDLGTPEAVRAVAGACASNVMAIAIPCHRVVR
SDGSVSGYRWGVERKLALLAEECAQ

Specific function: The methylated ADA protein acts as a positive regulator of its own synthesis, as well as that of other proteins. The transcription-activating function of the ADA protein resides in its N-terminus. It activates the transcription of alkA, alkB and aidB [H]

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH araC/xylS-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788542, Length=171, Percent_Identity=48.5380116959064, Blast_Score=160, Evalue=5e-41,
Organism=Escherichia coli, GI1787596, Length=81, Percent_Identity=48.1481481481481, Blast_Score=84, Evalue=8e-18,
Organism=Caenorhabditis elegans, GI115533070, Length=83, Percent_Identity=56.6265060240964, Blast_Score=106, Evalue=7e-24,
Organism=Caenorhabditis elegans, GI115533068, Length=83, Percent_Identity=56.6265060240964, Blast_Score=105, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6320001, Length=162, Percent_Identity=32.0987654320988, Blast_Score=92, Evalue=7e-20,
Organism=Drosophila melanogaster, GI17137554, Length=174, Percent_Identity=35.0574712643678, Blast_Score=82, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004026
- InterPro:   IPR016221
- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR018062
- InterPro:   IPR018060
- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF02805 Ada_Zn_binding; PF01035 DNA_binding_1; PF00165 HTH_AraC; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 19918; Mature: 19787

Theoretical pI: Translated: 7.05; Mature: 7.05

Prosite motif: PS00374 MGMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
3.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVRSVSTFKYVFRAPGSRVVDSIRFAVGQTTLGLVMVGRSQRGVCAIFLGDDAMALQAQ
CCCCCHHHHHHHHHCCCHHHHHHHHHHHCCHHEEEEEECCCCCCEEEEEECCCHHHHHHH
LAAAFPTNELRVDQAALALELRQVIAFIDNEQPAGVIDLDICGTAFQQKVWQALCEIPAG
HHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEHHHCHHHHHHHHHHHHCCCCC
QTRSYSDVARDLGTPEAVRAVAGACASNVMAIAIPCHRVVRSDGSVSGYRWGVERKLALL
CCCCHHHHHHHCCCHHHHHHHHHHHHHCCEEEEEHHHHHHHCCCCCCCEECCHHHHHHHH
AEECAQ
HHHHCC
>Mature Secondary Structure 
TVRSVSTFKYVFRAPGSRVVDSIRFAVGQTTLGLVMVGRSQRGVCAIFLGDDAMALQAQ
CCCCHHHHHHHHHCCCHHHHHHHHHHHCCHHEEEEEECCCCCCEEEEEECCCHHHHHHH
LAAAFPTNELRVDQAALALELRQVIAFIDNEQPAGVIDLDICGTAFQQKVWQALCEIPAG
HHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEHHHCHHHHHHHHHHHHCCCCC
QTRSYSDVARDLGTPEAVRAVAGACASNVMAIAIPCHRVVRSDGSVSGYRWGVERKLALL
CCCCHHHHHHHCCCHHHHHHHHHHHHHCCEEEEEHHHHHHHCCCCCCCEECCHHHHHHHH
AEECAQ
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1904855; 11677609 [H]