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The map label for this gene is alkB [H]

Identifier: 78066391

GI number: 78066391

Start: 1942448

End: 1943098

Strand: Direct

Name: alkB [H]

Synonym: Bcep18194_A4921

Alternate gene names: 78066391

Gene position: 1942448-1943098 (Clockwise)

Preceding gene: 78066390

Following gene: 78066393

Centisome position: 52.58

GC content: 58.37

Gene sequence:

>651_bases
ATGAGCACGTTCGACAAGGACCGCTACTGGTCTGTGGATGGGCGCGAAATCCTCGGGGAGAACGCATTCATTCTCCGAGG
ATTTGCGCTGCCCGGCGCGGGCGATCTCCTCGAGGAGATCTCAAGTATTGATGCTCGCGCACCCTTTCGAAATATGGAGA
CGCCTGGTGGCTTCCGAATGTCTGTCGCAATGACGAACTGCGGGACGTTGGGTTGGACGACGGATCGGAGTGGCTACCGT
TACACCGTCATCGATCCGGACACAGCCAAGCGTTGGCCGGCAATGCCGGGGTTGCTGTCAGCACTCGCGAACAACGCTGC
GGCAGTCTGCGGTTTCCCTGATTTTGAGCCCGATGCTTGTCTGATTAATCGGCACCTGCCCGGGGCGCGCCTCTCATTGC
ATCAGGACAAGGACGAGCGCGATCTCAACGCACCAATCGTGTCGGTGTCTCTAGGAATGACAGCCATATTCCTGTTTGGT
GGGCACGCGCGCGGTGATAGCGCCGAACGCGTTCTCCTCCGCCATGGAGACGTTGTTGTATGGGGAGGCGTCGATCGAAT
GCGGTATCACGGGATACTGCCACTGAAGGACGTCCCGCATCCGTTACTTGGTAGCCAACGGATAAACCTCACGTTCAGGA
AGGCCGGATAA

Upstream 100 bases:

>100_bases
CGATTCCGTGTCACCGAGTCGTTCGTCGCGATGGATCGGTATCCGGTTATCGCTGGGGGGTGGATCGCAAGCGTGCACTG
CTCTCGAAAGAGAGCGAGCA

Downstream 100 bases:

>100_bases
GGATACAGTGGCGCTGCACTCACGACGGCCCGTTCAAGGGCAGTCGTGACCACTATCGCGTGCGCACCACGCACGCGATC
AGCCGTGTGCATTCAAGAGA

Product: DNA-N1-methyladenine dioxygenase

Products: NA

Alternate protein names: Alkylated DNA repair protein AlkB [H]

Number of amino acids: Translated: 216; Mature: 215

Protein sequence:

>216_residues
MSTFDKDRYWSVDGREILGENAFILRGFALPGAGDLLEEISSIDARAPFRNMETPGGFRMSVAMTNCGTLGWTTDRSGYR
YTVIDPDTAKRWPAMPGLLSALANNAAAVCGFPDFEPDACLINRHLPGARLSLHQDKDERDLNAPIVSVSLGMTAIFLFG
GHARGDSAERVLLRHGDVVVWGGVDRMRYHGILPLKDVPHPLLGSQRINLTFRKAG

Sequences:

>Translated_216_residues
MSTFDKDRYWSVDGREILGENAFILRGFALPGAGDLLEEISSIDARAPFRNMETPGGFRMSVAMTNCGTLGWTTDRSGYR
YTVIDPDTAKRWPAMPGLLSALANNAAAVCGFPDFEPDACLINRHLPGARLSLHQDKDERDLNAPIVSVSLGMTAIFLFG
GHARGDSAERVLLRHGDVVVWGGVDRMRYHGILPLKDVPHPLLGSQRINLTFRKAG
>Mature_215_residues
STFDKDRYWSVDGREILGENAFILRGFALPGAGDLLEEISSIDARAPFRNMETPGGFRMSVAMTNCGTLGWTTDRSGYRY
TVIDPDTAKRWPAMPGLLSALANNAAAVCGFPDFEPDACLINRHLPGARLSLHQDKDERDLNAPIVSVSLGMTAIFLFGG
HARGDSAERVLLRHGDVVVWGGVDRMRYHGILPLKDVPHPLLGSQRINLTFRKAG

Specific function: Dioxygenase that repairs alkylated DNA and RNA containing 3-methylcytosine or 1-methyladenine by oxidative demethylation. Has highest activity towards 3-methylcytosine. Has lower activity towards alkylated DNA containing ethenoadenine, and no detectable a

COG id: COG3145

COG function: function code L; Alkylated DNA repair protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Fe2OG dioxygenase domain [H]

Homologues:

Organism=Homo sapiens, GI87298840, Length=124, Percent_Identity=32.258064516129, Blast_Score=74, Evalue=7e-14,
Organism=Escherichia coli, GI1788541, Length=202, Percent_Identity=61.3861386138614, Blast_Score=251, Evalue=3e-68,
Organism=Drosophila melanogaster, GI45555401, Length=125, Percent_Identity=33.6, Blast_Score=75, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004574 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 23681; Mature: 23550

Theoretical pI: Translated: 7.13; Mature: 7.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTFDKDRYWSVDGREILGENAFILRGFALPGAGDLLEEISSIDARAPFRNMETPGGFRM
CCCCCCCCEECCCCHHHHCCCEEEEEEECCCCHHHHHHHHHHCCCCCCCCCCCCCCCEEE
SVAMTNCGTLGWTTDRSGYRYTVIDPDTAKRWPAMPGLLSALANNAAAVCGFPDFEPDAC
EEEEECCCCCCCCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHCCCEEEECCCCCCCCEE
LINRHLPGARLSLHQDKDERDLNAPIVSVSLGMTAIFLFGGHARGDSAERVLLRHGDVVV
EEECCCCCCEEEECCCCCCCCCCCCEEEEECCCEEEEEECCCCCCCHHHEEEEECCCEEE
WGGVDRMRYHGILPLKDVPHPLLGSQRINLTFRKAG
ECCCCHHHHCCCCCHHCCCCCCCCCEEEEEEEEECC
>Mature Secondary Structure 
STFDKDRYWSVDGREILGENAFILRGFALPGAGDLLEEISSIDARAPFRNMETPGGFRM
CCCCCCCEECCCCHHHHCCCEEEEEEECCCCHHHHHHHHHHCCCCCCCCCCCCCCCEEE
SVAMTNCGTLGWTTDRSGYRYTVIDPDTAKRWPAMPGLLSALANNAAAVCGFPDFEPDAC
EEEEECCCCCCCCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHCCCEEEECCCCCCCCEE
LINRHLPGARLSLHQDKDERDLNAPIVSVSLGMTAIFLFGGHARGDSAERVLLRHGDVVV
EEECCCCCCEEEECCCCCCCCCCCCEEEEECCCEEEEEECCCCCCCHHHEEEEECCCEEE
WGGVDRMRYHGILPLKDVPHPLLGSQRINLTFRKAG
ECCCCHHHHCCCCCHHCCCCCCCCCEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3536913; 9097040; 9278503; 3887409; 7928996 [H]